甘蓝型油菜苗期氮高效吸收转运特征研究

doi:10.3864/j.issn.0578-1752.2022.06.010

Abstract

Abstract:

【Objective】Nitrogen (N) uptake efficiency is one of the important factors affecting crop N efficiency, investigating characteristics of efficient N uptake and transport was the purpose to provide the theoretical basis for improving N efficiency and yield of rapeseed (Brassica napus L.) varieties.【Method】To explore the mechanisms underlying high N uptake and transport in rapeseed, two rapeseed germplasms with contrasting N efficiency (N efficient germplasm ‘498’ and N inefficient germplasm ‘428’) were used in this study under normal N (9.5 mmol·L^-1) and low N (0.475 mmol·L^-1) conditions at three different growth stages (Phenological growth stages 12, 14 and 16) in hydroponic culture. At the same time, the ¹⁵N isotope tracer technique was applied to study the uptake and transport capacity of NO₃^- and NH₄⁺. Additionally, the expression level of genes (BnNPFs, BnNRT2s and BnAMTs）related to N uptake and transport in rapeseed germplasms with contrasting N efficiency were further analyzed by real-time quantitative PCR (RT-qPCR).【Result】Rapeseed germplasm ‘498’ showed superior advantages in plant growth and root development under different N concentrations, and the root morphological indexes (main root length, total root length, root surface area, root volume and lateral root number), biomass, N accumulation and N uptake efficiency were all significantly greater than those of germplasm ‘428’.¹⁵N isotope tracer test also showed that ‘498’ showed greater advantage in the uptake and accumulation of NO₃ ^- and NH₄⁺, especially for NH₄⁺, as indicated by the significant differences in the accumulation of ¹⁵NH₄⁺ between two germplasms. The RT-qPCR analysis further found that under normal N conditions, the relative expressions of BnNPF6.3a, BnNRT2.1e, BnNPF7.2a, BnNPF7.2c, BnNPF6.2c, BnAMT1;2a, BnAMT1;3c, BnAMT1;4a, BnAMT2;1a and BnAMT2;1b (involved in the uptake and transport of NO₃ ^- and NH₄⁺) was significantly higher in ‘498’ than that in ‘428’. While under low N stress, the relative expressions of BnNRT2.4a, BnNRT2.5a and BnNRT2.5b (involved in NO₃ ^-uptake and transport) was significantly lower in the root of ‘498’ than that of ‘428’, but the expression level of BnNPF7.3a and BnNPF6.2c (referred to NO₃ ^- transport and redistribution) was significantly higher in ‘498’ than that in ‘428’, as well as the expression level of BnAMT1;1a, BnAMT1;2a, BnAMT1;3c, BnAMT1;4a, BnAMT2;1a and BnAMT2;1b (involved in NH₄ ⁺ uptake and transport).【Conclusion】Compared with N-inefficient germplasm ‘428’, N-efficient germplasm ‘498’ were superior in root length, root surface area (volume) and lateral root number, additionally with greater ability in N (especially NH₄ ⁺) uptake and accumulation. Under normal N application conditions, the expression of genes involved in NO₃^- and NH₄⁺ absorption and transport were relatively higher in ‘498’, while the relative expression of genes involved in the NO₃ ^- transport and redistribution as well as NH₄⁺ absorption and transport were significantly higher in ‘498’ than that in ‘428’ under low N stress, illustrating the relative higher N uptake efficiency of ‘498’ possibly linked to the higher expressions of several BnNPFs sand BnAMTs.

Key words: rapeseed, high nitrogen efficiency, root morphology, uptake and transport, nitrogen transporter genes

CHAO ChengSheng,WANG YuQian,SHEN XinJie,DAI Jing,GU ChiMing,LI YinShui,XIE LiHua,HU XiaoJia,QIN Lu,LIAO Xing. Characteristics of Efficient Nitrogen Uptake and Transport of Rapeseed at Seedling Stage[J].Scientia Agricultura Sinica, 2022, 55(6): 1172-1188.

