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    Cloning and Functional Verification of SiCIPK21 Gene in Foxtail Millet
    DU YanWei, YAN XiaoGuang, ZHAO JinFeng, JIA SuQing, WANG GaoHong, YU AiLi, ZHANG Peng
    Scientia Agricultura Sinica    2024, 57 (22): 4416-4430.   DOI: 10.3864/j.issn.0578-1752.2024.22.003
    Abstract1174)   HTML28)    PDF (6709KB)(1147)       Save

    【Objective】The Ca2+-CBL-CIPK signaling pathway has important functions in plant response to abiotic stresses. By cloning the SiCIPK21 gene and studying its function under stress conditions, we provide a key candidate gene and theoretical basis for molecular breeding of foxtail millet with stress tolerance.【Method】Bioinformatics was used to analyze the cis-acting elements in the promoter region of this gene and predict the interactions between this protein and AtCBLs in Arabidopsis thaliana. SiCIPK21 was cloned by PCR, and a fusion expression vector was constructed for transient expression in tobacco to determine the subcellular localization. foxtail millet cv. Yugu 1 was used as material, and specifically amplified part of the SiCIPK21 gene fragment from Yugu 1 leaves, and recombinant vector VIGS-pTRV2-SiCIPK21 was constructed, using the phytoene desaturase gene (SiPDS) as the indicator gene, and seedlings of foxtail millet at the two-leaf stage were selected and infiltrated by cotyledon injection to investigate the role of SiCIPK21 under salt stress (250 mmol·L-1 NaCl) by using virus-induced gene silencing (VIGS) technology. T3 generation transgenic lines were obtained by overexpressing SiCIPK21 in Arabidopsis thaliana. Phenotypes at germination were analyzed under different concentrations of NaCl (150/175 mmol·L-1), mannitol (300/400 mmol·L-1) and ABA (0.25/0.5 μmol·L-1) treatments, and salt and drought tolerant phenotypes at seedling stage were also analyzed.【Result】Subcellular localization revealed that SiCIPK21 was located in the nucleus. The protein SiCIPK21 might interact with AtCBL2, AtCBL3, AtCBL4, AtCBL9, and AtCBL10 in Arabidopsis thaliana. The promoter region of SiCIPK21 contained adverse response elements, suggesting that SiCIPK21 may participate in the adverse responses. The VIGS gene silencing demonstrated that SiCIPK21-silenced foxtail millet plants had increased sensitivity to salt stress than the control plants. Three independent T3 generation Arabidopsis thaliana overexpression lines (2#, 3# and 6#) were obtained by genetic transformation. Overexpression lines showed significantly higher germination rate, germination speed, green cotyledon unfolding rate, root length and fresh weight than the wild-type plants (WT) at different concentrations of NaCl (150/175 mmol·L-1), mannitol (300/400 mmol·L-1) and ABA (0.25/0.5 μmol·L-1). Moreover, phenotypic analysis of salt and drought tolerance in Arabidopsis seedlings showed that overexpression lines had significantly higher survival rates and chlorophyll contents than WT.【Conclusion】SiCIPK21 is a positive regulator of plant response to salt and drought stresses, which makes it a candidate gene for improving stress tolerance by molecular breeding in foxtail millet.

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    Oat Plant Height Estimation Based on a Dual Output Regression Convolutional Neural Network
    ZHANG JianLong, XING WenWen, YE ShaoBo, ZHANG Chao, ZHENG DeCong
    Scientia Agricultura Sinica    2024, 57 (20): 3974-3985.   DOI: 10.3864/j.issn.0578-1752.2024.20.003
    Abstract1027)   HTML13)    PDF (5012KB)(131)       Save

    【Objective】 Oat plant height affects the productivity per plant and the yield per unit area together with planting density. This study explores automatic, real-time, and precise methods for acquiring oat plant height in a field environment, aiming to provide technical references for the automated field management of oat. 【Method】 Firstly, an oat depth image acquisition system was built based on Intel RealSense D435 depth camera and LabVIEW software development platform. Taking Oat ‘Pinyan No. 4’ as the research object, 26 376 modeling data and 2 205 test data were obtained during the whole oat growth process. The average and highest plant height of oats in each depth image were measured with a scale. The quantity of modeling data and test data in each height range of oat plant was relatively balanced. The images were preprocessed by high restoration, grayscale and scaling. Each image was tagged with two labels, one for the average and one for the highest plant height of the oats in the image. Then, based on 8 classical convolutional neural network models, the last layer (classification layer) of each network model was removed, and two fully connected layers with single nodes and no activation function were added to construct the double output regression convolutional neural network estimation model. Mean square error (MSE) was used to evaluate the accuracy of each model in estimating oat plant height. Finally, based on the TensorFlow deep learning platform, Modified EfficientNet V2L was selected as the estimation model by 5-fold cross-validation using the modeling data. 【Result】 The generalization performance of Modified EfficientNet V2L model to estimate oat plant height was investigated using test data not involved in model training. The mean absolute error (MAE), root mean square error (RMSE) and mean relative error (MRE) to estimate oat average plant height were 2.30 cm, 2.90 cm and 4.4%, respectively. Meanwhile, the MAE, RMSE and MRE to estimate highest plant height was 2.24 cm, 2.82 cm and 4.1%, respectively. The average estimated time of the model was 52.14 ms. The accuracy of estimating crop plant height using this method was similar to that of existing methods. However, when estimating crop plant height used this method, once the estimation model was trained, the average and maximum crop plant height could be automatically estimated by inputting the pre-processed crop depth image, and the average estimation time could meet the real-time requirements of crop plant height acquisition. The relative errors in estimating average plant height and maximum plant height of oat showed a general decline trend with the increase of crop plant height. This might be because when crop plant height was low, the estimated results were more affected by soil fluctuation. The results of feature map visualization showed that the model could estimate plant height according to the height and contour of oat in depth image. Finally, an oat plant height estimation system was built based on 2023 Q1 version of LabVIEW software development platform. After depth camera acquiring oat depth images, the system could accurately estimate average and highest oat plant heights in real time without manual intervention, and the average estimation time was less than 0.1 seconds. The system could be used for crops irrigation and fertilization management. It could also be installed on the tractors to control the height of a sprinkler head during spraying, and to adjust the height of a cutting table during harvesting. 【Conclusion】 The depth image and double output regression convolutional neural network could be used to estimate oat plant height, and the accuracy could meet the production demand, so this method provided a basis for field management of oat crops.

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    Isolation and Identification of Soybean Rhizosphere Growth-Promoting Bacteria and Their Salt Tolerance and Growth-Promoting Effects
    SHAO JiaZhu, LÜ Wen, LIAO XinLin, YUAN XinYu, SONG Zhen, JIANG DongHua
    Scientia Agricultura Sinica    2024, 57 (21): 4248-4263.   DOI: 10.3864/j.issn.0578-1752.2024.21.007
    Abstract870)   HTML53)    PDF (6181KB)(2206)       Save

    【Objective】Plant growth-promoting rhizobacteria (PGPR) can effectively promote plant growth through long-term mutualistic symbiosis with plants. The objective of this study is to screen and identify rhizosphere growth-promoting actinomycetes from soybean rhizosphere soil and clarify their growth-promoting mechanisms, and to lay a foundation for the development and utilization of microbial agents. 【Method】Different actinomycetes were collected using soil dilution coating method and plate streak inoculation method. The growth-promoting characteristics of isolates were analyzed using phosphorus solubilization, potassium solubilization, and siderophore assay media. Through microscopic observation, physiological and biochemical experiments, and multi-gene sequence analysis, the target growth-promoting actinomycetes were identified. Different salt concentrations (0-10%) were set to determine the salt tolerance of the target isolate. The growth-promoting ability of the isolate on soybean seedlings was detected by pot experiments and whether spraying the culture medium of the isolate under salt stress conditions could improve the stress resistance of soybean plants was evaluated. 【Result】Thirteen PGPR isolates were screened and obtained. Among then, the Sg-7 isolate not only had strong abilities to dissolve phosphorus, potassium, siderophore, and IAA production, but also effectively antagonized Pseudomonas syringae pv. glycinea (Psg). Based on morphological characteristics, biochemical, and multi-gene analyses, the soybean rhizosphere growth-promoting bacterium Sg-7 was identified as Streptomyces griseocarneus. The Sg-7 strain had certain salt tolerance characteristics, with a maximum tolerance to 7% NaCl salt concentration. Pot experiments showed that the diluted culture fluid after irrigation could effectively promote the germination of soybean seeds and improve growth indicators such as root length, stem diameter, fresh weight, and leaf area. Under 150 mmol·L-1 salt stress, irrigation with a 50× dilution of the Sg-7 culture fluid could effectively enhance SOD enzyme activity of soybean leaf, which was 1.84 times that of the salt stress treatment group. The CAT enzyme activity after irrigation with 100× dilution of culture fluid was 4.33 times higher than that of the salt stress treatment group. The POD enzyme activity after irrigation with 10× dilution of culture fluid was 1.10 times that of the salt stress treatment group. The culture fluid of Sg-7 strain could also enhance the root activity of soybean plants and promote the increase of carotenoid content in leaves to resist stress, which was 3.05 and 1.12 times that of salt stress treatment group, respectively. 【Conclusion】S. griseocarneus Sg-7 strain is a potential plant rhizosphere growth-promoting bacterium with broad development potential and practical application value.

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    Comparison of the Genome Sequence Polymorphisms Between the Main Naked Barley Varieties Kunlun 14 and Kunlun 15 in Qinghai Province
    XU JinQing, BIAN HaiYan, CHEN TongRui, WANG Lei, WANG HanDong, YOU En, DENG Chao, TANG YouLin, SHEN YuHu
    Scientia Agricultura Sinica    2024, 57 (21): 4192-4204.   DOI: 10.3864/j.issn.0578-1752.2024.21.003
    Abstract813)   HTML17)    PDF (2478KB)(414)       Save

    【Objective】At present, Kunlun 14 and Kunlun 15 are the main varieties of the naked barley in Qinghai Province, and also the important backbone parents in the breeding of the naked barley. The genomic sequences of Kunlun 14 and Kunlun 15 were compared to provide references for the trace of important character regions/loci, pedigree analysis and their utilization in molecular design breeding of the naked barley. 【Method】In this study, the agronomic and grain traits of Kunlun 14 and Kunlun 15 were investigated in the field, and the whole genome resequencing were performed (sequencing depth ≥15×). The sequence differences of copy number variation (CNV), single nucleotide polymorphism (SNP) and insertion/deletion (InDel) were compared between the two varieties. According to the SNP distribution patterns, the polymorphism hotspot and genetic similar regions between them were identified. The mutation types of polymorphism hotspot and genetic similar regions were compared and analyzed. The gene enrichment analysis was carried out in the specific CNV regions and the polymorphism hotspots regions of the two varieties. 【Result】In addition to the plant height and peduncle internode length, Kunlun 14 and Kunlun 15 had high similarity in important agronomic and grain traits. Compared with the barley reference genome of Morex, the two varieties had a common CNV variation interval of 83 Mb, and the length of cultivar-specific CNV variation interval in Kunlun 14 and Kunlun 15 was 37 Mb and 38 Mb, respectively. There were 564 genes in the CNV region specific to Kunlun 14, which were significantly enriched in 15 GO terms, while 519 genes were in the CNV region specific to Kunlun 15 and were significantly enriched in 7 GO terms. Based on the SNP distribution patterns, 1 706 Mb polymorphism hotspots and 2 411 Mb sequence similarity intervals of Kunlun 14 and Kunlun 15 were identified at the whole genome level, and the polymorphism hotspots were mainly distributed on 3H, 6H and 7H. The polymorphism hotspots regions contained 16 768 genes, whose functions were mainly related to plant growth and development. There was no significant difference between polymorphism hotspot regions and genetic similar regions in SNP variation type, InDel length distribution and the proportion of mutations affecting coding function. The SNPs and InDel mutations in polymorphism hotspot and genetic similar regions were mainly missense mutations and followed by synonymy mutations. 【Conclusion】The field phenotypes of Kunlun 14 and Kunlun 15 were similar. At the whole genome level, a total of 75 Mb CNV variation regions between them and the polymorphism hotspot regions were mainly distributed at 3H, 6H and 7H.

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    Application Status and Development Suggestion of Direct-Seeding Rice Cultivation in China
    LIAO Ping, WENG WenAn, GAO Hui, ZHANG HongCheng
    Scientia Agricultura Sinica    2024, 57 (24): 4854-4870.   DOI: 10.3864/j.issn.0578-1752.2024.24.003
    Abstract710)   HTML52)    PDF (728KB)(413)       Save

    With the continuous improvement in rice cultivation techniques, China has maintained a high rice production level of about 210 million tons over the past decade. Direct-seeding rice cultivation technology, recognized for its efficiency and simplicity, has been favored by Chinese farmers. However, controversies persist regarding direct-seeding rice compared to transplanted rice in national-scale production. Thus, this study employed meta-analysis techniques to quantify disparities in grain yield, economic benefit, rice quality, lodging characteristic, and greenhouse gas emissions between direct-seeding rice and transplanted rice. Our results indicated that direct-seeding rice significantly reduced grain yield by an average of 6.3% relative to transplanted rice, which was main due to the reduced total spikelet (-3.8%) and filled-grain percentage (-1.8%). In different planting systems in China, the yield of direct-seeding rice had significantly decreased compared to transplanted rice, and the direct-seeding rice-induced reductions in yield of single rice (-10.9%) and late rice (-13.1%) were higher than those of middle rice (-4.8%) and early rice (-4.4%). The grain yield reductions for direct-seeding rice were from 10% to 20% in Jilin, Liaoning, Xinjiang, Ningxia, Shandong, Jiangsu, and Zhejiang provinces, meanwhile Heilongjiang and Jiangxi provinces saw reductions of 5% to 10%, but it had no significant effect in other provinces. Direct-seeding rice resulted in comparable net economic return relative to transplanted rice (p> 0.05). Direct-seeding rice reduced milled rice rate (-3.1%) and gel consistency (-3.5%), improved appearance quality (chalkiness percentage and chalkiness degree, which decreased by 25.3% and 22.5%, respectively), whereas no significant effects were observed on nutrition quality and taste value. Direct-seeding rice increased lodging index at base of the first (+12.4%) and third (+10.3%) internodes, but not at the second internode, indicating an increase in risk of lodging relative to transplanted rice. In terms of greenhouse gas emissions, direct-seeding rice fields showed reductions in methane emissions (-42.8%), global warming potential (-36.2%), and greenhouse gas intensity (-41.1%) compared to transplanted rice fields, while promoting nitrous oxide emissions (+29.1%). In addition, a review was recounted on nitrogen utilization and its loss, water and energy use efficiency, and weed incidence. Finally, the recommendations for the future advancement of direct-seeding rice were proposed, main focusing on rice variety breeding, rice cultivation technique optimization, rice planting area layout, as well as policies and services with the goal of technological innovation and regionalized application of direct-seeding rice cultivation technology in China.

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    A Retrieval System for Great Soil Groups from China’s Provisional Soil Classification System for the 3rd National Soil Census
    LONG HuaiYu, LU ChangAi, JI HongJie, ZHANG RenLian
    Scientia Agricultura Sinica    2024, 57 (21): 4264-4275.   DOI: 10.3864/j.issn.0578-1752.2024.21.008
    Abstract680)   HTML52)    PDF (482KB)(1204)       Save

    【Objective】The State Council’s Office of the Leading Group for the 3rd National Soil Census released China’s Provisional Soil Classification System for the 3rd National Soil Census (Trial), abbreviated as 3PSC-T, in 2023, without retrieval system, which is unable to address long-standing questions such as “one soil having multiple names” and “different soils sharing the same name”. Therefore, the retrieval system for great soil groups need be built. 【Method】The retrieval indicators for each great soil group were extracted from the soil classification basis in 3PSC-T by using multiple methods, such as referring to existing diagnostic horizons and characteristics, refining existing diagnostic horizons and characteristics, simplifying descriptive statements, and inducting characteristic soil layers. Four principles for retrieval order were set up, which included prioritizing the importance of agriculture, prioritizing ease over difficulty in identification, narrowing down meaning, clustering similar types, and reflecting the spatial evolution and development patterns of soil. 【Result】Consequently, the retrieval system for 62 great soil groups in 3PSC-T was established, and the retrieval order was: 1 Artificial soils, 2 Paddy soils, 3 Vegetable cultivated soils, 4 Irrigation silting soils, 5 Irrigated desert soils, 6 Solonetzs, 7 Frigid plateau solonchaks, 8 Desert solonchaks, 9 Acid sulphate soils, 10 Coastal solonchaks, 11 Meadow solonchaks, 12 Volcanic soils, 13 Frigid frozen soils, 14 Purplish soils, 15 Phospho-calcic soils, 16 Lithosols, 17 Skeletal soils, 18 Takyr, 19 Neo-alluvial soils, 20 Aeolian soils, 21 Loessial soils, 22 Red primitive soils, 23 Limestone soils, 24 Bleached baijiang soils, 25 Podzolic soils, 26 Brown coniferous forest soils, 27 Dark-brown earths, 28 Brown earths, 29 Yellow-brown earths, 30 Yellow-cinnamon soils, 31 Gray forest soils, 32 Black soils, 33 Gray-cinnamon soils, 34 Cinnamon soils, 35 Torrid red soils, 36 Chernozems, 37 Castanozems, 38 Castano-cinnamon soils, 39 Black loessial soils, 40 Brown calcic soils, 41 Sierozems, 42 Gray desert soils, 43 Gray-brown desert soils, 44 Brown desert soils, 45 Mountain meadow soils, 46 Shrubby meadow soils, 47 Shajiang black soils, 48 Meadow soils, 49 Fluvo-aquic soils, 50 Bog soils, 51 Peat soils, 52 Latosols, 53 Latosolic red earths, 54 Red earths, 55 Yellow earths, 56 Dark felty soils, 57 Felty soils, 58 Frigid calcic soils, 59 Cold calcic soils, 60 Cold brown calcic soils, 61 Frigid desert soils, 62 Cold desert soils, and 63 Cambosol. 【Conclusion】It was found that some soil groups in 3PSC-T had overlapping or inclusive associations with one another. This necessitates the creation of a retrieval system to effectively address the issue of “one soil having multiple names” and “different soils sharing the same name”. In general, the majority of the soil groups in 3PSC-T had the ability to provide retrieval indicators through the examination of profile morphology as well as physical and chemical properties of the soil. Nevertheless, the soil groups within the alpine soil order deviated from the classification principles of the overall classification system. This deviation posed challenges in differentiating them based on profile morphology and soil physicochemical properties. Instead, distinguishing these soil groups required consideration of non-soil properties, such as distribution area and altitude. Further efforts were required to enhance the categorization of soil types in 3PSC-T. It is suggested to incorporate specific groups and to establish a new group called Cambosol.

