枣R2R3-MYB亚家族基因鉴定及其在果实发育中的表达分析

doi:10.3864/j.issn.0578-1752.2022.06.012

Abstract

Abstract:

【Objective】As one of the largest gene families in plants, MYB family plays an important role in plant growth and fruit development. This study would identify the members of the R2R3-MYB subfamily of Chinese jujube and analyze their bioinformatics and expression patterns, aiming to provide the reference for further exploring their function in jujube growth, especially in fruit development.【Method】The R2R3-MYB genes of Arabidopsis thaliana were used as probes, and this subfamily members in the jujube genome were screened out by BLAST and HMMER. Based on the Plant Transcription Factors Database, Uniprot, Gcorn Plant and other databases, R2R3-MYB genes were analyzed by MEGA-X, ExPASy, TBtools, and PheatMap. Transcriptome data and real-time quantitative PCR (qRT-PCR) were used to evaluate the expression of these genes, and the interaction proteins of key candidate proteins were predicted and verified. 【Result】In this study, 118 R2R3-MYB subfamily members were identified in jujube genome, all of which had a typical MYB domain. They were divided into 18 subgroups, named ZjMYB1-ZjMYB118. The number of amino acids encoded by the genes ranged from 200 to 400. Among them, 95 genes were located on 12 chromosomes, and which on Chr. 4 was the most widely distributed. The phylogenetic analysis showed that orthologous and paralogous events occurred during the evolution of these genes. By transcriptomic and qRT-PCR analysis, some R2R3-MYB genes were involved in the fruit development. In particular, ZjMYB59 and ZjMYB104 might participate in fruit enlargement and color development. Protein interaction prediction and yeast two-hybrid test confirmed that ZjMYB104 and ZjMPK3 were interacted each other. 【Conclusion】118 members of the R2R3-MYB subfamily members were identified and distributed on 12 chromosomes. The structure of these subfamily members was highly conservative. The orthologous and paralogous events occurred in jujube genome evolution and the candidate genes closely related to fruit development were screened out. This study provided clues and references for further functional analysis of R2R3-MYB subfamily in jujube.

Key words: Chinese jujube, R2R3-MYB subfamily, bioinformatics analysis, expression analysis, fruit development, proteins interaction

LI ShiJia,LÜ ZiJing,ZHAO Jin. Identification of R2R3-MYB Subfamily in Chinese Jujube and Their Expression Pattern During the Fruit Development[J].Scientia Agricultura Sinica, 2022, 55(6): 1199-1212.

Figures/Tables 10

Table 1

Table 2

Table 3

The information of R2R3-MYB subfamily members in jujube"

