高粱遗传转化研究进展

doi:10.3864/j.issn.0578-1752.2024.03.003

Abstract

Abstract:

Sorghum is the fifth largest grain crop in the world and can be used for food, feed, brewing and bioenergy. Sorghum genetic transformation technology is an essential and important tool in the research of sorghum functional genomics and can also serve as an important complement to traditional breeding methods. In this review, we summarize the research progress of sorghum transformation in recent years, analyze the problems in sorghum genetic transformation and propose strategic solutions to them in order to provide a reference for further improvement of sorghum genetic transformation technology. By summarizing more than 50 literatures on sorghum tissue culture and genetic transformation in recent years, we introduced the current research status of sorghum genotypes, explant sources, and regeneration system construction for genetic transformation, and compared the advantages and disadvantages of four commonly used methods for sorghum genetic transformation: electroporation, pollen-mediated transformation, particle bombardment and Agrobacterium-mediated transformation, summarized the effects of the main components of genetic transformation vectors, including promoters, target genes, selective marker genes and reporter genes, on transformation efficiency, explained the current application status of sorghum genetic transformation, analyzed the main bottleneck problemns in sorghum genetic transformation technology, and studied countermeasures. Sorghum genotypes have a significant influence on tissue culture and P898012 and Tx430 are the most widely used. Gene bombardment and Agrobacterium-mediated transformation are the most commonly used methods for sorghum genetic transformation, and the advantages of Agrobacterium-mediated transformation are gradually emerging. In vector construction, CaMV35S and ubi1 are the most commonly used promoters, and antibiotic resistance genes (nptII, hpt), herbicide resistance genes (bar), and nutrient assimilation genes are the three commonly used selection markers. With the development of sorghum genetic transformation technology and CRISPR/Cas9-mediated gene editing technology, some genes with important agronomic traits have been successfully transferred into sorghum. However, strong genotype dependence, long tissue culture cycle, and poor genetic transformation stability are the main bottlenecks that limit the genetic transformation of sorghum. By introducing morphogenesis regulatory factors, somatic cell generation can be directly performed, which shortens the tissue culture cycle, improves the transformation efficiency, and expands the source of explants. This has become a major breakthrough in sorghum genetic transformation technology. The use of morphogenesis regulatory factors and adoption of cut-dip-budding (CDB) delivery system can further improve the sorghum genetic transformation technology. Combined with the application of CRISPR/Cas9 gene editing technology, they will surely provide an important technical basis for the sorghum molecular breeding and gene function identification.

Key words: sorghum, transformation technology, regenerative system, transformation method, vector

HAN LiJie, CAI HongWei. Progress on Genetic Transformation of Sorghum[J].Scientia Agricultura Sinica, 2024, 57(3): 454-468.

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Figures/Tables 1