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    Aroma Quality Analysis of Guangdongxiangshui Lemon Based on Molecular Sensory Technology
    ZHANG SiNing, ZHANG XingRui, WU DongXuan, KANG JingBo, CHEN XiaoLin, GENG LiJun, YIN GuangMin, CHEN JiaJing, GAO JunYan, CAI ZhongHu, LIU Yuan, XU Juan
    Scientia Agricultura Sinica    2025, 58 (1): 141-155.   DOI: 10.3864/j.issn.0578-1752.2025.01.011
    Abstract557)   HTML21)    PDF (3223KB)(4217)       Save

    【Objective】Tea drinks made from Guangdongxiangshui lemon is popular among consumers, but its aroma profile, main aroma-active compounds and corresponding key biosynthesis genes still need to be analyzed. In this study, integrated molecular sensory technology including metabolomics and sensory evaluation was used to explore the material basis and corresponding key genes for the aroma quality of Guangdongxiangshui lemon, aiming at laying a foundation of the citrus aroma quality. 【Method】The volatiles of Guangdongxiangshui lemon from three areas, including Weishan, Yunfu and Wuzhou, were detected via GC-MS, and the aroma quality were evaluated by sensory evaluation panels. The aroma active compounds of Guangdongxiangshui lemon were identified by GC/O-MS combined with aroma extract dilution analysis (AEDA), aroma activity value (OAV) analysis and aroma recombination experiment, and then the characteristic aroma compounds were further identified. Based on gene family analysis, the genes related to aroma formation were illustrated. 【Result】A total of 40, 21 and 33 volatiles were identified in the flavedo, pulp and slices of Guangdongxiangshui lemon, respectively. The samples with the highest volatile content in flavedo and pulp tissues were from Weishan and Yunfu, respectively. In terms of aroma attributes, the aroma intensity of Guangdongxiangshui lemon slices from Weishan and Yunfu was significantly higher than that of Wuzhou. The whole fruit aroma quality of samples from Wuzhou was better than that of samples from other origins. Solvent-assisted flavor evaporation (SAFE) and other methods were used to extract the essential oil of Guangdongxiangshui lemon in Weishan, and 25 aroma active compounds were identified. Combined with OAV analysis and aroma recombination experiments, citronellal and citral were further identified as its characteristic aroma compounds, and the aroma flavor wheel was further constructed. Based on the conserved domain and Blast comparison, 52 terpene synthase (TPS) genes were identified in Guangdongxiangshui lemon genome, which might be involved in the aroma compounds synthesis. 【Conclusion】The volatile spectrum and sensory evaluation showed that the aroma quality of Guangdongxiangshui lemon slices from Weishan were outstanding. A total of 25 aroma active compounds were identified. Among them, citronellal and citral were the characteristic aroma compounds of Guangdongxiangshui lemon, while d-limonene provided a background aroma. Combined with quantitative descriptive analysis and odor note collected via GC/O-MS, the aroma wheel of Guangdongxiangshui lemon was constructed with 15 aroma descriptors in 6 categories, including ‘fruity’ ‘woody’ ‘medicinal’ ‘spicy’ ‘floral’ and ‘grassy’. Furthermore, 52 TPS genes were mined in Guangdongxiangshui lemon genome, which might participate in the aroma compounds synthesis.

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    Research Progress on Seed Shattering of Rice
    LÜ ShuWei, TANG Xuan, LI Chen
    Scientia Agricultura Sinica    2025, 58 (1): 1-9.   DOI: 10.3864/j.issn.0578-1752.2025.01.001
    Abstract486)   HTML77)    PDF (2046KB)(948)       Save

    Seed shattering is a major factor limiting rice production, and breeding new rice varieties with moderate seed shattering is a key challenge faced by rice breeders worldwide. Rice is the most important cereal crop in China, plays a vital role for national food security. Seed shattering is one of the most important traits during rice domestication, and the abscission zone is the important region to control seed shattering. Compared with wild rice, cultivar has eliminated the seed shattering with partially developed abscission layer. Seed shattering not only has a direct impact on the yield, but also affects the way of its mechanical harvest. In order to breed rice varieties with moderate seed shattering in agricultural production, it is necessary to mine and utilize important seed shattering genes and introduce them into excellent rice varieties for genetic improvement, so as to breed new rice varieties suitable for mechanical harvesting with moderate seed shattering. Several seed shattering genes had been identified by map-based cloning, such as SH4/SHA1, qSH1, OsSh1/ObSH3, and their functional mechanisms had been analyzed. At the same time, new rice materials with moderate seed shattering have been successfully developed through CRISPR/Cas9 gene editing technology, gamma ray mutagenesis technology and gene introduction methods. Seed shattering has an important effect on grain yield and rice harvesting methods, in this paper, we reviewed the methods, physiologic basis, the identification of seed shattering genes and genetic mechanism of seed shattering in rice. At the same time, it is proposed that by using the important genes in excellent rice germplasm resources, could provide reference for exploring the mechanism of rice seed shattering, and breed new rice varieties suitable for mechanical harvesting with moderate seed shattering.

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    Structural Characteristics, Development Trends, and International Comparison of Greenhouse Gas Emissions in China’s Agri-Food System Under the Dual-Carbon Objectives
    NIU KunYu, GE RuoHao, CHEN MeiAn, JIN ShuQin, LIU Jing
    Scientia Agricultura Sinica    2024, 57 (21): 4290-4307.   DOI: 10.3864/j.issn.0578-1752.2024.21.010
    Abstract481)   HTML16)    PDF (1804KB)(137)       Save

    【Objective】This study adopted a comprehensive industry chain perspective to analyze the characteristics, structure, and trends of greenhouse gas emissions in the agricultural food system, contributing to the effective advancement of China’s low-carbon strategy. 【Method】Based on the latest statistics from the United Nations Food and Agriculture Organization, the statistical analysis, comparative analysis, and scenario analysis methods were used to analyze the trend and structural characteristics of greenhouse gas emissions in the agricultural food systems of China, the United States, Western Europe, India, Brazil, and globally from 1990 to 2020. Scenarios (baseline, 2 ℃ temperature control, and 1.5 ℃ temperature control) and specific stages were employed to predict China’s agricultural food system greenhouse gas emissions in 2050. This analysis formed the basis for identifying key areas for emission reduction and outlining achievable pathways. 【Result】Among the major economies mentioned, China was the only country with a significantly increasing per capita greenhouse gas emission in the agricultural food system, although it remained lower than that of developed economies. It was expected that, with the completion of industrialization, the advancement of low-carbonization in the energy industry, and increased difficulty in emission reduction in agricultural production, China’s share of greenhouse gas emissions from the agricultural food system in the total societal emissions would initially decrease and then rise. In this context, the food consumption stage was the fastest-growing segment in China’s agricultural food system greenhouse gas emissions, while the emission proportion from agricultural production was decreasing but still represented the largest share in the system. Unlike other major economies where enteric fermentation emissions were significantly higher, China’s agricultural production greenhouse gas emissions showed a diverse pattern, including enteric fermentation, rice cultivation, fertilizer application, and energy utilization. 【Conclusion】Future emission reduction efforts should focus on formulating comprehensive carbon-neutral strategies for the agricultural food system, low-carbonization of energy structures, promoting shifts in food consumption patterns, and advancing market-oriented emission reduction initiatives.

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    QTL Mapping and Candidate Gene Screening for Nitrogen Use Efficiency in Maize
    HAN XuDong, YANG ChuanQi, ZHANG Qing, LI YaWei, YANG XiaXia, HE JiaTian, XUE JiQuan, ZHANG XingHua, XU ShuTu, LIU JianChao
    Scientia Agricultura Sinica    2024, 57 (21): 4175-4191.   DOI: 10.3864/j.issn.0578-1752.2024.21.002
    Abstract468)   HTML36)    PDF (7416KB)(423)       Save

    【Objective】Genetic improvement for efficient utilization of maize nutrients represents a crucial method to ensure national food security. Exploring quantitative trait locus (QTL) and related candidate genes of nitrogen use efficiency can provide a theoretical basis for improving the efficiency of nitrogen fertilizer in maize and cultivating high-yield and high-efficiency maize varieties. 【Method】In this study, QTL mapping analysis in one recombinant inbred line (RIL) population constructed by KA105 and KB024 was performed for grain yield under two different nitrogen treatments, including the derived traits partial factor productivity from applied nitrogen (PFPN), low nitrogen tolerance coefficient (LNTC) and nitrogen agronomic efficiency (NAE). Concurrently, integrating the seedling transcriptome data of the parent KA105 under nitrogen treatment, differentially expressed genes were identified, and candidate genes associated with maize nitrogen use efficiency were mined through co-expression analysis. Subsequently, the selected candidate genes were validated using qRT-PCR. 【Result】Through mapping analysis, a total of 36 QTLs distributed across different chromosomes were detected, explaining 1.63% to 17.26% of the phenotypic variation. Among these, eight major QTLs with a phenotypic variation explanation rate exceeding 10% were identified, along with seven genetically stable QTLs commonly identified across different traits or environments. Notably, qNNGYP1 located on chromosome 1 has been repeatedly detected in previous studies, with a phenotypic explanation rate of up to 11.73%. Additionally, other QTLs (qNNGYP1, qPFPN1) co-located in this interval across different environments, suggesting it as a focal region for further investigation. Combining transcriptome data of seedlings under low nitrogen stress, 39 differentially expressed genes within these QTL intervals were identified, and 6 key genes were identified through co-expression network prediction. The result of qRT-PCR indicated that the expression trends of the candidate genes under both nitrogen treatments were consistent with the transcriptome data. Specifically, GRMZM2G366873 was involved in the regulation of auxin homeostasis and may participate in maize responses to low nitrogen stress, drought stress, and boron stress through auxin signal transduction, also regulating ear length. GRMZM2G414192 was involved in the response of the photosynthetic system to low nitrogen stress and was regulated by brassinosteroids. GRMZM2G414043 was associated with maize grain length and biomass, while GRMZM2G040642 may be involved in the long-distance signal transduction of nitrogen. 【Conclusion】In summary, a total of 36 QTLs were identified, distributed across chromosomes 1, 4, 5, 7, 8, and 9, including eight major QTLs (PVE>10%). The candidate genes GRMZM2G366873, GRMZM2G414192, GRMZM2G414043, and GRMZM2G040642 were identified as potential genes for maize nitrogen efficiency.

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    Development and Identification of Molecular Markers for Oil-Related Functional Genes and Polymerization Analysis of Excellent Alleles in Soybean
    WU ChuanLei, HU XiaoYu, WANG Wei, MIAO Long, BAI PengYu, WANG GuoJi, LI Na, SHU Kuo, QIU LiJuan, WANG XiaoBo
    Scientia Agricultura Sinica    2024, 57 (22): 4402-4415.   DOI: 10.3864/j.issn.0578-1752.2024.22.002
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    【Objective】Polymerizing soybean high oil genotypes aims at breeding varieties with higher oil content to improve economic efficiency and nutritional value. It is of great significance to increase agricultural output, reduce processing costs and meet global demand for vegetable oil growth.【Method】Glyma.18G027100 C2 gene family was identified by bioinformatic analysis method at the whole genome level. A total of 66 soybean C2 gene family members were identified, named GmC2-01.1-GmC2-20.2 according to chromosome position. Tissue pattern analysis revealed that 7 genes were highly expressed in grains among 66 C2 family genes (GmC2-03.6, GmC2-02.7, GmC2-07.2, GmC2-18.1, GmC2-18.4, GmC2-19.1 and GmC2-20.2). In order to analyze the effect sites of these genes on soybean oil content, SNP sites in the coding regions of these genes were obtained from SFGB database. Correlation analysis of oil content in two years showed that GmC2-18.1 has SNP loci that significantly affect oil content. The genetic diversity of GmC2-18.1 coding region was analyzed by 12 extreme materials. There was a G/A mutation at 2 038 273 bp in coding region of Wm82.a2.v1 version, which regulated seed oil content. It was preliminarily speculated that this gene played a role in seed development or nutrient accumulation. Then, SNP/InDel molecular markers were developed for GmC2-18.1-G/A gene combined with InDel natural allelic variation site 225 bp upstream of the start codon of GmSWEET39, T/C natural allelic variation site at 8 381 058 bp in coding region of GmST1, A/C natural allelic variation site at the third exon of 41 854 422 bp in coding region of GmMFT. 1 200 soybean germplasm resources from three ecological regions in China were identified by markers in 2 years.【Result】Analysis of variance showed that GmC2-18.1-G, GmSWEET39-Deletion, GmST1-T and GmMFT-A significantly increased oil content by 1.72, 1.95, 1.58 and 2.06 percentage points (P<0.01). The results showed that the average oil content of soybean seeds carrying GmC2-18.1-G, GmSWEET39-Deletion, GmST1-T and GmMFT-A high-oil allele type (PFAT-1) was 22.89%, which increased by about 4.5% compared with that carrying GmC2-18.1-A, GmSWEET39-Insertion, GmST1-C and GmMFT-C low-oil allele type (PFAT-14). 5 percentage points, the contribution rate to oil content is about 21.69%. 【Conclusion】Based on the markers developed above, 115 PFAT-1 high oil alleles were screened.

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    Effects on Pollen Release Related Traits of the Differential Genotypes Indica by High-Temperature Stress at Anthesis
    DU SiQi, WEN YuLun, NING LiXing, YIN XiaoYu, WANG ShuFen, SONG HaiYan, WANG ZhaoHai, LI WeiXing, LIAO JiangLin
    Scientia Agricultura Sinica    2025, 58 (10): 1867-1877.   DOI: 10.3864/j.issn.0578-1752.2025.10.001
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    【Objective】 To elucidate the causes of high-temperature stress inducing rice floret infertility, the present study analyzed the effects of high-temperature stresses on pollen release related traits including pollen grain swelling, anther dehiscence, pollen grain residue in anther and pollen grain deposition on the stigma of the differential genotypes Indica at anthesis. 【Method】Indica germplasms were sown in batches and cultivated in the Nanchang region, Jiangxi Province, China. The rice plants flowering at natural high-temperature environments on early August with 36.5-37.8 ℃ canopy temperature was used as treatments, and the rice plants flowering at suitable environments on middle September with 30.8-32.5 ℃ canopy temperature were used as controls. The pollen release related traits, such as pollen grain swelling, anther dehiscence, pollen grain residue in anther and pollen grain deposition on the stigma from treatments and controls, were detected and analyzed. 【Result】 After flowering under high-temperature stress, the rice germplasms Jiangxijiansimiao, Yuexiangzhan and Huangguangyouzhan show high-temperature tolerant at anthesis, and the floret fertility rates are 91.6%, 89.2% and 87.9%, respectively; while the germplasms Zhenfu, Yuzhenxiang, IR64 and Miyang46 show high-temperature sensitive at anthesis, and the floret fertility rates are just 55.2%, 60.3%, 61.1% and 73.2%, which are very significantly or significantly lower than that of its corresponding controls. Under high-temperature environments, the pollen grain swelling rates for the high-temperature sensitive germplasms Zhenfu, Yuzhenxiang, IR64 and Miyang46 are just 1.99%, 1.16%, 1.12% and 2.70%, which are very significant smaller than that of its corresponding controls; while the pollen grain swelling rates of the other germplasms show no significant difference between treatment and its corresponding control. Under high-temperature environments, the rates of anther dehiscence length in total anther length for the high-temperature sensitive germplasms Zhenfu, Yuzhenxiang, IR64 and Miyang46 are respective 66.0%, 45.4%, 48.7% and 63.6%, which are very significantly or significantly shorter than that of the corresponding controls, and the pollen grain residue are obvious more than that of the corresponding controls; while the anther dehiscence length rates and the pollen grain residue from the other germplasms show no significant difference between treatments and controls. After flowering under high-temperature environments, the average pollen grain number deposited on one stigma of the sensitive germplasms were about 20, which were significant less than that of the controls; while the average pollen grain number deposited on one stigma of the other rice germplasms show no significant difference between treatments and controls. 【Conclusion】 The high-temperature stresses inhibit the pollen grain swelling, effect the anther normal dehiscence, increase the pollen viscidity to impede the pollen grain releasing from anther and decrease the pollen grain number scattering on the stigma, inducing rice floret infertility and decreasing the seed set.

