Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (20): 3871-3884.doi: 10.3864/j.issn.0578-1752.2017.20.004


Effects of Exogenous 6-BA on Photosynthetic Characteristics and Endogenous Hormone Content in Wheat Leaves Under Two Nitrogen Application Levels at Seedling Stage

YANG DongQing1, DONG WenHua1,LUO YongLi1, 2, SONG WenTing1, CAI Te3, LI Yong1, YIN YanPing1, WANG ZhenLin1   

  1. 1College of Agronomy, Shandong Agricultural University/State Key Laboratory of Crop Biology, Tai’an 271018, Shandong;  2College of Life Sciences, Shandong Agricultural University, Tai’an 271018, Shandong; 3 College of Agronomy, Northwest      A&F University, Yangling 712100, Shaanxi
  • Received:2017-02-27 Online:2017-10-16 Published:2017-10-16

Abstract: 【Objective】The purpose of this study was to test the effects of nitrogen concentrations and spraying exogenous cytokinin (6-BA) on photosynthetic characteristics, chlorophyll a fluorescence, nitrate reductase (NR) and glutamine synthetase (GS) activities, changes of endogenous hormones in leaves of wheat at seedling stage and grain yield, and then provide a theoretical basis for improving the development of winter wheat seedlings. 【Method】Wheat (Jimai 22) was sown in the field and pot culture experiments with two nitrogen levels ( N1, 120 kg·hm-2; N2, 240 kg·hm-2) and modified Hoagland nutrient solutions containing two nitrogen levels (HN, 3.75 mmol·L-1; LN, 0.63 mmol·L-1), respectively. 6-BA (30 mg·L-1) and lovastatin (300 mg·L-1) were sprayed to the whole plants at a rate of 100 mL·m-2 at the three-leaf stage. Ten wheat plants were sampled at 3 d intervals to test chlorophyll contents, NR and GS activities, photosynthetic parameters (Gs, Tr, Ci and Pn), chlorophyll fluorescence parameters (Wk, Vj, Ψo and PIabs), and endogenous hormones. In addition, grain yield, kernels per spike, 1000-grain weight, and ear numbers were determined at maturity stage.【Result】The above ground biomass (AGBM) was significantly increased by exogenous 6-BA treatment in the pot experiment. Conversely, application of lovastatin significantly decreased AGBM. Compared with HN treatment, AGBM under HN+6-BA treatment increased by 21.39% at 12 days after treatment (DAT), and that of LN+6-BA treatment increased by 43.92%, compared with LN treatment. Application of 6-BA significantly increased values of Gs, Tr, Ci and Pn under high nitrogen condition. Especially, Gs, Tr, Pn and Ci increased by 68.32%、58.66%、30.72%、51.61% at 12 DAT, respectively. Chl a increased by application of 6-BA at 9 to 12 DAT, while content of Chl b has significantly increased by exogenous 6-BA from 3 to 12 DAT. Conversely, both Chl a and Chl b have significantly decreased by application of lovastatin. Compared with HN treatment, HN+6-BA treatment significantly increased the activity of nitrate reductase (NR) and glutamine synthetase (GS). While application of lovastatin significantly decreased NR and GS activity. Exogenous 6-BA treatments changed the fast chlorophyll fluorescence induction kinetics curves. Application of 6-BA significantly increased Ψo and PIabs, and decreased Wk and Vj. Wk and Vj decreased by 22.09% and 36.05%, respectively, under LN+6-BA treatment, compared with LN treatment. Application of 6-BA significantly increased Zt content from 3 to 12 DAT, increased IAA content from 6 to 12 DAT, and decreased ABA content. However, spraying exogenous lovastatin obviously increased ABA content. Application of 6-BA significantly increased grain yield in the field experiment. Compared with N1 and N2, grain yield of N1+6-BA and N2+6-BA increased by 10.48% and 16.61%, respectively. 【Conclusion】Exogenous 6-BA increased aboveground biomass due to increasing the leaf photosynthesis and nitrogen assimilation through regulating endogenous hormones contents to enhance chlorophyll content and NR and GS activity, and to improve the electron transfer capability of both the donor and the acceptor sides at PSII reaction center resulting in improved PSII performance. Grain yield has significantly increased under 6-BA combined with nitrogen application treatments.

Key words: wheat seedling, nitrogen, hormone, photosynthetic characteristics, chlorophyll a fluorescence

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