Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (3): 415-425.doi: 10.3864/j.issn.0578-1752.2015.03.02

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Evolution of Genetic Distance Between Parental Lines of Chinese Sorghum Hybrids from1970s-2000s Based on SSR Analysis

WANG Rui1,2, WANG Jin-sheng1, ZHANG Fu-yao2, CHENG Qing-jun2, TIAN Cheng-hua2, LING Liang3   

  1. 1College of Life Sciences, Shanxi Agricultural University, Taigu 030801, Shanxi
    2Sorghum Institute, Shanxi Academy of Agricultural Sciences/ Key Laboratory of Genetic and Germplasm Innovation in sorghum for Shanxi Province, Yuci 030600, Shanxi
    3Institute of Edible Fungi, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, Shanxi
  • Received:2014-06-26 Online:2015-01-31 Published:2015-01-31

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Abstract: 【Objective】To explore the genetic differences of Chinese sorghum varieties and evolution of the superior groups since heterosis utilization, SSR markers were used to identify the genetic distance of sorghum parental lines and F1. Genetic background of Chinese sorghum main varieties in different periods were analyzed, and systematically understand of the genetic relationship, genetic diversity and genetic distance of sorghum hybrids and their parental lines can reduce the blindness of parental lines selection, effectively improve the predictability of cross breeding.【Method】SSR genetic distance of the representative hybrids and their parental lines grown in late-maturing area since 1970s were analyzed. Analysis of the superior groups of sorghum hybrids, research of the breeding progress and the evolution characteristics since heterosis utilization of sorghum were conducted at molecular level. 【Result】 A total 55 hybrids and their parental lines were evaluated by 109 pairs of SSR markers. The results revealed that 47 pairs of SSR markers were highly polymorphic. 373 varied alleles were amplified with an average of 7.5 alleles per marker, the ranges were 2 to 14. The ranges of PIC in 55 cultivars were 0.0351-0.8836, with an average of 0.6085. The genetic distance was averaged at 0.6011 with a range from 0.0889 to 0.9500. The sorghum materials were classified into 4 groups based on the SSR experiments. The clustering results were similar to those based on geographical origins and genetic backgrounds. The results showed that the average genetic distance between the two parental lines of hybrids from different eras showed a upward trend in 1970-1980, then a slight decline. According to the analysis of genetic distance between the two parental lines of hybrids from different heterosis models, Durra sterile lines×Kaoliang or trend Kaoliang restorer lines and Kafir-caudatum sterile lines×Kaoliang or trend kaoliang restorer lines have a far genetic distance, high yield, therefore, the heterosis model of Chinese sorghum hybrids should be Durra×kaoliang or trend kaoliang, Kafir-caudatum×kaoliang or trend kaoliang. 【Conclusion】In this study, it was found that the genetic distance between parental lines has a close relationship with heterosis level in sorghum, therefore, genetic distance should be fully considered in the matching of sorghum parental lines.