基因名称 Gene name	NCBI登录号 NCBI reference	染色体位置Chromosomes locus	阅读框长度 ORF (bp)	氨基酸数量 Amino acids size (aa)	分子量 Mw (Da)	等电点 pI	组 Group	外显子数目 Exon number
ZjMYB1	XM_016020618.2	1	1125	374	42125.68	6.41	III	3
ZjMYB2	XM_016019589.2	1	1107	368	41303.37	6.36	XI	2
ZjMYB3	XM_016026021.2	1	969	322	35915.26	6.37	III	3
ZjMYB4	XM_016031375.2	1	1098	365	41463.99	5.74	I	3
ZjMYB5	XM_016033817.2	1	1068	355	40263.36	6.46	XI	3
ZjMYB6	XM_016041113.2	1	1092	363	40879.74	7.12	I	3
ZjMYB7	XM_016044244.2	1	1551	516	56216.27	7.38	XIV	3
ZjMYB8	XM_016047554.2	1	918	305	34305.95	5.88	VIII	3
ZjMYB9	XM_016047610.2	1	918	305	34205.92	6.27	VIII	3
ZjMYB10	XM_025071868.1	1	633	210	24181.15	9.41	X	4
ZjMYB11	XM_016010160.2	1	975	324	36742.03	6.23	VIII	3
ZjMYB12	XM_016011494.2	1	600	199	22085.17	9.86	XVIII	1
ZjMYB13	XM_025070775.1	2	1545	514	56159.92	6.51	XVI	3
ZjMYB14	XM_016019055.2	2	1014	337	37964.79	6.44	III	3
ZjMYB15	XM_016020065.2	2	843	280	31740.73	8.87	II	3
ZjMYB16	XM_016020395.2	2	1182	393	43572.07	7.07	XI	2
ZjMYB17	XM_016020396.2	2	1392	463	52118.77	9.06	XI	2
ZjMYB18	XM_025072101.1	3	1068	355	40263.36	6.46	XI	5
ZjMYB19	XM_025072033.1	3	1674	557	60498.13	5.26	XIV	6
ZjMYB20	XM_016020719.2	3	1182	393	43139.24	6.24	I	3
ZjMYB21	XM_016021844.2	3	1269	422	45817.40	5.76	XVIII	2
ZjMYB22	XM_016021930.2	3	600	199	22085.17	9.86	XVIII	1
ZjMYB23	XM_025071932.1	3	540	179	19445.06	7.87	XVIII	2
ZjMYB24	XM_016022267.2	3	930	309	35021.56	5.70	XV	3
ZjMYB25	XM_016022405.2	3	483	160	18529.47	9.63	XII	2
ZjMYB26	XM_016022551.2	3	912	303	34188.03	6.80	XI	3
ZjMYB27	XM_016022613.2	3	912	303	34188.03	6.80	XI	3
ZjMYB28	XM_016022841.2	3	570	189	21194.98	4.51	XII	3
ZjMYB29	XM_016022861.2	3	816	271	30610.07	5.28	XII	2
ZjMYB30	XM_016023053.2	3	1152	383	42646.60	6.57	I	3
ZjMYB31	XM_016023353.2	4	1350	449	49829.67	6.73	VII	2
ZjMYB32	XM_016023411.2	4	918	305	35111.19	5.44	XII	3
ZjMYB33	XM_025072351.1	4	918	305	35111.19	5.44	XII	3
ZjMYB34	XM_025073253.1	4	1635	544	61347.96	8.53	XVI	3
ZjMYB35	XM_016023591.2	4	600	199	22857.66	6.66	XIII	3
ZjMYB36	XM_025072429.1	4	1038	345	38887.27	6.07	III	3
ZjMYB37	XM_025073248.1	4	1635	544	61386.09	8.53	XVI	3
ZjMYB38	XM_016023883.2	4	1053	350	38719.09	6.47	III	3
ZjMYB39	XM_016024167.2	4	996	331	37360.48	7.37	IV	3
ZjMYB40	XM_016024719.2	4	816	271	24261.55	8.65	IV	4
ZjMYB41	XM_016025498.2	4	822	273	31374.58	5.52	II	4
ZjMYB42	XM_016025511.2	4	741	246	28267.43	6.01	II	3
ZjMYB43	XM_016025514.2	4	1080	359	40269.26	5.36	I	3
ZjMYB44	XM_016025643.2	4	1647	548	59274.73	6.78	XIV	3
ZjMYB45	XM_016026187.2	5	1089	362	41025.21	5.88	XI	2
ZjMYB46	XM_016026978.2	5	1170	389	43445.01	5.40	XI	3
ZjMYB47	XM_016027029.1	5	849	282	32365.59	5.74	XVI	3
ZjMYB48	XM_016027102.2	5	801	266	30479.45	7.08	IX	3
ZjMYB49	XM_016027959.2	5	1026	341	38533.86	6.18	I	4
ZjMYB50	XM_016027963.2	5	930	309	34855.98	6.76	I	3
ZjMYB51	XM_016027995.2	5	930	309	34855.98	6.76	I	3
ZjMYB52	XM_025074262.1	5	687	228	27115.88	9.98	XVII	3
ZjMYB53	XM_016028420.2	5	747	248	29081.60	8.59	XVII	3
ZjMYB54	XM_016028861.2	6	942	313	34430.54	7.63	XVIII	1
ZjMYB55	XM_016028962.2	6	600	199	23299.35	9.36	X	3
ZjMYB56	XM_016028941.2	6	918	305	34060.09	6.71	VIII	3
ZjMYB57	XM_016029606.2	6	945	314	34389.41	6.24	III	3
ZjMYB58	XM_016029902.2	6	1041	346	38761.22	6.30	I	3
