人工疏果对‘爱媛28’橘橙果实糖酸含量及代谢基因表达的影响

doi:10.3864/j.issn.0578-1752.2022.23.010

Abstract

Abstract: 【Background】 Beni-Madonna tangor (Citrus nanko × C. amakusa), a hybrid cultivar of citrus, is favored by consumers because of its excellent quality. Fruit thinning is a technique commonly used to improve fruit quality in the process of agricultural production. It can not only significantly increase the fruit volume, but also increase the content of soluble solids in the fruit. However, the specific mechanism of fruit thinning to increase the content of soluble solids in the fruit of Beni-Madonna is still unclear. 【Objective】 In this study, four-year-old Beni-Madonna tangor plants, with trifoliate orange (Poncirus trifoliata) as a base rootstock and Ponkan as an intermediate stock, were used as experimental materials to explore the specific mechanism of fruit thinning on affecting the change of sugar and acid content in fruit. 【Method】Fruit thinning was carried out at the young fruit stage of Beni-Madonna fruit. The horizontal and vertical stems of fruit thinning and non-fruit thinning were measured every half a month or so. The samples were taken back and the single fruit weight and sugar and acid content were measured. The activities of sugar and acid metabolism related enzymes and the relative expression of their corresponding coding genes were measured when the sugar and acid content were significantly different..【Result】 The fruit thinning significantly increased the horizontal and vertical stems and single fruit weight of Beni-Madonna fruit in the middle and late stages of fruit growth, significantly accelerated the degradation rate of citrate content, but did not affect the final citrate content at fruit maturity, significantly increased the fruit glucose and sucrose content, but had no significant effect on fructose content. The fruit thinning significantly increased the activities of sucrose synthase SSⅡ (synthesis direction), sucrose synthase SSⅠ (decomposition direction), sucrose phosphate synthase (SPS) in sucrose metabolism and cytosolic aconitase (Cyt-ACO), cytoplasmic isocitrate dehydrogenase (ICDH) and glutamine synthetase (GS) implicated in citric acid catabolism. In addition, fruit thinning significantly promoted the early relative expression of CitSS3 and the relative expression of CitSPSs at all stages in the process of sucrose metabolism, and the relative expression of CitACO3, CitNADP-IDH1, CitACLα1/β, CitGAD1 and CitGSs in the process of citric acid metabolism in the early and middle stages..【Conclusion】 The artificial fruit thinning mainly promoted the accumulation of soluble sugar in fruit by enhancing the enzyme activity of sucrose phosphate synthase in the process of sucrose anabolism, improved the activity and gene expression level of related enzymes in the process of citric acid catabolism, and accelerated citric acid degradation, thus playing an important role in improving fruit quality.

Key words: fruit thinning, Beni-Madonna, fruit quality, sucrose metabolism, citric acid metabolism

SONG JiangTao,SHEN DanDan,GONG XuChen,SHANG XiangMing,LI ChunLong,CAI YongXi,YUE JianPing,WANG ShuaiLing,ZHANG PuFen,XIE ZongZhou,LIU JiHong. Effects of Artificial Fruit Thinning on Sugar and Acid Content and Expression of Metabolism-Related Genes in Fruit of Beni-Madonna Tangor[J].Scientia Agricultura Sinica, 2022, 55(23): 4688-4701.