Key words: sorghum, SSR, genetic distance, heterosis

[1]    卢庆善, 孙毅. 杂交高粱遗传改良. 北京: 中国农业科学技术出版社, 2005.
Lu Q S, Sun Y. Genetic Improvement of Hybrid Sorghum. Beijing: China Agricultural Science and Technology Press, 2005. (in Chinese)
[2]    卢庆善. 我国高粱杂种优势利用回顾与展望. 辽宁农业科学, 1992, 3: 40-44.
Lu Q S. Retrospect and prospect on heterosis utilization of sorghum in China. Liaoning Agricultural Sciences, 1992, 3: 40-44. (in Chinese)
[3]    侯荷亭, 杜志宏, 赵根第. 高粱亲本遗传距离与杂种优势和特殊配合力的关系. 遗传, 1995, 17(1): 30-33.
Hou H T, Du Z H, Zhao G D. Studies on the relationships of genetic distance of sorghum parental lines with heterosis and specific combining ability. Hereditas, 1995, 17(1): 30-33. (in Chinese)
[4]    袁力行, 傅俊骅, 张世煌, 刘新芝, 彭泽斌, 李新海, Warburton M, Khairalla M. 利用RFLP和SSR标记划分玉米自交系杂种优势群的研究. 作物学报, 2001, 27(2): 149-156.
Yuan L X, Fu J H, Zhang S H, Liu X Z, Peng Z B, Li X H, Warburton M, Khairalla M. Heterotic grouping of maize inbred lines using RFLP and SSR markers. Acta Agronomica Sinica, 2001, 27(2): 149-156. (in Chinese)
[5]    李新海, 袁力行, 李晓晖, 张世煌, 李明顺, 李文华. 利用SSR标记划分70份我国玉米自交系的杂种优势群. 中国农业科学, 2003, 36(6): 622-627.
Li X H, Yuan L X, Li X H, Zhang S H, Li M S, Li W H. Heterotic grouping of 70 maize inbred lines by SSR markers. Scientia Agricultura Sinica, 2003, 36(6): 622-627. (in Chinese)
[6]    王亮, 周旭梅, 景希强, 张宝石, 李晓辉. 辽宁省不同年代玉米杂交种产量及杂种优势比较. 玉米科学, 2010, 18(3): 47-52.
Wang L, Zhou X M, Jing X Q, Zhang B S, Li X H. Comparisons of maize heterosis in different years in Liaoning Province. Journal of Maize Sciences, 2010, 18(3): 47-52. (in Chinese)
[7]    王江春, 胡延吉, 余松烈, 王振林, 刘爱峰, 王洪刚. 建国以来山东小麦品种及其亲本的亲缘系数分析. 中国农业科学, 2006, 39(4): 664-672.
Wang J C, Hu Y J, Yu S L, Wang Z L, Liu A F, Wang H G. Relationship coefficient analysis among winter wheat varieties and their parents in Shandong province after liberation. Scientia Agricultura Sinica, 2006, 39(4): 664-672. (in Chinese)
[8]    彭芹, 戴双, 郭骞欢, 程敦公, 李豪圣, 刘爱峰, 刘建军, 赵世杰, 宋健民. 1950年以来山东省主推小麦品种的遗传多样性演变. 分子植物育种, 2012, 10(2): 228-237.
Peng Q, Dai S, Guo Q H, Cheng D G, Li H S, Liu A F, Liu J J, Zhao S J, Song J M. The evolution of genetic diversity of wheat varieties released in Shandong province since 1950. Molecular Plant Breeding, 2012, 10(2): 228-237. (in Chinese)
[9]    罗小金, 贺浩华, 彭小松, 余秋英, 孙俊立, 张洪亮, 李自超. 利用SSR标记分析水稻亲本间遗传距离与杂种优势的关系. 植物遗传资源学报, 2006, 7(2): 209-214.
Luo X J, He H H, Peng X S, Yu Q Y, Sun J L, Zhang H L, Li Z C. Analysis on correlation between genetic distance of rice parents and heterosis with SSR molecular marker. Journal of Plant Genetic Resources, 2006, 7(2): 209-214. (in Chinese)
[10]   杨祥波, 刘晓丹, 刘广娜. SSR分子标记遗传距离预测大豆亲本杂种优势的研究. 安徽农业科学, 2011, 39(33): 20322-20324.
Yang X B, Liu X D, Liu G N. Research on the prediction of soybean parental heterosis with the genetic distance of SSR marker. Journal of Anhui Agricultural Sciences, 2011, 39(33): 20322-20324. (in Chinese)
[11]   Ali M L, Rajewski J F, Baenziger P S, Gill K S, Eskridge K M, Dweikat I. Assessment of genetic diversity and relationship among a collection of US sweet sorghum germplasm by SSR markers. Molecular Breeding, 2008, 21: 497-509.
[12]   赵香娜, 岳美琪, 刘洋, 顿宝庆, 赵伟华, 谭亮, 李桂英. 国内外甜高粱种质遗传多样性的SSR分析. 植物遗传资源学报, 2010, 11(4): 407-412.
Zhao X N, Yue M Q, Liu Y, Dun B Q, Zhao W H, Tan L, Li G Y. Genetic diversity of domestic and foreign sweet sorghum germplasm revealed by SSR markers. Journal of Plant Genetic Resources, 2010, 11(4): 407-412. (in Chinese)
[13]   Kong L, Dong J, Hart G E. Characteristics, linkage-map positions, and allelic differentiation of Sorghum bicolor (L.) Moench DNA simple-sequence repeats (SSRs). Theoretical and Applied Genetics, 2000, 101(3): 438-448.
[14]   Haussmann B I G, Hess D E, Seetharama N, Welz H G, Geiger H H. Construction of a combined sorghum linkage map from two recombinant inbred populations using AFLP, SSR, RFLP, and RAPD markers, and comparison with other sorghum maps. Theoretical and Applied Genetics, 2002, 105(4): 629-637.
[15]   Menz M A, Klein R R, Mullet J E, Obert J A, Unruh N C, Klein P E. A high-density genetic map of Sorghum bicolor (L.) Moench based on 2926 AFLP, RFLP and SSR markers. Plant Molecular Biology, 2002, 48(5/6): 483-499.