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References 51

[1]	王汉中. 以新需求为导向的油菜产业发展战略. 中国油料作物学报, 2018,40(5):613-617. doi: 10.7505/j.issn.1007-9084.2018.05.001. doi: 10.7505/j.issn.1007-9084.2018.05.001
	WANG H Z. New-demand oriented oilseed rape industry developing strategy. Chinese Journal of Oil Crop Sciences, 2018,40(5):613-617. doi: 10.7505/j.issn.1007-9084.2018.05.001. (in Chinese) doi: 10.7505/j.issn.1007-9084.2018.05.001
[2]	BOUCHET A S, LAPERCHE A, BISSUEL-BELAYGUE C, SNOWDON R, NESI N, STAHL A. Nitrogen use efficiency in rapeseed. A review. Agronomy for Sustainable Development, 2016,36(2):1-20. doi: 10.1007/s13593-016-0371-0. doi: 10.1007/s13593-016-0371-0
[3]	LASSALETTA L, BILLEN G, GRIZZETTI B, ANGLADE J, GARNIER J. 50 year trends in nitrogen use efficiency of world cropping systems: the relationship between yield and nitrogen input to cropland. Environmental Research Letters, 2014,9(10):105011. doi: 10.1088/1748-9326/9/10/105011. doi: 10.1088/1748-9326/9/10/105011
[4]	朱兆良, 金继运. 保障我国粮食安全的肥料问题. 植物营养与肥料学报, 2013,19(2):259-273. doi: 10.11674/zwyf.2013.0201. doi: 10.11674/zwyf.2013.0201
	ZHU Z L, JIN J Y. Fertilizer use and food security in China. Plant Nutrition and Fertilizer Science, 2013,19(2):259-273. doi: 10.11674/zwyf.2013.0201. (in Chinese) doi: 10.11674/zwyf.2013.0201
[5]	XU G H, FAN X R, MILLER A J. Plant nitrogen assimilation and use efficiency. Annual Review of Plant Biology, 2012,63:153-182. doi: 10.1146/annurev-arplant-042811-105532. doi: 10.1146/annurev-arplant-042811-105532
[6]	康亮, 梁琼月, 姚一华, 蒋强, 董蒙蒙, 顾明华, 何冰. 不同氮效率木薯品种根系形态、构型及氮吸收动力学特征. 植物营养与肥料学报, 2019,25(11):1920-1928. doi: 10.11674/zwyf.19024. doi: 10.11674/zwyf.19024
	KANG L, LIANG Q Y, YAO Y H, JIANG Q, DONG M M, GU M H, HE B. Root morphology, configuration and nitrogen absorption kinetics of cassava cultivars with different nitrogen efficiencies. Journal of Plant Nutrition and Fertilizers, 2019,25(11):1920-1928. doi: 10.11674/zwyf.19024. (in Chinese) doi: 10.11674/zwyf.19024
[7]	刘代平, 宋海星, 刘强, 荣湘民, 彭建伟, 谢桂先, 刘浩荣. 油菜根系形态和生理特性与其氮效率的关系. 土壤, 2008,40(5):765-769. doi: 10.3321/j.issn:0253-9829.2008.05.015. doi: 10.3321/j.issn:0253-9829.2008.05.015
	LIU D P, SONG H X, LIU Q, RONG X M, PENG J W, XIE G X, LIU H R. Relationship between root morphologic and physiological properties and nitrogen efficiency of oilseed rape cultivars. Soils, 2008,40(5):765-769. doi: 10.3321/j.issn:0253-9829.2008.05.015. (in Chinese) doi: 10.3321/j.issn:0253-9829.2008.05.015
[8]	熊淑萍, 吴克远, 王小纯, 张捷, 杜盼, 吴懿鑫, 马新明. 不同氮效率基因型小麦根系吸收特性与氮素利用差异的分析. 中国农业科学, 2016,49(12):2267-2279. doi: 10.3864/j.issn.0578-1752.2016.12.003. doi: 10.3864/j.issn.0578-1752.2016.12.003
	XIONG S P, WU K Y, WANG X C, ZHANG J, DU P, WU Y X, MA X M. Analysis of root absorption characteristics and nitrogen utilization of wheat genotypes with different N efficiency. Scientia Agricultura Sinica, 2016,49(12):2267-2279. doi: 10.3864/j.issn.0578-1752.2016.12.003. (in Chinese) doi: 10.3864/j.issn.0578-1752.2016.12.003