Table 1

The research progress of sorghum genetic transformation"

转化方法 Transformation method	基因型 Genotype	外植体 Explant	载体 Vector	选择标记 Selectable marker	启动子 Promoter	筛选剂及筛选压 Screening agent and screening pressure	报告基因 Report gene	转基因检测 Transgenic detection	转化效率 Transformation efficiency (%)	参考文献 References
电穿孔法 Electroporation	-	原生质体 Protoplast	p35SCAT/ pCopa-CAT	-	CaMV35S copia promoter of Drosophila	氯霉素 Chloramphenicol	-	Scintillation counting	-	[30]
电穿孔法 Electroporation	NK300	原生质体 Protoplast	pBI221.1	nptⅡ	CaMV35S	100 mg·L^-1卡那霉素 Kanamycin	GUS	Gel blot hybridization	-	[31]
电穿孔法 Electroporation	Sorghum vulgare (cv Grazer)	细胞悬浮液 Cell suspension	pBC1/pNGI	nptⅡ/hpt	adh1/CaMV35S	500 mg·L^-1卡那霉素Kanamycin/50 mg·L^-1 潮霉素Hygromycin	GUS	GUS assay/ Gel blot hybridization	-	[32]
花粉管通道法 Pollen-mediated transformation	TX622B	花柱 Style	-	nptⅡ	-	100 mg·L^-1卡那霉素 Kanamycin	-	丝黑穗病鉴定/聚丙烯酰氨凝胶电泳 Head smut disease detection/ polyacrylamide gel electrophoresis	6.80	[33]
花粉管通道法 Pollen-mediated transformation	A（2）V4A/A（2）V4B	花粉 Pollen	pBI121	nptⅡ	CaMV35S	卡那霉素 Kanamycin	GUS	PCR/Southern blot	-	[34]
基因枪法 Particle bombardment	P898012	幼胚 Immature embryo	pPHP620/ pPHP687	bar	CaMV35S	3 mg·L^-1 bialophos	GUS	Southern blot/GUS assay	0.29	[7]
基因枪法 Particle bombardment	SRN39	幼穗 Immature infloresences	pPHP620	bar	CaMV35S	3 mg·L^-1 bialophos	GUS	Southern blot/GUS assay	2.61	[35]
基因枪法 Particle bombardment	Tx430	幼胚 Immature embryo	-	bar	CaMV35S	1-2 mg·L^-1 basta	-	Southern blot/Western blot/ PCR	0.09	[36]
基因枪法 Particle bombardment	M35-1/SA281/QL41/P898012	幼胚 Immature embryo	pAHC20	bar	ubi1/act1/ CaMV35S	2 mg·L^-1 bialophos	GFP	Southern blot/荧光显微镜 Southern blot/Fluorescence microscope	1.00	[9]
基因枪法 Particle bombardment	RTx430	幼胚 Immature embryo	pAHC20/ pAct1-D	bar	ubi1/act1	5 mg·L^-1 PPT	GUS	Southern blot/GUS assay	0.18	[37]
基因枪法 Particle bombardment	Tx430/C401/ CO25	幼胚 Immature embryo	pPZP200/ pUC18	hpt	ubi1/CaMV35S/HBT/act1/adh1	-	GUS/GFP	荧光显微镜/GUS assay Fluorescence microscope/ GUS assay	-	[5]
基因枪法 Particle bombardment	恢复系 Restorer line 5-27/115	幼穗 Immature infloresences	pKUB	hpt	-	20 mg·L^-1潮霉素 Hygromycin	GUS	PCR/Southern blot/ GUS assay	-	[38]
基因枪法 Particle bombardment	214856	幼胚/芽尖 Immature embryo/Shoot tip	pAct1-D/ pAHC25	nptⅡ	ubi1/adh1/act1D/ CaMV35S	25-100 mg·L^-1遗传霉素Geneticin	GUS	PCR/Southern blot/ GUS assay	1.30	[39]
基因枪法 Particle bombardment	BTx623	芽尖 Shoot tip	pJS108/ pmpiC1cry1Ac	bar	mpiC1	1-2.05 mg·L^-1 basta	GUS	PCR/Southern blot/ GUS assay	1.50	[28]
基因枪法 Particle bombardment	32个品种 Varieties	幼胚 Immature embryo	pPH1687	hpt	ubi1	40 mg·L^-1潮霉素 Hygromycin	LUC	Southern blot/荧光素酶测定 Southern blot/Luciferase camera assay	0.09	[40]
基因枪法 Particle bombardment	P898012	幼胚 Immature embryo	pAHC25/ pNOV3604	bar/pmi	ubi1	9 g·L^-1甘露糖 Mannose+12 g·L^-1 麦芽糖Maltose	GUS	PCR/Southern blot	0.77	[41]
基因枪法 Particle bombardment	Tx430	幼胚 Immature embryo	pUKN/pGEM-Ubi-gfp	nptⅡ	ubi1	30 mg·L^-1遗传霉素 Geneticin（G418）	GFP	PCR/Southern blot/荧光显微镜 PCR/Southern blot/ Fluorescence microscope	20.70	[6]
基因枪法 Particle bombardment	Hiro1	成熟胚 Mature embryo	pCAMBIA1301/p35S::SbGW2-GFP	hpt	ubi/CaMV35S	10 mg·L^-1 潮霉素 Hygromycin	GFP	PCR/荧光显微镜 PCR/Fluorescence microscope	12.31	[42]
基因枪法 Particle bombardment	Tx430	幼胚 Immature embryo	pBSV003	nptⅡ	ubi1	25-35 mg·L^-1遗传霉素 Geneticin（G418）	GUS	PCR/GUS assay	27.20-46.60	[23]
基因枪法 Particle bombardment	IS4698	成熟胚 Mature embryo	pMD18T-UBI-NPTII/pMD18T-UBI-GUS	nptⅡ	ubi1	30 mg·L^-1遗传霉素 Geneticin（G418）	GUS	PCR/GUS assay	13.33	[43]