ZjMYB59	XM_016029928.2	6	693	230	25510.24	8.78	XVIII	1
ZjMYB60	XM_016030857.2	6	864	287	32390.60	8.71	IV	4
ZjMYB61	XM_016032123.2	7	891	296	33380.87	5.06	XV	3
ZjMYB62	XM_016032124.2	7	891	296	33380.87	5.06	XV	3
ZjMYB63	XM_016032235.2	7	891	296	34314.38	8.99	XIII	3
ZjMYB64	XM_016032377.2	7	822	273	30832.44	5.07	II	4
ZjMYB65	XM_016033142.2	7	999	332	36740.26	6.08	I	4
ZjMYB66	XM_016033215.2	8	882	293	33694.74	8.99	VI	2
ZjMYB67	XM_025076686.1	8	876	291	31728.46	8.32	III	3
ZjMYB68	XM_025076102.1	8	831	276	31421.30	5.45	X	2
ZjMYB69	XM_016033873.2	8	786	261	29525.12	5.81	X	3
ZjMYB70	XM_016034776.2	8	1011	336	36075.90	9.18	XVIII	1
ZjMYB71	XM_016034941.2	8	1020	339	37865.24	7.58	III	3
ZjMYB72	XM_016035211.2	8	1185	394	43392.16	6.34	XI	3
ZjMYB73	XM_016035688.2	8	831	276	31676.43	9.33	XVII	2
ZjMYB74	XM_016035781.2	9	870	289	33214.83	5.70	II	3
ZjMYB75	XM_016036214.2	9	846	281	32204.33	6.26	XIII	3
ZjMYB76	XM_016036355.2	9	1257	418	46922.01	4.78	V	3
ZjMYB77	XM_016037555.2	9	867	288	32264.01	5.84	XVIII	1
ZjMYB78	XM_016039031.1	10	771	256	29181.57	5.93	XII	3
ZjMYB79	XM_016039426.2	10	1437	478	53487.56	6.22	XVI	13
ZjMYB80	XM_016039546.2	10	1368	455	49832.18	6.19	XVII	3
ZjMYB81	XM_016039548.2	10	1215	404	44681.25	6.83	I	3
ZjMYB82	XM_016040154.2	10	936	311	35286.65	7.73	XIII	3
ZjMYB83	XM_016040524.2	11	894	297	33864.66	5.68	VI	2
ZjMYB84	XM_016041018.1	11	996	331	37414.53	6.00	XI	3
ZjMYB85	XM_016040967.2	11	1089	362	40382.56	5.40	XI	3
ZjMYB86	XM_016041507.2	11	1071	356	40987.76	9.49	XV	4
ZjMYB87	XM_025079477.1	11	1218	405	43892.18	7.07	XVIII	2
ZjMYB88	XM_016042019.2	11	1092	363	40468.92	5.42	I	3
ZjMYB89	XM_016042953.2	12	717	238	27832.61	5.11	X	4
ZjMYB90	XM_016042930.2	12	1032	343	38266.45	8.25	III	3
ZjMYB91	XM_016043881.2	12	972	323	35595.67	8.20	VII	3
ZjMYB92	XM_016044239.2	12	999	332	37141.53	6.52	III	3
ZjMYB93	XM_016044250.2	12	1188	395	43529.46	7.65	XVII	3
ZjMYB94	XM_025066858.1	12	1323	440	48646.07	8.76	V	3
ZjMYB95	XM_016044619.2	12	1044	347	38753.66	6.30	XIII	3
ZjMYB96	XM_016046006.2	Un	753	250	29105.62	7.82	IX	3
ZjMYB97	XM_016046190.2	Un	654	217	25294.42	6.95	IX	3
ZjMYB98	XM_016046427.2	Un	1434	477	53467.56	6.22	XVI	13
ZjMYB99	XM_025067712.1	Un	990	329	37401.37	4.95	XII	7
ZjMYB100	XM_016010432.2	Un	1011	336	36075.90	9.18	XVIII	1
ZjMYB101	XM_016010643.2	Un	1191	396	45085.59	5.37	II	3
ZjMYB102	XM_025068375.1	Un	750	249	28737.43	8.37	XIII	3
ZjMYB103	XM_016011368.2	Un	930	309	35021.56	5.70	XV	3
ZjMYB104	XM_016011429.2	Un	855	284	31104.15	8.98	IV	2
ZjMYB105	XM_025068805.1	Un	840	279	32350.20	5.97	IX	3
ZjMYB106	XM_016011795.2	Un	1044	347	38753.66	6.30	XIII	3
ZjMYB107	XM_016012645.2	Un	633	210	24195.17	9.41	X	4
ZjMYB108	XM_016012644.2	Un	975	324	36762.97	6.16	VIII	3
ZjMYB109	XM_016013201.2	Un	1050	349	39860.17	9.32	XVII	2
ZjMYB110	XM_016013430.2	Un	1467	488	53445.69	7.85	XI	4
ZjMYB111	XM_016013592.2	Un	669	222	26206.36	6.09	IX	3
ZjMYB112	XM_016015024.2	Un	1014	337	37307.21	6.72	III	4
ZjMYB113	XM_016015356.2	Un	2016	671	75206.30	8.84	XVI	8
ZjMYB114	XM_016016480.2	Un	747	248	29081.60	8.59	XVII	3
ZjMYB115	XM_016017001.2	Un	1467	488	53445.69	7.85	XI	3
ZjMYB116	XM_016017183.2	Un	1467	488	53445.69	7.85	XI	3
ZjMYB117	XM_016017439.1	Un	1221	406	43823.66	6.05	XI	1
ZjMYB118	XM_016017473.1	Un	1221	406	43823.66	6.05	XI	1

