Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (3): 415-425.doi: 10.3864/j.issn.0578-1752.2015.03.02

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Evolution of Genetic Distance Between Parental Lines of Chinese Sorghum Hybrids from1970s-2000s Based on SSR Analysis

WANG Rui1,2, WANG Jin-sheng1, ZHANG Fu-yao2, CHENG Qing-jun2, TIAN Cheng-hua2, LING Liang3   

  1. 1College of Life Sciences, Shanxi Agricultural University, Taigu 030801, Shanxi
    2Sorghum Institute, Shanxi Academy of Agricultural Sciences/ Key Laboratory of Genetic and Germplasm Innovation in sorghum for Shanxi Province, Yuci 030600, Shanxi
    3Institute of Edible Fungi, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, Shanxi
  • Received:2014-06-26 Online:2015-01-31 Published:2015-01-31

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Abstract: 【Objective】To explore the genetic differences of Chinese sorghum varieties and evolution of the superior groups since heterosis utilization, SSR markers were used to identify the genetic distance of sorghum parental lines and F1. Genetic background of Chinese sorghum main varieties in different periods were analyzed, and systematically understand of the genetic relationship, genetic diversity and genetic distance of sorghum hybrids and their parental lines can reduce the blindness of parental lines selection, effectively improve the predictability of cross breeding.【Method】SSR genetic distance of the representative hybrids and their parental lines grown in late-maturing area since 1970s were analyzed. Analysis of the superior groups of sorghum hybrids, research of the breeding progress and the evolution characteristics since heterosis utilization of sorghum were conducted at molecular level. 【Result】 A total 55 hybrids and their parental lines were evaluated by 109 pairs of SSR markers. The results revealed that 47 pairs of SSR markers were highly polymorphic. 373 varied alleles were amplified with an average of 7.5 alleles per marker, the ranges were 2 to 14. The ranges of PIC in 55 cultivars were 0.0351-0.8836, with an average of 0.6085. The genetic distance was averaged at 0.6011 with a range from 0.0889 to 0.9500. The sorghum materials were classified into 4 groups based on the SSR experiments. The clustering results were similar to those based on geographical origins and genetic backgrounds. The results showed that the average genetic distance between the two parental lines of hybrids from different eras showed a upward trend in 1970-1980, then a slight decline. According to the analysis of genetic distance between the two parental lines of hybrids from different heterosis models, Durra sterile lines×Kaoliang or trend Kaoliang restorer lines and Kafir-caudatum sterile lines×Kaoliang or trend kaoliang restorer lines have a far genetic distance, high yield, therefore, the heterosis model of Chinese sorghum hybrids should be Durra×kaoliang or trend kaoliang, Kafir-caudatum×kaoliang or trend kaoliang. 【Conclusion】In this study, it was found that the genetic distance between parental lines has a close relationship with heterosis level in sorghum, therefore, genetic distance should be fully considered in the matching of sorghum parental lines.

Key words: sorghum, SSR, genetic distance, heterosis

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