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References 30

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基因 Gene	上游引物Forward primer (5′ to 3′)	下游引物Reverse primer (3′ to 5′)
CitSS1	ACCAGTAGTTCATGCCACCG	GCGAAAAGGACCACCCTGTA
CitSS2	GTTGAAAGGCACTGACACGC	CCAATGGAGGGGGTTTTGCT
CitSS3	CTTCAACGGGTTCCTCTGCT	TTCCCACAGGTGTTTTCGGT
CitSS4	AGCGTGTGGTAATGCTGGAA	TGCCTTAGCCAGCAATCCTT
CitSPS1	TCTCCACCGTTCCTGGGTTA	ACGACGTTTCGCCATTCTCT
CitSPS2	TTTCATGGCCTGAGCACTGT	CGTTTTTGTCGCCTTCCAGG
CitSPS3	CGCATTTGGCATCTTACCCG	CCTTGTTCCCGCTCCAATCT
CitSPS4	ATATGTGCTGGCGCATTTGG	CGGCATCATTACGACCTTGC
CitVINV	GGTGTTACATGGCGTCCCTA	TGGCACCCATGTCACATTCT
CitCINV	TGGGAGAAGACTGTGGACTG	GCAACACGACCAATGGCTGA
CitPEPC1	GTGCGATCCCGTCTATCTGT	AAGGCTCAAGGCCACTTTTT
CitPEPC2	GGCATGCAAAACACTGGTTA	CATGTTCATTACGGCTTGGA
CitPEPC3	GAACAATGACGGACACAACG	TGGACTCGCTTCCAACTTCT
CitCS1	GGTGCCCCCAATATTAACAA	AGAGCTCGGTCCCATATCAA
CitCS2	ACTGGTGTATGGATGCGACA	TCTTCGTCTTGTGGCATTTG
CitNADPIDH1	GAAAATTGGGGATTGGGATT	CAACAGAGGTGCAGCTCAAA
CitNADPIDH2	CAGCGGACATGTGAACAATC	CCGTCCATTTCAACGATAGG
CitNADPIDH3	TACCGGGTTCATCAGAAAGG	AGGCTGCTTCCAGTTTCTCA
CitACO1	GGCAAGTCATTCACATGCGTT	TGAAGAAGTAGACCCCGGTTGA
CitACO2	GGCAATGATGAAGTGATGGCT	GTTGGAACATGGACCGTCTTT
CitACO3	TGCAGCAATGAGGTACAAGGC	TCACACCCAGAAGCATTGGAC
CitACLα2	TACAGTGGAGCACCCAACGA	CCTTCAGGGCTTGGATTATG
CitACLα1	GATACTGTTGGAGACTTGGG	GCTCTCTTACGACCATCAGG
CitACLβ	GAGGAGATAACAGAGACAAA	AACAAAGAGCCCATTCAGAT
CitGAD1	CACCAAAAAGAATGAGGAGACC	CCGTACTTGTGACCACTGACAT
CitGAD2	ACCGCAATGTGATGGAGAA	GAATTCATCGTGGCGTTTG
CitGS1	CATCAATGCTATCGCGTGTT	TCTGCATTCTTGGCAGGTTA
CitGS2	TTTGGGATGCTCAGTTGTGA	CTGAATGGCTCCCAAAAATG

酶 Enzyme	相关系数 Pearson correlation
酶 Enzyme	对照 CK	疏果 Fruit thinning
S-AI	0.213	0.424
SPS	0.829	0.996**
SS I	0.629	-0.032
SS II	0.887	0.961*
CS	0.405	0.539
ACL	0.995**	0.693
ACO	-0.891	-0.977*
GAD	0.863	0.827
GS	0.058	-0.556
PEPC	0.928	0.894
NADP-IDH	-0.860	-0.787

基因 Gene	相关系数 Pearson correlation
基因 Gene	对照 CK	疏果 Fruit thinning
CitSS1	-0.019	0.360
CitSS2	0.329	0.567
CitSS3	0.713	0.943
CitSS4	-0.026	0.972*
CitSPS1	0.596	0.844
CitSPS2	0.541	0.764
CitSPS3	0.602	0.842
CitSPS4	0.449	0.726
CitVINV	0.532	0.692
CitCINV	0.840	0.717
CitPEPC1	0.666	0.746
CitPEPC2	-0.738	-0.626
CitPEPC3	-0.289	0.018
CitCS1	-0.164	-0.652
CitCS2	-0.620	-0.957*
CitNADPIDH1	-0.921	-0.897
CitNADPIDH2	-0.481	-0.672
CitNADPIDH3	-0.870	-0.666
CitACO1	-0.710	-0.690
CitACO2	-0.311	-0.752
CitACO3	-0.761	-0.752
CitACLα2	0.589	-0.145
CitACLα1	-0.502	-0.374
CitACLβ	-0.747	-0.992**
CitGAD1	-0.774	0.465
CitGAD2	-0.788	-0.685
CitGS1	-0.856	-0.593
CitGS2	-0.999**	-0.132

Effects of Artificial Fruit Thinning on Sugar and Acid Content and Expression of Metabolism-Related Genes in Fruit of Beni-Madonna Tangor

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