[9]	WANG G L, DING G D, LI L, CAI H M, YE X S, ZOU J, XU F S. Identification and characterization of improved nitrogen efficiency in interspecific hybridized new-type Brassica napus. Annals of Botany, 2014,114(3):549-559. doi: 10.1093/aob/mcu135. doi: 10.1093/aob/mcu135
[10]	KAMH M, WIESLER F, ULAS A, HORST W J. Root growth and N-uptake activity of oilseed rape (Brassica napus L.) cultivars differing in nitrogen efficiency. Journal of Plant Nutrition and Soil Science, 2005,168(1):130-137. doi: 10.1002/jpln.200421453. doi: 10.1002/jpln.200421453
[11]	刘强, 宋海星, 荣湘民, 彭建伟, 谢桂先. 不同品种油菜氮效率差异及其生理基础研究. 植物营养与肥料学报, 2008,14(1):113-119. doi: 10.3321/j.issn:1008-505X.2008.01.018. doi: 10.3321/j.issn:1008-505X.2008.01.018
	LIU Q, SONG H X, RONG X M, PENG J W, XIE G X. Differences in nitrogen use efficiency among different rape varieties and their physiological basis. Plant Nutrition and Fertilizer Science, 2008,14(1):113-119. doi: 10.3321/j.issn:1008-505X.2008.01.018. (in Chinese) doi: 10.3321/j.issn:1008-505X.2008.01.018
[12]	洪娟. 油菜氮高效种质的筛选及其生理机制的初步研究[D]. 武汉: 华中农业大学, 2007.
	HONG J. Screening of nitrogen efficient germplasm and preliminary study on its phsiological mechanism in rapeseed[D]. Wuhan: Huazhong Agricultural University, 2007. (in Chinese)
[13]	TENG W, HE X, TONG Y P. Transgenic approaches for improving use efficiency of nitrogen, phosphorus and potassium in crops. Journal of Integrative Agriculture, 2017,16(12):2657-2673. doi: 10.1016/S2095-3119(17)61709-X. doi: 10.1016/S2095-3119(17)61709-X
[14]	FAN X R, NAZ M, FAN X R, XUAN W, MILLER A J, XU G H. Plant nitrate transporters: from gene function to application. Journal of Experimental Botany, 2017,68(10):2463-2475. doi: 10.1093/jxb/erx011. doi: 10.1093/jxb/erx011
[15]	TSAY Y F, CHIU C C, TSAI C B, HO C H, HSU P K. Nitrate transporters and peptide transporters. FEBS Letters, 2007,581(12):2290-2300. doi: 10.1016/j.febslet.2007.04.047. doi: 10.1016/j.febslet.2007.04.047
[16]	LIU K H, TSAY Y F. Switching between the two action modes of the dual-affinity nitrate transporter CHL1 by phosphorylation. The EMBO Journal, 2003,22(5):1005-1013. doi: 10.1093/emboj/cdg118. doi: 10.1093/emboj/cdg118
[17]	LÉRAN S, VARALA K, BOYER J C, CHIURAZZI M, CRAWFORD N, DANIEL-VEDELE F, DAVID L, DICKSTEIN R, FERNANDEZ E, FORDE B, GASSMANN W, GEIGER D, GOJON A, GONG J M, HALKIER B A, HARRIS J M, HEDRICH R, LIMAMI A M, LACOMBE B. A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family members in plants. Trends in Plant Science, 2014,19(1):5-9. doi: 10.1016/j.tplants.2013.08.008. doi: 10.1016/j.tplants.2013.08.008
[18]	LUDEWIG U, NEUHÄUSER B, DYNOWSKI M. Molecular mechanisms of ammonium transport and accumulation in plants. FEBS Letters, 2007,581(12):2301-2308. doi: 10.1016/j.febslet.2007.03.034. doi: 10.1016/j.febslet.2007.03.034
[19]	HAN Y L, SONG H X, LIAO Q, YU Y, JIAN S F, LEPO J E, LIU Q, RONG X M, TIAN C, ZENG J, GUAN C Y, ISMAIL A M, ZHANG Z H. Nitrogen use efficiency is mediated by vacuolar nitrate sequestration capacity in roots of Brassica napus. Plant Physiology, 2016,170(3):1684-1698. doi: 10.1104/pp.15.01377. doi: 10.1104/pp.15.01377
[20]	张玉莹. 不同氮效率油菜NRT基因的表达差异研究[D]. 杨凌: 西北农林科技大学, 2013.