农杆菌介导法 Agrobacterium-mediated transformation LBA4404	P898012/ PHI391	幼胚 Immature embryo	pSB1/pSB11	bar	ubi1	5 mg·L^-1 PPT	GUS	Southern blot	2.10	[8]
农杆菌介导法 Agrobacterium-mediated transformation EHA105/EHA101/AGL1	Tx430/C401/ Wheatland	幼胚/叶子 Immature embryo/Leaf	pPZP200/ pUC18	-	ubi1/CaMV35S/ HBT/act1/adh1	-	GUS/GFP	荧光显微镜/GUS assay Fluorescence microscope/ GUS assay	-	[5]
农杆菌介导法 Agrobacterium-mediated transformation LBA4404	P898012	幼胚 Immature embryo	pTOK233	hpt	CaMV35S	15 mg·L^-1潮霉素 Hygromycin	GUS	Southern blot/GUS assay	0.80-3.50	[20]
农杆菌介导法 Agrobacterium-mediated transformation EHA101	C401/ Pioneer8505	幼胚 Immature embryo	pPZP201	pmi	ubi1	1%-2%甘露糖 Mannose	GFP	Southern blot/Western blot/荧光显微镜 Southern blot/Western blot/ Fluorescence microscope	2.88-3.30	[44]
农杆菌介导法 Agrobacterium-mediated transformation EHA101	Tx430/C401/ Pioneer8505	幼胚 Immature embryo	pPZP201	-	ubi1	-	GFP	Southern blot/Western blot/荧光显微镜 Southern blot/Western blot/ Fluorescence microscope	1.00-3.00	[45]
农杆菌介导法 Agrobacterium-mediated transformation NTL4	Tx430/C2-97	幼胚 Immature embryo	pPTN290	nptⅡ	ubi1	10 mg·L^-1遗传霉素 Geneticin/20 mg·L^-1 巴龙霉素Paromomycin	GUS	Southern blot/GUS assay	0.30-4.50	[21]
农杆菌介导法 Agrobacterium-mediated transformation LBA4404	Sensako 85/ 1191	幼胚 Immature embryo	pCAMBIA1301	hpt	CaMV35S	5 mg·L^-1潮霉素 Hygromycin	GUS	Southern blot/GUS assay	5.00	[25]
农杆菌介导法 Agrobacterium-mediated transformation EHA101/LBA4404	P898012/Tx430/296B/C401	幼胚 Immature embryo	pPZP201	pmi	ubi1	1%-2%甘露糖 Mannose	GFP	PCR/Western blot/荧光显微镜 PCR/Western blot/ Fluorescence microscope	8.30	[46]
农杆菌介导法 Agrobacterium-mediated transformation EHA105	恢复系 Restorer line 115/5-27/9198/R011 保持系 Maintainer line ICS21B/21B	幼穗 Immature infloresences	pKUB	hpt	CaMV35S/ ubi1	50 mg·L^-1潮霉素 Hygromycin	GUS	RT-PCR/Southern blot/ Western blot	1.90	[10]
农杆菌介导法 Agrobacterium-mediated transformation EHA101	P898012	幼胚 Immature embryo	pZY102/pZY101 -TC2/pZY101-SKRS	bar	CaMV35S/ CZ19B1	2.5 mg·L^-1草铵膦 Glufosinate-ammonium	-	PCR/Southern blot	0.40-0.70	[47]
农杆菌介导法 Agrobacterium-mediated transformation LBA4404/EHA105	CO25/ TNS586	幼胚 Immature embryo	pMKURF2/ pCAMBIAG11/ pCAMBIARC7	bar/hpt	ubi1	1-2 mg·L^-1 bialophos/ 10-25 mg·L^-1潮霉素 Hygromycin	GUS	Western blot	1.60-2.70	[48]
农杆菌介导法 Agrobacterium-mediated transformation LBA4404	M 35-1	成熟胚/幼苗/叶基/幼穗 Mature embryo/Young seedling/Leaf base/ Immature infloresences	pKU352NA	hpt	ubi1	20 mg·L^-1潮霉素 Hygromycin	SGFPS65T	Inverse PCR	4.28	[17]
农杆菌介导法 Agrobacterium-mediated transformation NTL4	P898012/ RTX430	幼胚 Immature embryo	pCAMBIA1305.2/pCAM-Ubi-gus	hpt	ubi1	20 mg·L^-1潮霉素 Hygromycin	GUS	PCR/Southern blot	2.40	[24]
农杆菌介导法 Agrobacterium-mediated transformation LBA4404	APK1	芽尖 Shoot tip	pCAMBIA1305.1	hpt	CaMV35S	5 mg·L^-1潮霉素 Hygromycin	GUS	PCR/Southern blot	1.20-3.90	[49]
农杆菌介导法 Agrobacterium-mediated transformation LBA4404/AGL1	TX430	幼胚 Immature embryo	pSB1/pSB11	moPAT/ pmi	ubi1	5-10 mg·L^-1 PPT/ 12.5 g·L^-1 甘露糖Mannose	DSRED	qPCR	10.00-33.00	[22]
农杆菌介导法 Agrobacterium-mediated transformation AGL1/EHA101/GV3101	P898012	幼胚 Immature embryo	pZY102/ pFGC5941/ pFGC161	bar	MAS/ubi1/ CaMV35S	2.5 mg·L^-1草铵膦 Glufosinate-ammonium	GUS	PCR/Southern blot	14.00	[50]
农杆菌介导法 Agrobacterium-mediated transformation EHA105	U68/Tx623B	成熟胚 Mature embryo	pCAMBIA1305.1 /pCUbi1390	hpt	ubi1	-	GFP	PCR/Western blot	4.00	[29]
农杆菌介导法 Agrobacterium-mediated transformation EHA105	P898012	幼胚 Immature embryo	PHP78891	-	ubi1	-	GFP	PCR/Southern blot	6.20	[14]