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    Cloning and Heat Tolerance Function of Wheat TaGRAS34-5A Gene
    DIAO DengChao, LI YunLi, MENG XiangYu, JI SongHan, SUN YuChen, MA XueHong, LI Jie, FENG YongJia, LI ChunLian, WU JianHui, ZENG QingDong, HAN DeJun, $\boxed{\hbox{WANG ChangFa}}$, ZHENG WeiJun
    Scientia Agricultura Sinica    2025, 58 (4): 617-634.   DOI: 10.3864/j.issn.0578-1752.2025.04.001
    Abstract449)   HTML78)    PDF (13208KB)(340)       Save

    【Objective】The GRAS family constitutes a unique class of plant-specific transcription factors that play a pivotal role in plant development and stress response. To elucidate the function of GRAS family genes in wheat heat tolerance,which can provide genetic resources and theoretical foundation for wheat heat-resistant breeding.【Method】A potential heat stress-responsive transcription factor gene, TaGRAS34-5A, was identified through transcriptome analysis of TAM107 and Chinese spring wheat seedlings under high-temperature conditions. Subsequently, a bioinformatics analysis was performed on TaGRAS34-5A, and a phylogenetic tree was constructed to elucidate its molecular characteristics. The expression pattern of TaGRAS34-5A under various stresses, including high temperature, abscisic acid (ABA), ethylene (ETH), and salicylic acid (SA) treatments, were examined using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) method. The subcellular localization of the TaGRAS34-5A protein was determined using wheat protoplast transient expression technique. Furthermore, the heat tolerance function of TaGRAS34-5A was validated using the heterologous expression system of Saccharomyces cerevisiae and the BSMV:VIGS (Barley stripe mosaic virus: Virus-Induced Gene Silencing) silencing technique. potential interacting proteins of TaGRAS34-5A were screened using yeast two-hybrid technology, and the heat tolerance function was verified, providing preliminary insights into its heat tolerance mechanism.【Result】TaGRAS34-5A, equipped with a characteristic GRAS domain and belongs to the GRAS transcription factor family, is localized to both the cell nucleus and cytoplasm. Bioinformatics analysis indicates that the TaGRAS34-5A promoter contains a large number of hormone response elements and light response elements, and it is most closely related to TaSCL14, OsGRAS23, and AtSCL14 in terms of phylogenetic relationships, suggesting its potential function in responding to oxidative stress. Its expression is upregulated under high-temperature, ethylene (ETH), abscisic acid (ABA), and salicylic acid (SA) treatments, peaking at 4, 6, 0.5, and 12 hours post-treatment, respectively, with the most significant induction observed under heat stress and SA. Functional assays in yeast demonstrated that heterologous expression of TaGRAS34-5A enhances the heat tolerance of the yeast. The results of BSMV:VIGS transient silencing experiment showed that after the 42 ℃ high-temperature treatment, TaGRAS34-5A silenced plants exhibited decreased chlorophyll content, reduced POD enzyme activity, increased cellular peroxidation, and decreased heat tolerance compared to the control. Preliminary studies on the heat tolerance mechanism suggest that TaGRAS34-5A exhibits strong transcriptional self-activation activity.it may modulate wheat heat tolerance by interacting with proteins such as the bZIP family transcription factor HBP-1b and the E3 ubiquitin ligase hel2, thereby regulating cellular redox homeostasis and detoxification processes, positively influencing the heat tolerance of wheat.【Conclusion】TaGRAS34-5A is induced by heat, ABA, ETH, and SA, and its encoded protein is located in the nucleus and cytoplasm. It exhibits transcriptional activation activity. Heterologous overexpression of TaGRAS34-5A enhances the heat tolerance of Saccharomyces cerevisiae. Silencing TaGRAS34-5A in wheat plants increases cellular peroxidation, decreases chlorophyll content, and reduces heat tolerance. TaGRAS34-5A may regulate the heat tolerance of wheat by modulating cellular redox state and detoxification processes.

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    CRISPR-Cas12a Gene Editing Technology and Its Application in Agricultural Production
    LUO Gang, CHENG YiYi, YANG Wen, XIAO YiMeng, YANG ChengXi
    Scientia Agricultura Sinica    2025, 58 (7): 1434-1450.   DOI: 10.3864/j.issn.0578-1752.2025.07.014
    Abstract440)   HTML52)    PDF (2162KB)(214)       Save

    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein (CRISPR- Cas) gene editing technology has not only revolutionized life sciences but also catalyzed transformative advancements in agriculture. As a critical branch of the CRISPR system, the CRISPR-Cas12a system exhibits unique molecular characteristics and distinct application potential in biological breeding and disease diagnosis compared to the classical CRISPR-Cas9 system. Unlike the Type II Cas9 system, the Type V Cas12a protein possesses a single RuvC-like nuclease domain, contrasting sharply with the dual HNH-RuvC nuclease domains of Cas9. Cas12a generates staggered double-strand breaks (DSBs) in target DNA while retaining the CRISPR RNA (crRNA) and Cas12a-formed "R-loop". The preservation of this R-loop constitutes the the structural basis for the collateral cleavage activity inherent to the CRISPR-Cas12a system, which underpins its utility in developing nucleic acid and small molecule detection technologies. Recognizing thymine-rich protospacer adjacent motifs (PAMs), CRISPR-Cas12a acts as a powerful complement to existing CRISPR-Cas systems. Its crRNA-dependent autonomous processing mechanism, distinct from the tracrRNA-dependent system of Cas9, offers superior advantages in multiplex gene editing. These features have driven breakthroughs in crop genetic improvement, including the successful development of disease-resistant and high-yield commercial crop varieties. In basic research, catalytically inactive Cas12a (dCas12a) fused with transcriptional regulators or epigenetic modifiers enables precise gene expression regulation without inducing DSBs. Furthermore, its integration with isothermal amplification techniques allows for visual disease detection.This review systematically introduced the CRISPR-Cas12a system from multiple perspectives: (1) classification of Type V Cas proteins, (2) mechanistic principles of Cas12a in bacterial immunity, and (3) functional domains of the Cas12a-crRNA complex. A comparative analysis between CRISPR-Cas12a and CRISPR-Cas9 was conducted across four dimensions: crRNA processing mechanisms, structural-functional features of Cas effectors, editing efficiency, and application scenarios. Additionally, the regulatory systems of CRISPR-dCas12a and CRISPR-dCas9 were evaluated regarding gene expression modulation, epigenetic editing, and base editing. The review also elucidated the molecular detection principles of CRISPR-Cas12a in targeting nucleic acids, proteins, and small molecules, as well as its agricultural applications in gene regulation, base editing, pathogen detection, disease diagnosis, and bio-breeding.With the emergence of safer non-DSB- dependent technologies such as prime editing, the CRISPR-Cas12a system was poised to play an increasingly vital role in crop precision breeding, livestock genetic improvement, and rapid clinical diagnostics. These advancemented promise innovative solutions to global food security challenges and infectious disease control, further cementing CRISPR-Cas12a as a cornerstone tool in agricultural biotechnology and molecular medicine.

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    Interactive Effects of Planting Density and Nitrogen Application Rate on Plant Grain Yield and Water Use Efficiency of Two Maize Cultivars
    TIAN LongBing, SHEN ZhaoYin, ZHAO XiaoTian, ZHANG Fang, HOU WenFeng, GAO Qiang, WANG Yin
    Scientia Agricultura Sinica    2024, 57 (21): 4221-4237.   DOI: 10.3864/j.issn.0578-1752.2024.21.005
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    【Objective】Reasonably increasing planting density combined with appropriate nitrogen (N) application rate is an important technical approach for increasing maize yield and resource use efficiency. Understanding the interactive effects of planting density and N rate on maize growth, evapotranspiration (ET) and water use efficiency (WUE) during the growing season, could provide a basis for improving its use efficiency when increasing planting density and controlling N input in maize production. 【Method】Field experiments were conducted during 2022 to 2023 in Jilin Province. Two maize cultivars, Liangyu 99 (LY99) and Demeiya 3 (DMY3), were used in this study. Three planting densities of 50 000, 70 000 and 90 000 plants/hm2 and four N application rates of 0, 100, 200 and 300 kg N·hm-2 were designed to investigate the effects of planting density and N application rate on grain yield and water productivity of different maize cultivars, as well as the dry matter (DM), soil water content, ET and WUE at various growth stages. 【Result】Planting density significantly affected DM and grain yield of maize, but the response trends varied between cultivars. Grain yields of LY99 with 70 000 plants/hm2 was 11.1% and 18.3% higher than that with 50 000 and 90 000 plants/hm2, respectively. The average yield of DMY3 planted with 70 000 plants/hm2 and 90 000 plants/hm2 was 10.5% and 9.3% higher than that of 50 000 plants/hm2, respectively. Nitrogen fertilization significantly increased DM and grain yield of maize, and also showed significant interactive effects with cultivar or planting density. Compared with N0, grain yields of LY99 were increased by 38.0% to 60.7% under N1, and the yield increases for DMY3 were 24.4% to 38.2%. Notably, the yield responses to N rates were more pronounced for LY99 compared with DMY3. For both cultivars, the yield differences between low N rate and high N rate enlarged with increasing planting density, with LY99 showing a more distinct performance. The water consumption and utilization of maize plants were also significantly affected by planting density, N rate and their interaction. During the growing season, the total ET of DMY3 continually increased with increasing density, while that of LY99 showed the highest values with 70 000 plants/hm2 among different densities. In each density condition, the ET of both cultivars increased with increasing N application rates. The WUE of maize plants showed complex responses to planting density and N rate at different growth stages, due to the varied annual precipitation and distribution patterns. The average increase of water productivity of LY99 under planting 50 000 and 70 000 plants/hm2 was 8.6% and 10.4% compared with 90 000 plants/hm2 respectively. DMY3 had the highest water productivity when planting 70 000 plants/hm2, which increased by 5.8% and 5.3% compared with 50 000 and 90 000 plants/hm2, respectively. The water productivity showed different responses to N rate among the three densities. In general, the difference of nitrogen application under low density was small, but it increased significantly under medium and high density. Compared wtih DMY3, LY99 showed higher increases for water productivity when N fertilizer was applied under medium and high density conditions. The correlation analysis showed that interactive effects of planting density and N rate significantly affected maize yield and water productivity by influencing the water utilization at various growth stages. 【Conclusion】Planting density and N rate had significant interactive effects on maize yield and water utilization in the rain-fed region of Northeast China. The two maize cultivars used in this study could obtain high grain yield and water productivity under a moderately higher density of 70 000 plants/hm2 combined with 200 kg N·hm-2 rate.

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    Re-Evaluation of China’s Agricultural Net Carbon Sink: Current Situation, Spatial-Temporal Pattern and Influencing Factors
    TIAN Yun, WANG XiaoRui, YIN MinHao, ZHANG HuiJie
    Scientia Agricultura Sinica    2024, 57 (22): 4507-4521.   DOI: 10.3864/j.issn.0578-1752.2024.22.010
    Abstract405)   HTML12)    PDF (624KB)(1773)       Save

    【Objective】Based on the current “dual carbon” strategic goal, this study aimed to clarify the current characteristics, spatio-temporal pattern and influencing factors of agricultural net carbon sink, so as to provide the important support for accelerating agricultural sink increase and emission reduction.【Method】Based on the scientific reconstruction of the index system, the carbon sink/carbon emission factor method was used to measure and analyze the current situation of China’s agricultural net carbon sink. Then the spatial autocorrelation model was used to discuss the spatial dependence and spatial heterogeneity. Finally, the least-squares method was used to analyze the main factors affecting the change of its intensity. 【Result】From 2005 to 2022, the total amount of agricultural net carbon sink in China was in an obvious upward trend, although there were some interannual fluctuations, and its evolutionary characteristics could be roughly divided into four stages, namely, “continuous rise”, “fluctuating decline”, “rapid rise”, and “slow rise”; the intensity of agricultural net carbon sink was also in an obvious upward trend, with only a slight difference in the trajectory of the evolution, and the difference in its growth rate could be roughly categorized into four stages: “continuous rapid growth”, “slow growth”, “fluctuating ups and downs”, and “slow growth”. 2022, the amount of agricultural net carbon sink had a large interprovincial difference, with Inner Mongolia being the first and Shanghai being the last, and compared with the year of 2005, all the provinces had a significant increase. In 2022, the net carbon sink intensity of agriculture would be the highest in Henan and the lowest in Qinghai, with all provinces showing different degrees of increase compared with 2005. China’s provincial agricultural net carbon sink intensity as a whole showed obvious spatial dependence, but there was also a local spatial clustering phenomenon, more than 70% of the provinces showed obvious spatial clustering characteristics, and the number of provinces located in the high-high clustering and the low-low clustering was approaching. The structure of arable land use, urbanization level, rural residents' income level and the internal industrial structure of agriculture all had a significant impact on the intensity of agricultural net carbon sink; specifically, the higher the ratio of sown area of grain crops, or the higher the urbanization rate, or the higher the income level of rural residents, or the larger the ratio of plantation industry to animal husbandry, the higher the intensity of net carbon sink in agriculture.【Conclusion】The total amount and intensity of China’s agricultural net carbon sink were in a fluctuating upward trend and there were obvious inter-provincial differences. The intensity of China’s agricultural net carbon sink showed obvious spatial dependence and spatial heterogeneity. The intensity of the agricultural net carbon sink was affected by the structure of arable land use, the level of urbanization, the level of rural residents' income, and the structure of the internal industries of agriculture. The measures should be taken to promote the enhancement of sink and emission reductions and to promote the enhancement of agricultural net carbon sink in agriculture, such as establishing a sound policy support system for the development of low-carbon agriculture, strengthening inter-provincial exchanges and cooperation, and increasing financial support for agriculture.

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    Yield Gain Analysis of Wheat Varieties in Sichuan from 2000 to 2020
    LUO JiangTao, ZHENG JianMin, DENG QingYan, LIU PeiXun, PU ZongJun
    Scientia Agricultura Sinica    2024, 57 (20): 3945-3956.   DOI: 10.3864/j.issn.0578-1752.2024.20.001
    Abstract404)   HTML50)    PDF (672KB)(250)       Save

    【Objective】Analyzing the yield and yield related traits of Sichuan wheat varieties from 2000 to 2020, providing reference for genetic improvement of yield in Sichuan wheat varieties. 【Method】From 2019 to 2022, a community trial design was used to measure the yield and related traits of 145 wheat varieties in Sichuan Province since 2001 to 2016, as well as 60 high-yield wheat varieties (Varieties with top yields in regional trials in Sichuan Province over the years) since 2000 to 2020. This data was used to analyze the trend of yield and yield related trait changes in Sichuan wheat cultivars cultivated from 2000 to 2020. 【Result】145 Sichuan wheat varieties from 2001 to 2016 have an average annual genetic gain of 37.20 kg·hm-2 or 0.66% in yield. Grain number per spike and effective spike number per unit area showed an increasing trend, while thousand grain weight and plant height showed a decreasing trend. Correlation analysis showed that effective spike number per unit area was positively correlated with yield. Path analysis showed that the continuous increase of effective spike number per unit area (annual increase 0.42×104/hm2 or 0.13%) was the main factor for the increase of yield potential of high-yielding varieties. The average annual yield genetic gain of 60 high-yield wheat varieties from 2000 to 2020 was 61.10 kg·hm-2 or 0.89%, the effective spike number per unit area showed an increasing trend, the plant height showed a decreasing trend, and the grain number per spike and thousand grain weight had almost no change. Correlation analysis shows that there was a significant positive correlation between yield and the number of effective ears per unit area. Path analysis showed that the continuous increase in effective spike number per unit area (with an average annual increase of 1.80×104/hm2 or 0.51%) was also a major factor in improving the yield potential of 60 high-yield wheat varieties in Sichuan from 2000 to 2020. 【Conclusion】The improvement and breeding of wheat yield heritage in Sichuan Province has made some progress, especially the improvement effect of high yield breeding is remarkable, and the yield level of wheat varieties in Sichuan Province is gradually increasing. The continuous increase in effective ears per unit area was the main factor for improving the yield potential of Sichuan wheat varieties. High grain number per spike and thousand grain weight are important foundations for high yield in Sichuan wheat, but their genetic improvement is in a bottleneck period. Increasing the effective spike number per unit area is the key to furtherly improve the yield of wheat in Sichuan.

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    Genome-Wide Identification of Soybean LOX Gene Family and the Effect of GmLOX15A1 Gene Allele on 100-Seed Weight
    WANG Wei, WU ChuanLei, HU XiaoYu, LI JiaJia, BAI PengYu, WANG GuoJi, MIAO Long, WANG XiaoBo
    Scientia Agricultura Sinica    2025, 58 (1): 10-29.   DOI: 10.3864/j.issn.0578-1752.2025.01.002
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    【Objective】Based on whole genome identification and analysis of soybean LOX gene family members, to understand the taxonomic evolutionary relationships of each member, to study the expression specificity of each gene member in different tissues and their response to abiotic stress, which provided a theoretical basis for further research on the molecular characteristics, evolutionary process, and function of the LOX gene family. 【Method】Based on the LOX protein sequences of rice and Arabidopsis species in Ensembl database, BLASTP alignment of homologous LOX protein sequences in soybean whole genome database was performed, and MEGA X software was used to construct a phylogenetic tree; Using website MEME for protein conserved motif analysis; Using online software GSDS 2.0 to analyze gene structure; Using TBtools for chromosome localization drawing; Analyze soybean LOX family replication genes using McscanX; Using the PlantCARE website to predict the promoter elements of soybean LOX gene family; Draw gene expression heatmaps of soybean under different tissues and abiotic stress using TBtools, and develop molecular markers for the excellent allele variant GmLOX15A1-G/A significantly correlated with 100-seed weight.【Result】A total of 43 LOX genes were identified in soybean, unevenly distributed on 13 chromosomes. Collinearity analysis indicates that the GmLOX gene has undergone extensive replication during the evolutionary process. Meanwhile, 39 different types of cis regulatory elements were detected in the LOX gene promoter, indicating that they may be involved in different pathways such as growth and development, light response, stress response, and hormone induction. Expression pattern analysis revealed that the LOX gene has different levels of expression in different tissues of soybean, indicating that members of this family have tissue and spatiotemporal expression specificity. Under drought stress conditions, the GmLOX gene was significantly differentially expressed in soybean roots and leaves (P<0.05). Among them, GmLOX3A3, GmLOX7A1, GmLOX20B1, GmLOX13A1, and GmLOX20A2 were significantly upregulated or downregulated in roots and leaves, suggesting that the GmLOX gene may play an important role in response to stress. At the same time, it was found that GmLOX15A1 is highly expressed in grain tissue and there is an excellent G/A allele variation in the seventh exon of the gene coding region. Molecular markers were developed for this variant site, and the correlation between different haplotypes of GmLOX15A1 and 100-seed weight was analyzed using 1 200 soybean germplasm resources from different ecological regions over a period of 2 years. The results showed that compared to the GmLOX15A1-A genotype, the average 100-seed weight of soybean germplasm carrying the GmLOX15A1-G allele gene increased by 2.33 g (P<0.001). 【Conclusion】A total of 43 members of the LOX family were identified in soybeans, which can be divided into 3 subfamilies. The promoter region of the GmLOX gene contains a large number of cis acting elements that respond to hormones and stress, playing different roles in drought stress response. Among them, GmLOX15A1 is highly expressed in grain tissue and there is an excellent G/A allele variation in the seventh exon of the coding region of this gene. Compared with the GmLOX15A1-A genotype, the average 100-seed weight of soybean germplasm carrying the GmLOX15A1-G allele gene is significantly increased by 2.33 g. This locus can be used as an excellent haplotype for genetic improvement of soybean grain size.

