Table 1

Primers used in the study"

Fig. 1

The differential expression ratio of the transcriptome and proteome of the two phosphoethanolamine N-methyltransferases genes GhPEAMT1A and GhPEAMT1D at 4 h and 24 h after salt stress A: Differentially expressed genes; B: Differential abundance protein. E: Earlistape 7; N: Nandanbadidahua; 4: 200 mmol·L-1 NaCl treatment for 4 h; 24: 200 mmol·L-1 NaCl treatment for 24 h. There were no biological replicates in transcriptome data, but three in proteome data"

Fig. 2

GhPEAMT1 gene cloning and analysis of conserved domain A: GhPEAMT1A clone electrophoresis; B: GhPEAMT1D clone electrophoresis; C: Analysis of conserved domain of GhPEAMT1 protein; M1: Trans2K? Plus II Marker; 1&3: Earlistable 7; 2&4: Nandanbadidahua"

Fig. 3

Amino acid sequence alignment and phylogenetic tree analysis for gene GhPEAMT1 A: Multiple sequence alignment of GhPEAMT1. Gbar_A03G010420: Gossypium barbadense, XM_016811336; Gbar_D02G013090: Gossypium barbadense, XM_016835662; Thecc1EG012768t1: Theobroma cacao, XM_007036740; Gorai.005G132000: Gossypium raimondii, XM_012623073; Gh_A03G0803: Gossypium hirsutum, GhPEAMT1A; Gh_D02G1225: Gossypium hirsutum, GhPEAMT1D; AT1G48600: Arabidopsis thaliana, NP_973993.1; AT3G18000: Arabidopsis thaliana, NP_188427.2; LOC_Os01g47540: Oryza sativa, XM_015785333; LOC_Os01g50030: Oryza sativa, XP_015622327; Ga03G1288: Gossypium arboreum, XM_017755899; Potri.012G047400: Populus trichocarpa, XM_024582451; GRMZM2G122296: Zea mays, NP_001307897.2; The black line indicates the position of conserved domain; B: Phylogenetic analysis of GhPEAMT1; The black box indicates the target gene"

Fig. 4

Analysis of the expression characteristics of GhPEAMT1 gene in cotton tissues A: The expression of GhPEAMT1A gene in the leaves of Earlistape 7 and Nandanbadidahua; B: Expression of GhPEAMT1A gene in roots of Earlistape 7 and Nandanbadidahua; C: Expression of GhPEAMT1D gene in leaves of Earlistape 7 and Nandanbadidahua; D: GhPEAMT1D gene in early expression in roots of Earlistape 7 and Nandanbadidahua. E: Earlistape 7; N: Nandanbadidahua"

Fig. 5

GhPEAMT1A and GhPEAMT1D gene subcellular localization"

Fig. 6

Obtaining transgenic Arabidopsis A: PCR detection transgenic Arabidopsis plants GhPEAMT1A, M: Marker; 1-16: Transgenic plants; B: PCR detection transgenic Arabidopsis plants GhPEAMT1D, C: Expression and identification of GhPEAMT1 in transgenic Arabidopsis plants; WT: Wild type Arabidopsis; OE1-OE6: Transgenic Arabidopsis plants"

Fig. 7

Effect of Salt Stress on seedling growth in Arabidopsis A-D: Germination rate of Arabidopsis under NaCl treatment of 0, 50, 100 and 150 mmol·L-1; E-H: Root elongation of Arabidopsis thaliana treated with 0, 50, 100 and 150 mmol·L-1 NaCl; I: Germination rate (%) of Arabidopsis under different NaCl concentrations; J: Main root length of Arabidopsis under different NaCl concentration. *: Significant difference (P<0.05), **: Extremely significant difference (P<0.01). The same as below"

Fig. 8

GhPEAMT1 gene silencing efficiency and phenotype after salt treatment A: Silence marker gene TRV2: CLA plants; B: Analysis of GhPEAMT1 gene silencing efficiency after virus-induced gene silencing, abscissas 1-7: Strains injected with TRV2::GHPEAMT1A, TRV2::GhPEAMT1D strains; C-D: 48 h after salt stress, CK, TRV2:00, TRV2::GHPEAMT1A, TRV2::GHPEAMT1D phenotype of cotton young plants"

Fig. 9

Determination of enzyme activity after GhPEAMT1 gene silencing treatment A: The active of CAT in TRV2:00, TRV2::GHPEAMT1A cotton seedlings after 48 h of salt stress; B: The active of GPX in TRV2:00, TRV2::GHPEAMT1A cotton seedlings after 48 h of salt stress"