农杆菌介导法 Agrobacterium-mediated transformation LBA4404 Thy-	Tx430/Macia/ Malisor84-7/ Tegemeo	幼胚 Immature embryo	pPHP38332/ pPHP78152/ pPHP78233/ pPHP81561/ pPHP70444	pmi/nptⅡ/ PTXD	ubi1	12.5 g·L^-1甘露糖 Mannose/250 mg·L^-1 遗传霉素Geneticin (G418)/300 mg·L^-1 Phi	YFP	qPCR	6.00-29.00	[13]
农杆菌介导法 Agrobacterium-mediated transformation GV2260	Tx430	幼胚 Immature embryo	pEKH2	hpt	CaMV35S	15 mg·L^-1潮霉素 Hygromycin	GUS	PCR/Southern blot	1.90	[51]
农杆菌介导法 Agrobacterium-mediated transformation AGL1/EHA101	P898012/ Btx623	幼胚 Immature embryo	PHP78891	-	ubi1/Rab17/ Nos	-	GFP	荧光显微镜 Fluorescence microscope	-	[15]
农杆菌介导法 Agrobacterium-mediated transformation EHA105	BTx623	幼胚 Immature embryo	pMDC32- 35S-GFP	hpt	CaMV35S	2-5 mg·L^-1潮霉素 Hygromycin	GFP	PCR/RT-PCR/荧光显微镜 PCR/RT-PCR/Fluorescence microscope	0.73	[12]
农杆菌介导法 Agrobacterium-mediated transformation LBA4404	Tx430/ Tx2737	幼穗/芽尖 Immature infloresences/ Shoot tip	pBI121	nptⅡ	CaMV35S	50-100 mg·L^-1 卡那霉素Kanamycin	GUS	PCR/GUS assay	4.20-14.30	[11]
农杆菌介导法 Agrobacterium-mediated transformation LBA4404 Thy-	Tx430/Tx623/ Tx2752/Macia/ Malisor 84-7/ Tegemeo	幼胚 Immature embryo	pPHP79066/ pPHP81814	Hra/nptⅡ	Pltp/Axig1	250 mg·L^-1遗传霉素 Geneticin (G418)	ZS- YELLOW1	荧光显微镜 Fluorescence microscope	17.10-69.70	[16]
农杆菌介导法 Agrobacterium-mediated transformation LBA4404 TD Thy-	Tx430	叶片 Leaf	PHP96037	Hra	ubi1/Nos	-	-	-	35.00-37.00	[18]

Table 1

References 66

[1]	韩立杰, 才宏伟. 高粱粒重遗传研究进展. 生物技术通报, 2019, 35(5): 15-27. doi: 10.13560/j.cnki.biotech.bull.1985.2019-0105
	HAN L J, CAI H W. Progress on genetic research of sorghum grain weight. Biotechnology Bulletin, 2019, 35(5): 15-27. (in Chinese)
[2]	PATERSON A H, BOWERS J E, BRUGGMANN R, DUBCHAK I, GRIMWOOD J, GUNDLACH H, HABERER G, HELLSTEN U, MITROS T, POLIAKOV A, SCHMUTZ J, SPANNAGL M, TANG H B, WANG X Y, WICKER T, BHARTI A K, CHAPMAN J, FELTUS F A, GOWIK U, GRIGORIEV I V, LYONS E, MAHER C A, MARTIS M, NARECHANIA A, OTILLAR R P, PENNING B W, SALAMOV A A, WANG Y, ZHANG L F, CARPITA N C, FREELING M, GINGLE A R, HASH C T, KELLER B, KLEIN P, KRESOVICH S, MCCANN M C, MING R, PETERSON D G, RAHMAN M, WARE D, WESTHOFF P, MAYER K F X, MESSING J, ROKHSAR D S. The Sorghum bicolor genome and the diversification of grasses. Nature, 2009, 457(7229): 551-556. doi: 10.1038/nature07723
[3]	倪先林, 赵甘霖, 刘天朋, 张长伟, 陈国民, 胡炯凌, 丁国祥. 高粱重要抗性性状的基因定位研究进展. 福建农业学报, 2012, 27(6): 652-660.
	NI X L, ZHAO G L, LIU T P, ZHANG C W, CHEN G M, HU J L, DING G X. Advances in Sorghum resistance gene mapping. Fujian Journal of Agricultural Sciences, 2012, 27(6): 652-660. (in Chinese)
[4]	GIRIJASHANKAR V, SWATHISREE V. Genetic transformation of Sorghum bicolor. Physiology and Molecular Biology of Plants, 2009, 15(4): 287-302. doi: 10.1007/s12298-009-0033-7
[5]	JEOUNG J M, KRISHNAVENI S, MUTHUKRISHNAN S, TRICK H N, LIANG G H. Optimization of sorghum transformation parameters using genes for green fluorescent protein and β-glucuronidase as visual markers. Hereditas, 2002, 137(1): 20-28. doi: 10.1034/j.1601-5223.2002.1370104.x
[6]	LIU G Q, GODWIN I D. Highly efficient sorghum transformation. Plant Cell Reports, 2012, 31(6): 999-1007. doi: 10.1007/s00299-011-1218-4 pmid: 22234443
[7]	CASAS A M, KONONOWICZ A K, ZEHR U B, TOMES D T, AXTELL J D, BUTLER L G, BRESSAN R A, HASEGAWA P M. Transgenic sorghum plants via microprojectile bombardment. Proceedings of the National Academy of Sciences of the United States of America, 1993, 90(23): 11212-11216.