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    Genetic Composition Analysis of a New High Quality and High Yield Wheat Cultivar Taikemai33
    QI XiaoLei, WANG Jun, LÜ GuangDe, MU QiuHuan, MI Yong, SUN YingYing, YIN XunDong, QIAN ZhaoGuo, WANG RuiXia, WU Ke
    Scientia Agricultura Sinica    2024, 57 (22): 4391-4401.   DOI: 10.3864/j.issn.0578-1752.2024.22.001
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    【Objective】Taikemai33, derived from a cross between Zhengmai366 and Huaiyin9908, is a new released wheat cultivar with high quality, high yield, and excellent disease resistance, which has a broad genetic base, and a high potential for application in wheat production. The objective of this study is to dissect the genetic composition of Taikemai33 to provide information for parental selection to use this cultivar to develop more new wheat cultivars. 【Method】Taikemai33 and its pedigree parents including Zhengmai366, Huaiyin9908, Yumai47, PH82-2-2, Yumai13, Yumai 2 hao, Bainong3217, Yanda24, Xiannong39, Fengchan 3 hao and Funo were screened using the 55K wheat SNP chip to dissect the genomic composition of Taikemai33 to evaluate the genetic contributions of each parental line to Taikemai33. 【Result】The similarity coefficient between Taikemai33 and its pedigree parents ranged from 0.72 to 0.93, and the genetic composition of Taikemai33 was highly similar to Zhengmai366, the pedigree mother parent, with a genetic similarity coefficient of 0.93. SNP marker analysis showed that the pedigree parents contributed different proportion to the genome of Taikemai33, with the pedigree mother contributed 66.57%, whereas the pedigree father contributed 33.43%, indicating that Taikemai 33 inherits more genetic materials from the maternal lineage. Furthermore, the pedigree mother contributed 71.0%, 85.0% and 49.4% to subgenome A, B and D of Taikeimai33, whereas those were 29.0%, 15.0% and 50.6% contributed by the pedigree father. For each chromosome, the pedigree mother contributed more on chromosome 1A, 2A, 3A, 4A, 7A, 1B to 7B, 1D and 2D, whereas the pedigree father contributed more on chromosome 5A, 4D, 6D and 7D. The contributions of the pedigree parents on 6A, 3D and 5D were equal. Taikemai33 genotype map showed that the contribution loci of the pedigree mother were distributed in clusters on chromosome 1A, 5A, 7A, 2B, 7B, 2D, with those from the pedigree father were on chromosome 4A, 5A, 6D, 7D. Interestingly, among the polymorphic SNP loci, between Zhenmai366 and Huaiyin9908, Taikemai33 showed 109 loci that were absent in both parents, distributing on 19 chromosomes except 1A and 6A. Chromosome 4A, 2B, 6B and 7D of Taikemai33 confer most of the polymorphic SNPs in clusters with cluster number of 10, 9, 11, and 9. 【Conclusion】We constructed the genotype map and dissected the genetic composition of Taikemai33, determined the loci contributed by the pedigree parents and identified that Taikemai33 inherited more genetic materials from the pedigree mother and conferring some specific loci different with the pedigree parents.

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    The Impact of Diversified Crops and Wheat-Maize Rotations on Soil Quality in the North China Plain
    ZHANG SiJia, YANG Jie, ZHAO Shuai, LI LiWei, WANG GuiYan
    Scientia Agricultura Sinica    2025, 58 (2): 238-251.   DOI: 10.3864/j.issn.0578-1752.2025.02.003
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    【Objective】Based on the long-term experiment in the North China Plain (NCP), the differences in soil nutrient and aggregate nutrient distribution between diversified crops and wheat-maize rotation systems were investigated. Additionally, it provided a comprehensive evaluation of soil quality indices (SQI), offering a scientific basis for enhancing soil quality and productivity in the NCP. 【Method】Four diversified crop rotation systems were evaluated, including spring sweet potato-winter wheat-summer maize (Psw-WM), spring peanut-winter wheat-summer maize (Pns-WM), spring sorghum-winter wheat-summer maize (Ps-WM), with winter wheat-summer maize (WM-WM) serving as the control. The soil samples from the 0-40 cm depth were collected during the second rotation in 2022, at the flowering and harvesting stages of winter wheat. The soil enzymes activities, aggregate stability, organic matter, and concentrations of nitrogen, phosphorus, and potassium in soil and aggregates of different sizes (>2.00 mm, 0.50-2.00 mm, 0.25-0.50 mm, and <0.25 mm) were assessed. The SQI for each crop rotation system was then comprehensively evaluated. 【Result】Compared with WM-WM, the three other crop rotations increased soil inorganic nitrogen content. Psw-WM significantly enhanced organic matter in the 0-20 cm layer, total nitrogen in soil aggregates (>2.00 mm, 0-10 cm), and organic matter in soil aggregates (>2.00 mm and 0.50-2.00 mm, 0-10 cm), which also increased cellulase, catalase, and alkaline protease activities. Pns-WM improved organic matter in the 20-40 cm layer and available potassium in soil aggregates (0.25-0.50 mm and >2.00 mm, 10-20 cm), as well as organic matter in soil aggregates (0-10 cm, >2.00 mm and 10-20 cm, >0.50 mm), which also increased sucrase, urease, and alkaline protease activities. Psw-WM improved the stability of 0-10 cm soil aggregates, while Pns-WM improved the stability of 0-30 cm soil aggregates. Both Pns-WM and Psw-WM significantly improved the SQI, with Pns-WM showing a higher improvement than Psw-WM. The path analysis revealed that the average weight diameter (MWD) of aggregates was a direct and significant affecting SQI. It also had a significant indirect positive effect on SQI by influencing inorganic nitrogen. Additionally, the increased organic matter led to a higher proportion of large aggregates, which significantly affected SQI indirectly. 【Conclusion】Legume (peanut) and root crop (sweet potato) rotations with wheat-maize rotations could significantly improve soil quality and enhance the soil nutrient supply capacity in the NCP.

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    Cloning of the Promoters and Analysis of Expression Patterns of Maturity Genes E1 and E2 in Soybean
    LIU LuPing, HU XueJie, QI Jin, CHEN Qiang, LIU Zhi, ZHAO TianTian, SHI XiaoLei, LIU BingQiang, MENG QingMin, ZHANG MengChen, HAN TianFu, YANG ChunYan
    Scientia Agricultura Sinica    2025, 58 (5): 840-850.   DOI: 10.3864/j.issn.0578-1752.2025.05.002
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    【Objective】Maturity time is an essential phenotypic measure of ecological adaptability of soybean and an important trait related to its yield formation. The study of promoters and expression patterns of major maturity genes E1 and E2 would provide basis for the study of gene function and molecular regulatory network of maturity time and lay foundation for adaptability improvement and yield increase in soybean.【Method】The promoter sequences of major maturity genes E1 and E2 were analyzed through the promoter cis-element analysis website PlantCARE, and the important regulatory elements were detected. The promoters of E1 and E2 were cloned, the GUS vectors were constructed, and transformation of Arabidopsis was performed to detect GUS activity in different tissues and organs of transgenic plants. Under low light and strong light conditions, the expression levels of E1 and E2 were compared between long day and short day conditions. The expression levels of E1 and E2 were detected in soybean varieties of different maturity groups, which is for the analysis of correlation between expression levels and maturity time of soybean varieties.【Result】Both E1 and E2 promoters contained multiple photoresponsive elements such as AE-box, Box4 and G-box, E1 promoter also contained auxin-response, abolic acid-response elements, and E2 promoter also contained low temperature-response, drought-response elements and meristem expression elements. In GUS activity detection of transgenic Arabidopsis, E1 promoter had strong transcriptional activity in all organs of the plant, and transcriptional activity of E2 promoter in fibrovascular tissues of seedling hypocotyl, leaf and root was relatively strong. Under both low light and strong light conditions, the expression level of E1 was significantly higher in long day than in short day. Under low light conditions, the expression level of E2 was higher in short day than in long day. Under strong light conditions, the expression level of E2 was higher in long day than in short day. With the increase of maturity time of different soybean varieties, expression level of E1 increased gradually, while E2 expression level did not change regularly.【Conclusion】The promoter of E1 gene was a widely expressed promoter, and its expression level was significantly regulated by photoperiod and significantly correlated with the maturity time of soybean varieties. The promoter of E2 was strongly expressed in vascular tissues of various organs, the photoperiodic regulation mode of this gene was different under strong light and low light conditions, and there was no significant correlation between expression level of E2 and maturity time.

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    The Function of OsDREB1J in Regulating Rice Grain Size
    WEI Ping, PAN JuZhong, ZHU DePing, SHAO ShengXue, CHEN ShanShan, WEI YaQian, GAO WeiWei
    Scientia Agricultura Sinica    2025, 58 (8): 1463-1478.   DOI: 10.3864/j.issn.0578-1752.2025.08.001
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    【Objective】 The AP2/ERF (APETALA2/ethylene responsive factor) superfamily is a group of transcription factors that play important regulatory roles in plant growth and development, as well as in response to adverse environmental stressors. The AP2/ERF transcription factors are widely present and have many members in plants. Exploring the function of AP2/ERF family gene on grain size provides important genetic resources for regulating grain shape in rice. 【Method】OsDREB1J gene (LOC_Os08g43200) was cloned by homologous recombination, and its basic characteristics, tissue expression characteristics, and the relative expression patterns under plant hormones were analyzed by bioinformatics and qRT-PCR. The transactivation activity and subcellular localization of OsDREB1J were analyzed by yeast heterologous expression, transient expression of rice protoplasts and tobacco. The overexpression and knockout mutant transgenic rice plants of OsDREB1J were obtained by genetic transformation system, and the grain size phenotypes were analyzed by phenotypic analysis technology. 【Result】Subcellular localization analysis showed that OsDREB1J was localized in the nucleus. Bioinformatics showed that the full-length coding sequence of OsDREB1J was 711 bp, encoding 236 amino acids. OsDREB1J protein had no transmembrane structure, and the molecular weight of 27.47 kDa, the theoretical isoelectric point of 5.54, and had a conserved AP2 domain unique to the AP2/ERF family. The cis-acting elements analysis of OsDREB1J promoter showed that the promoter contained cis-acting elements related to hormone response, light and stresses response. The qRT-PCR analysis showed that OsDREB1J was expressed in different tissues of rice with no tissue specificity, and the relative expression level in panicle was the highest. At the same time, OsDREB1J was induced or reduced by different hormone. Transcriptional activation analysis showed that the full-length of OsDREB1J has no transcriptional activity, but the C-terminal fragment was sufficient for the transactivation ability. Phenotypic analysis showed that the grain length, length-width ratio and thousand grain weight of osdreb1j mutant were significantly higher than those of ZH11, OsDREB1J overexpression transgenic rice plants displayed opposite phenotypes, while changing the expression of OsDREB1J did not affect rice grain width. These results show that OsDREB1J may affect grain size by regulating cell length rather than cell proliferation and cell expansion. 【Conclusion】In conclusion, OsDREB1J may be involved in regulating rice grain size through hormone signaling pathway.

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    Analysis of Genome-Wide Methylation Differences and Associated Gene Expression of Sesame Varieties Under High Temperature Stress
    SU XiaoYu, TAN ZhengWei, LI ChunMing, LI Lei, LU DanDan, YU YongLiang, DONG Wei, AN SuFang, YANG Qing, SUN Yao, XU LanJie, YANG HongQi, LIANG HuiZhen
    Scientia Agricultura Sinica    2024, 57 (24): 4825-4838.   DOI: 10.3864/j.issn.0578-1752.2024.24.001
    Abstract377)   HTML52)    PDF (3237KB)(238)       Save

    【Objective】This study aimed to explore the differences in genome-wide DNA methylation patterns and their relationships with associated gene expression in different heat-tolerant sesame varieties under high temperature stress, in order to gain a deeper understanding of the regulatory mechanisms of DNA methylation in sesame's response to high temperature stress, and to provide a theoretical basis for heat tolerance breeding in sesame. 【Method】Two sesame varieties, Zhengtaizhi 3 (heat-tolerant) and Shandong White Sesame (heat-sensitive), were selected as experimental materials and cultivated under high temperature (41 ℃) and control (30 ℃) conditions for 10 days. Nanopore sequencing technology was used to conduct methylation sequencing of the genomic DNA of these two sesame varieties, and transcriptome sequencing was performed to analyze changes in the expression of associated genes. Minimap 2 software was utilized for reference genome sequence alignment, and Tombo software was employed to detect 5mC, CpG, and 6mA methylation sites. Differentially methylated regions (DMRs) were identified based on a genome segmentation approach. Finally, functional annotation and pathway analysis of DMR-associated differentially expressed genes (DMR-DEGs) were conducted using GO, COG, and KEGG databases. 【Result】Under high temperature stress, significant changes were observed in the genome-wide DNA methylation patterns of both Zhengtaizhi 3 and Shandong White Sesame. Specifically, the m6A and cytosine methylation (mC) contents of Zhengtaizhi 3 increased, while those of Shandong White Sesame decreased. A total of 621 DMRs (Zhengtaizhi 3) and 374 DMRs (Shandong White Sesame) were identified across the entire genome, mainly distributed in promoter and intergenic regions. Further analysis revealed that these DMRs were significantly associated with 113 DMR-DEGs (Zhengtaizhi 3) and 56 DMR-DEGs (Shandong White Sesame), respectively, and that demethylated DMRs were closely related to upregulated gene expression. Functional annotation results indicated that these DMR-DEGs were primarily involved in biological processes such as carbohydrate transport and metabolism, posttranslational modification, protein turnover, signal transduction, and secondary metabolite biosynthesis. 【Conclusion】This study revealed the differences in genome-wide DNA methylation patterns and their relationships with associated gene expression in different heat-tolerant sesame varieties under high temperature stress. Zhengtaizhi 3, a heat-tolerant sesame variety, regulated the expression of related genes by increasing DNA methylation levels under high temperature stress, while Shandong White Sesame, a heat-sensitive variety, exhibited a decreasing trend in methylation levels. In particular, the dynamic changes in CpG site methylation played a crucial role in regulating sesame's response to high temperature stress. These findings provide new insights and theoretical support for understanding the mechanisms of sesame heat tolerance and for heat tolerance breeding.

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    Characterization and Correlation Analysis of Soil Dissolved Organic Matter and Microbial Communities Under Long-Term Application of Fresh and Composted Manure
    YUAN HuiLin, LI YaYing, GU WenJie, XU PeiZhi, LU YuSheng, SUN LiLi, ZHOU ChangMin, LI WanLing, QIU RongLiang
    Scientia Agricultura Sinica    2025, 58 (2): 307-325.   DOI: 10.3864/j.issn.0578-1752.2025.02.008
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    【Objective】This experiment was conducted to investigate the optical properties of dissolved organic matter (DOM) and the intrinsic relationship with soil microbial communities diversity and structure under long-term application of fresh and composted chicken/pig manure, so as to provide a theoretical reference of soil ecology for the implementation of recycling agriculture in the red soil area.【Method】Based on an 11-year (2011-2022) long-term experiment (sweet corn-sweet corn-cabbage rotation) of the National Soil Quality Guangzhou Red Soil Observatory Experiment Station, the fertilization regimes included no manure, chicken manure organic fertilizer, fresh chicken manure, fresh pig manure organic fertilizer, and fresh pig manure. Surface soil samples were collected and subjected to soil chemical properties determination, DOM UV-absorption characterization, parallel factor analysis of DOM fluorescence absorption characteristics, and Illumina MiSeq high-throughput sequencing, respectively. The main influencing factors were analyzed based on multivariate analysis.【Result】The composted manure significantly increased soil organic matter (122.5%-354.8%) and nutrient content, among which the chicken manure source treatments effectively increased soil available phosphorus content (1 697.2%-3 455.3%) and total phosphorus content (587.5%-812.5%), while swine manure source treatments mainly increased soil alkali-hydro nitrogen content (286.6%-311.3%) and total nitrogen content (326.4%-373.6%). Livestock manure applications, especially the composted manure, increased soil DOM content (60.3%-227.8%), among which the swine manure source treatments had a better effect on chromophoric dissolved organic matter content (118.1%-231.7%). In contrast, the chicken manure source treatments focused on increasing soil fluorescent dissolved organic matter (FDOM) content (293.4%-834.9%). For FDOM characteristic indexes, the biological index of manure application treatments was lower than that under CK (33.2%-39.2%), but the humification index was higher than that under CK (40.3%-43.3%). Four fluorescence components were identified with parallel factor analysis. The manure applications treatments mainly enriched the C3 (medium-size humus molecule containing fulvic acid and humic acid) and C4 (large-size humus molecule containing tryptophan) components, which promoted the conversion of protein-like components into humus-like components in FDOM. The maximum fluorescence intensities of the C3 and C4 components were higher in composted manure treatments. The composted chicken manure was more advantageous in increasing microbial community α-diversity, for the soil microbial community richness (Chao 1 index:19 065.6) and diversity (Shannon index: 5.6-6.0) were higher. The microbial community structures vary according to different treatments. The chicken manure source treatments were dominated by the eutrophic taxa Proteobacteria (31.2%-33.0%) and Gemmatimonadetes (4.1%), while the swine manure source treatments were dominated by the oligotrophic and efficient carbon-utilizing taxa Acidobacteria (21.0%-21.6%) and the nitrifying bacterial taxa Nitrospirae (2.6%-3.4%). Positive correlations dominated the co-occurrence networks, and Rhodobacteraceae had the highest number of correlations with other microbes. Redundancy Analysis and optimized random forest model showed that microbial communities were mainly influenced by available potassium and the C3 component of DOM, with a more pronounced response from nitrogen cycle-related microbial groups.【Conclusion】Long-term application of different manure sources mainly led to differences in humic components mediated by nutrients and organic matter input. Composted manure treatments improved the soil organic matter content and the degree of DOM humification. The available nutrients in soil and the humic-like components of DOM were the main factors affecting the structure of the soil microbial community. The response of nitrogen cycle-related microbial groups to these factors was particularly obvious and should be paid attention under long-term application of manure.

