Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (19): 4027-4034.doi: 10.3864/j.issn.0578-1752.2013.19.008

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Study on Correlation Between Ammonia Volatilization and Nitrogen Metabolism During the Tobacco Leaves Senescence

 WU  Yun-Jie, YANG  Tie-Zhao, ZHANG  Xiao-Quan, LI  Fei, LI  Li-Hua   

  1. College of Tobacco, Henan Agricultural University, Zhengzhou 450002
  • Received:2013-03-06 Online:2013-10-01 Published:2013-07-04

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Abstract: 【Objective】The objective of this experiment is to study the difference of different N efficiency varieties of flue-cured tobacco leaf senescence ammonia volatilization amount in the process, reveal tobacco varieties N transport law, so as to provide a basis for nitrogen resistant variety breeding.【Method】Using ammonia collection device was used to determine the amount of ammonia volatilization of tobacco. At the same time, leaves NH4+ concentration, total N and soluble protein content, N metabolism-related enzyme activity, and apoplast related indicators were measured.【Result】Tobacco amount of ammonia volatilization showed a trend of first increase and then decrease, the maximum at leaf age of 60 d. The amount in varieties Zhongyan90, K326 and NC89, respectively, reached (16.39±1.08) μg•m-2•h-1, (9.92±1.04) μg•m-2•h-1, and (4.56±0.63) μg•m-2•h-1, respectively, 3.87, 2.41 and 2.56 times of the leaf age 40 d ammonia volatilization, and the difference among the varieties was significant. The amount of ammonia volatilization and glutamine synthetase (GS) activity were significantly and negatively correlated, and very significantly and positively correlated with the glutamate dehydrogenase (GDH) activity, apoplastic NH4+ concentration and pH, ammonia compensation point, while very significantly and negatively correlated, with the content of soluble protein and nitrate reductase (NR) activity, They showed a quadratic curve with the leaves NH4+ concentration, and the correlation was extremely significant, and are subject to total N degradation of indirect control. 【Conclusion】 There is a difference among different nitrogen efficient varieties in nitrogen reassimilation and transfer ability，and it is associated with tobacco leaf N metabolism characteristics. During the tobacco senescence, varieties of low N use efficiency, N absorption and assimilation capacity is weak, the transfer capacity, ammonia volatilization amount is big. Through volatilization of ammonia outside the body, increased the transfer of NH4+, reduced the accumulation of ammonia pollution, so it is an important method for plant N metabolic regulation, which can be used as an evaluation of index of a variety nitrogen efficiency.

Key words: flue-tobacco , ammonia volatilization , N metabolism , NH3 compensation point

[1]刘青丽, 任天志, 李志宏, 石俊雄, 张恒, 张云贵. 植烟黄壤供氮特征研究. 中国农业科学, 2010, 43(1): 87-95.

Liu Q L, Ren T Z, Li Z H, Shi J X, Zhang H, Zhang Y G. The characteristics of nitrogen supply in yellow soil planted with tobacco. Scientia Agricultura Sinica, 2010, 43(1): 87-95. (in Chinese)

[2]李春俭, 张福锁, 李文卿, 赵正雄, 习向银, 石秋梅, 晁逢春, 路永宪, 陶芾, 秦燕青, 巨晓棠, 江荣风. 我国烤烟生产中的氮素管理及其与烟叶品质的关系. 植物营养与肥料学报, 2007, 13(2): 331-337.

Li C J, Zhang F S, Li W Q, Zhao Z X, Xi X Y, Shi Q M, Chao F C, Lu Y X, Tao F, Qin Y Q, Ju X T, Jiang R F. Nitrogen management and its relation to leaf quality in production of flue-cured tobacco. Plant Nutrition and Fertilizer Science, 2007, 13(2): 331-337. (in Chinese)

[3]Sommer S G, Schjoerring J K, Denmead O T. Ammonia emission from mineral fertilizers and fertilized crops. Advances in Agronomy, 2004, 82: 557-622.

[4]Herrmann B, Mattsson M, Jones S K, Cellier P, Milford C, Sutton M A, Schjoerring J K, Neftel A. Vertical structure and diurnal variability of ammonia exchange potential within an intensively managed grass canopy. Biogeosciences, 2009, 6: 15-23.

[5]Husted S, Schjoerring J K. Ammonia flux between oilseed rape plants and the atmosphere in response to changes in leaf temperature, light intensity and air humidity. Plant Physiology, 1996, 112: 67-74.