Table 3

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Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

References 32

[1]	GRAF T. Myb: A transcriptional activator linking proliferation and differentiation in hematopoietic cells. Centaurus; International Magazine of the History of Science and Medicine, 1992,2(2):249-255. doi: 10.1016/s0959-437x(05)80281-3. doi: 10.1016/s0959-437x(05)80281-3
[2]	DU H, ZHANG L, LIU L, TANG X F, YANG W J, WU Y M, HUANG Y B, TANG Y X. Biochemical and molecular characterization of plant MYB transcription factor family. Biochemistry Biokhimiia, 2009,74(1):1-11. doi: 10.1134/s0006297909010015. doi: 10.1134/s0006297909010015
[3]	WESTON K. Myb proteins in life, death and differentiation. Current Opinion in Genetics & Development, 1998,8(1):76-81. doi: 10.1016/s0959-437x(98)80065-8. doi: 10.1016/s0959-437x(98)80065-8
[4]	STRACKE R, WERBER M, WEISSHAAR B. The R2R3-MYB gene family in Arabidopsis thaliana. Current Opinion in Plant Biology, 2001,4(5):447-456. doi: 10.1016/s1369-5266(00)00199-0. doi: 10.1016/s1369-5266(00)00199-0
[5]	MATUS J T, AQUEA F, ARCE-JOHNSON P. Analysis of the grape MYB R2R3 subfamily reveals expanded wine quality-related clades and conserved gene structure organization across Vitis and Arabidopsis genomes. BMC Plant Biology, 2008,8:83. doi: 10.1186/1471-2229-8-83. doi: 10.1186/1471-2229-8-83
[6]	WILKINS O, NAHAL H, FOONG J, PROVART N J, CAMPBELL M M. Expansion and diversification of the Populus R2R3-MYB family of transcription factors. Plant Physiology, 2008,149(2):981-993. doi: 10.1104/pp.108.132795. doi: 10.1104/pp.108.132795
[7]	陈哲, 胡福初, 阮城城, 范鸿雁, 郭利军, 张治礼. 菠萝R2R3-MYB基因家族鉴定与表达分析. 热带作物学报, 2019,40(10):1958-1971.
	CHEN Z, HU F C, RUAN C C, FAN H Y, GUO L J, ZHANG Z L. Bioinformatics and gene expression analysis of pineapple R2R3-MYB gene family. Chinese Journal of Tropical Crops, 2019,40(10):1958-1971. (in Chinese)
[8]	刘孟军, 王玖瑞. 新中国果树科学研究70年—枣. 果树学报, 2019,36(10):1369-1381.
	LIU M J, WANG J R. Fruit scientific research in New China in the past 70 years: Chinese jujube. Journal of Fruit Science, 2019,36(10):1369-1381. (in Chinese)
[9]	DUBOS C, STRACKE R, GROTEWOLD E, WEISSHAAR B, MARTIN C, LEPINIEC L. MYB transcription factors in Arabidopsis. Trends in Plant Science, 2010,15(10):573-581. doi: 10.1016/j.tplants.2010.06.005. doi: 10.1016/j.tplants.2010.06.005
[10]	赵恩鹏, 成玉富, 杨旭. 茄科植物转录因子MYB基因家族的研究进展. 分子植物育种, 2020,19(5):1522-1530.
	ZHAO E P, CHEN Y F, YANG X. Research progress of MYB transcription factors in solanaceae plants. Molecular Plant Breeding, 2020,19(5):1522-1530. (in Chinese)
[11]	LI X L, XUE C, LI J M, QIAO X, LI L T, YU L A, HUANG Y H, WU J. Genome-wide identification, evolution and functional divergence of MYB transcription factors in Chinese white pear (Pyrus bretschneideri). Plant and Cell Physiology, 2016,57(4):824-847. doi: 10.1093/pcp/pcw029. doi: 10.1093/pcp/pcw029
