Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (13): 2625-2632.doi: 10.3864/j.issn.0578-1752.2013.13.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Effects of OsLTP Gene on Salt Tolerance of Transgenic Brassica napus

 DU  Kun, GAO  Ya-Nan, KONG  Yue-Qin, FAN  Yun, WANG  You-Ping   

  1. College of Bioscience and Biotechnology of Yangzhou University, Yangzhou 225009, Jiangsu
  • Received:2013-02-01 Online:2013-07-01 Published:2013-05-13

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Abstract: 【Objective】 This study aimed to understand the function of OsLTP gene under salt stress. 【Method】 An efficient expression vector of pCAM2300-35S-LTP-Ocs harboring OsLTP gene from Brazilian upland rice was transferred into Brassica napus cv. Yangyou 6 via Agrobacterium-mediated genetic transformation of the rapeseed hypocotyls. 【Result】The integration of the foreign OsLTP gene was confirmed by PCR and southern hybridization analysis. In the treatments of 100 mmol•L-1 and 200 mmol•L-1 NaCl solution, the biomass of plants, chlorophyll accumulation, PSⅡ activity, and antioxidant enzyme activities in leaves of the transgenic plants were higher than that of the non-transformants, MDA content in leaves was insteadly lower than the controls. 【Conclusion】 OsLTP gene improved salt tolerance of transgenic B. napus by maintaining PSII activity, chlorophyll accumulation in leaves and increased the activities of antioxidant enzymes.

Key words: Brassica napus L. , OsLTP , genetic transformation , salt stress

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