	ZHANG Y Y. The expression differences study on the NRT rape genes of different nitrogen efficiency[D]. Yangling: Northwest A & F University, 2013. (in Chinese)
[21]	徐子先. 甘蓝型油菜氮效率评价及其差异的生理机制探究[D]. 北京: 中国农业科学院, 2017.
	XU Z X. Researches on nitrogen efficiency evaluation in rapeseed (Brassica napus L.) germplasm and its physiological mechanism[D]. Beijing: Chinese Academy of Agricultural Sciences, 2017. (in Chinese)
[22]	MEIER U. Growth stages of mono-and dicotyledonous plants. 2001. https://www.politicheagricole.it/flex/AppData/WebLive/Agrometeo/MIEPFY800/BBCHengl2001.pdf.
[23]	鲍士旦. 土壤农化分析. 3版. 北京: 中国农业出版社, 2000.
	BAO S D. Soil and Agricultural Chemistry Analysis. Beijing: Chinese Agriculture Press, 2000. (in Chinese)
[24]	顾炽明, 韩配配, 胡琼, 李银水, 廖祥生, 张志华, 谢立华, 胡小加, 秦璐, 廖星. 甘蓝型油菜苗期氮效率评价. 中国油料作物学报, 2018,40(6):851-860. doi: 10.7505/j.issn.1007-9084.2018.06.015. doi: 10.7505/j.issn.1007-9084.2018.06.015
	GU C M, HAN P P, HU Q, LI Y S, LIAO X S, ZHANG Z H, XIE L H, HU X J, QIN L, LIAO X. Nitrogen efficiency evaluation in rapeseed (Brassica napus L.) at seedling stage. Chinese Journal of Oil Crop Sciences, 2018,40(6):851-860. doi: 10.7505/j.issn.1007-9084.2018.06.015. (in Chinese) doi: 10.7505/j.issn.1007-9084.2018.06.015
[25]	王改丽. 新型甘蓝型油菜氮高效种质的筛选及其氮高效机制的研究[D]. 武汉: 华中农业大学, 2014.
	WANG G L. Screening of high nitrogen efficient germplasms and its mechanism in new-type Brassica napus[D]. Wuhan: Huazhong Agricultural University, 2014. (in Chinese)
[26]	晏娟, 沈其荣, 尹斌, 万新军. 应用15N示踪技术研究水稻对氮肥的吸收和分配. 核农学报, 2009,23(3):487-491, 496.
