黑穗醋栗果实生长发育过程中抗坏血酸含量 及相关酶活性的变化

doi:10.3864/j.issn.0578-1752.2019.01.010

Abstract

Abstract:

【Objective】 The changes in the ascorbic acid (AsA) contents and the enzymatic activities during the anabolic process of different cultivars and growth stages of black currant fruits were studied to determine the relationship between AsA contents and anabolic enzymes during the growth and development of fruits, so as to provide a theoretical basis for comprehensively revealing the accumulation rule of AsA in black currant fruits. 【Method】 Three different cultivars of black currant fruits (Adelinia, Brodtrop and Heifeng) were studied and determined the contents of reduced AsA, oxidized ascorbic acid (DHA), reduced glutathione (GSH) and oxidized glutathione (GSSG) and anabolic enzymatic activities of young, expansion, half-veraison, veraison and maturity stages. 【Result】 There were significant diversities in fruit sizes, AsA contents and AsA metabolites of different cultivars of black currant fruits. The Adelinia had the largest weight of single fruit (1.97 g). During the growth and development process of fruits, the changes in the total ascorbic acid (T-AsA) and AsA contents of fruits were consistent among the three cultivars, and the young fruits had the highest contents. The AsA content of young Adelinia fruit was the highest (83.17 μmol?g ^-1 FW) and then sharply decreased rapidly to the maturity stage with the growth of the fruit, which decreased to 21.28 μmol?g ^-1 FW at maturity stage. The contents of GSH and T-GSH in the three cultivars increased with the development of fruits, but the different cultivars increased in different stages and degrees. The content of GSSG was quite different among different cultivars. For the mature fruits, the GSSG content of Heifeng was the lowest, which was 0.008 μmol?g ^-1 FW and only accounted for 10.2% of Adelinia. In AsA-GSH recycling regeneration metabolism, the activities of dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) showed the highest level at expansion period, and finally decreased to the lowest level at maturity stage. The DHAR and MDHAR activities of Brodtrop fruits showed slightly higher than those of Adelinia and Heifeng fruits. The activity of glutathione reductase (GR) was the highest level at young stage. The GR activity of Adelinia young fruits was the highest (0.06 μmol?min ^-1?g ^-1 FW), and then decreased with the growth of fruits. The changes in the activities of ascorbate peroxidase (APX) were similar to the changes in the activities of GR. The changes in the activities of L-galactose-1,4-lactone dehydrogenase (GalLDH), a key enzyme of L-galactose pathway, were consistent with the changes in AsA contents. The GalLDH activity of Adelinia young and mature fruits showed higher than that of Heifeng and Brodtrop young and mature fruits, respectively. According to the correlation analysis, the GalLDH activity showed a highly significant positive correlationship with T-AsA, AsA, DHA, DHAR and MDHAR. The correlation coefficient was above 0.91. The higher GalLDH activity was found in the fruits, the higher AsA contents of fruits also was found. There was a highly significant positive correlationship between DHAR and MDHAR, T-AsA and AsA. The APX had a high correlation with T-GSH and GSH. 【Conclusion】 The AsA content of black currant young fruits was the highest and there were significant differences among the three cultivars. The GalLDH, MDHAR and DHAR might be the key enzymes for AsA anabolism in black currant fruits. The accumulation of AsA content of black currant fruits resulted from the activity of GalLDH, which indicated that the anabolic pathway played a more important role and were found to be a dominant position. The related enzymes of AsA-GSH recycling regeneration pathway also contributed to the AsA anabolism. The accumulation of high AsA content in black currant fruits resulted from the combined effects of anabolic and recycling pathways.

Key words: Ribes nigrum L., fruit, ascorbic acid, anabolism, L-galactose-1, 4-lactone dehydrogenase

SUN XiaoJuan,LIU QingShuai,YUN AngRan,ZHANG Yan,HUO JunWei,QIN Dong,JIANG Ting. The Changes in the Contents of Ascorbic Acid and the Activities of Related Enzymes in Black Currant Fruits During the Process of Its Growth and Development[J].Scientia Agricultura Sinica, 2019, 52(1): 98-110.

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References 44

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指标 Index	T-AsA	AsA	DHA	AsA/DHA	T-GSH	GSH	GSSG	GSH/GSSG	MDHAR	DHAR	GR	APX	GalLDH
T-AsA	1	0.986**	0.942**	0.113	0.238	0.216	0.763*	-0.172	0.925**	0.921**	-0.395	-0.315	0.958**
AsA		1	0.957**	0.309	0.403	0.357	0.754*	-0.089	0.917**	0.929**	-0.562	-0.455	0.982**
DHA			1	0.263	0.307	0.423	0.712*	0.124	0.916**	0.925**	-0.573	-0.221	0.972**
AsA/DHA				1	-0.458	0.932**	-0.749*	0.918**	0.589	0.017	-0.918**	-0.937**	-0.562
T-GSH					1	0.961**	0.881**	0.873**	0.624*	0.009	-0.877**	-0.942**	0.934**
GSH						1	-0.291	0.925**	0.635*	0.078	-0.963**	-0.967**	0.748*
GSSG							1	-0.938**	0.872*	0.328	-0.778*	-0.872**	0.596*
GSH/GSSG								1	-0.815*	-0.152	-0.821*	-0.857**	0.834*
MDHAR									1	0.782**	-0.726**	-0.684*	0.935**
DHAR										1	-0.313	-0.176	0.942**
GR											1	0.929**	0.376
APX												1	0.818*
GalLDH													1