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    The Dilemma and Way Out of Patent Regulation for Gene-Edited Crops
    XU YiHeng
    Scientia Agricultura Sinica    2025, 58 (5): 831-839.   DOI: 10.3864/j.issn.0578-1752.2025.05.001
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    Gene-edited crops, the product of the intersection between biotechnology and agricultural science, represent a crucial direction in the development of modern agriculture. With the rapid advancement of the CRISPR-Cas9 system, the scientific research and commercial development of crop trait improvement have gradually shifted towards a “technology-driven” path, which has not only overturned traditional crop cultivation methods but also fundamentally propelled humanity’s exploration of crop research. Nevertheless, the phenomenon of patenting fundamental research tools has sparked widespread controversy within academia and profoundly impacted the sharing and utilization of crop resources. Private entities patenting CRISPR-Cas9 technology restrict other researchers and farmers’ opportunities to explore and harness genetic resources. This practice not only hinders scientific progress but also violates the fundamental consensus that genetic resources should be shared by all humanity. The sharing and openness of crop resources are crucial for the sustainable development of global agriculture and ecological balance, serving as a necessary condition for safeguarding public interests. A key issue that the governance of biotechnology patents urgently needs to address is how to reasonably allocate benefits and risks among traditional communities, researchers, research investors, and the public. This is also essential for constructing a new scientific ethics framework and regulating emerging technologies. However, China’s policy responses in this area are still insufficient. To mitigate the negative effects stemming from the exclusivity of patents, it is imperative to reassess and reconstruct the framework of relevant systems. Firstly, we should adhere to the principle of moral utility, emphasizing the public nature of scientific research and its social responsibilities, while carefully considering the “harmful” nature of inventions to social morality. Secondly, implementing a mandatory disclosure system for biological genetic resources is a crucial step towards achieving transparency and fairness, with “applicants truthfully disclosing the actual origin of crop genes based on the principle of good faith” elevated to a mandatory norm. Lastly, the open licensing of fundamental patented technologies can draw inspiration from the experience of open-source software, encouraging more researchers to participate in the exploration of crop resources through the open sharing of research tools, thereby facilitating broader scientific collaboration and the transformation of research outcomes.

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    Effects of One-Off Irrigation and Nitrogen Fertilizer Management on Grain Yield and Quality in Dryland Wheat
    WANG RongRong, XU NingLu, HUANG XiuLi, ZHAO KaiNan, HUANG Ming, WANG HeZheng, FU GuoZhan, WU JinZhi, LI YouJun
    Scientia Agricultura Sinica    2025, 58 (1): 43-57.   DOI: 10.3864/j.issn.0578-1752.2025.01.004
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    【Objective】The objective of this study was to evaluate the effects of one-off irrigation and nitrogen (N) fertilizer management on grain yield and quality in dryland winter wheat (hereinafter referred to as wheat).【Method】From 2020 to 2022, the split-plot field experiment was conducted at the Mengjin, Yichuan and Luoning counties of Luoyang city in Henan province, China, in the typical dryland wheat production area at the intersection between Loess Plateau and Huang-Huai-Hai Plain. The two irrigation levels of no-irrigation during wheat growth period (I0) and soil-moisture-based one-off irrigation after regreening (I1, one-off supplemental irrigation was conducted to 85% of field capacity when the soil water content in the 0-40 cm soil layer after wheat regreening was lower than 60% of field capacity at the first time, there was only this irrigation during the whole growth stage) were assigned as the main plots; and the four N fertilizer managements of N0, N120, N180 and N240 were set as sub-plots, where N rates were 0, 120, 180 and 240 kg·hm-2, respectively, and all of them were applied at sowing under I0 treatment, and 50% as basal, and 50% were applied along with the one-off irrigation under I1. The grain yield and protein yield, zinc content, protein and its components content, and main processing quality indicators of wheat were determined. 【Result】 Compared with I0, I1 significantly increased wheat grain yield and protein yield by 11.5%-73.0% and 9.1%-57.0%, respectively, in which the amplification enlarged with the increase of N rates, and that in the 2020-2021 was higher than that in the 2021-2022. However, I1 reduced the contents of zinc, and protein and its components in wheat grain, as well as the most indicators of processing quality compared with I0. Except for the globulin content at Yichuan in 2020-2021, compared with I0, I1 significantly decreased the content of zinc, protein and its components, and the processing quality of wheat grain. Among them, the content of zinc, albumin, globulin, gliadin, and glutenin were decreased by 5.0%-13.8%, 4.5%-14.1%, 6.4%-17.3%, 2.3%-24.8%, and 8.0%-13.9%, respectively, and the development time, stability time, wet gluten content, sedimentation value, extensibility, and maximum resistance were decreased by 5.3%-23.2%, 8.5%-51.1%, 2.0%-13.3%, 4.5%-18.1%, 4.6%-12.2% and 3.3%-10.6%, respectively. With the increase of N rate, wheat yield firstly increased and then decreased and wheat protein yield firstly increased and then stabilized under I0, while wheat yield firstly increased and then stabilized and wheat protein yield gradually increased under I1, however, the wheat quality indicators mostly tended to increase firstly and then stabilized under both I0 and I1. There were no significant differences between N240 and N180 for most quality indicators, but most of them were significantly higher than N120. The quality indicators of wheat grain under N120 were also significantly higher than that under N0. Considering the interaction effect, irrigation levels and N managements had significant interaction effects on yield and protein yield, but there was no interaction effect on most quality indicators. The grain yield under I1N180 did not change significantly but the protein yield was significantly decreased by 3.9%-4.9% compared with I1N240, however, both the grain yield and protein yield under I1N180 were significantly increased compared with other treatments. Although the content of protein and protein components and the processing quality in wheat grain under I1N180 were significantly lower than that under I0N180 and I0N240, these quality indicators were not significantly lower than that under I1N240. 【Conclusion】 The soil-moisture-based one-off irrigation after regreening combined with the N rate of 180 kg·hm-2 and 50% N topdressing along with irrigation (I1N180) could synchronously improve the grain yield and stabilize the grain quality in dryland wheat. Therefore, I1N180 was suitable for wheat production in the dryland area, where one-off irrigation was guaranteed due to High-Standard Farmland Construction Program.

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    Heterosis Groups Research in Maize Inbred Lines Based on Machine Learning
    CAO ShiLiang, ZHANG JianGuo, YU Tao, YANG GengBin, LI WenYue, MA XueNa, SUN YanJie, HAN WeiBo, TANG Gui, SHAN DaPeng
    Scientia Agricultura Sinica    2025, 58 (2): 203-213.   DOI: 10.3864/j.issn.0578-1752.2025.02.001
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    【Objective】The objective of this study is to optimize the classification and discriminant method of maize heterotic groups, and provide guidance and reference for maize breeding practices.【Method】Solid-phase chips were used to genotype 60 waxy maize inbred lines, and high-quality SNP markers with different density were obtained through quality control. Population structure analysis and genetic distance clustering were used to classify the 60 waxy maize inbred lines into different groups, and the differences between different classification methods were compared. On this basis, random forest and support vector machine methods were used to sample and discriminate the results of different classification methods. Five-fold cross-validation was used for sampling, and the prediction accuracy of maize group classification based on different classification methods was compared.【Result】Using different quality control standards, 11 431 and 4 022 molecular markers were obtained, respectively. Based on these two molecular marker densities, 60 materials were divided into 5 and 4 clusters, respectively. When using 11 431 SNP markers, the population structure analysis and genetic distance clustering results showed that the intra-cluster sample consistency was 63.33%. When using 4 022 SNP markers for clustering, the intra-cluster sample consistency was 90.00%. The prediction accuracy results for discriminating maize inbred line clusters showed that the average prediction accuracy (91.43%) of Random Forest and Support Vector Machine using 4 022 markers were higher than that of 11 431 markers (86.25%). Among them, the highest prediction accuracy was achieved by Random Forest using 4 022 markers, with a prediction accuracy of 94.17%.【Conclusion】Clustering analysis ultimately divided 60 waxy maize inbred lines into 4 clusters. Sampling and cross-validation results using Random Forest and Support Vector Machine for cluster classification showed that Random Forest achieved higher prediction accuracy than Support Vector Machine.

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    Influence of Expressing OsNRAMP5 Under the Driving of the OsLCT1 Promoter on Cadmium Migration to Rice Seeds
    XIONG JiaNi, LI ZongYue, HU HengLiang, GU TianYu, GAO Yan, PENG JiaShi
    Scientia Agricultura Sinica    2025, 58 (7): 1259-1268.   DOI: 10.3864/j.issn.0578-1752.2025.07.001
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    【Objective】Cadmium (Cd) is the predominant pollutant in China’s arable land, with rice cultivated on these contaminated soils being a significant dietary source of Cd for the population. This study aims to tissue-specifically express OsNRAMP5, a transporter responsible for the majority of Cd uptake in rice, to investigate strategies for developing low-Cd rice varieties and provide a reference for molecular design breeding to cope with Cd pollution. 【Method】To drive the expression of OsNRAMP5 in rice, we utilized a 2 500 bp sequence upstream of the OsLCT1 start codon as the promoter. The red fluorescent protein mRFP was fused to the C-terminus of OsNRAMP5 to visualize its tissue localization. After obtaining independent homozygous transgenic lines, the transcripts of the OsNRAMP5 were first detected using qRT-PCR, and its tissue localization in roots and nodes was observed via laser confocal microscopy. Subsequently, the accumulation and tolerance of Cd were evaluated in transgenic and wild-type rice under varying concentrations of Cd treatment. Furthermore, plants were grown in Cd-contaminated paddy soil, and the accumulation of Cd and other mineral elements in seeds and leaves, as well as related yield traits, were measured. 【Result】Under the drive of the OsLCT1 promoter, OsNRAMP5 was expressed mainly in the epidermis, exodermis and stele of roots, as well as in the phloem area of enlarged vascular bundles and diffuse vascular bundles in nodes, differing significantly from the native expression pattern of OsNRAMP5 in rice. Compared to wild-type rice, the transgenic lines exhibited increased Cd accumulation in roots, decreased Cd accumulation in shoots, and enhanced tolerance to Cd stress during the seedling stage. When cultivated in Cd-contaminated paddy soils, plant height and grain yield were unaffected by the ectopic expression of OsNRAMP5, while Cd accumulation in seeds and leaves significantly decreased in the transgenic lines. The Cd content in seeds decreased by over 80%, with a greater reduction ratio compared to that in leaves. Although the Mn content in seeds and leaves slightly decreased, the expression of OsNRAMP5 had little impact on the accumulation of other mineral elements such as Fe, Zn, and Cu. 【Conclusion】The expression of OsNRAMP5 driven by the OsLCT1 promoter greatly decreases the Cd migration toward rice seeds by reducing Cd transport to the aboveground parts from roots and increasing the Cd transporting to leaves at nodes. Therefore, the expression of OsNRAMP5 under the control of the OsLCT1 promoter is an effective strategy to reduce Cd accumulation in rice seeds.

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    Physiological Characteristics in Response to Salt Stress and Allelic Variation and Expression of Salt-Responsive Genes in Seedling Stage of Nangeng Rice Varieties with Salt-Tolerance Ability
    DENG LiCheng, LI Cheng, HE Lei, AN HongQiang, WANG CaiLin, ZHANG YaDong, ZHAO ChangJiang, LU Kai
    Scientia Agricultura Sinica    2025, 58 (12): 2275-2290.   DOI: 10.3864/j.issn.0578-1752.2025.12.001
    Abstract358)   HTML53)    PDF (2427KB)(219)       Save

    【Objective】Salt stress is one of the main environmental stresses that restrict rice production. Studying the physiological characteristics under salt stress and analysis the allelic variation and expression of salt-tolerance genes provide key gene resources and genetic materials for breeding salt-tolerance rice varieties. 【Method】This study first evaluated the salt-tolerance ability of the Nangeng series high-quality rice varieties/lines during the seedling stage, using survival rate as an indicator for screening salt-tolerance varieties, which physiological changes under salt stress were analyzed, including chlorophyll, Na+, K+, MDA, H2O2 and soluble sugar. The variation types and expression levels of salt-tolerance genes in rice varieties with resistance to high salt concentration were also analyzed to explaining their molecular mechanisms in response to salt stress. 【Result】Under the condition of treating with 140 mmol·L-1 NaCl for 6 days, the survival rates of NG9108, NG5718, and NGY1 were greater than 60%, with the highest survival rate among the tested varieties. Compared with Nipponbare, the seedlings of NG9108, NG5718, and NGY1 under salt stress had higher chlorophyll content and lower MDA content, indicating that salt stress caused less cell damage to the three varieties. The Na+/K+ values in the roots of NG9108, NG5718, and NGY1 were significantly higher than those in Nipponbare, while the Na+/K+ values in the aerial parts were significantly lower than those in Nipponbare, implying that the three varieties absorb or store more Na+ in roots, but transport less Na+ upwards, which is beneficial for maintaining cell ion balance and causing less ion toxicity and osmotic stress in aerial parts of the seedlings. The three salt-tolerance varieties have 94 SNPs or InDel sites, distributing in exons, introns, 5′UTR, and 3′UTR of the 23 salt-tolerance genes. 24 variation sites of 11 genes occur in the exons, including 7 genes with frameshift mutations or missense mutations which distributed in Os02g0813500 (OsGR2), Os05g0343400 (OsWRKY53), Os06g0685700 (OsRST1), Os07g0685700 (OsEIL2), Os10g0431000 (OsPQT3), Os11g044600 (OsRSS3), Os12g0150200 (P450). Salt stress significantly induces expression of OsSKC1, OsBAG4, OsGPX1, OsCCX2, OsGR3, OsDREB2a, OsRAB21, OsP5CS, OsbZIP23, OsAPX37 and OsLEA3, which help to enhance salt tolerance and reduce the adverse effects of salt damage on rice growth. 【Conclusion】NG9108, NG5718 and NGY1 showed strong salt tolerance phenotype during the seedling growth stage, which is closely related to the balance of sodium and potassium ions under salt stress, allelic variations of multiple salt tolerance genes, and gene expression levels. NG9108, NG5718 and NGY1 have pyramided multiple salt tolerant and high-quality genes, which can be used as backbone parents for genetic improvement and breeding.

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    Screening and Identification of Drought-Tolerant Sweet Potato Germplasm Resources
    CHEN YongXian, CHEN RuiJiang, DU YiZhi, ZHU JunJie, CHEN WanXia, ZHAO ZiHan, WANG JiChun, DU Kang, ZHANG Kai
    Scientia Agricultura Sinica    2025, 58 (2): 214-237.   DOI: 10.3864/j.issn.0578-1752.2025.02.002
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    【Objective】Seeking key indicators and methods for accurately characterize drought tolerance in sweet potato, and screening and identifying drought-tolerant sweet potato germplasm resources, to provide effective methods for the rapid and accurate identification of drought-tolerant sweet potato germplasm resources, and to provide material and theoretical basis for selection and breeding of high quality and drought-tolerant sweet potato varieties. 【Method】Fifty-four sweet potato germplasm resources were used as materials for drought stress experiments. By using two treatments including drought stress and control, and combining with drought pool cultivation experiment and field test, the effects of drought stress on the growth and development, physiological and biochemical characteristics, antioxidant metabolism, photosynthetic characteristics and yield of different sweet potato germplasm resources were investigated, the response characteristics of different sweet potato germplasm resources to drought were analyzed, and the effective indicators for drought tolerance evaluation in sweet potato were selected. The drought tolerance evaluation was preformed using principal component analysis, correlation analysis, direct evaluation of drought resistance coefficient, and calculation of comprehensive drought tolerance measurement value (D value) based on membership function, and the drought-tolerant sweet potato germplasm resources were screened and identified.【Result】The results obtained from the drought pool cultivation experiment showed the influences of drought treatment on the main stem length, aboveground fresh weight, underground dry weight and fresh weight of storage root were extremely significant (P<0.01), and eight drought-tolerant germplasm resources were screened based on cluster analysis of D values. In the field test, the main stem length, stem diameter, number of branches, leaf area index, leaf relative water content, total chlorophyll content, stomatal conductance, net photosynthetic rate, transpiration rate, intercellular carbon dioxide concentration, proline (Pro), malondialdehyde (MAD), peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) showed highly significant differences (P<0.01) under drought stress when compared with control. Through the establishment of regression models, it could be initially determined that eight indicators including the leaf area index, root tip, leaf POD, leaf APX, storage root Pro, storage root SOD, storage root CAT, and yield could be used as indicators for drought tolerance identification in sweet potato. XN18111-1, 20XN18-1, XN1834-11 and XN17104-46 were classified as drought-tolerant germplasm resources according to grading of drought resistance coefficient based on yield. The D values of XN18111-1, 20XN18-1 and XN1862-61 were over 0.6 and showed high drought tolerance based on comprehensive drought tolerance evaluation. 【Conclusion】Based on results of comprehensive drought tolerance evaluation in drought pool cultivation experiment, as well as the comprehensive drought tolerance evaluation and yield evaluation in field test, XN18111-1 and 20XN18-1 were finally identified as drought-tolerant germplasm resources, which can be used as drought-tolerant breeding materials or ideal resource materials for study on drought-tolerance mechanism in sweet potato.

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    Identification of Alfalfa (Medicago sativa) MsCEP Genes and Functional Analysis of Its Regulation in Root Growth and Development
    ZENG XiangCui, YANG YongNian, LI RuYue, JIANG XueQian, JIANG Xu, XU YanRan, LIU ZhongKuan, LONG RuiCai, KANG JunMei, YANG QingChuan, LI MingNa
    Scientia Agricultura Sinica    2024, 57 (24): 4839-4853.   DOI: 10.3864/j.issn.0578-1752.2024.24.002
    Abstract351)   HTML50)    PDF (3483KB)(190)       Save

    【Objective】CEP (C-terminal encoded peptides) is a gene that encodes hormone-like peptides secreted by roots and serves as a key regulator of plant root growth and development. To provide a molecular theoretical basis for further elucidating the function of MsCEP genes in root growth and development, members of the Medicago sativa MsCEP gene family, basic characteristics, expression differences in different tissues, and their roles in root growth were identified and analyzed. 【Method】Based on the genomic information of the alfalfa cultivar Xinjiang Daye, the MsCEP gene family members of alfalfa were accurately identified using local Blast analysis in TBtools and feature domain by referring to MtCEP family protein of Medicago truncatula sequence. The fundamental genetic and protein characteristics and the phylogenetic relationship of the MsCEP genes were analyzed by bioinformatics methods. The expression patterns of alfalfa MsCEP gene family members in various tissues were assessed using transcriptome data and real-time fluorescence quantitative PCR. The functional roles of mature MsCEP peptides in root growth and development were analyzed by exogenous application experiments. 【Result】A total of 35 MsCEP family members were identified in the genome of alfalfa Xinjiang Daye, and these genes are distributed across 18 chromosomes, lack introns, and all possess an N-terminal signal peptide and one or two conserved domains of the CEP family. The MsCEP members displayed predicted amino acid length ranging from 59 to 150, with molecular weights spanning 6.7 to 16.2 kDa, the isoelectric points varying from 5.80 to 10.41, instability indices ranging from 30.63 to 89.93, aliphatic indices ranging from 54.41 to 134.88, and the grand average of hydropathicity ranging from -1.110 to 0.377. Subcellular localization predictions indicated that the MsCEP protein predominantly localizes to the nucleus, plasma membrane, chloroplast, and Golgi apparatus. Cluster analysis delineated three distinct branches within the family, aligning with counterparts from Arabidopsis thaliana and Medicago truncatula. The largest branch encompassed 48 CEP members. Collinearity analysis highlighted a collinear relationship between the MsCEP genes in alfalfa and those in Arabidopsis thaliana and Medicago truncatula. Tissue expression analysis revealed that members of the MsCEP family exhibit distinct tissue-specific expression patterns, with higher expression levels in roots and lower or no expression detected in leaves. Among them, 22 members exhibited higher expression levels in roots compared to other tissues. The exogenous application of synthetic mature MsCEP2 peptide suppressed the growth of primary and lateral roots, reduced the number of lateral roots, and decreased the density of lateral roots. 【Conclusion】In conclusion, our investigation identified a total of 35 MsCEP members from the alfalfa 'Xinjiangdaye' genome database, which are revealed to be highly conserved. The MsCEP genes are primarily expressed in roots, and the exogenous application of synthetic mature MsCEP peptides can regulate root morphology, indicating that MsCEP peptides play important roles in root growth and development of alfalfa.