	YAN J, SHEN Q R, YIN B, WAN X J. Fertilizer-n uptake and distribution in rice plants using 15n tracer technique. Journal of Nuclear Agricultural Sciences, 2009,23(3):487-491, 496. (in Chinese)
[27]	O'BRIEN J A, VEGA A, BOUGUYON E, KROUK G, GOJON A, CORUZZI G, GUTIÉRREZ R A. Nitrate transport, sensing, and responses in plants. Molecular Plant, 2016,9(6):837-856. doi: 10.1016/j.molp.2016.05.004. doi: 10.1016/j.molp.2016.05.004
[28]	LU K, LI T, HE J, CHANG W, ZHANG R, LIU M, YU M N, FAN Y H, MA J Q, SUN W, QU C M, LIU L Z, LI N N, LIANG Y, WANG R, QIAN W, TANG Z L, XU X F, LEI B, ZHANG K, LI J N. qPrimerDB: a thermodynamics-based gene-specific qPCR primer database for 147 organisms. Nucleic Acids Research, 2017,46(D1):D1229-D1236. doi: 10.1093/nar/gkx725. doi: 10.1093/nar/gkx725
[29]	TONG J F, WALK T C, HAN P P, CHEN L Y, SHEN X J, LI Y S, GU C M, XIE L H, HU X J, LIAO X, QIN L. Genome-wide identification and analysis of high-affinity nitrate transporter 2 (NRT2) family genes in rapeseed (Brassica napus L.) and their responses to various stresses. BMC Plant Biology, 2020,20(1):464. doi: 10.1186/s12870-020-02648-1. doi: 10.1186/s12870-020-02648-1
[30]	HU J H, ZHANG F G, GAO G Z, LI H, WU X M. Auxin-related genes associated with leaf petiole angle at the seedling stage are involved in adaptation to low temperature in Brassica napus. Environmental and Experimental Botany, 2021,182:104308. doi: 10.1016/j.envexpbot.2020.104308. doi: 10.1016/j.envexpbot.2020.104308
[31]	LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method. Methods, 2001,25(4):402-408. doi: 10.1006/meth.2001.1262. doi: 10.1006/meth.2001.1262
[32]	TEGEDER M, MASCLAUX-DAUBRESSE C. Source and sink mechanisms of nitrogen transport and use. New Phytologist, 2018,217(1):35-53. doi: 10.1111/nph.14876. doi: 10.1111/nph.14876
[33]	LIN S H, KUO H F, CANIVENC G, LIN C S, LEPETIT M, HSU P K, TILLARD P, LIN H L, WANG Y Y, TSAI C B, GOJON A, TSAY Y F. Mutation of the Arabidopsis NRT1.5 nitrate transporter causes defective root-to-shoot nitrate transport. The Plant Cell, 2008,20(9):2514-2528. doi: 10.1105/tpc.108.060244. doi: 10.1105/tpc.108.060244
[34]	LI J Y, FU Y L, PIKE S M, BAO J, TIAN W, ZHANG Y, CHEN C Z, ZHANG Y, LI H M, HUANG J, LI L G, SCHROEDER J I, GASSMANN W, GONG J M. The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance. The Plant Cell, 2010,22(5):1633-1646. doi: 10.1105/tpc.110.075242. doi: 10.1105/tpc.110.075242
[35]	吴宇. 低氮诱导的水稻根系形态解剖结构变化及其对氮素吸收分配的影响[D]. 武汉: 华中农业大学, 2020.
	WU Y. Low nitrogen induced changes of morphological and anatomical characterics and its effects on nitrogen uptake and distribution in rice[D]. Wuhan: Huazhong Agricultural University, 2020. (in Chinese)
[36]	岳慧芳, 任永哲, 李乐, 王新博, 吕伟增, 龚璞, 王志强, 辛泽毓, 林同保. 不同基因型小麦品种根系形态对低氮胁迫的响应. 河南农业大学学报, 2017,51(4):447-452. doi: 10.16445/j.cnki.1000-2340.2017.04.001. doi: 10.16445/j.cnki.1000-2340.2017.04.001
	YUE H F, REN Y Z, LI L, WANG X B, LÜ W Z, GONG P, WANG Z Q, XIN Z Y, LIN T B. The response of root morphological traits to low nitrogen stress in different genotypic wheat cultivars. Journal of Henan Agricultural University, 2017,51(4):447-452. doi: 10.16445/j.cnki.1000-2340.2017.04.001. (in Chinese) doi: 10.16445/j.cnki.1000-2340.2017.04.001
[37]	魏海燕, 张洪程, 张胜飞, 杭杰, 戴其根, 霍中洋, 许轲, 马群, 张庆, 刘艳阳. 不同氮利用效率水稻基因型的根系形态与生理指标的研究. 作物学报, 2008,34(3):429-436. doi: 10.3321/j.issn:0496-3490.2008.03.013. doi: 10.3321/j.issn:0496-3490.2008.03.013
	WEI H Y, ZHANG H C, ZHANG S F, HANG J, DAI Q G, HUO Z Y, XU K, MA Q, ZHANG Q, LIU Y Y. Root morphological and physiological characteristics in rice genotypes with different N use efficiencies. Acta Agronomica Sinica, 2008,34(3):429-436. doi: 10.3321/j.issn:0496-3490.2008.03.013. (in Chinese) doi: 10.3321/j.issn:0496-3490.2008.03.013
[38]	唐伟杰, 官春云, 林良斌, 李丽萍, 张振华, 王峰, 肖钢, 李博, 刘屹湘. 不同硝铵比对油菜生长、生理与产量的影响. 植物营养与肥料学报, 2018,24(5):1338-1348. doi: 10.11674/zwyf.17495. doi: 10.11674/zwyf.17495
	TANG W J, GUAN C Y, LIN L B, LI L P, ZHANG Z H, WANG F, XIAO G, LI B, LIU Y X. Effects of nitrate and ammonium supply ratios on growth, physiology and yield of oilseed rape (Brassica napus L.). Journal of Plant Nutrition and Fertilizers, 2018,24(5):1338-1348. doi: 10.11674/zwyf.17495. (in Chinese) doi: 10.11674/zwyf.17495
[39]	李生秀. 中国旱地土壤植物氮素. 北京: 科学出版社, 2008.