[16]   赵姝华, 李钥莹, 邹剑秋, Folkertsma R, Hash C T. 高粱分子遗传图谱的构建. 杂粮作物, 2005, 25(1):11-13.
Zhao S H, Li Y Y, Zou J Q, Folkertsma R, Hash C T. Construction of a molecular genetic map of sorghum(Sorghum bicolor L. Moench). Zaliang Zuowu (Rain Fed Crops), 2005, 25(1): 11-13 (in Chinese)
[17]   卞云龙, 邓德祥, 王益军, 才宏伟. 基于AFLP和SSR标记的高粱分子遗传连锁图谱构建. 分子植物育种, 2007, 5(5): 661-666.
Bian Y L, Deng D X, Wang Y J, Cai H W. Construction of genetic linkage map based on AFLP and SSR markers in sorghum. Molecular Plant Breeding, 2007, 5(5): 661-666. (in Chinese)
[18]   Burow G B, Franks C D, Acosta-Martinez V, Xin Z G. Molecular mapping and characterization of BLMC, a locus for profuse wax (bloom) and enhanced cuticular features of sorghum (Sorghum bicolor (L.) Moench.). Theoretical and Applied Genetics, 2009, 118: 423-431.
[19]   Mace E S, Rami J F, Bouchet S, Klein P E, Klein R R, Kilian A, Wenzl P, Xia L, Halloran K, Jordan D R. A consensus genetic map of sorghum that integrates multiple component maps and high- throughput Diversity Array Technology (DArT) markers. BMC Plant Biology, 2009, 9: 13.
[20]   Paterson A H, Bowers J E, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A,Schmutz J, Spannagl M, Tang H B, Wang X Y, Wicker T, BhartiA K, Chapman J, Feltus F A, Gowik U, Grigoriev I V, Lyons E, Maher C A, Martis M, Narechania A, Otillar R P, Penning B W, Salamov A A, Wang Y, Zhang L F, Carpita N C, Freeling M, Gingle A R, Hash C T, Keller B, Klein P, Kresovich S, McCann M C, Ming R, Peterson D G, Mehboobur R, Ware D, Westhoff P, Mayer K F X, Messing J, Rokhsar D S. The Sorghum bicolor genome and the diversification of grasses. Nature, 2009, 457: 551-556.
[21]   Satish K, Srinivas G, Madhusudhana R, Padmaja P G, Nagaraja Reddy R, Murali Mohan S, Seetharama N. Identification of quantitative trait loci for resistance to shoot fly insorghum [Sorghum bicolor (L.) Monech]. Theoretical and Applied Genetics, 2009, 119: 1425-1439.
[22]   Mace E S, Jordan D R. Location of major effect genes in sorghum (Sorghum bicolor (L.)Moench). Theoretical and Applied Genetics, 2010, 121: 1339-1356.
[23]   邹剑秋, 李玥莹, 朱凯, 王艳秋. 高粱丝黑穗病菌3号生理小种抗性遗传研究及抗病基因分子标记. 中国农业科学, 2010, 43(4): 713-720.
Zou J Q, Li Y Y, Zhu K, Wang Y Q. Study on inheritance and molecular makers of sorghum resistance to head smut physiological race 3. Scientia Agricultura Sinica, 2010, 43(4): 713-720. (in Chinese)
[24]   魏添梅, 昌小平, 闵东红, 景蕊莲. 小麦抗旱品种的遗传多样性分析及株高优异等位变异挖掘. 作物学报, 2010, 36(6): 895-904.
Wei T M, Chang X P, Min D H, Jing R L. Analysis of genetic  diversity and tapping elite alleles for plant height in drought-tolerant wheat varieties. Acta Agronomica Sinica, 2010, 36(6): 895-904. (in Chinese)
[25]   Liu K J, Muse S V. PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics, 2005, 21: 2128-2129.
[26]   Li Y, Li C Z. Genetic contribution of Chinese landraces to the development of sorghum hybrids. Euphytica, 1998, 102: 47-55.
[27]   王富德, 卢庆善. 我国主要高粱杂交种的系谱分析. 作物学报, 1985, 1(11): 9-14.
Wang F D, Lu Q S. An analysis of pedigrees of the main hybrid sorghums in China. Acta Agronomica Sinica, 1985, 1(11): 9-14. (in Chinese)
[28]   山西省忻县地区农业科学研究所. 杂交高粱—选育—制种—栽培. 北京: 科学出版社, 1972.
Xin County Institute of Agricultural Sciences in Shanxi Province. Hybrid Sorghum-Breeding-Seed Production-Cultivation. Beijing: Science Press, 1972. (in Chinese)
[29]   马鸿图. 高粱杂种优势与类型血缘关系的探讨. 沈阳农业大学学报, 1974, 1: 60-68.
Ma H T. The relation of heterosis in sorghum and consanguinity of type. Journal of Shenyang Agricultural Uniersity, 1974, 1: 60-68. (in Chinese)
[30]   李团银, 柳青山, 张福耀, 韦耀明. 新型A2细胞质高粱杂交种晋杂12号选育及利用研究. 中国农业科学, 1999, 32(1): 102-104
Li T Y, Liu Q S, Zhang F Y, Wei Y M. Development and commereial cultivation of A2 cytoplasmic sorghum hybrid Jinza 12. Scientia Agricultura Sinica, 1999, 32(1): 102-104. (in Chinese)
[31]   高士杰, 刘晓辉, 郭中校. 中国杂交高粱的种质基础及优势利用模式研究. 中国农学通报, 2005, 21(10): 106-109.
Gao S J, Liu X H, Guo Z X. Chinese hybrid sorghum idioplasm foundation and superiority use pattern. Chinese Agricultural Science, 2005, 21(10): 106-109. (in Chinese)
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