[12]	AN J P, LI R, QU F J, YOU C X, WANG X F, HAO Y J. R2R3-MYB transcription factor MdMYB23 is involved in the cold tolerance and proanthocyanidin accumulation in apple. The Plant Journal, 2018,96(3):562-577. doi: 10.1111/tpj.14050. doi: 10.1111/tpj.14050
[13]	CHEN Y H, YANG X Y, HE K, LIU M H, LI J G, GAO Z F, LIN Z Q, ZHANG Y F, WANG X X, QIU X M, SHEN Y P, ZHANG L, DENG X H, LUO J C, DENG X W, CHEN Z L, GU H Y, QU L J. The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family. Plant Molecular Biology, 2006,60(1):107-124. doi: 10.1007/s11103-005-2910-y. doi: 10.1007/s11103-005-2910-y
[14]	MA D, REICHELT M, YOSHIDA K, GERSHENZON J, CONSTABEL C P. Two R2R3-MYB proteins are broad repressors of flavonoid and phenylpropanoid metabolism in poplar. The Plant Journal, 2018,96(5):949-965. doi: 10.1111/tpj.14081. doi: 10.1111/tpj.14081
[15]	朱婷婷, 梁东, 夏惠. R2R3-MYB调控果实花色苷合成的研究进展. 基因组学与应用生物学, 2016,35(4):985-991.
	ZHU T T, LIANG D, XIA H. Progress in the study of the regulation of anthocyanin synthesis by R2R3-MYB in fruits. Genomics and Applied Biology, 2016,35(4):985-991. (in Chinese)
[16]	靳亚忠, 齐娟, 何淑平, 吴兴彪, 张天一, 王雪娇, 胡文曲, 卢朝晖, 张鹏. R2R3-MYB转录因子调控果蔬品质形成的研究进展. 农业生物技术学报, 2021,29(2):364-374.
	JIN Y Z, QI J, HE S P, WU X B, ZHANG T Y, WANG X J, HU W Q, LU Z H, ZHANG P. Recent advance of R2R3-MYB transcription factors regulating the formation of fruit and vegetable qualities. Journal of Agricultural Biotechnology, 2021,29(2):364-374. (in Chinese)
[17]	李小溪. 葡萄果实莽草酸途径和类黄酮代谢协同调节机制的研究[D]. 北京: 中国农业大学, 2016.
	LI X X. Collaborative expression mechanism between shikimate pathway and flavonoid metabolism[D]. Beijing: China Agricultural University, 2016. (in Chinese)
[18]	WANG Z G, MENG D, WANG A D, LI T L, JIANG S L, CONG P H, LI T Z. The methylation of the PcMYB10 promoter is associated with green-skinned sport in max red Bartlett pear. Plant Physiology, 2013,162(2):885-896. doi: 10.1104/pp.113.214700. doi: 10.1104/pp.113.214700
[19]	周厚成, 李刚, 赵霞, 王子成, 郭蔼光. 草莓果实不同发育阶段抑制差减文库(SSH)的构建及相关基因的表达分析. 农业生物技术学报, 2013,21(6):641-649.
	ZHOU H C, LI G, ZHAO X, WANG Z C, GUO A G. Construction of suppression subtractive hybridization(SSH) cDNA library from two developmental stages of Fragaria × ananassa fruit and expression analysis of related genes. Journal of Agricultural Biotechnology, 2013,21(6):641-649. (in Chinese)
[20]	WANG H, ZHANG H, YANG Y, LI M F, ZHANG Y T, LIU J S, DONG J, LI J, BUTELLI E, XUE Z, WANG A M, WANG G X, MARTIN C, JIN W M. The control of red colour by a family of MYB transcription factors in octoploid strawberry (Fragaria × ananassa) fruits. Plant Biotechnology Journal, 2020,18(5):1169-1184. doi: 10.1111/pbi.v18.5
[21]	ESPLEY R V, BRENDOLISE C, CHAGNÉ D, KUTTY-AMMA S, GREEN S, VOLZ R, PUTTERILL J, SCHOUTEN H J, GARDINER S E, HELLENS R P, ALLAN A C. Multiple repeats of a promoter segment causes transcription factor autoregulation in red apples. The Plant Cell, 2009,21(1):168-183. doi: 10.1105/tpc.108.059329. doi: 10.1105/tpc.108.059329
