Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (24): 4793-4807.doi: 10.3864/j.issn.0578-1752.2022.24.002


Dynamic Changes of Gibberellin Content During the Development and Its Relationship with Yield of Brassica napus L.

XIE LingLi1,2(),WEI DingYi1,2(),ZHANG ZiShuang1,2,XU JinSong1,3,ZHANG XueKun1,3(),XU BenBo1,2()   

  1. 1Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou 434025, Hubei
    2College of Life Science, Yangtze University, Jingzhou 434025, Hubei
    3College of Agriculture, Yangtze University, Jingzhou 434025, Hubei
  • Received:2022-07-31 Accepted:2022-09-05 Online:2022-12-16 Published:2023-01-04
  • Contact: XueKun ZHANG,BenBo XU;;;


【Objective】It was found that there were significant genetic differences between Chinese and European Brassica napus lines, and the yield advantage of hybrid F1 was obvious. To explore the effect of gibberellin (GA) on the growth and development of B. napus, the dynamic changes of GA during the developmental process of B. napus from different lines were measured. For explaining the role of GA in the formation of yield, the transcriptional levels of key enzyme genes involved in GA synthesis were analyzed, and the relationship between GA content and yield in parents and F1 was clarified. 【Method】The dynamic changes of GA content in B. napus lines (15 Chinese lines and 15 European lines) with different sources from November 2020 to May 2021were determined using high performance liquid chromatography. Two F1hybrid lines (YG2009×YC4, ZS11×YC4) with strong heterosis and their parents were used as materials to investigate the changes of GA content in different periods (D1, 15 January 2022; D2, 15 February 2022; D3, 15 March 2022)and measure growth indicators (plant height, root length, fresh weight, etc.), yield and component factors (silique number per plant, seed number per silique and thousand-seed weight), as well as photosynthetic indicators (photosynthetic rate, stomatal conductance, transpiration rate and intercellular CO2 concentration).The change trend of GA content with temperature and the relationship between GA content and agronomic characters, photosynthetic characteristics and yield of parents and F1hybrid from November 2021 to May 2022 were analyzed. Besides, the transcriptional levels of key enzyme genes involved in GA synthesis of the parents and F1 at different stages (D1, D2, D3) also examined by quantitative real-time PCR. 【Result】The content of GA was closely related to the ambient temperature, and the GA content in the tested B. napus lines decreased first and then increased with the change of temperature. However, it was found that the average content of GA in B. napus lines from Europe was higher than that of Chinese lines. F1 showed significant yield heterosis. Compared with the male and female parents, the yield of YG2009×YC4 and ZS11×YC4 increased by 18.06% and 10.35%, and 29.92% and 28.6%, respectively. There were significant differences in agronomic traits between parents and F1, and the yield was correlated with GA content. The results also showed that the transcriptional levels of GA20ox4, GA3ox2 and SLR1 in parents and hybrid F1 varied greatly. 【Conclusion】The GA content of B. napus lines from Europe was more sensitive to temperature changes, but its average content of GA was higher than that from China. The yield of F1 between European rape and Chinese rape lines showed strong heterosis, which was correlated with GA content. The transcriptional levels of GA20ox4 and GA3ox2 regulated the GA content.

Key words: Brassica napus L., heterosis, gibberellins, yield, transcription level

Table 1

Primer sequences for qRT-PCR"

基因名称 Gene names 正向引物 Forward primer (5′-3′) 反向引物 Reverse primer (5′-3′)

Fig. 1

Daily temperature at test site from October 1, 2020 to June 1, 2021"

Fig. 2

Dynamic changes of GA during the whole growth period in B. napus"

Table 2

Analysis of yield and yield components of parents and hybrid F1"

Effective pods per plant
Seeds per pod
1000-seed weight (g)
Yield per plant (g)
Yield (kg·hm-2)
YG2009 268.75±16.39a 19.50±0.65c 3.79±0.01d 19.85±0.22c 2967.30±5.77c
ZS11 174.75±2.39b 20.25±0.48bc 4.66±0.02a 16.85±0.08d 2785.80±8.66d
YC4 164.75±5.54b 20.50±0.29abc 4.52±0.02b 15.30±0.11e 2757.60±2.89e
YG2009×YC4 255.50±11.70a 21.25±0.25ab 4.28±0.01c 22.20±0.19b 3255.60±3.26b
ZS11×YC4 273.25±11.45a 21.50±0.65a 4.32±0.01c 25.35±0.14a 3582.60±6.06a


