豇豆属食用豆类间的远缘杂交

doi:10.3864/j.issn.0578-1752.2015.03.03

Abstract

Abstract: 【Objective】The aim of this study was to analyze the crossability of different Vigna species, which will facilitate the distant hybridization breeding of Vigna. 【Method】 Interspecific crossabilities were investigated among 5 cultivar species of Vigna, V. radiata (mungbean), V. mungo (black gram), V. umbellata (rice bean), V. anguluris (adzuki bean), V. unguiculata (cowpea), and 3 wild relative species of adzuki bean, V. minima, V. nakashiame and V. riukiuensis. Embryo rescue was conducted for 3 interspecific combinations, namely, V. radiata×V. angularis, V. angularis×V. umbellata and V. umbellata×V. angularis, respectively. The pod setting ratio of hybrid and performance of F₁ plant were investigated to analyze the crossabilities between food legumes of Vigna genus. 【Result】 The genetic relationship between different food legumes and the choice of female parent influenced the result of distant hybridization. Except for cowpea cross combinations, hybrid pods were set successfully in the other interspecific crosses. The growth of hybrid pods and seeds showed variations in different combinations. No genetic disorders before fertilization were found from the hybridizations of V. radiata×V. mungo, V. angularis×V. minima, V. angularis×V. nakashiame, and V. angularis×V. riukiuensis. The hybrid seeds of them germinated normally, and the hybrid plants were fertile, but the fruiting rates were lower. The pod setting ratio was significantly higher when V. radiata was selected as female parent in the interspecific crosses between V. radiata and V. umbellata. F₁ plants were obtained without embryo culture, but they showed complete hybrid sterility. No fertile seeds were obtained even backcross to V. radiata or V. umbellata. Hybrid plants were obtained from cultured embryos for the interspecific crosses of V. radiata ×V. angularis and V. umbellata ×V. angularis. The fertility of F₁ plants from these two combinations was opposite, sterile in the former and fertile in the latter. The hybrid pods shriveled or fell off, or embryo abortion occurred in all other interspecific crosses. 【Conclusion】Using mungbean as the female parent was of benefit to the development of the hybrid pods and seeds when crossed with blackgram, adzuki bean and rice bean, respectively. The embryo abortion occurred when crossed blackgram with adzuki bean and rice bean, respectively. Through the use of embryo culture, the fertile F₁ plants could be obtained when using rice bean as the female parent crossed with adzuki bean. The crossability of adzuki bean with its wild relative was better than that of rice bean. All the crosses were failure when using cowpea as one of the parents.

Key words: Vigna genus, food legumes, interspecific hybridization, embryo rescue

LIU Chang-you, FAN Bao-jie, CAO Zhi-min, SU Qiu-zhu, WANG Yan, ZHANG Zhi-xiao, CHENG Xu-zhen, TIAN Jing. Interspecific Hybridization Among Vigna Species[J].Scientia Agricultura Sinica, 2015, 48(3): 426-435.

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References

[1] 董玉琛. 小麦远缘杂交育种//中国农学会主编. 21世纪小麦遗传育种展望—小麦遗传育种国际学术讨论会文集. 北京: 中国农业科技出版社, 2001: 12-16.

Dong Y C. Distant hybridization breeding of wheat//Chinese Association of Agricultural Science Societies, eds. Prospect of Wheat Genetics and Breeding in The 21^st Century—Paper Collection of International Wheat Genetics and Breeding Conferences. Beijing: China Science and Technology Press, 2001: 12-16. (in Chinese)

[2] 安调过, 许红星, 许云峰. 小麦远缘杂交种质资源创新. 国生态农业学报, 2011, 19(5): 1011-1019.

An D G, Xu H X, Xu Y F. Enhancement of wheat distant hybridization germplasm. Chinese Journal of Eco-Agriculture, 2011, 19(5): 1011-1019. (in Chinese)

[3] 祁适雨, 于世选, 张耀辉, 于光华, 宋凤英. 春小麦与天蓝冰草远缘杂交育种的研究. 中国农业科学, 1979, 12(2): 1-11.

Qi S Y, Yu S X, Zhang Y H, Yu G H, Song F Y. Studies on wheat breeding by distant hybridization between wheat and Agropyron glaucum. Scientia Agricultura Sinica,1979, 12(2): 1-11. (in Chinese)

[4] 郑卓杰. 中国食用豆类学. 北京: 中国农业出版社, 1997: 3-6.

Zheng Z J. Food Legumes in China. Beijing: Chinese Agricultural Press, 1997: 3-6. (in Chinese)

[5] Tomooka N, Vaughan D A, Moss H. The Asian Vigna: Genus Vigna Subgenus Ceratotropis Genetic Resources. London: Kluwer Academic Publishers, 2002: 92-184.

[6] Devi L S, Gupta P. Evaluation of greengram and blackgram varieties against cyst nematode-Heterodera cajani. Indian Journal of Nematology,1987, 17(2): 341.

[7] Tomooka N, Kashiwaba K, Vaughan D A, Ishimoto M, Egawa Y. The effectiveness of evaluating wild species: Searching for sources of resistance to bruchid beetles in the genus Vigna subgenus Ceratotropis. Euphytica, 2000, 115(1): 27-41.

[8] Kashiwaba K, Tomooka N, Kaga A, Han O K, Vaughan D A. Characterization of resistance to three bruchid species (Callosobruchus spp., Coleoptera, Bruchidae) in cultivated rice bean (Vigna umbellata).Journal of Economic Entomology, 2003, 96(1): 207-213.