	LI S X. Soil and Plant Nitrogen in Dryland Areas of China. Beijing: Science Press, 2008. (in Chinese)
[40]	LI Q, DING G D, YANG N M, WHITE P J, YE X S, CAI H M, LU J W, SHI L, XU F S. Comparative genome and transcriptome analysis unravels key factors of nitrogen use efficiency in Brassica napus L. Plant, Cell & Environment, 2020,43(3):712-731. doi: 10.1111/pce.13689. doi: 10.1111/pce.13689
[41]	VERA J S, SPERANDIO M V L, FERNANDES M S, SANTOS L A. Overexpression of rice genes OsNRT1.1A and OsNRT1.1B restores chlorate uptake and NRT2.1/NAR2.1 expression in Arabidopsis thaliana chl1-5 mutant. Journal of Plant Growth Regulation, 2021,40(4):1701-1713. doi: 10.1007/s00344-020-10219-9. doi: 10.1007/s00344-020-10219-9
[42]	HU B, WANG W, OU S J, TANG J Y, LI H, CHE R H, ZHANG Z H, CHAI X Y, WANG H R, WANG Y Q, LIANG C Z, LIU L C, PIAO Z Z, DENG Q Y, DENG K, XU C, LIANG Y, ZHANG L H, LI L G, CHU C C. Variation in NRT1.1B contributes to nitrate-use divergence between rice sub species. Nature Genetics, 2015,47(7):834-838. doi: 10.1038/ng.3337. doi: 10.1038/ng.3337
[43]	HO C H, LIN S H, HU H C, TSAY Y F. CHL1 functions as a nitrate sensor in plants. Cell, 2009,138(6):1184-1194. doi: 10.1016/j.cell.2009.07.004. doi: 10.1016/j.cell.2009.07.004
[44]	ZHENG Y, DRECHSLER N, RAUSCH C, KUNZE R. The Arabidopsis nitrate transporter NPF7.3/NRT1.5 is involved in lateral root development under potassium deprivation. Plant Signaling & Behavior, 2016,11(5):e1176819. doi: 10.1080/15592324.2016.1176819. doi: 10.1080/15592324.2016.1176819
[45]	ZHOU Y, BAI L, SONG C P. Ammonium homeostasis and signaling in plant cells. Science Bulletin, 2015,60(8):741-747. doi: 10.1007/s11434-015-0759-2. doi: 10.1007/s11434-015-0759-2
[46]	LOQUÉ D, YUAN L X, KOJIMA S, GOJON A, WIRTH J, GAZZARRINI S, ISHIYAMA K, TAKAHASHI H, VON WIRÉN N. Additive contribution of AMT1;1 and AMT1;3 to high-affinity ammonium uptake across the plasma membrane of nitrogen-deficient Arabidopsis roots. The Plant Journal, 2006,48(4):522-534. doi: 10.1111/j.1365-313x.2006.02887.x. doi: 10.1111/j.1365-313x.2006.02887.x
[47]	YUAN L X, LOQUÉ D, KOJIMA S, RAUCH S, ISHIYAMA K, INOUE E, TAKAHASHI H, VON WIRÉN N. The organization of high-affinity ammonium uptake in Arabidopsis roots depends on the spatial arrangement and biochemical properties of AMT1-type transporters. The Plant Cell, 2007,19(8):2636-2652. doi: 10.1105/tpc.107.052134. doi: 10.1105/tpc.107.052134