The Changes in the Contents of Ascorbic Acid and the Activities of Related Enzymes in Black Currant Fruits During the Process of Its Growth and Development

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[3]	CHEN XueSen, YIN HuaLin, WANG Nan, ZHANG Min, JIANG ShengHui, XU Juan, MAO ZhiQuan, ZHANG ZongYing, WANG ZhiGang, JIANG ZhaoTao, XU YueHua, LI JianMing. Interpretation of the Case of Bud Sports Selection to Promote the High-Quality and Efficient Development of the World’s Apple and Citrus Industry [J]. Scientia Agricultura Sinica, 2022, 55(4): 755-768.
[4]	XIANG MiaoLian, WU Fan, LI ShuCheng, WANG YinBao, XIAO LiuHua, PENG WenWen, CHEN JinYin, CHEN Ming. Effects of Melatonin Treatment on Resistance to Black Spot and Postharvest Storage Quality of Pear Fruit [J]. Scientia Agricultura Sinica, 2022, 55(4): 785-795.
[5]	ZHANG JinLong,ZHAO ZhiBo,LIU Wei,HUANG LiLi. The Function of Key T3SS Effectors in Pseudomonas syringae pv. actinidiae [J]. Scientia Agricultura Sinica, 2022, 55(3): 503-513.
[6]	SONG JiangTao,SHEN DanDan,GONG XuChen,SHANG XiangMing,LI ChunLong,CAI YongXi,YUE JianPing,WANG ShuaiLing,ZHANG PuFen,XIE ZongZhou,LIU JiHong. Effects of Artificial Fruit Thinning on Sugar and Acid Content and Expression of Metabolism-Related Genes in Fruit of Beni-Madonna Tangor [J]. Scientia Agricultura Sinica, 2022, 55(23): 4688-4701.
[7]	GUO ShaoLei,XU JianLan,WANG XiaoJun,SU ZiWen,ZHANG BinBin,MA RuiJuan,YU MingLiang. Genome-Wide Identification and Expression Analysis of XTH Gene Family in Peach Fruit During Storage [J]. Scientia Agricultura Sinica, 2022, 55(23): 4702-4716.
[8]	HAN DongMei,HUANG ShiLian,OUYANG SiYing,ZHANG Le,ZHUO Kan,WU ZhenXian,LI JianGuang,GUO DongLiang,WANG Jing. Optimizing Management Mode of Disease and Nutrient During the Entire Fruit Development for Improving Postharvest Storability of Longan Fruit [J]. Scientia Agricultura Sinica, 2022, 55(21): 4279-4293.
[9]	CHEN XueSen,WANG Nan,ZHANG ZongYing,MAO ZhiQuan,YIN ChengMiao. Understanding and Thinking About Some Problems of Fruit Tree Germplasm Resources and Genetic Breeding [J]. Scientia Agricultura Sinica, 2022, 55(17): 3395-3410.
[10]	HU GuangMing,ZHANG Qiong,HAN Fei,LI DaWei,LI ZuoZhou,WANG Zhi,ZHAO TingTing,TIAN Hua,LIU XiaoLi,ZHONG CaiHong. Screening and Application of Universal SSR Molecular Marker Primers in Actinidia [J]. Scientia Agricultura Sinica, 2022, 55(17): 3411-3425.
[11]	WAN LianJie,HE Man,LI JunJie,TIAN Yang,ZHANG Ji,ZHENG YongQiang,LÜ Qiang,XIE RangJin,MA YanYan,DENG Lie,YI ShiLai. Effects of Partial Substitution of Chemical Fertilizer by Organic Fertilizer on Ponkan Growth and Quality as well as Soil Properties [J]. Scientia Agricultura Sinica, 2022, 55(15): 2988-3001.
[12]	DUAN YaRu,GAO MeiLing,GUO Yu,LIANG XiaoXue,LIU XiuJie,XU HongGuo,LIU JiXiu,GAO Yue,LUAN Feishi. Map-Based Cloning and Molecular Marker Development of Watermelon Fruit Shape Gene [J]. Scientia Agricultura Sinica, 2022, 55(14): 2812-2824.
[13]	MAO LianGang,GUO MingCheng,YUAN ShanKui,ZHANG Lan,JIANG HongYun,LIU XinGang. Analysis on the Status of Insecticides Registered on Small Insects of Fruits and Vegetables in China Based on Recommended Dosage [J]. Scientia Agricultura Sinica, 2022, 55(11): 2161-2173.
[14]	LI Ang,MIAO YuLe,MENG JunRen,NIU Liang,PAN Lei,LU ZhenHua,CUI GuoChao,WANG ZhiQiang,ZENG WenFang. Peptidome Analysis of Mesocarp in Melting Flesh and Stony Hard Peach During Fruit Ripening [J]. Scientia Agricultura Sinica, 2022, 55(11): 2202-2213.
[15]	XuXian XUAN,ZiLu SHENG,ZhenQiang XIE,YuQing HUANG,PeiJie GONG,Chuan ZHANG,Ting ZHENG,Chen WANG,JingGui FANG. Function Analysis of vvi-miR172s and Their Target Genes Response to Gibberellin Regulation of Grape Berry Development [J]. Scientia Agricultura Sinica, 2021, 54(6): 1199-1217.