[9] Fujii K, Miyazaki S. Infestation resistance of wild legumes (Vigna sublobata) to azuki bean weevil, Callosobruchus chinensis (L.) (Coleoptera: Bruchidae) and its relationship with cytogenetic classification. Applied Entomology and Zoology, 1987, 22: 229-230.

[10] Fujii K, Ishimoto M, Kitamura K. Patterns of resistance to bean weevils (Bruchidae) in Vigna radiata-mungo-sublobata complex inform the breeding of new resistant varieties. Applied Entomology and Zoology, 1989, 24: 126-132.

[11] Satoshi A, Kushida A. Breeding soybean cyst nematode resistant azuki bean using wild Vigna germplasm//14th MAFF International Workshop on Genetic Resources. Tsukuba: National Institute of Agrobiological Science, 2009: 103-108.

[12] Sen N K, Ghosh A K. Interspecific hybridization between Phaseolus aureus Roxb. (green gram) and Ph. mungo L. (black gram). Bulletin of the Botanical Society of Bengal, 1963, 14: 1-4.

[13] Dana S. The cross between Phaseolus aureus Roxb. and P. Mungo L.. Genetica, 1966, 37(1): 259-274.

[14] Gosal S S, Bajaj Y P S. Interspecific hybridization between Vigna mungo and Vigna radiata through embryo culture. Euphytica, 1983, 32: 129-137.

[15] Kalpana P, Raghuvanshi S S, Dhan P. Induced high yielding amphiploid of Vigna radiata × Vigna mungo. Cytologia, 1990, 55(2): 249-253.

[16] Ahn C S. Interspecific hybridization among four species of the genus Vigna savi[D]. Michigan: University of Hawaii, 1976.

[17] Ahn C S, Min K S, Chung S J, Chung S J, Koh G C, Park S H. Interspecific hybridization between Vigna angularis (Wild.) ohwi and ohashi and Vigna umbellata (Thunb.) ohwi and ohashi through in vitro culture of embryos. Kerean Journal of Breeding, 1986, 18(4): 283-291.

[18] Chen N C, Baker L R, Honma S. Interspecific crossability among four species of Vigna food legumes. Euphytica, 1983, 32: 925-937.

[19] Tanapon C, Prakit S, Peerasak S, Sontichai C, Rangsarid K, Surapong D. Development of tetraploid plants from an interspecific hybrid between mungbean (Vigna radiata) and rice bean (Vigna umbellata). Journal of Crop Science and Biotechnology, 2013, 16(1): 45-51.

[20] Siriwardhane D, Egawa Y, Tomooka N. Cross compatibility of cultivated adzuki bean (Vigna angularis) and rice bean (V. umbellata) with their wild relatives. Plant Breeding, 1991, 107: 320-325.

[21] 杨人俊, 韩亚光. 野赤豆在辽宁省的地理分布及其与赤豆间的杂交试验. 作物学报, 1994, 20(5): 607-613.

Yang R J, Han Y G. The geographical distribution of Vigna angularis var. nipponensis in Liaoning Prov. of China and artificial crossing between it and V. angularis var. angularis. Acta Agronomica Sinica, 1994, 20(5): 607-613. (in Chinese)

[22] 刘玉平, 田静, 和剑涵, 李辉. 关于绿豆杂交方法的探讨. 河北农业科学, 1997, 1(1): 9-11.

Liu Y P, Tian J, He J H, Li H. Study of mungbean hybridization method. Journal of Hebei Agricultural Sciences, 1997, 1(1): 9-11. (in Chinese)

[23] 程须珍, 王素华, 王丽侠. 绿豆种质资源描述规范和数据标准. 北京: 中国农业出版社, 2006: 13-43.

Cheng X Z, Wang S H, Wang L X. Descriptor and Data Standard for Mungbean [Vigna radiata (L.) Wilczek]. Beijing: China Agriculture Press, 2006: 13-43. (in Chinese)

[24] 邓衍明, 叶晓青, 佘建明, 汤日圣. 植物远缘杂交育种研究进展. 华北农学报, 2011, 26(增刊): 52-55.

Deng Y M, Ye X Q, She J M, Tang R S. The research progress on distant hybridization of plant breeding. Acta Agriculturae Boreali-Sinica, 2011, 26(Suppl.): 52-55. (in Chinese)

[25] 李振声. 创造新物种与改良旧物种-远缘杂交在作物育种中的应用. 中国农业科学, 1977, 3: 38-42.

Li Z S. Creating new species and improving the old species-the role of distant hybridization in crop breeding. Scientia Agricultura Sinica, 1977, 3: 38-42. (in Chinese)

[26] Kaga A, Tomooka N, Egawa Y, Hosaka K, Kamijima O. Species relationships in the subgenus Ceratotropis (Genus vigna) as revealed by RAPD analysis. Euphytica, 1996, 88: 17-24.

[27] 张天真. 作物育种学总论. 北京: 中国农业出版社, 2003: 114-228.

Zhang T Z. General Crop Breeding. Beijing: China Agriculture Press, 2003: 114-128. (in Chinese)

[28] Gutierrez-Marcos J F, Pennington P D, Costa L M, Dickinson H G. Imprinting in the endosperm: A possible role in preventing wide hybridization. Philosophical Transactions of the Royal Society Series B, 2003, 358: 1105-1111．

[29] 安调过, 钟冠昌, 李俊明, 王志国, 王彦梅, 纪军. 半浸根法加倍小麦远缘杂交幼胚再生植株染色体. 作物学报, 2003, 29(6): 955-957.

An D G, Zhong G C, Li J M, Wang Z G, Wang Y M, Ji J. Chromosome doubling methods of immature embryo plants of wheat distant hybridization. Acta Agronomica Sinica, 2003, 29(6): 955-957. (in Chinese)

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Interspecific Hybridization Among Vigna Species

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