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    Research Progress on Mechanisms Interpretation and Prediction Methods for Heterosis of Livestock
    SUN YanYan, NI AiXin, YANG HanHan, YUAN JingWei, CHEN JiLan
    Scientia Agricultura Sinica    2025, 58 (5): 1017-1031.   DOI: 10.3864/j.issn.0578-1752.2025.05.015
    Abstract336)   HTML33)    PDF (1252KB)(777)       Save

    Heterosis is a phenomenon where the offspring of genetically distinct populations exhibit superior vitality, reproductive capacity, and adaptability compared with the average of their parent populations, which is an important genetic resource. Heterosis plays a significant role in modern agriculture, contributing to increase yields and quality of livestock and crops, rapidly improve traits, accelerate the breeding of new varieties, and enhance genetic diversity, thereby efficiently boosting the production of animal husbandry and agriculture while reducing costs. Despite the discovery of heterosis is over a century ago, the elucidation of its genetic basis lags far behind its application in agricultural production. The study of the complex formation mechanism of heterosis is a classic and an active topic in the field of genetics and breeding, but the clear conclusions remain limited. In response to the characteristics of heterosis, scientists have successively proposed various hypotheses for its formation, such as the dominance hypothesis, overdominance hypothesis, and epistasis hypothesis, revealing that the genetic basis of heterosis was non-additive genetic effects. However, these hypotheses are based on the effects of single genes, which are overly idealized and simplistic. Explorations at different levels, such as DNA, RNA, and proteins, have successively discovered the coexistence of multiple genetic effects. Particularly in hybrid crops like rice and corn, the related researches have been continuously identified the loci of heterosis effects, enriched the understanding of the formation mechanism for heterosis in crops, and promoted the transformation of crop breeding technologies, such as precise molecular design breeding. Heterosis is also widely applied in the breeding of livestock and poultry. In developed countries with advanced animal husbandry, over 80% of commercial pork, chicken, and eggs are obtained from hybrid breeds. To efficiently apply heterosis in production for animal husbandry, it is necessary to predict heterosis in advance. New methods, such as the inter- and intra-group phenotypic variance ratio prediction, hybrid heritability prediction, and molecular marker prediction, have been developed to solve the long experimental cycle, environmental sensitivity, and high human and financial costs associated with traditional hybridization experiments for predicting heterosis. However, the accuracy of these prediction methods is limited. Heterosis involves in interaction of multiple levels, and because of the complex genetic background and long breeding cycle, it is still a big challenge for the study of the heterosis formation mechanism and accurate prediction methods. In recent years, the gradual application of sequencing technology has provided a new perspective for understanding the molecular regulatory network of heterosis in livestock and poultry. QTL mapping and genome-wide association study reveal the molecular mechanism of heterosis at the genomic level, and the identified molecular makers are applied in selection and breeding. Combined with multi-omics researches, such as transcriptomics and metabolomics, the key functional genes, variations, and metabolites affecting heterosis can be more precisely located, which facilitate hybrid improvement. This review elaborated the research progress in the formation mechanism and prediction methods for heterosis in the field of livestock and poultry. For looking forward to future, the researches will gradually clarify the complex mechanism of heterosis by integrating multi-omics sequencing data and bioinformatics analysis, in order to identify genes and molecular markers related to heterosis, and innovate new prediction methods, which will provide a more accurate direction for the utilization of heterosis.

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    Screening and Identification of Pigm-1 Interaction Proteins for Disease Resistance of Rice Blast
    JIN YiDan, HE NiQing, CHENG ZhaoPing, LIN ShaoJun, HUANG FengHuang, BAI KangCheng, ZHANG Tao, WANG WenXiao, YU MinXiang, YANG DeWei
    Scientia Agricultura Sinica    2025, 58 (6): 1043-1051.   DOI: 10.3864/j.issn.0578-1752.2025.06.001
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    【Objective】 Rice blast is one of the most devastating diseases of rice production. A broad-spectrum disease resistance gene Pigm-1 was identified but its functional pathway and interactors are unknown. The screening and identification of key proteins in the Pigm-1 signaling pathway will provide an important theoretical basis for rice disease resistance breeding. 【Method】 In this study, the decoy protein pGBKT7-Pigm-1-CC1-576 vector was constructed to detect the decoy protein self-activation, and the toxicity of the decoy protein was detected by separately transforming the plasmid pGBKT7 and pGBKT7-Pigm-1-CC1-576 into Y2H Gold yeast. The rice disease resistance R protein Pigm-1 was screened by cDNA expression yeast library induced by rice blast fungus. The sequencing results were compared and annotated by Rice Information GateWay (RIGW). The interaction of OsbHLH148 protein was verified by Luc, Co-IP and yeast two-hybrid assays, and the tissue expression of the corresponding gene of the interaction protein OsbHLH148 was analyzed by qRT-PCR. 【Result】 The self-activation test showed that the decoy protein pGBKT7-Pigm-1-CC1-576 did not self-activate when cotransformed with the AD plasmid, and the toxicity analysis showed that the decoy protein had little or no toxicity to yeast cells. A total of 124 proteins that may interact with Pigm-1 were obtained by screening the yeast library, and among these proteins, there are ethylene synthesis related, gibberellin synthesis related, active oxygen species clearly related, enzyme metabolism related, and some function unknown. The interaction between Pigm-1-CC1-576 and OsbHLH148 was verified by Luc, Co-IP and yeast two-hybrid methods. Further analysis showed that OsbHLH148 can be induced by blast fungus infection, and the tissue expression analysis showed that OsbHLH148 expression level was the highest in rice leaves at 6 weeks. 【Conclusion】 In this study, 124 proteins that may interact with Pigm-1 were obtained. One of these proteins, OsbHLH148, was selected and verified to interact with Pigm-1-CC1-576. Suggesting that OsbHLH148 may be involved in Pigm-1 mediated resistance of rice blast.

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    Study on the Involvement of OsFWL3 in the Regulation of Metal Ion Transport and Accumulation in Rice
    LIU YiHan, MU QingShan, HE Xiang, CHEN Min, HU Jin, GUAN YaJing
    Scientia Agricultura Sinica    2024, 57 (21): 4161-4174.   DOI: 10.3864/j.issn.0578-1752.2024.21.001
    Abstract335)   HTML57)    PDF (5657KB)(239)       Save

    【Objective】FWL (Fruit Weight2.2-Like) gene is a negative regulator of cell proliferation, which not only regulates plant organogenesis and organ size, but also participates in the regulation of metal ion transport accumulation and signal transduction. Analyzing of the function of OsFWL3 gene is helpful to reveal the transport mechanism of trace metal elements in crops. It provides theoretical support for reducing heavy metal accumulation and improving crop quality. 【Method】The gene information, genome structure and phylogenetic tree of OsFWLs family were analyzed by bioinformatics method, and the expression profile of OsFWL3 gene was predicted. Two OsFWL3 knockout lines were obtained using CRISPR/Cas9 gene editing technology. Then wild type and Osfwl3 mutants were treated with ZnSO4 at seedling stage and filling stage, respectively. The phenotypes of plants and grains after treatment were analyzed, and the content variation of metal elements such as Zn was determined to explore the effects of OsFWL3 on the transport and accumulation of metal ions and seed quality. 【Result】The gene function of OsFWLs family is similar to some extent. OsFWL3 gene is highly expressed in anther and panicle, indicating that it is closely related to reproductive development of rice. The number of primary branches, grian length, grain thickness and 100-grain weight of Osfwl3 mutants are significantly larger than WT. OsFWL3 affects the content and distribution of Zn and other metal ions in rice seedlings and grains. The deletion of OsFWL3 gene affects the competitive transport of Zn, Cd and Mn from underground to above-ground, lower grain to central grain and husk to brown rice. 【Conclusion】OsFWL3 gene affects the distribution of Zn and other metal ions in rice grains and plants, and it plays an important role in regulating the growth and development of rice plants and grain size.

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    Research Progress and Prospects on Crop Pan-Genomics
    WANG Hui, DING BaoPeng, LI YuXian, REN QuanRu, ZHOU Hai, ZHAO JunLiang, HU HaiFei
    Scientia Agricultura Sinica    2025, 58 (11): 2045-2061.   DOI: 10.3864/j.issn.0578-1752.2025.11.001
    Abstract331)   HTML40)    PDF (1774KB)(212)       Save

    The global population continues to rise and climate change imposes severe challenges on food supply, the issue of food security has become increasingly prominent. To meet the growing demand for food, enhancing crop yield and improving environmental adaptability have become critical goals in agriculture. Under this situation, genomics is regarded as an essential method for accelerating crop breeding, as it enables the in-depth exploration and utilization of superior functional genes to not only boost crop productivity but also strengthen stress tolerance and adaptability, thereby providing robust support for ensuring global food security and achieving sustainable agricultural development. Nonetheless, the traditional single-reference genome often fails to capture the entire spectrum of genomic variations accumulated during crop domestication and improvement, which constrains our understanding of functional genes and their regulatory networks. With the continual advancement of high-throughput sequencing technologies, genomics research has now entered the pangenomics era. By integrating multiple high-quality genomes into a comprehensive catalog of genomic content, researchers can precisely identify a variety of genetic variations, including single nucleotide polymorphisms (SNPs) and structural variations (SVs), thereby capturing the extensive genetic diversity present across different cultivars, subspecies, and wild relatives. Pangenomics framework greatly facilitates the exploration of superior functional genes. Moreover, by combining pangenomic data with other multi-omics datasets (e.g., transcriptomics, proteomics, and epigenomics), researchers can accurately identify superior functional genes, enabling the provision of more targeted and accurate genetic loci for molecular breeding. With emerging gene-editing tools such as CRISPR-Cas9, researchers can further modify essential genetic loci in a directed manner to remove undesirable traits or reinforce resistance to environmental stressors. This will lay a foundation for cultivating the next generation of crops that exhibit higher yield, improved quality, and enhanced resilience. This review summarizes recent developments in major pangenome construction methods and formats, and systematically reviews the progress made in crop pangenomes as well as their applications in crop breeding improvement. It also discusses the challenges pangenomics faces in future crop breeding, offering insights into leveraging pangenome resources for crop genetic improvement, and ultimately provides new perspectives and strategies for future molecular breeding.

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    Characteristics of phoD-Harboring Microbial Communities Under Long-Term Fertilization and Its Effects on Organic Phosphorus Fractions in Black Soil
    SHEN WenYan, ZHANG NaiYu, LI TianJiao, SONG TianHao, ZHANG XiuZhi, PENG Chang, LIU HongFang, ZHANG ShuXiang, DUAN BiHua
    Scientia Agricultura Sinica    2024, 57 (20): 4082-4093.   DOI: 10.3864/j.issn.0578-1752.2024.20.013
    Abstract329)   HTML34)    PDF (1359KB)(143)       Save

    【Objective】The effects of long-term fertilization on the organic phosphorus (Po) fractions and phoD-harboring microbial community characteristics in black soil were analyzed, as well as the response of Po fractions to key microbial community characteristics was explored, so as to provide a theoretical basis for the efficient utilization of phosphorus in black soil and for the establishment of scientific fertilization strategies.【Method】Based on the long-term fertilization experiment started in 1989 in black soil, five fertilization treatments were selected: no-fertilizer (CK), nitrogen and potassium fertilizers (NK), nitrogen, phosphorus and potassium fertilizers (NPK), nitrogen, phosphorus and potassium fertilizers plus straw return (NPKS), and nitrogen and phosphorus and potassium fertilizers plus manure (NPKM). Soil samples collected from the 0-20 cm in 2018 were used for the study. Chemical sequential fractionation was performed to quantitatively analyze the Po fractions. Illumina Miseq high-throughput sequencing platform and real-time PCR technology were used to qualitatively and quantitatively analyze the characteristics of phoD-harboring microbial community in soil. The relationships among phoD-harboring microbial community composition, Po fractions and soil properties were comprehensively explored through correlation and variance partitioning analysis.【Result】(1) Except for NPKM treatment, there was no significant difference in total Po content among different fertilization treatments, while there was a significant difference in the content and proportion in the total Po of Po fractions. Compared with CK, the content of labile Po (LOP) and moderately labile Po (MLOP) were significantly increased under NK and NPKM treatment, and the content of LOP and MLOP under NK increased by 108.7% and 27.5%, respectively, whereas that under NPKM treatment increased by 446.6% and 38.1%, respectively. Compared with the NPK treatment, the content of LOP and MLOP under the NPKS treatment were significantly reduced by 57.7% and 24.0%, respectively. (2) The fertilizer application with organic materials (NPKS and NPKM) changed the community composition of phoD-haboring microorganisms, but had no effect on their diversities. The NPKS treatment significantly increased the abundance of Pseudomonas, and the NPKM treatment significantly increased the abundance of Gemmatimonas. Meanwhile, the NPKS treatment also significantly increased the absolute abundance of phoD genes and alkaline phosphatase (ALP) activity. (3) Correlation analysis showed that there was a significant relationship among phoD-harboring microbial community composition, LOP and MLOP. Variance partitioning analysis showed that phoD-harboring microbial community composition individually explained 12.1% and 10.2% of the variations in the content and proportion of Po fractions, whereas that were 58.5% and 58.7% jointly with ALP activity, and 67.5% and 64.7% jointly with soil organic matter (SOC), respectively. It could be seen that community composition and soil properties (ALP activity, SOC) jointly affected organic phosphorus components, and their impact effect was better than individual indicators.【Conclusion】Long-term fertilization applications altered phoD-harboring microbial community composition, which interacted with ALP activity and SOC explained changes in Po fractions. Chemical fertilizer combined with straw could improve phoD-harboring microbial community characteristics and significantly increased alkaline phosphatase activity, thereby promoting Po mineralization and improving the utilization efficiency of phosphorus in black soil.

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    Effects of Nitrogen Fertilization Levels on Matter Accumulation and Nitrogen Uptake in Different Source and Library Types of Japonica Rice
    ZHOU Yu, SUN Tong, ZHANG YanHong, RU Yan, SU Tan, WANG Shuai, ZHU JinYan, HU JinLong, XIONG QiangQiang, ZHANG HongCheng, ZHOU NianBing
    Scientia Agricultura Sinica    2025, 58 (11): 2096-2117.   DOI: 10.3864/j.issn.0578-1752.2025.11.004
    Abstract323)   HTML37)    PDF (779KB)(178)       Save

    【Objective】 This study aimed to investigate the overground part material accumulation and nitrogen transport of japonica rice with different sources and library types under different nitrogen application conditions, in order to select the japonica rice strain with high yield and high nitrogen efficiency, and explore the optimal nitrogen application rate for the growth of this japonica rice variety, ultimately achieve an increase in yield.【Method】 The trial was conducted in 2022-2023, with the source library interaction type Yangchan35003, source restriction type Yangchan35002, and library restriction type Yangchan35004 as test materials, and the split zone design was adopted. Four nitrogen application treatments of 180 kg·hm-2, 225 kg·hm-2, 270 kg·hm-2 and 315 kg·hm-2 were set. The yield, dry matter accumulation and nitrogen related indexes (mainly taking nitrogen transport efficiency, nitrogen transport contribution rate, harvest index, nitrogen harvest index, grain production efficiency, dry matter production efficiency and nitrogen fertilizer productivity as the evaluation indexes of nitrogen utilization efficiency) of different types of japonica rice under each treatment were measured at the key stage, the changes of different types of japonica rice under different nitrogen treatment were analyzed in the bar chart, and the influence of nitrogen application on material accumulation and transport was delved; the index mean value and variation coefficient of japonica rice were compared, the differences in indicators caused by different nitrogen application amounts were eliminated, and the differences between different types of japonica rice were highlighted; by using the correlation coefficient, the relationship between yield types of japonica rice and nitrogen utilization efficiency were explored, and the influence of nitrogen application on the accumulation of dry matter, nitrogen utilization and yield of japonica rice in heading and maturity period were analyzed.【Result】 (1) Applying additional nitrogen fertilizer within the range of 180-315 kg·hm-2 could significantly increased the yield of each type of japonica rice (9.68%-29.19%), dry matter and nitrogen accumulation at heading stage (3.26%-28.57% and 8.57%-47.91%), and dry matter and nitrogen accumulation at maturity stage (1.01%-24.84% and 3.83%-57.98%) during the two-year period. Meanwhile, it was also beneficial to improve nitrogen transport capacity of leaves and stem at maturity stage. (2) Under the same nitrogen application conditions, the accumulation of dry matter, the accumulation of nitrogen, 1000-grain weight and setting percentage in Yangchan35003 were higher than that in Yangchan35002 and Yangchan35004 (29.08%-44.12%, 9.32%-29.21%, 11.40%-15.42%, 17.67%-40.70% and 25.93%-44.13%, 18.25%-26.17%, 6.16%-8.30%,-1.50%-13.96%, respectively) during the two-year period, the final yield exceeded the two types of japonica rice (24.26%-32.33% and 21.14%-30.05% higher than the yield Yangchan35002 and 35004, respectively) during the two-year period.(3) The high nitrogen transport capacity was not a unique feature of the source library interaction type strain. In this experiment, under the same nitrogen level condition, the nitrogen transport efficiency of Yangchan35004 was similar to the source library interaction type strain in the experiment, but the nitrogen intake was lower than Yangchan35003 (15.43%-20.74%), and the yield was lower than Yangchan35003 (17.45%-23.11%) during the two-year period, so it was a low yield and high nitrogen efficiency rice strain.【Conclusion】 Under the condition of applying 270 kg·hm-2 of nitrogen fertilizer, Yangchan35002 could significantly optimize the synergy utilization efficiency of nutrients and temperature-light resources, enhance the nitrogen absorption and transportation capacity of plants, and improve the yield performance of the population. It was determined as the optimal combination in this experiment.

