不同环境下水稻株高和穗长的QTL分析

doi:10.3864/j.issn.0578-1752.2015.03.01

Abstract

Abstract: 【Objective】Panicle length and plant height are two important factors affecting rice production. Breeding varieties with large panicle and the ideal plant architecture traits are significant for increasing the grain yield of rice. To explore the genetic basis of plant height and panicle length in rice, quantitative genetic analysis was conducted in three different environments. In this study, stable expressed QTLs for plant height and panicle length were identified. The results of this study help us to understand the genetic basis of plant height and panicle length, and provide us with useful information for marker-assisted improvement of the plant type in rice.【Method】The recombinant inbred lines (RILs) derived from the cross between Shennong 265 (japonica) and Lijiangxintuanheigu (japonica) consisting of 126 lines were used to identify QTLs for plant height and panicle length in three different environments (Shenyang, 2011; Hainan, 2012; Shenyang, 2013). Nine QTLs were mapped by QTL IciMapping v3.0 software, using ICIM method for plant height and panicle length. In addition, based on the QTL results and the published literature and rice database data, the major QTLs in 3 environments were analyzed. These results were further confirmed the reliability of the QTL. Finally, in order to narrow the interval of the major QTLs, the QTL-BSA(Bulked Segregant Analysis of Major QTL) was used fine mapping.【Result】Analysis of variance test showed that a significant difference was detected between the parents under three different conditions. A normal distribution of phenotypic values for panicle length and plant height were observed, which indicated that panicle length and plant height were controlled by multiple genes. A total of nine QTLs for the plant height and panicle length were mapped. Five QTLs affecting plant height were detected on the chromosomes 6, 7, 9 and 12, with the LOD values ranged from 2.67 to 19.39, the additive effect from -17.68 to 2.90, and the range of individually explaining phenotypic variation was from 4.25% to 37.35%. A total of four QTLs for the panicle length were mapped on the chromosomes 6, 7 and 9, with the LOD values ranged from 3.57 to 23.18, the additive effect from -3.22 to 1.42, and the range of individually explaining phenotypic variation was from 11.30% to 61.62%. Of these, five were identified in more than one environments, four were significant in two or three environments, indicating that these QTLs are stable across years or environments. Among them, qPL9a and qPH9 were detected on the same region of chromosome 9 in three environments. qPH7 and qPL7b were mapped on the same interval of chromosome 7 in two or three environments, respectively. These QTLs’ alleles from LTH parent increased plant height and panicle length in this population. In addition, the analysis of major QTL-BSA mapping narrowed the qPHL9 (qPH9 and qPL9a)to a 522.46 kb region flanked by simple sequence repeat marker RM1189 and RM24457. qPHL7 (qPH7 and qPL7b) was mapped to a 856.49 kb interval flanked by simple sequence repeat marker RM478 and RM429 on chromosome 7. 【Conclusion】In this study, the recombinant inbred lines (RILs) derived from the cross between Shennong 265 (japonica) and Lijiangxintuanheigu (japonica) were used for mapping QTLs of plant height and panicle length. Five QTLs affecting plant height and four QTLs affecting panicle length were identified. Pleiotropic QTL region on the chromosome 9 could be detected in three environments. A novel plant height and panicle length pleiotropic QTL-qPHL7 was located on chromosome 7 in two environments.

Key words: rice, plant height, panicle length, QTL

YAO Xiao-yun, LI Qing, LIU Jin, JIANG Shu-kun, YANG Sheng-long, WANG Jia-yu, XU Zheng-jin. Dissection of QTLs for Plant Height and Panicle Length Traits in Rice Under Different Environment[J].Scientia Agricultura Sinica, 2015, 48(3): 407-414.

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