[8]	ZHAO Z Y, CAI T S, TAGLIANI L, MILLER M, WANG N, PANG H, RUDERT M, SCHROEDER S, HONDRED D, SELTZER J, PIERCE D. Agrobacterium-mediated sorghum transformation. Plant Molecular Biology, 2000, 44(6): 789-798. doi: 10.1023/a:1026507517182 pmid: 11202440
[9]	ABLE J A, RATHUS C, GODWIN I D. The investigation of optimal bombardment parameters for transient and stable transgene expression in Sorghum. In Vitro Cellular & Developmental Biology Plant, 2001, 37(3): 341-348.
[10]	张明洲, 唐乔, 陈宗伦, 刘军, 崔海瑞, 舒庆尧, 夏英武, ALTOSAAR I. 农杆菌介导Bt基因遗传转化高粱. 生物工程学报, 2009, 25(3): 418-423.
	ZHANG M Z, TANG Q, CHEN Z L, LIU J, CUI H R, SHU Q Y, XIA Y W, ALTOSAAR I. Genetic transformation of Bt gene into Sorghum (Sorghum bicolor L.) mediated by Agrobacterium tumefaciens. Chinese Journal of Biotechnology, 2009, 25(3): 418-423. (in Chinese)
[11]	CHOU J, HUANG J, HUANG Y H. Simple and efficient genetic transformation of sorghum using immature inflorescences. Acta Physiologiae Plantarum, 2020, 42(3): 1-8. doi: 10.1007/s11738-019-2990-y
[12]	KURIYAMA T, SHIMADA S, MATSUI M. Improvement of Agrobacterium-mediated transformation for tannin-producing sorghum. Plant Biotechnology, 2019, 36(1): 43-48. doi: 10.5511/plantbiotechnology.19.0131a
[13]	CHE P, ANAND A, WU E, SANDER J D, SIMON M K, ZHU W W, SIGMUND A L, ZASTROW-HAYES G, MILLER M, LIU D L, LAWIT S J, ZHAO Z Y, ALBERTSEN M C, JONES T J. Developing a flexible, high-efficiency Agrobacterium-mediated sorghum transformation system with broad application. Plant Biotechnology Journal, 2018, 16(7): 1388-1395. doi: 10.1111/pbi.2018.16.issue-7
[14]	MOOKKAN M, NELSON-VASILCHIK K, HAGUE J, ZHANG Z J, KAUSCH A P. Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012 mediated by morphogenic regulators BABY BOOM and WUSCHEL2. Plant Cell Reports, 2017, 36(9): 1477-1491. doi: 10.1007/s00299-017-2169-1
[15]	NELSON-VASILCHIK K, HAGUE J, MOOKKAN M, ZHANG Z J, KAUSCH A. Transformation of recalcitrant Sorghum varieties facilitated by baby boom and Wuschel2. Current Protocols in Plant Biology, 2018, 3(4): e20076. doi: 10.1002/cppb.v3.4
[16]	CHE P, WU E, SIMON M K, ANAND A, LOWE K, GAO H R, SIGMUND A L, YANG M Z, ALBERTSEN M C, GORDON-KAMM W, JONES T J. Wuschel2 enables highly efficient CRISPR/Cas- targeted genome editing during rapid de novo shoot regeneration in sorghum. Communications Biology, 2022, 5: 344. doi: 10.1038/s42003-022-03308-w
[17]	JAMBAGI S, BHAT R, BHAT S, KURUVINASHETTI M S. Agrobacterium-mediated transformation studies in sorghum using an improved gfp reporter gene. Journal of SAT Agricultural Research, 2010, 8: 9.
[18]	WANG N, RYAN L, SARDESAI N, WU E, LENDERTS B, LOWE K, CHE P, ANAND A, WORDEN A, VAN DYK D, BARONE P, SVITASHEV S, JONES T, GORDON-KAMM W. Leaf transformation for efficient random integration and targeted genome modification in maize and sorghum. Nature Plants, 2023, 9(2): 255-270. doi: 10.1038/s41477-022-01338-0 pmid: 36759580
[19]	石太渊. 高粱组织培养研究进展. 杂粮作物, 2003, 23(6): 340-343.
	SHI T Y. Research progress of Sorghum tissue culture. Rain Fed Crops, 2003, 23(6): 340-343. (in Chinese)
[20]	CARVALHO C H S, ZEHR U B, GUNARATNA N, ANDERSON J, KONONOWICZ H H, HODGES T K, AXTELL J D. Agrobacterium-mediated transformation of sorghum: Factors that affect transformation efficiency. Genetics and Molecular Biology, 2004, 27(2): 259-269. doi: 10.1590/S1415-47572004000200022
[21]	HOWE A, SATO S, DWEIKAT I, FROMM M, CLEMENTE T. Rapid and reproducible Agrobacterium-mediated transformation of sorghum. Plant Cell Reports, 2006, 25(8): 784-791. doi: 10.1007/s00299-005-0081-6
[22]	WU E, LENDERTS B, GLASSMAN K, BEREZOWSKA-KANIEWSKA M, CHRISTENSEN H, ASMUS T, ZHEN S F, CHU U, CHO M J, ZHAO Z Y. Optimized Agrobacterium-mediated sorghum transformation protocol and molecular data of transgenic sorghum plants. In Vitro Cellular & Developmental Biology-Plant, 2014, 50(1): 9-18.