[22]	JI Q, WANG D W, ZHOU J, XU Y L, SHEN B Q, ZHOU F. Genome-wide characterization and expression analyses of the MYB superfamily genes during developmental stages in Chinese jujube. PeerJ, 2019,7(1):6353.
[23]	CHEN C J, CHEN H, ZHANG Y, THOMAS H R, FRANK M H, HE Y H, XIA R. TBtools: an integrative toolkit developed for interactive analyses of big biological data. Molecular Plant, 2020,13(8):1194-1202. doi: 10.1016/j.molp.2020.06.009. doi: 10.1016/j.molp.2020.06.009
[24]	赵亚梅, 陈蕾, 吴春梅, 秦思, 翟俊文, 任惠, 吴沙沙. 阳桃R2R3-MYB家族成员鉴定及其在木质素合成过程中的表达. 植物遗传资源学报, 2021(6):1-19.
	ZHAO Y M, CHEN L, WU C M, QIN S, ZHAI J W, REN H, WU S S. Identification of the R2R3-MYB family members in Averrhoa carambola and their expression involved in lignin biosynthesis. Journal of Plant Genetic Resources, 2021(6):1-19. (in Chinese)
[25]	周婵, 陈超, 左静, 晏晓兰, 张俊卫, 包满珠. 梅R2R3型MYB转录因子的鉴定及低温胁迫下的表达分析. 北京林业大学学报, 2015,37(S1):61-65.
	ZHOU C, CHEN C, ZUO J, YAN X L, ZHANG J W, BAO M Z. Identification and expression analysis of R2R3-MYB transcription factors in Prunus mume under cold treatment. Journal of Beijing Forestry University, 2015,37(S1):61-65. (in Chinese)
[26]	石倩倩. 枣果实色泽性状形成的分子机制研究[D]. 杨凌: 西北农林科技大学, 2019.
	SHI Q Q. Molecuar mechanism of the formation of fruit pigment in jujube fruits[D]. Yangling: North West Agriculture and Forestry University, 2019. (in Chinese)
[27]	张琼. 枣着色过程中果皮结构及色素积累相关组分研究[D]. 保定: 河北农业大学, 2020.
	ZHANG Q. Analysis of structure and components related to pigment accumlation during jujube coloring[D]. Baoding: Hebei Agricultural University, 2020. (in Chinese)
[28]	陈昕, 陈蕊红, 黄建. 枣果实不同发育阶段蛋白质组动态研究. 西北林学院学报, 2019,34(4):82-90. doi: 10.3969/j.issn.1001-7461.2019.04.12. doi: 10.3969/j.issn.1001-7461.2019.04.12
	CHEN X, CHEN R H, HUANG J. Comparative proteomic analysis on Chinese jujube fruit at different developmental and ripening stages. Journal of Northwest Forestry University, 2019,34(4):82-90. doi: 10.3969/j.issn.1001-7461.2019.04.12. (in Chinese) doi: 10.3969/j.issn.1001-7461.2019.04.12
[29]	ZHAI R, ZHAO Y X, WU M, YANG J, LI X Y, LIU H T, WU T, LIANG F F, YANG C Q, WANG Z G, MA F W, XU L F. The MYB transcription factor PbMYB12b positively regulates flavonol biosynthesis in pear fruit. BMC Plant Biology, 2019,19(1):85. doi: 10.1186/s12870-019-1687-0. doi: 10.1186/s12870-019-1687-0
[30]	RAHIM M A, RESENTINI F, DALLA VECCHIA F, TRAINOTTI L. Effects on plant growth and reproduction of a peach R2R3-MYB transcription factor overexpressed in tobacco. Frontiers in Plant Science, 2019,10:1143. doi: 10.3389/fpls.2019.01143. doi: 10.3389/fpls.2019.01143
[31]	韩瑜. FaSnRK2.6/FaMPK3信号系统在草莓果实发育和成熟中的作用及机理分析[D]. 北京: 中国农业大学, 2015.
	HAN Y. The function and mechanism analysis of FaSnRK2.6/ FaMPK3 signaling system during fruit development and ripening in Fragaria×ananassa [D]. Beijing: China Agricultural University, 2015. (in Chinese)
[32]	叶婷. 番茄SlMAPK11通过与SlSnRK1互作调控果实成熟机理研究[D]. 武汉: 华中农业大学, 2020.
	YE T. Mechanisms of SlMAPK11 regulate fruit ripening via interacting with SlSnRK1 in tomato[D]. Wuhan: Huazhong Agricultural University, 2020. (in Chinese)