Daily temperature at test site from October 1, 2021 to June 1, 2022"

Table 3

Analysis of agronomic traits of parents and F1 hybrid"

Plant height (cm)
Leaf number
Leaf length (cm)
Leaf width (cm)
Root length (cm)
Fresh weight (g)
Dry weight (g)
D1 YG2009 52.0±1.5b 10.3±0.3b 18.7±1.1ab 15.1±0.5a 15.1±0.9a 202.6±5.3b 20.2±0.3b
ZS11 57.2±0.6a 11.7±0.3a 16.5±0.8b 13.7±1.3ab 14.4±0.7a 196.5±21.3b 18.6±2.3b
YC4 40.5±1.5c 10.7±0.7ab 13.9±0.7c 12.6±0.6b 17.0±1.2a 147.7±6.4c 19.5±0.5b
YG2009×YC4 57.5±0.6a 11.7±0.3a 20.2±0.5a 15.5±0.2a 18.6±1.6a 264.3±8.6a 31.1±0.4a
ZS11×YC4 59.4±0.3a 10.0±0.0b 21.0±0.3a 15.9±0.2a 17.3±2.1a 192.8±11.8b 18.4±1.2b
D2 YG2009 57.5±1.3b 10.0±0.6bc 20.2±0.7b 16.7±0.4b 18.0±0.6b 254.1±3.0b 30.0±0.6b
ZS11 69.5±0.9a 12.0±0.6a 22.2±1.3ab 14.3±0.4c 18.1±0.3b 415.9±8.5a 53.0±2.6a
YC4 45.3±1.5c 9.0±0.6c 16.6±1.0c 13.93±1.0c 20.0±0.6a 112.3±6.2d 18.4±0.4c
YG2009×YC4 60.3±1.5b 12.0±0.6a 24.3±0.7a 19.7±0.7a 20.2±0.4a 254.9±3.2b 32.3±0.4b
ZS11×YC4 69.3±0.9a 11.0±0.6ab 21.2±0.6b 16.5±0.3b 19.2±0.4ab 233.9±4.1c 28.8±0.6b
D3 YG2009 83.3±3.8b 17.0±0.6c 21.0±0.6d 19.2±0.4b 19.0±0.6d 390.9±5.0d 66.8±2.3d
ZS11 111.3±1.9a 28.7±0.9a 24.0±0.6c 19.5±0.3ab 21.3±0.3cd 1234.9±4.0a 159.6±2.4a
YC4 47.3±1.8c 14.3±0.3d 17.0±0.6e 15.0±0.6c 22.7±0.9bc 163.6±4.5e 24.4±2.8e
YG2009×YC4 89.0±0.6b 17.3±0.9c 26.7±0.9b 21.5±1.0a 26.0±0.6a 536.8±2.7c 121.3±1.9c
ZS11×YC4 118.0±1.15a 21.7±0.3b 30.0±0.6a 21.0±0.6ab 24.3±1.2ab 881.9±5.8b 142.4±3.3b
方差分析Variance analysis
YG2009×YC4 ** ** * NS NS ** NS * NS NS ** ** ** **
ZS11×YC4 NS ** ↓* NS ** ** NS * NS NS NS * NS NS
D2 YG2009×YC4 NS ** * ** ** ** ** ** * NS NS ** NS **
ZS11×YC4 NS ** NS * NS ** * * NS NS ↓** ** ↓** **
D3 YG2009×YC4 NS ** NS ** ** ** * ** ** * ** ** ** **
ZS11×YC4 NS ** ↓** ** ** ** NS ** * NS ↓** ** ↓** **

Fig. 4

Analysis of photosynthetic characteristics of parents and F1 hybrid Different lowercase letters indicated a difference of 0.05 levels. The same as below"

Fig. 5

Analysis of GA dynamic changes in parents and hybrid F1"

Fig. 6

Relative transcriptional levels of key enzyme genes in GA synthesis pathway at different stages"

Fig. 7

Relative expression levels of GA metabolic key genes in parents and hybrid F1 at different developmental stages a: Change of relative transcriptional levels=Relative transcriptional levels on February 15, 2022 - relative transcriptional levels on January 15, 2022; b: Change of transcriptional levels =Relative transcriptional levels on March 15, 2022 - relative transcriptional levels on February 15, 2022"

Fig. 8

GAcontent in parents and hybrids F1seedlingsin plant growth room"

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