[6]Massad R S, Loubet B, Tuzet A, Cellier P. Relationship between ammonia stomatal compensation point and nitrogen metabolism in arable crops: Current status of knowledge and potential modeling approaches. Environmental Pollution, 2008, 154: 390-403.

[7]陈刚, 徐阳春, 沈其荣. 施氮水平对水稻生育后期地上部氨挥发的影响. 应用生态学报, 2008, 19(7): 1483-1488.

Chen G, Xu C Y, Shen Q R. Effects on fertilization levels on ammonia volatilization from rice shoot at later growth stages. Chinese Journal of Applied Ecology, 2008, 19(7): 1483-1488. (in Chinese)

[8]徐阳春, 吴小庆, 郭世伟, 沈其荣. 水稻生育后期地上部氨挥发与氮素利用效率的研究. 植物营养与肥料学报, 2008, 14(2): 207-212.

Xu C Y, Wu X Q, Guo S W, Shen Q R. Nitrogen use efficiency and ammonia volatilization from rice shoot in late growth stages. Plant Nutrition and Fertilizer Science, 2008, 14(2): 207-212. (in Chinese)

[9]Sreeramamuthy C H, Arishukumar P H, Nageswararao C R. Change in concentration of nitrogenous constituents in flue-cured tobacco leaf as affected by nitrogen fertilization in vertisols. Tobacco Research, 1996, 22(1): 22-25.

[10]岳红宾. 不同氮素水平对烟草碳氮代谢关键酶活性的影响. 中国烟草科学, 2007, 28(1): 18-24.

Yue H B. Effects of various nitrogen levels on key enzymes activeness of flue-cured tobacco leaves in carbon and nitrogen metabolism. Chinese Tobacco Science, 2007, 28(1): 18-24. (in Chinese)

[11]武云杰, 张小全, 段旺军, 杨铁钊. 不同氮素利用效率基因型烤烟叶片衰老期间氮素代谢差异研究. 中国烟草学报, 2012, 18(5): 42-47.

Wu Y J, Zhang X Q, Duan W J, Yang T Z. Different in N metabolism in flue-cure tobacco genotypes with differential N intake efficiency during leaf senescence. Acta Tabacaria Sinica, 2012, 18(5): 42-47. (in Chinese)

[12]吴小庆, 徐阳春, 沈其荣, 郭世伟. 不同氮肥利用效率水稻品种开花后地上部分氨挥发研究. 中国水稻科学, 2006, 20(4): 429-433.

Wu X Q, Xu Y C, Shen Q R, Guo S W. Ammonia volatilization from shoots of different rice genotypes with different nitrogen use efficiency after flowering. Chinese Journal of Rice Science, 2006, 20(4): 429-433. (in Chinese)

[13]陈明霞, 黄见良, 崔克辉, 聂立孝, 彭少兵. 不同氮效率基因型水稻植株氨挥发速率及其与氮效率的关系. 作物学报, 2010, 36(5): 879-884.

Chen M X, Huang J L, Cui K H, Nie L X, Peng S B. Genotypic variation in ammonia volatilization rate of rice shoots and its relationship with nitrogen use efficiency. Acta Agronomica Sinica, 2010, 36(5): 879-884. (in Chinese)

[14]Wang Z H, Li S X. NH3 volatilization from aboveground plants of winter wheat during late growing stages. Agricultural Science in China, 2003, 2(4): 384-393.

[15]Mattsson M, Schjoerring J K. Dynamic and steady-state responses of inorganic nitrogen pools and NH3 exchange in leaves of Lolium perenne and Bromus erectus to changes in root supply. Plant Physiology, 2002, 128: 742-750.

[16]Mattsson M, Herrmann B, Jones S, Neftel A, Sutton M A, Schjoerring J K. Contribution of different grass species to plant-atmosphere ammonia exchange in intensively managed grassland. Biogeosiences Discuss, 2008, 5: 2583-2605.

[17]刘化冰, 杨铁钊, 张小全, 段旺军, 尚晓颍. 不同耐肥烤烟品种质外体NH4+浓度差异和有关指标分析. 中国农业科学, 2010, 43(14): 3036-3043.

Liu H B, Yang T Z, Zhang X Q, Duan W J, Shang X Y. Difference of apoplastic NH4+ concentration of flue-cured tobacco varieties with different tolerance to nitrogen nutrition and assay of the related physiological parameters. Scientia Agricultura Sinica, 2010, 43(14): 3036-3043. (in Chinese)

[18]段旺军, 杨铁钊, 刘化冰, 张小全, 戴亚, 李东亮. 烟叶氨气补偿点的品种间差异及其与氮素代谢的关系研究. 植物营养与肥料学报, 2011, 17(2): 149-158.