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引物名称 Primer name	引物序列 Primer sequence (5′-3′)
ACT-F	AGCCTTCCTGCCAACGAGT
ACT-R	TTGCTTCTCACCCTTGATGC
ZjMYB59-F	GGGTTTTGACCCGTCTTGTTG
ZjMYB59-R	CCTGTTACCATACCTGGCGT
ZjMYB104-F	CACCACCACCTCAGGAAACA
ZjMYB104-R	ACGACGAGGTCCTTTCCAAC

引物名称 Primer name	引物序列 Primer sequence (5′-3′)
ZjMYB104-F	ATGGGAAGGTCTCCATGTTGT
ZjMYB104-R	TTATTTCATTTCCAAGCCTCTATAATCC
AD1-F	GTACCAGATTACGCTCATATGGGAAGGTCTCCATGTTGT
AD1-R	ACTGGCCTCCATGGCCATTTATTTCATTTCCAAGCCTCTATAATCC
BD1-F	TCAGAGGAGGACCTGCATATGGGAAGGTCTCCATGTTGT
BD1-R	TTCGGCCTCCATGGCCATTTATTTCATTTCCAAGCCTCTATAATCC
ZjMPK3-F	ATGTCGGGTGCTAACCAGAC
ZjMPK3-R	TCAGGCATACTCCGGATTAAGTG
AD2-F	GCCATGGAGGCCAGTGAAATGTCGGGTGCTAACCAGAC
AD2-R	ATGCCCACCCGGGTGGAATCAGGCATACTCCGGATTAAGTG
BD2-F	ATGGCCATGGAGGCCGAAATGTCGGGTGCTAACCAGAC
BD2-R	ACGGATCCCCGGGAATTCTCAGGCATACTCCGGATTAAGTG

Identification of R2R3-MYB Subfamily in Chinese Jujube and Their Expression Pattern During the Fruit Development

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