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    Effects of Planting Density on the Pod Characteristics and Exploring Strategie Analysis to Increase Yield in High-Yield Rapeseed
    LI YiYang, WANG Long, QIAN Chen, LI Jing, LIN GuoBing, QU WenTing, WANG Yan, LIN YaoWei, HUANG YiHang, ZHENG JingDong, YOU JingJing, ZUO QingSong
    Scientia Agricultura Sinica    2024, 57 (22): 4459-4472.   DOI: 10.3864/j.issn.0578-1752.2024.22.006
    Abstract321)   HTML82)    PDF (520KB)(325)       Save

    【Objective】Planting density is an important factor affecting pod quantity and quality in rapeseed. Based on a certain number of rapeseed pod quantity, the quality of rapeseed kernels was improved to further tap the potential of rapeseed production, so as to explore the effect of kernels quality on the formation of rapeseed population yield.【Method】The experiment was conducted to investigate the effects of planting density on rapeseed yield, canopy, and pod characteristics during the 2021 to 2022 and 2022 to 2023 growing seasons in Shiye Experimental site of Yangzhou University in Zhenjiang. Qinyou10 and Ningza1838 were used as the rape test materials. Five planting density levels were set as 2.4×105 plant/hm2 (D1), 3.6×105 plant/hm2 (D2), 4.8×105 plant/hm2 (D3), 6.0×105 plant/hm2 (D4), and 7.2×105·plant/hm2 (D5). 【Result】As planting density increased from D1 to D5, the number of pods in population and plot seed yield initially increased and decreased thereafter. The number of pods in population in D3 and D4 was higher than that in other densities, and plot seed yield reached the maximum value in D2 and D3. The canopy thickness, ranging from 41.50 cm to 80.98 cm across different treatments, increased with an increase in planting density from D2 to D5. The pod density, ranging from 0.98×104 to 2.16×104 per cubic meter, significantly increased as planting density increased. According to the number of seeds per pod across different treatments, the pods were categorized into low efficient pod (≤14), middle efficient pod (15 to 17), and high efficient pod (≥18), respectively. The average yield per pod of low, middle, and high efficient pod was 19.40×10-3, 53.41×10-3 and 80.62×10-3 g, respectively. The allocation ratio of pod number ranged from 27.60% to 40.96% for low efficient pod, 8.39% to19.73% for middle efficient pod and 39.31% to 63.28% for highly efficient pod, respectively. Among the three types of pods, the ratio of yield for highly efficient pod was the highest and the range of variation for low, middle and high efficient type was from 8.41% to 15.62%, 7.24% to 22.01% and 62.60% to 83.92%, respectively. As planting density increased from D3 to D5, the quantity and allocation ratio of highly efficient pod significantly decreased, resulting in decreasing seed yield. 【Conclusion】When the target seed yield was 4 500 kg·hm-2, the appropriate planting density and population quality indicators with suitable sowing date were as follows: planting density ranged from 3.6 to 4.8×105 plant·hm-2, the number of pods in population ranged from 83.0 to 94.0×106·hm-2, and the allocation ratio of high efficient pod (≥18 seeds per pod) is more than 50%. Increasing the number of seeds per pod to improve the quantity and allocation ratio of highly efficient pod was an effective way to further enhance the seed yield in rapeseed.

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    Genome-Wide Association Study of Heat Tolerance at Seedling Stage in A Wheat Natural Population
    LI YunLi, DIAO DengChao, LIU YaRui, SUN YuChen, MENG XiangYu, WU ChenFang, WANG Yu, WU JianHui, LI ChunLian, ZENG QingDong, HAN DeJun, ZHENG WeiJun
    Scientia Agricultura Sinica    2025, 58 (9): 1663-1683.   DOI: 10.3864/j.issn.0578-1752.2025.09.001
    Abstract319)   HTML56)    PDF (9359KB)(971)       Save

    【Objective】 Wheat is a cornerstone of global food security, with its production being pivotal in both China and the international community. With global climate change, the threat of high temperature has become increasingly prominent, posing a significant challenge to wheat cultivation. The strategic identification and selection of heat-tolerant germplasm, coupled with the exploration of genes associated with heat resistance, are crucial steps. These efforts are essential for broadening the genetic diversity of heat tolerance in wheat within China, providing prerequisites for breeding heat-tolerant wheat varieties and ultimately contributing to the safeguarding of our nation’s food security in the face of a warming climate. 【Method】 In this study, a natural population of 331 wheat accessions was utilized, and artificial climate chambers were employed to simulate high temperatures conditions. The heat tolerance of wheat seedlings was assessed by monitoring their survival rate under various durations of treatment, using heat resistance grade as the evaluative metric. Meanwhile, a genome-wide association study (GWAS) was conducted using the 55K SNP chip to identify genetic loci associated with heat tolerance. Expression data from multiple tissues, including roots, leaves under heat stress were analyzed, leading to the selection of genes related to heat tolerance. Subsequently, qPCR validation of candidate genes was performed using the extremely heat-tolerant accession Xinong 889 and the heat-sensitive accession Chinese Spring (CS) as materials. 【Result】 Under high-temperature stress, significant variations in survival rates were observed among different wheat accessions. The extremely heat-tolerant, moderately heat-tolerant, moderately heat-sensitive, and extremely heat-sensitive germplasm accounted for 110, 104, 110, and 7, respectively, representing 33.23%, 31.42%, 33.23%, and 2.12% of the total. Heat-tolerant germplasms, including Xinong 889, Zhengmai 7698, Zhongmai 895, Zhoumai 18, and Fengchan 3, were identified. Through GWAS, a total of 293 SNP loci significantly associated with the 12-hour survival rates (SR) and heat resistance grades (HRG) were detected, with the phenotypic variation explained ranging from 4.40% to 12.46%. Among these, 200 loci were related to the 12-hour survival rates, and 257 were related to the heat resistance grades, with 164 loci identified as the same heat-related loci. Based on significantly associated SNP markers, 313 heat-related genes were predicted. According to gene annotation information and expression data under heat stress, 23 heat tolerance candidates were selected, and after qPCR validation of differentially expressed candidate’s genes, 20 key heat tolerance candidate genes were identified. 【Conclusion】 At the seedling stage, 331 wheat germplasms were identified for heat tolerance. A rapid method was developed for determining the survival rate of wheat seedlings subjected to treatments of varying durations at 45 ℃ to assess their heat tolerance In total, 38 heat-tolerant germplasms and 293 loci significantly associated with seedling heat tolerance were screened. Also, TraesCS1A02G355900, TraesCS1A02G389500, TraesCS5A02G550700, TraesCS5D02G557100, TraesCS6D02G402500 and TraesCS7A02G232500 represented as candidate genes were filtered out.

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    Characteristics and Technical Approaches of Integrated Unmanned High-Yield Cultivation of Wheat
    ZHANG HongCheng, XING ZhiPeng, ZHANG RuiHong, SHAN Xiang, XI XiaoBo, CHENG Shuang, WENG WenAn, HU Qun, CUI PeiYuan, WEI HaiYan
    Scientia Agricultura Sinica    2025, 58 (5): 864-876.   DOI: 10.3864/j.issn.0578-1752.2025.05.004
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    【Objective】The aim of this study was to provide the theoretical and technical support for the innovation of green, high-yield, high-quality and high-efficient unmanned cultivation technology system of wheat. 【Method】 According to the situation of accelerating land transfer and large-scale operation, decreasing labor force engaged in agricultural production, and more efficient and comfortable farming methods, the integrated unmanned cultivation technology of wheat was put forward through the integration study of “agronomy-machinery-intelligence”, that is, using new technology, new product and new equipment to simplify and integrate the whole process of wheat production, and complete wheat production with the least number of operations, the least number of machines and unmanned operations. On the basis of exploratory experimental research, the integrated unmanned cultivation technology of wheat (IU) and conventional mechanized high-yield cultivation techniques of wheat in experimental area (CK) were set up as treatments in Dazhong Farm of Yancheng, Jiangsu Province in 2019-2020, 2020-2021 and 2021-2022, to study the traits and differences of wheat yield formation among different technology treatments, analyze the high-yield traits of IU, and put forward the technical approaches of IU. 【Result】 The IU increased wheat yield by 3.0%-5.9% compared with CK, and significant differences were observed between treatments of some varieties or some growing seasons. In terms of yield components, the spike number was IU>CK (significant differences were observed between treatments of some varieties or some growing seasons), the grains per spike were IU>CK (P>0.05), the total grains were IU>CK (P<0.05), and the 1000-kernels weight was IU<CK (P>0.05), indicating that the IU increased wheat yield by stabilizing the grains per spike and 1000-kernels weight, and increasing the spike number. In the production of photosynthetic matter, the culm number, leaf area index, dry matter accumulation at the main growth stages, the leaf area duration and crop growth rate in the main growth periods, and the culm fertility and grain leaf ratio were all expressed as IU>CK (significant differences were observed between treatments of some varieties or some growing seasons), which laid a material foundation for the yield increase of the IU. This paper not only summarized the technical approaches and basic technologies of IU but also discussed the development of IU from the aspects of integrated cultivation, unmanned cultivation, “agronomy-machinery-intelligence” fusion degree, key agronomy technology and comprehensive evaluation. 【Conclusion】 The yield under IU was equivalent or significantly increased to that under CK. And the high-yield cultivation of wheat was realized with less agricultural machinery and labor and unmanned operation, which was an effective way for the development of agricultural modernization production. In the future, multi-faceted collaborative innovation and investment should be strengthened to accelerate the application and large-scale promotion of this technology.

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    Integrated Nutrient Management Technology and Its Effects for Annual Rice-Rapeseed Rotation
    REN Tao, FANG YaTing, LU JianWei
    Scientia Agricultura Sinica    2025, 58 (16): 3159-3163.   DOI: 10.3864/j.issn.0578-1752.2025.16.001
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    Evaluation of High Temperature Tolerance and Selection of Sesame (Sesamum indicum L.) Cultivars at Full Flowering Stage Based on Principal Components-Cluster Analysis
    RONG YaSi, LI Feng, ZHANG PengYu, WANG DongYong, SU XiaoYu, TIAN Yuan, GAO TongMei
    Scientia Agricultura Sinica    2024, 57 (20): 3957-3973.   DOI: 10.3864/j.issn.0578-1752.2024.20.002
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    【Objective】To understand the effects of high temperature stress on the agronomic traits, yield, its components and grain quality of sesame, to accurately evaluate the high temperature tolerance level of different sesame varieties at full flowering stage, and to screen the high temperature tolerant sesame varieties.【Method】24 sesame varieties were treated with normal temperature (CK) and high temperature stress(H) at full flowering stage for 7 days. The agronomic traits such as plant height, initial capsule site, number of capsules per plant, number of capsules per capsule, 1000-grain weight, yield per plant and quality indexes such as water content, oil content and protein content were measured at maturity stage. The heat tolerance of different sesame varieties at full flowering stage was comprehensively analyzed and evaluated by means of principal component and cluster analysis.【Result】Different varieties, temperature treatments and interactions had significant effects on the indexes of sesame. After high temperature stress, compared with CK, the yield per plant, 1000-grain weight, number of capsules per plant, number of capsules per capsule, initial capsule site, capsule length and plant height of sesame decreased by 33.47%, 13.62%, 7.76%, 5.75%, 2.61%, 1.64% and 1.40%, respectively. Among which 1000-grain weight and yield per plant decreased the most, and the differences reached significant level. The difference of end length and protein content was no significant, which was 13.43% and 1.55% lower than that of the CK. The water content and oil content increased by 0.90% and 0.17% respectively compared with CK, and the difference was not significant. There is a certain correlation between the high temperature tolerance coefficients of different varieties and various indexes. The high temperature resistance coefficient was analyzed by principal component analysis, and five independent comprehensive indexes were established, and the contribution rates were 30.74%, 25.82%, 13.18%, 10.12% and 8.33%, respectively, reflecting 88.19% of the original information. The weight of each index was calculated by component matrix and eigenvalue, and the physiological comprehensive index model of high temperature stress (HTSPCI) was constructed. According to the cluster heat map analysis of high temperature tolerance coefficient of each individual index, 24 sesame germplasm resources were divided into 4 grades: high temperature resistant type, medium high temperature tolerance type, medium heat sensitive type and heat sensitive type, respectively. And the yield loss rate per plant (X6) of 6 high temperature resistant varieties was ≤9.50%, 6 medium high temperature resistant varieties was 9.50%<X6≤30%, 10 medium heat sensitive varieties was 30%<X6≤70% and 2 heat sensitive varieties was X6>70%.【Conclusion】High temperature treatment significantly affected the growth and development of sesame at full flowering stage. A comprehensive index model of high temperature stress was established by combining agronomic traits and quality indexes with principal components-cluster analysis, and 6 varieties with high temperature tolerance were selected, which could be used to evaluate the high temperature tolerance of different sesame varieties.

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    Effects of Long-Term Mulching Practices on Maize Yield, Soil Organic Carbon and Nitrogen Fractions and Indexes Related to Carbon and Nitrogen Pool on the Loess Plateau
    ZHANG FangFang, SONG QiLong, GAO Na, BAI Ju, LI Yang, YUE ShanChao, LI ShiQing
    Scientia Agricultura Sinica    2025, 58 (3): 507-519.   DOI: 10.3864/j.issn.0578-1752.2025.03.008
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    【Objective】The aim of this study was to clarify the effects of long-term mulching practices on crop yield, soil carbon and nitrogen physical fractions and carbon and nitrogen pool-related indexes, with a view to providing a scientific basis for long-term maintenance of high crop yields and soil fertility in dry-crop farmlands on the Loess Plateau. 【Method】 Based on a field experiment of more than 10 years in Changwu County, Shaanxi Province, three treatments of no mulching (CK), gravel mulching (GM) and film mulching (FM) were set up. From 2018 to 2020, in situ soil samples in the 0-20, 20-40 and 40-60 cm soil layers were collected, and soil samples from each soil layer were grouped by using the physical method. The variation characteristics were investigated, including spring maize yield, and soil organic carbon (SOC), total nitrogen (TN), particulate organic carbon (POC), particulate organic nitrogen (PON), mineral-bound organic carbon (MAOC) and mineral-associated organic nitrogen (MAON) in each soil layer. Based on carbon pool management index (CPMI), nitrogen pool management index (NPMI), and carbon stability index (CSI), the nitrogen stability index (NSI). Combined with correlation analysis, the relationships between the yields and the soil carbon and nitrogen fractions and the related indexes were clarified. 【Result】Compared with CK treatment, the GM treatment showed a decrease in yield, with a 5.8% decrease in mean yield, while the FM treatment continued to increase yield, with a significant 13.6% increase in mean yield. The mean SOC and TN content of the topsoil (0-20 cm) were decreased under both the GM and FM treatments compared with CK treatment, with a significant reduction of 7.3% in the mean SOC content of the topsoil under FM treatment, while it was not significant under GM treatment. Both GM and FM treatments significantly decreased mean POC and PON content in the topsoil and significantly increased MAOC and MAON content in the topsoil compared to CK treatment, i.e. long-term mulching practices significantly decreased labile carbon and nitrogen contents in the topsoil and significantly increased recalcitrant carbon and nitrogen content in the topsoil. Compared with CK treatment, the GM treatment significantly decreased CPMI in the topsoil and significantly increased CSI in the topsoil, whereas the FM treatment significantly decreased CPMI and NPMI and significantly increased CSI and NSI in the topsoil, indicating that the GM treatment significantly increased carbon pool stability of the topsoil, whereas the FM treatment significantly increased both carbon pool stability and nitrogen pool stability of the topsoil. Correlation analyses showed that in addition to POC and MAOC being related to carbon pool indexes, POC was also closely related to NPMI and NSI, while MAOC was also closely related to yield and NPMI. 【Conclusion】Long-term film mulching maintained high crop yields and improved the stability of soil carbon and nitrogen pools, but it reduced topsoil fertility and could be made more sustainable by combining it with other measures to replenish soil organic matter.

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    Effects of Long-Term Fertilization on Nitrogen Surplus and Deficit and Soil Alkali-Hydrolyzed Nitrogen in Red Soil Double-Cropping Rice System
    HU DanDan, SONG HuiJie, DUAN YingHua, WU Yan, HU ZhiHhua, XU XiaoLin, ZHANG WenJu, HE XiaoLin, LIU KaiLou, SU Peng, HUANG QunZhao
    Scientia Agricultura Sinica    2024, 57 (24): 4907-4918.   DOI: 10.3864/j.issn.0578-1752.2024.24.007
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    【Objective】The analysis of rice yield, nitrogen use efficiency and the quantitative relationship between apparent nitrogen balance and soil alkali-hydrolyzed nitrogen under different nitrogen fertilizer management conditions could provide a more comprehensive understanding of the effects of long-term fertilization on soil fertility, so as to provide the theoretical guidance for efficient production and scientific nitrogen management of red soil paddy fields. 【Method】Based on the red soil double cropping rice long-term fertilization positioning experiment (started in 1981, located in Jinxian County, Jiangxi Province), five treatments were selected: no fertilizer (CK), nitrogen and phosphorus fertilizer (NP), nitrogen and potassium fertilizer (NK), nitrogen, phosphorus and potassium fertilizer (NPK), nitrogen, phosphorus and organic fertilizer (NPKM), and then the grain and straw yield and nitrogen uptake of rice in each season were investigated and analyzed, and the soil alkali-hydrolyzed nitrogen content was analyzed after late rice. The nitrogen uptake, nitrogen utilization rate, nitrogen apparent balance and the changes of soil alkali-hydrolyzed nitrogen were calculated and analyzed on a 10-year basis. 【Result】During the 40 years of experiment (1981-2020), the rice yield and nitrogen uptake under NPKM treatment were the highest, increased by 65.9%-108.4% and 85.1%-132.5% compared with CK, respectively, and increased by 19.3%-92.1% and 19.4%-99.8% compared with fertilizer treatments (NPK, NK and NP), respectively, showing significant differences. With the increase of the experimental period, the nitrogen use efficiency of fertilizer treatment gradually decreased, and the NPKM treatment also showed a decreasing trend in the first 30 years (1981-2010), but the rate was slower than that of fertilizer treatment, and increased in the recent 10 years (2011-2020), and from the lowest in the first 10 years (1981-1990) to the highest in the recent 10 years, increased by 25.3%-271.2% compared with fertilizer treatment. The nitrogen surplus was the highest under NPKM treatment during the 40 years of experiment, with an increase of 137.1%-577.2% compared with fertilizer treatment, but in the last 30 years (1991-2020), the nitrogen surplus gradually decreased with the increase of the experimental period. The soil alkaline hydrolyzed nitrogen content was the highest under NPKM treatment during the 40 years of experiment, increased by 7.1%-24.4% compared with CK, but the difference was not significant in the first 10 years, and increased by 11.0%-35.2% compared with fertilizer treatment, while there was no significant difference between fertilizer treatment and CK. Correlation analysis showed that the nitrogen surplus was significantly positively correlated with the soil alkaline hydrolyzed nitrogen content in the last 20 years (2001-2020). 【Conclusion】In the red soil double-cropping rice system, with the increase of fertilization years, the combined application of organic and inorganic fertilizers had better effects on rice yield, nitrogen uptake, nitrogen uptake and utilization, and soil alkali-hydrolyzed nitrogen content. Meanwhile, the increase of nitrogen surplus caused by long-term fertilization also further increased the soil alkali-hydrolyzed nitrogen content in the topsoil. The contribution capacity of nitrogen surplus to soil alkali-hydrolyzed nitrogen increased gradually.