[23]	BELIDE S, VANHERCKE T, PETRIE J R, SINGH S P. Robust genetic transformation of Sorghum (Sorghum bicolor L.) using differentiating embryogenic callus induced from immature embryos. Plant Methods, 2017, 13: 109. doi: 10.1186/s13007-017-0260-9
[24]	KUMAR V, CAMPBELL L M, RATHORE K S. Rapid recovery- and characterization of transformants following Agrobacterium-mediated T-DNA transfer to sorghum. Plant Cell, Tissue and Organ Culture, 2011, 104(2): 137-146. doi: 10.1007/s11240-010-9809-2
[25]	NGUYEN T V, THU T T, CLAEYS M, ANGENON G. Agrobacterium-mediated transformation of Sorghum (Sorghum bicolor (L.) Moench) using an improved in vitro regeneration system. Plant Cell, Tissue and Organ Culture, 2007, 91(2): 155-164. doi: 10.1007/s11240-007-9228-1
[26]	刘宣雨, 刘树君, 宋松泉. 建立甜高粱(Sorghum bicolor)高频、高效再生体系的研究. 中国农业科学, 2010, 43(23): 4963-4969. doi: 10.3864/j.issn.0578-1752.2010.23.023.
	LIU X Y, LIU S J, SONG S Q. Research on establishment of a highly frequent and efficient regeneration system of Sorghum bicolor. Scientia Agricultura Sinica, 2010, 43(23): 4963-4969. doi: 10.3864/j.issn.0578-1752.2010.23.023. (in Chinese)
[27]	SAI N K, VISARADA K, LAKSHMI Y A, PASHUPATINATH E, RAO S V, SEETHARAMA N. In vitro culture methods in sorghum with shoot tip as the explant material. Plant Cell Reports, 2006, 25(3): 174-182. doi: 10.1007/s00299-005-0044-y
[28]	GIRIJASHANKAR V, SHARMA H C, SHARMA K K, SWATHISREE V, PRASAD L S, BHAT B V, ROYER M, SECUNDO B S, NARASU M L, ALTOSAAR I, SEETHARAMA N. Development of transgenic sorghum for insect resistance against the spotted stem borer (Chilo partellus). Plant Cell Reports, 2005, 24(9): 513-522. doi: 10.1007/s00299-005-0947-7 pmid: 16172896
[29]	LI J Q, WANG L H, ZHAN Q W, FAN F F, ZHAO T, WAN H B. Development of a simple and efficient method for Agrobacterium- mediated transformation in Sorghum. International Journal of Agriculture and Biology, 2015, 18(1): 134-138. doi: 10.17957/IJAB
[30]	OU-LEE T M, TURGEON R, WU R. Expression of a foreign gene linked to either a plant-virus or a Drosophila promoter, after electroporation of protoplasts of rice, wheat, and sorghum. Proceedings of the National Academy of Sciences of the United States of America, 1986, 83(18): 6815-6819.
[31]	BATTRAW M, HALL T C. Stable transformation of Sorghum bicolor protoplasts with chimeric neomycin phosphotransferase Ⅱ and β-glucuronidase genes. Theoretical and Applied Genetics, 1991, 82(2): 161-168. doi: 10.1007/BF00226207
[32]	HAGIO T, BLOWERS A D, EARLE E D. Stable transformation of sorghum cell cultures after bombardment with DNA-coated microprojectiles. Plant Cell Reports, 1991, 10(5): 260-264. doi: 10.1007/BF00232571 pmid: 24221592
[33]	石太渊, 杨立国, 王颖, 郑文静, 高连军. PHY157广谱抗病基因导入高粱及转基因植株的筛选与研究. 杂粮作物, 2001, 21(1): 12-14.
	SHI T Y, YANG L G, WANG Y, ZHENG W J, GAO L J. Transfering broad-spectrum resistant PYH157 gene into sorghum and screening transgenic plants. Rain Fed Crops, 2001, 21(1): 12-14. (in Chinese)
[34]	WANG W Q, WANG J X, YANG C P, LI Y H, LIU L, XU J. Pollen-mediated transformation of Sorghum bicolor plants. Biotechnology and Applied Biochemistry, 2007, 48(Pt2): 79-83. doi: 10.1042/BA20060231
[35]	CASAS A M, KONONOWICZ A K, HAAN T G, ZHANG L Y, TOMES D T, BRESSAN R A, HASEGAWA P M. Transgenic sorghum plants obtained after microprojectile bombardment of immature inflorescences. In Vitro Cellular & Developmental Biology-Plant, 1997, 33(2): 92-100.
[36]	ZHU H, MUTHRUKRISHNAN S, KRISHNAVENI S, WILDE G, JEOUNG J M, LIANG G H. Biolistic transformation of sorghum using a rice chitinase gene. Journal of Genetics and Breeding, 1998, 52: 243-252.