Duan W J, Yang T Z, Liu H B, Zhang X Q, Dai Y, Li D L. Differences in NH3 compensation point among tobacco (Nicotiana tabacum L.) cultivars and its relationship with nitrogen metabolism. Plant Nutrition and Fertilizer Science, 2011, 17(2): 149-158. (in Chinese)

[19]武云杰, 张小全, 段旺军, 杨铁钊. 烤烟叶片衰老期氨气挥发特征及其生理调控研究. 西北植物学报, 2012, 32(9): 2082-2088.

Wu Y J, Zhang X Q, Duan W J, Yang T Z. Ammonia volatilization characteristics and its physiological regulation in flue-cured tobacco leaves during senescence. Acta Botanica Boreali-Occidentalia Sinica, 2012, 32(9): 2082-2088. (in Chinese)

[20]O’Neal D, Joyk W. Glutamine synthetase of pea leaves. I. Purification, stabilization, and pH optima. Archives of Biochemistry and Biophysics, 1973, 159: 113-122.

[21]Turano F J, Dashner R, Upadhyaya A, Caldwell C R. Purification of mitochondrial glutamate dehydrogenase from dark-grown soybean seedlings. Plant Physiology, 1996, 112: 1357-1364.

[22]邹琦. 植物生理学实验指导. 北京: 中国农业出版社, 2000: 56-129.

Zou Q. The Guidance of Plant Physiology Experiment. Beijing: China Agriculture Press, 2000: 56-129. (in Chinese)

[23]Schjoerring J K, Husted S, Mäck G, Nielsen K H, Finnemann J, Mattsson. Physiological regulation of plant-atmosphere ammonia exchange. Plant and Soil, 2000, 221: 95-101.

[24]Foyer C H, Notor G, Lelandais M, Lescure J C, Valadier N H, Boution J P, Horton P. Short-term effects of nitrate, nitrite and ammonium assimilation and amino biosynthesis in maize. Planta, 1994, 192: 21l-220.

[25]Hortensteiner S, Feller U. Nitrogen metabolism and remobilization during senescence. Journal of Experimental Botany, 2002, 53: 927-937.

[26]Eichelmann H, Laisk A. Rubulose-1,5-bisphosphate carboxylase/ oxygenase content, assimilatory charge, and mesophyll conductance in leaves. Plant Physiology, 199: 179-189.

[27]Masclaux-Daubresse C, Reisdorf-Cren M, Pageau K. Glutamine synthetase-glutamate synthase pathway and glutamate dehydrogenase play distinct roles in the sink-source nitrogen cycle in tobacco. Plant Physiology, 2006, 140: 444-456.

[28]Masclaux, Valadier M H, Brugiere N. Characterization of the sink/source transition in tobacco (Nicotiana tabacum L.) shoots in relation to nitrogen management and leaf senescence. Planta, 2000, 211: 510-518.

[29]Brugiere N, Dubois F, Masclaux C, Sangwan R S, Hirel B. Immunolocalization of glutamine synthetase in senescing tobacco (Nicotiana tabacum L.) leaves suggests that ammonia assimilation is progressively shifted to the mesophyll cytosol. Planta, 2000, 211: 519-527.

[30]Hausler R E, Blackwell R D, Lea P J, Leegood R C. Control of photosynthesis in barley leaves with reduced activities of glutamine synthetase or glutamate synthase. I. Plant characteristics and changes in nitrate, ammonium and amino acids. Planta, 1994, 194: 406-417.

[31]Mattsson M, Schjoerring J K. Senescence-induced changes in apoplastic and bulk tissue ammonia concentrations of ryegrass leaves. New Phytologist, 2003, 160(11): 489-499.

[32]Cosgrove D J, Cleland R E. Solutes in the free space of growing tissues. Plant Physiology, 1983, 72: 326-331.

[33]吴小庆, 徐阳春, 沈其荣. 植物叶片氨挥发研究进展. 生态与农村环境学报, 2006, 22(2): 80-84.

Wu X Q, Xu C Y, Shen Q R. progress in research on ammonia volatilization from plant leaves. Journal of Ecology and Rural Environment, 2006, 22(2): 80-84. (in Chinese)
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