[48]	GIEHL R F H, LAGINHA A M, DUAN F Y, RENTSCH D, YUAN L X, VON WIRÉN N. A critical role of AMT2;1 in root-to-shoot translocation of ammonium in Arabidopsis. Molecular Plant, 2017,10(11):1449-1460. doi: 10.1016/j.molp.2017.10.001. doi: 10.1016/j.molp.2017.10.001
[49]	LIMA J E, KOJIMA S, TAKAHASHI H, VON WIRÉN N. Ammonium triggers lateral root branching in Arabidopsis in an AMMONIUM TRANSPORTER1;3-dependent manner. The Plant Cell, 2010,22(11):3621-3633. doi: 10.1105/tpc.110.076216. doi: 10.1105/tpc.110.076216
[50]	YUAN L X, GRAFF L, LOQUÉ D, KOJIMA S, TSUCHIYA Y N, TAKAHASHI H, VON WIRÉN N. AtAMT1;4, a pollen-specific high-affinity ammonium transporter of the plasma membrane in Arabidopsis. Plant and Cell Physiology, 2008,50(1):13-25. doi: 10.1093/pcp/pcn186. doi: 10.1093/pcp/pcn186
[51]	ZHONG L H, HUANG X M, ZHU Y N, KOU E F, LIU H C, SUN G W, CHEN R Y, SONG S W. Characterization and expression analysis of BcAMT1;4, an ammonium transporter gene in flowering Chinese cabbage. Horticulture, Environment, and Biotechnology, 2019,60(4):563-572. doi: 10.1007/s13580-019-00155-3. doi: 10.1007/s13580-019-00155-3

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物候生长期 Phenological growth stages	处理 Treatment	种质 Germplasm	氮浓度 N concentration (%)		氮累积量 N accumulation (mg/plant)			氮转运效率 NTE (%)	氮吸收效率 NAE (%)	氮利用效率 NutE (%)
物候生长期 Phenological growth stages	处理 Treatment	种质 Germplasm	Shoot	Root	Shoot	Root	Total	氮转运效率 NTE (%)	氮吸收效率 NAE (%)	氮利用效率 NutE (%)
12	CK	498	5.55a	4.74a	1.26ab	0.24b	1.50ab	84.03c	3.00c	18.5c
	CK	428	5.63a	4.65a	1.39a	0.18b	1.57ab	88.51a	3.15c	18.2d
	LN	498	5.05b	4.40b	1.35ab	0.33a	1.68a	80.34d	67.45a	20.38b
	LN	428	4.93b	4.02c	1.12b	0.18b	1.30b	86.38b	51.96b	20.91a
14	CK	498	5.47a	4.90a	16.39a	2.35a	18.74a	87.39a	7.83c	18.55d
	CK	428	5.05b	5.05a	13.33b	2.15a	15.48a	86.04a	6.47c	19.80c
	LN	498	3.45c	3.44b	7.81c	1.89ab	9.70b	80.55b	81.05a	28.96b
	LN	428	3.16d	3.44b	6.25c	1.46b	7.70b	81.03b	64.36b	31.11a
16	CK	498	5.06b	4.65a	52.48a	9.10a	61.58a	85.16a	14.88c	20.02c
	CK	428	5.34a	4.81a	40.61b	6.79b	47.41b	85.64a	11.46d	19.03d
	LN	498	2.87c	2.22b	11.32c	4.60c	15.92c	71.02b	76.97a	37.78b
	LN	428	2.74c	2.26b	8.71d	3.22d	11.94d	72.93b	57.69b	38.56a

Characteristics of Efficient Nitrogen Uptake and Transport of Rapeseed at Seedling Stage

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