[37]	EMANI C, SUNILKUMAR G, RATHORE K S. Transgene silencing and reactivation in sorghum. Plant Science, 2002, 162(2): 181-192. doi: 10.1016/S0168-9452(01)00559-3
[38]	石太渊, 杨立国, 肖军. 基因枪轰击高粱愈伤组织获得转基因植株. 辽宁农业科学, 2003(6): 9-10.
	SHI T Y, YANG L G, XIAO J. Transgenic plant were obtained by bombardment sorghum callus with gene gun. Liaoning Agricultural Science, 2003(6): 9-10. (in Chinese)
[39]	TADESSE Y, SÁGI L, SWENNEN R, JACOBS M. Optimisation of transformation conditions and production of transgenic Sorghum (Sorghum bicolor) via microparticle bombardment. Plant Cell, Tissue and Organ Culture, 2003, 75(1): 1-18. doi: 10.1023/A:1024664817800
[40]	RAGHUWANSHI A, BIRCH R G. Genetic transformation of sweet sorghum. Plant Cell Reports, 2010, 29(9): 997-1005. doi: 10.1007/s00299-010-0885-x pmid: 20535472
[41]	GROOTBOOM A W, MKHONZA N L, O`KENNEDY M M, CHAKAUYA E, KUNERT K, CHIKWAMBA R K. Biolistic mediated Sorghum (Sorghum bicolor L. Moench) transformation via mannose and bialaphos based selection systems. International Journal of Botany, 2010, 6(2): 89-94. doi: 10.3923/ijb.2010.89.94
[42]	韩立杰. 高粱粒重的QTL分析及qGW1的精细定位[D]. 北京: 中国农业大学, 2016.
	HAN L J. QTL analysis of grain weight and fine mapping of qGW1in sorghum (Sorghum bicolor L.)[D]. Beijing: China Agricultural University, 2016. (in Chinese)
[43]	WANG L H, GAO L, LIU G Q, MENG R R, LIU Y L, LI J Q. An efficient sorghum transformation system using embryogenic calli derived from mature seeds. PeerJ, 2021, 9: e11849. doi: 10.7717/peerj.11849
[44]	GAO Z S, JAYARAJ J, MUTHUKRISHNAN S, CLAFLIN L, LIANG G H. Efficient genetic transformation of Sorghum using a visual screening marker. Genome, 2005, 48(2): 321-333. doi: 10.1139/g04-095
[45]	GAO Z S, XIE X J, LING Y, MUTHUKRISHNAN S, LIANG G H. Agrobacterium tumefaciens-mediated sorghum transformation using a mannose selection system. Plant Biotechnology Journal, 2005, 3(6): 591-599.
[46]	GUREL S, GUREL E, KAUR R, WONG J, MENG L, TAN H Q, LEMAUX P G. Efficient, reproducible Agrobacterium-mediated transformation of sorghum using heat treatment of immature embryos. Plant Cell Reports, 2009, 28(3): 429-444. doi: 10.1007/s00299-008-0655-1
[47]	LU L, WU X R, YIN X Y, MORRAND J, CHEN X L, FOLK W R, ZHANG Z J. Development of marker-free transgenic Sorghum (Sorghum bicolor (L.) Moench) using standard binary vectors with bar as a selectable marker. Plant Cell, Tissue and Organ Culture, 2009, 99(1): 97-108. doi: 10.1007/s11240-009-9580-4
[48]	INDRAARULSELVI P, MICHAEL P, UMAMAHESWARI S, KRISHNAVENI S. Agrobacterium mediated transformation of Sorghum bicolor for disease resistance. International Journal of Pharma and Bio Sciences, 2010, 1: 272-281.