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    Effects of miRNA on Gene Expression of Sphingolipids Metabolism and Small RNA Analysis of Silencing NlSPT1 and NlSMase4 in Nilaparvata lugens
    WANG Ni, SHI ZheYi, YOU YuanZheng, ZHANG Chao, ZHOU WenWu, ZHOU Ying, ZHU ZengRong
    Scientia Agricultura Sinica    2024, 57 (20): 4022-4034.   DOI: 10.3864/j.issn.0578-1752.2024.20.008
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    【BackgroundSphingolipids are the second major type of membrane lipids and mediate various biological processes as signal transducers, including cell growth, development, reproduction and apoptosis. Sphingolipid metabolism is tightly regulated by sphingolipid metabolizing enzymes to maintain the homeostasis of metabolism in vivo.aaaaa【Objective】The objectives of this study are to investigate the relative transcript levels of genes related to the sphingolipid metabolism pathway after silencing of the core components of microRNA (miRNA) biosynthesis pathway, NlAgo1, NlDicer1 and NlDrosha by RNA interference (RNAi), and analyze the differentially expressed miRNAs after silencing of serine palmitoyltransferase 1 (SPT1) and sphingomyelinase 4 (SMase4) gene combined with small RNA sequencing of Nilaparvata lugens, explore the role of miRNAs in the sphingolipid metabolism of N. lugens, and to provide a new molecular target for pest control.【Method】RNAi was performed with double stranded RNAs (dsRNAs) targeting NlAgo1, NlDicer1 and NlDrosha at 1 day post adult eclosion (1 PAE), respectively, and dsGFP was used as control. The ovaries at 5 PAE were dissected and β-actin was used as internal reference gene, the transcript levels of genes related to the sphingolipid metabolism pathway were detected by reverse-transcription quantitative PCR (qRT-PCR). miRNAs that may regulate the expression of NlSPT1 and NlSMase4 were predicated based on small RNA libraries combined with miRNA-target prediction software. Differentially expressed miRNAs after the silencing of NlSPT1 and NlSMase4 were identified and the target gene enrichment was analyzed by small RNA sequencing.【Result】RNAi-mediated silencing of NlAgo1, NlDicer1 or NlDrosha significantly up-regulated the expression of genes related to the sphingolipid metabolism pathway, including NlSPT1 and NlSMase4 in ovaries. Target gene prediction revealed 6 miRNAs and 13 miRNAs that could bind NlSPT1 and NlSMase4. The target genes of differentially expressed miRNAs that silencing NlSPT1 and NlSMase4 were significantly enriched in biological processes, including nuclear and protein binding, as well as metabolic pathways such as endocytosis, endoplasmic reticulum processing, MAPK signaling pathway, TOR signaling pathway, apoptosis, and lipid metabolism.【Conclusion】NlAgo1, NlDicer1, and NlDrosha-dependent miRNAs affect sphingolipid metabolism by influencing the expression of genes encoding sphingolipid metabolizing enzymes. The silencing of NlSPT1 and NlSMase4 induced changes in miRNA expression levels in N. lugens ovaries. These research results can provide a theoretical basis for pest control based on sphingolipid metabolism genes.

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    Mining and Utilization of Crop Disease Resistance and Insect-Related Genes
    HE NiQing, LIN ShaoJun, CHENG ZhaoPing, YANG DeWei
    Scientia Agricultura Sinica    2024, 57 (23): 4589-4592.   DOI: 10.3864/j.issn.0578-1752.2024.23.001
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    Establishment of Rapid Field Co-Detection Method of ASFV Antibody and Nucleic Acid Based on Quantum Dot Microspheres and RPA Technology
    ZHAO YiRan, SHAN YanKe, LI JiaHao, HE ZhaoQun, WANG XinYi, WEN Dun, WANG MiLa, CHU Rui, ZHAO DongMing, LIU Fei
    Scientia Agricultura Sinica    2024, 57 (24): 4990-5002.   DOI: 10.3864/j.issn.0578-1752.2024.24.013
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    【Background】African swine fever (ASF), recognized as a notifiable disease by the World Organization for Animal Health (WOAH) and categorized as a Class I animal disease in China, presents significant challenges due to the absence of effective vaccines and treatment options. Therefore, early, effective, rapid, and sensitive detection on ASF is crucial for controlling ASF. Current clinical diagnostic methods for African swine fever virus (ASFV) primarily include quantitative real-time PCR (qPCR) and enzyme-linked immunosorbent assay (ELISA). However, these techniques require complex procedures, expensive equipment, and extended detection times, rendering them unsuitable for rapid field testing. Moreover, the levels of antibodies and nucleic acids in the host exhibit significant variations over time following ASFV infection, which can result in false negatives if only ASFV nucleic acids or antibodies are tested in isolation. 【Objective】This study aimed to develop a test strip that allowed for the simultaneous detection of ASFV antibodies and nucleic acids based on quantum dot microspheres and recombinase polymerase amplification (RPA) technology. The objective was to enable on-site detection of both ASFV antibodies and nucleic acids, thereby enhancing the sensitivity and specificity of the tests, reducing detection costs, and saving time. 【Method】In this study, a lateral flow immunoassay strip for the simultaneous detection of ASFV antibodies and nucleic acids was developed by combining quantum dot fluorescence immunochromatography technology with recombinase polymerase amplification (RPA). The corresponding portable fluorescence analyzer enables rapid on-site dual detection of ASFV antibodies and nucleic acids. The study optimized key parameters, such as the quantum dot-protein conjugation amount and the coating concentration of the test line. Based on these optimizations, the cutoff value, sensitivity, specificity, and reproducibility of the detection method were determined. Furthermore, the developed ASFV dual detection strip was compared with commercially available ELISA and qPCR kits by testing clinical samples to assess detection consistency, validating the method's concordance, and evaluating the practical applicability of the test strip. 【Result】The dual detection strip was user-friendly and rapid, with the entire testing process completed within 30 minutes. It demonstrated no cross-reactivity with six other common porcine infectious viruses, confirming excellent specificity. The sensitivity for nucleic acid detection reached 10 copies/µL, while the sensitivity for antibody detection was up to 1﹕3 200. The intra-assay variability coefficients for both antibody and nucleic acid tests were less than 10%, and the inter-assay variability coefficients were less than 15%, indicating excellent reproducibility. Validation against commercial ELISA and qPCR kits yielded a conformity rate of 100%. Furthermore, in clinical sample testing, two samples tested positive for antibodies but negative for nucleic acids in the corresponding pigs, which exhibited no significant clinical symptoms. These findings further confirmed that the combined detection of antibodies and nucleic acids could enhance the accuracy of testing and reduce the incidence of false negatives. 【Conclusion】The cost per experiment for this nucleic acid-antibody dual detection method was lower compared with the total cost of separate nucleic acid and antibody testing, thus reducing both detection costs and time. It was sensitive to both acute outbreaks and chronic infections of ASF, addressing the limitations of detecting only nucleic acids or antibodies individually. The method had minimal requirements for operating conditions, and the portable fluorescence analyzer was easy to use, requiring neither expensive laboratory equipment nor highly specialized personnel. This reduced the precision demands on detection devices, making it suitable for ASFV testing in remote areas and at the grassroots level. Therefore, this method held great potential as a reliable tool for point-of-care ASFV detection in pig farms in the future.

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    Analysis of Problems and Pathways for Increasing Cotton Yield per Unit Area in Xinjiang Under Green and Efficient Production Mode
    TIAN LiWen, LOU ShanWei, ZHANG PengZhong, DU MingWei, LUO HongHai, LI Jie, PAHATI MaiMaiTi, MA TengFei, ZHANG LiZhen
    Scientia Agricultura Sinica    2025, 58 (6): 1102-1115.   DOI: 10.3864/j.issn.0578-1752.2025.06.005
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    Cotton production in Xinjiang is transforming from high input of production materials to green, high efficiency and light simplification. This paper analyzed the existing issues as well as advantages and disadvantages of cotton production in Xinjiang under the green and high efficiency mode of production, so as to understand the current situation of cotton production in Xinjiang, explore ways and means to improve the yield per unit area, and put forward suggestions and countermeasures for improving the yield per unit area. This paper provided a detailed analysis of the current status of green and efficient cotton production in Xinjiang in recent years, focusing on resource conditions, mechanization levels, and management practices. Specifically, the utilization rate of chemical fertilizers was close to 40%, the area under drip irrigation exceeded 1.86 million hm2, the recovery rate of plastic films from farmland in the current season reaches 80%, the comprehensive mechanization rate for tillage, sowing, and harvesting attained 94.5%, locally developed cotton varieties accounted more than 90% of the market share across Xinjiang, and the average managed area per cotton farmer reached 20 hm2. These achievements demonstrated efficient resource utilization, enhanced production efficiency, and standardized and normalized management. However, these challenges remained, which included drip irrigation systems and technologies that lagging behind the demands of modern agriculture, excessive inputs of fertilizers, pesticides, and plastic films, inadequate implementation of technologies with poor alignment with regional needs, and a lack of environmental-friendly concepts among cotton farmers. Therefore, it was quite challenging to maintain green and efficient production while also increasing cotton yield per unit area. It was recommended to improve the efficiency of water utilization by modifying drip irrigation systems and refining technological models, fostering superior crop populations to boost yield. We should optimize the input of fertilizers, pesticides, and plastic films, apply fertilizers rationally, prevent the abuse of pesticides, and intensify the management of residual plastic films, supporting yield enhancement through the improvement of resources and the environment. Efforts should be made to ensure the implementation of technologies aligned with regional needs, advocating for green development concepts and guaranteeing sustainable yield enhancement through changes in management and mindset. To achieve high-quality development of cotton in Xinjiang, it was essential to leverage regional characteristics and scale advantages, making production more specialized. We should refine, improve, integrate, and innovate technologies in production details, promoting sustained and healthy economic and ecological development in Xinjiang's cotton regions through green and efficient cotton planting.

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    Effects of LED Supplementary Lighting on Production and Leaf Physiological Properties of Substrate-Cultivated Strawberry in Chinese Solar Greenhouse
    ZHANG Han, ZHANG YuQi, LI JingLai, XU Hong, LI WeiHuan, LI Tao
    Scientia Agricultura Sinica    2025, 58 (5): 975-990.   DOI: 10.3864/j.issn.0578-1752.2025.05.012
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    【Objective】The objective of this research is to clarify the effects of LED supplementary lighting on production and leaf physiological characteristics of substrate-cultivated strawberry, and develop a light control strategy for strawberry cultivation in Chinese solar greenhouses, which will provide theoretical basis and technical support for improving the quality and efficiency of strawberry cultivation in winter and spring seasons in China when solar radiation is low.【Method】Strawberry cultivar ‘HongYan’ was grown in a Chinese solar greenhouse with substrate cultivation, and LED supplementary lighting was provided during the early stage of flower bud differentiation (lamps were installed approximately 15 cm above the canopy). The experiments were set up with different light intensity experiments (photosynthetic photon flux density (PPFD) of 254, 367, and 492 μmol·m-2·s-1, corresponding to the power of 80, 120 and 160 W, respectively), the different light quality experiments (red/blue 9/1, red/blue 1/1, and white light, PPFD of 360-390 μmol·m-2·s-1, with the same power of 120 W), and the different supplementary lighting duration and control strategy experiments (i.e. dynamic supplementary lighting for 10 h and continuous supplementary lighting for 5 h, referred to as DL10 and CL5 hereafter, respectively, both using 120 W white LED, PPFD of 367 μmol·m-2·s-1, lamp on/off strategy of DL10 treatment was the same as the light intensity and quality experiments, lamp of CL5 treatment was continuously turned on during the time period of 8: 00-13: 00), and the control was no supplementary lighting treatment. During the experiment, strawberry production, physiological and biochemical index of leaves and fruits, as well as the leaf photosynthetic parameters were measured, and the power usage efficiency was also analyzed.【Result】Compared with the control, all supplementary lighting treatments increased strawberry yield and accelerated harvest time by ~10 d. In the light intensity experiment, the yield of 160 W treatment increased by 41.9%, which was slightly but not significantly higher than that of 80 W and 120 W treatments. In the light quality experiment, the yield of red/blue 9/1, red/blue 1/1 and white light treatments increased by 55.9%, 44.1%, and 33.1%, respectively, compared to the control. In addition, the yield of DL10 treatment increased by 16% compared to CL5 treatment. Supplementary lighting increased yield due to the higher number of fruits per plant. Supplementary lighting reduced fruit water content and increased leaf thickness, but had no significant effect on leaf physiological and biochemical parameters. Supplementary lighting in the morning and afternoon significantly improved stomatal conductance, which was beneficial for photosynthesis. However, in the light intensity experiment, the maximum photosynthetic capacity of the leaves treated with 160 W was significantly lower than that of 120 W treatment, and the stomatal conductance was also lower than that of the control. Regarding the power usage efficiency, red/blue 9/1 (120 W) treatment was the highest, while the 160 W white light was the lowest among all treatments. The power usage efficiency of DL10 treatment was 2.6 times that of CL5 treatment.【Conclusion】Supplementary lighting can significantly improve strawberry production and accelerate harvest time in winter and spring seasons when solar light is limited, appropriate supplementary light intensity is crucial for yield formation, and supplementing with a high fraction of red light has the best effect on strawberry production, dynamic supplementary light control strategy can significantly improve the power usage efficiency.

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    Effects of Different Nitrogen Forms on Yield and Quality of Summer Maize
    XUE YuQi, ZHAO JiYu, SUN WangSheng, REN BaiZhao, ZHAO Bin, LIU Peng, ZHANG JiWang
    Scientia Agricultura Sinica    2025, 58 (8): 1535-1549.   DOI: 10.3864/j.issn.0578-1752.2025.08.006
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    【Objective】 The effects of different nitrogen forms on filling characteristics, grain quality and yield of summer maize were studied, so as to provide the scientific basis for selecting suitable nitrogen fertilizer types and improving the yield and grain quality of summer maize. 【Method】 The experiment was conducted in Taian, Shandong Province from 2022 to 2023. Denghai 605 (DH605) was selected as the experimental material, with a nitrogen application rate of 210 kg N·hm-2. The experiment included five treatments: amide nitrogen (Urea, UREA), nitrate nitrogen (Calcium nitrate, NN), ammonium nitrogen (Ammonium chloride, AN), co-application of nitrate and ammonium nitrogen (1:1, HH), and urea ammonium nitrate solution with a blend of amide nitrogen, nitrate nitrogen, and ammonium nitrogen (2:1:1, UAN). The effects of different nitrogen forms on the yield and quality of summer maize were investigated by determining the grain filling characteristics, grain quality characteristics and grain capacity of summer maize. 【Result】Compared with the conventional application of amide nitrogen in UREA, both the maize yield and grain quality under NN decreased. The maize yield under AN increased, but the grain quality decreased. HH significantly increased maize yield without affecting grain quality. UAN significantly increased maize yield and improved grain quality. Over the two years, the highest maize yield achieved with the co-application of the three nitrogen forms, significantly increasing by 13.7% to 16.3% compared with UREA. The Next the highest maize yield were from AN and HH, which significantly increased maize yield by 5.2% to 6.8% and 7.3% to 10.6%, respectively, compared with UREA. The maize yield under NN decreased by 5.4% to 5.8% compared with UREA. Compared with UREA, the growth amount at the maximum filling rate (Wmax) under UAN was enhanced by 6.3% to 9.7%, and the active filling period (D) was extended by 7.7% to 10.9%. Both AN and HH increased Wmax and prolonged D, thereby promoting the accumulation of grain weight and increasing yield. The Wmax, D, grain filling rate, and dehydration rate of NN were significantly lower than those in the other treatments. The crude protein content was lower with NN and AN, decreasing by 20.6% to 22.0% and 15.2% to 17.4% than that under UREA, respectively. The rude fat content with NN was significantly higher than that of other treatments, increasing by 23.6% to 30.9% than that under UREA. Compared with UREA, UAN improved grain quality, with total starch and amylopectin content increasing by 4.9% to 5.2% and 11.7% to 14.4%, respectively, compared with UREA, and the ratio of amylopectin to amylose increased by 31.0% to 39.1%. The amylose content decreased by 14.1% to 16.8%. The crude protein content of UAN increased by 11.7% to 24.1%. The grain bulk weight under UAN was significantly higher than that under other treatments. 【Conclusion】Compared with the conventional application of amide nitrogen, the treatment with nitrate nitrogen inhibited grain filling, reduced grain weight, and decreased yield. In contrast, ammonium nitrogen or the co-application of multiple nitrogen forms enhanced the grain filling process, increased grain weight, and thereby improved yield. Furthermore, compared with the application of a single nitrogen form, the co-application of three nitrogen forms could achieve a synergistic improvement in both yield and grain quality.

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