[49]	IGNACIMUTHU S, PREMKUMAR A. Development of transgenic Sorghum bicolor (L.) Moench resistant to the Chilo partellus (Swinhoe) through Agrobacterium-mediated transformation. Molecular Biology and Genetic Engineering, 2014, 2(1): 1-8. doi: 10.7243/2053-5767-2-1
[50]	DO P T, LEE H, MOOKKAN M, FOLK W R, ZHANG Z J. Rapid and efficient Agrobacterium-mediated transformation of Sorghum (Sorghum bicolor) employing standard binary vectors and bar gene as a selectable marker. Plant Cell Reports, 2016, 35(10): 2065-2076. doi: 10.1007/s00299-016-2019-6
[51]	SATO-IZAWA K, TOKUE K, EZURA H. Development of a stable Agrobacterium-mediated transformation protocol for Sorghum bicolor Tx430. Plant Biotechnology, 2018, 35(2): 181-185. doi: 10.5511/plantbiotechnology.18.0424a
[52]	刘宣雨, 王青云, 刘树君, 宋松泉. 高粱遗传转化研究进展. 植物学报, 2011, 46(2): 216-223. doi: 10.3724/SP.J.1259.2011.00216
	LIU X Y, WANG Q Y, LIU S J, SONG S Q. Advances in the genetic transformation of Sorghum bicolor. Chinese Bulletin of Botany, 2011, 46(2): 216-223. (in Chinese) doi: 10.3724/SP.J.1259.2011.00216
[53]	SCHNIPPENKOETTER W, LO C, LIU G Q, DIBLEY K, CHAN W L, WHITE J, MILNE R, ZWART A, KWONG E, KELLER B, GODWIN I, KRATTINGER S G, LAGUDAH E. The wheat Lr34 multipathogen resistance gene confers resistance to anthracnose and rust in sorghum. Plant Biotechnology Journal, 2017, 15(11): 1387-1396. doi: 10.1111/pbi.12723 pmid: 28301718
[54]	MAHESWARI M, VARALAXMI Y, VIJAYALAKSHMI A, YADAV S K, SHARMILA P, VENKATESWARLU B, VANAJA M, SARADHI P P. Metabolic engineering using mtlD gene enhances tolerance to water deficit and salinity in sorghum. Biologia Plantarum, 2010, 54(4): 647-652. doi: 10.1007/s10535-010-0115-y
[55]	ZHANG H L, YU F F, XIE P, SUN S Y, QIAO X H, TANG S Y, CHEN C X, YANG S, MEI C, YANG D K, WU Y R, XIA R, LI X, LU J, LIU Y X, XIE X W, MA D M, XU X, LIANG Z W, FENG Z H, HUANG X H, YU H, LIU G F, WANG Y C, LI J Y, ZHANG Q F, CHEN C, OUYANG Y D, XIE Q. A Gγ protein regulates alkaline sensitivity in crops. Science, 2023, 379(6638): eade8416. doi: 10.1126/science.ade8416
[56]	ZHAO Z, TOMES D. Sorghum Transformation. Genetic Transformation of Plants. Berlin Heidelberg: Springer Verlag Press, 2003: 91-107.
[57]	JIANG W Z, ZHOU H B, BI H H, FROMM M, YANG B, WEEKS D P. Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice. Nucleic Acids Research, 2013, 41(20): e188. doi: 10.1093/nar/gkt780
[58]	LI A X, JIA S G, YOBI A, GE Z X, SATO S J, ZHANG C, ANGELOVICI R, CLEMENTE T E, HOLDING D R. Editing of an alpha-kafirin gene family increases, digestibility and protein quality in Sorghum. Plant Physiology, 2018, 177(4): 1425-1438. doi: 10.1104/pp.18.00200
[59]	ZHANG D, TANG S Y, XIE P, YANG D K, WU Y R, CHENG S J, DU K, XIN P Y, CHU J F, YU F F, XIE Q. Creation of fragrant sorghum by CRISPR/Cas9. Journal of Integrative Plant Biology, 2022, 64(5): 961-964. doi: 10.1111/jipb.13232
[60]	TESSO T, KAPRAN I, GRENIER C, SNOW A, SWEENEY P, PEDERSEN J, MARX D, BOTHMA G, EJETA G. The potential for crop-to-wild gene flow in Sorghum in Ethiopia and Niger: A geographic survey. Crop Science, 2008, 48(4): 1425-1431. doi: 10.2135/cropsci2007.08.0441
[61]	SILVA T N, THOMAS J B, DAHLBERG J, RHEE S Y, MORTIMER J C. Progress and challenges in sorghum biotechnology, a multipurpose feedstock for the bioeconomy. Journal of Experimental Botany, 2022, 73(3): 646-664. doi: 10.1093/jxb/erab450
[62]	HAN Y, BROUGHTON S, LIU L, ZHANG X Q, ZENG J B, HE X Y, LI C D. Highly efficient and genotype-independent barley gene editing based on anther culture. Plant Communications, 2021, 2(2): 100082. doi: 10.1016/j.xplc.2020.100082
[63]	DEY M, BAKSHI S, GALIBA G, SAHOO L, PANDA S K. shoot apex in rice (Oryza sativa spp. indica) using TDZ. 3 Biotech, 2012, 2(3): 233-240. doi: 10.1007/s13205-012-0051-y
[64]	MA Z Y, LIU J F, WANG X F. Agrobacterium-mediated transformation of cotton (Gossypium hirsutum) shoot apex with a fungal phytase gene improves phosphorus acquisition. Methods in Molecular Biology, 2013, 958: 211-222. doi: 10.1007/978-1-62703-212-4_18 pmid: 23143496
[65]	CAO X S, XIE H T, SONG M L, LU J H, MA P, HUANG B Y, WANG M G, TIAN Y F, CHEN F, PENG J, LANG Z B, LI G F, ZHU J K. Cut-dip-budding delivery system enables genetic modifications in plants without tissue culture. The Innovation, 2023, 4(1): 100345. doi: 10.1016/j.xinn.2022.100345
[66]	DEMIRER G S, ZHANG H, MATOS J L, GOH N S, CUNNINGHAM F J, SUNG Y, CHANG R, ADITHAM A J, CHIO L, CHO M J, STASKAWICZ B, LANDRY M P. High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants. Nature Nanotechnology, 2019, 14(5): 456-464. doi: 10.1038/s41565-019-0382-5 pmid: 30804481

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