中国春小麦肌醇磷脂依赖的磷脂酶C基因的全基因组鉴定及表达分析

doi:10.3864/j.issn.0578-1752.2020.24.001

Abstract

Abstract:

【Objective】To characterize the gene family encoding phosphatidylinositol-specific phospholipase-C (PI-PLC), the gene number, structure, phylogenetic relationship and expression pattern of TaPLC genes were analyzed. In addition, we explored their relative transcription levels in various tissue and the expression pattern under drought and salt stress to further analysis the physiological role of TaPLC genes in response to abiotic stress.【Method】Based on the genome database (Ensembl Plants, http://plants.ensembl.org/index.html), TaPLC genes were identified from Triticum aestivum, and the gene structures were analyzed by some bioinformatics tools, such as Pfam, CDD and SMART. The protein sequence characteristics of the members in TaPLC family were analyzed with the online server ExPASy (http://cn.expasy.org/tools). The subcellular localization of TaPLC genes were predicted using the WoLF PSORT (https://www.genscript.com/wolf-psort.html). Phylogenic tree analysis was performed by software MEGA 7.0. The real-time PCR was used to detect the gene expression levels in different tissues under abiotic stress. 【Result】A total of 11 TaPLC genes members within the heterologous hexaploid wheat genome were identified and analyzed. All TaPLC genes have conserved X-Y catalytic domains and C2 domains, just like other plant PLC genes. Subcellular localization showed that these proteins were located in the chloroplast or mitochondria. Phylogenetic analysis revealed that all of the TaPLC genes were unevenly classified into four numbered, TaPLC1A/TaPLC1D, TaPLC2A/TaPLC2B/TaPLC2D, TaPLC3A/TaPLC3B/TaPLC3D, and TaPLC4A/TaPLC4B/TaPLC4D, which were all orthologous genes of plant PLC genes in plants, and highly conserved during evolution. However, the deletion of TaPLC1 in B subgenome indicates asymmetry evolution in different subgenomes. Further analysis of cis-regulatory elements elucidated that these members of TaPLC family shared similar elements, such as the phytohormone and the stress response cis-elements. Each PLC gene has a unique tissue expression pattern, and they are expressed mainly in roots, leaves, spikes, and gains. In addition, PLC genes mediate the response of wheat to salt and drought stresses, which are induced rapidly by salt or drought stress stimulation. 【Conclusion】The TaPLC family in Triticum aestivum contains 11 members which have conserved X-Y catalytic domains and C2 domains. Although the TaPLC genes were highly conserved, they were asymmetrically evolved in different subgenomes. They had specific expression pattern in different tissues, and were induced by salt or drought stress, which suggested TaPLC genes play a role in response to salt or drought in wheat.

Key words: Triticum aestivum, PI-PLC, salt stress, drought stress

SI XuYang,JIA XiaoWei,ZHANG HongYan,JIA YangYang,TIAN ShiJun,ZHANG Ke,PAN YanYun. Genomic Profiling and Expression Analysis of Phosphatidylinositol- specific PLC Gene Families Among Chinese Spring Wheat[J].Scientia Agricultura Sinica, 2020, 53(24): 4969-4981.

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References 43

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基因名称 Gene name	基因ID Gene ID	染色体定位^a Chromosomal location	开放阅读框 Open reading frame (bp)	蛋白质^b Protein
基因名称 Gene name	基因ID Gene ID	染色体定位^a Chromosomal location	开放阅读框 Open reading frame (bp)	大小 Size (aa)	分子量 MW (kD)	等电点 pI
TaPLC1A	TraesCS1A02G069300	1A:51700021—51707185	1758	585	66.01	6.07
TaPLC1D	TraesCS1D02G071800	1D:52338417—52345805	1761	586	66.19	6.03
TaPLC2A	TraesCS2A02G084000	2A:38534860—38538480	1830	609	68.5	5.91
TaPLC2B	TraesCS2B02G098500	2B:58321242—58325230	1821	606	68.22	6.06
TaPLC2D	TraesCS2D02G082000	2D:35259471—35263395	1824	607	68.45	5.88
TaPLC3A	TraesCS4A02G109000	4A:129086595—129090166	1902	633	71.11	5.54
TaPLC3B	TraesCS4B02G195200	4B:420197892—420201623	1902	633	71.00	5.75
TaPLC3D	TraesCS4D02G195800	4D:340085748—340090648	1902	633	71.05	5.84
TaPLC4A	TraesCS5A02G155300	5A:333407514—333413175	1773	590	65.73	6.05
TaPLC4B	TraesCS5B02G153600	5B:283008744—283014326	1770	589	65.65	6.06
TaPLC4D	TraesCS5D02G160300	5D:250061407—250066699	1770	589	65.65	6.06

基因Gene	亚细胞定位Subcellular localization
TaPLC1A	线粒体：8,叶绿体：4,细胞核：1 Mito: 8, chlo: 4, nucl: 1
TaPLC1D	线粒体：8,叶绿体：4,细胞核：1 Mito: 8, chlo: 4, nucl: 1
TaPLC2A	叶绿体：7,细胞核：3,线粒体：2.5,线粒体基质：2 Chlo: 7, nucl: 3, mito: 2.5, cyto_mito: 2
TaPLC2B	叶绿体：6,线粒体：5.5,线粒体基质：3.5,细胞核：1 Chlo: 6, mito: 5.5, cyto_mito: 3.5, nucl: 1
TaPLC2D	叶绿体：7,线粒体：4.5,线粒体基质：3,细胞核：1 Chlo: 7, mito: 4.5, cyto_mito: 3, nucl: 1
TaPLC3A	细胞质：8,细胞核：4,叶绿体：1 Cyto: 8, nucl: 4, chlo: 1
TaPLC3B	细胞质：8,细胞核：3,叶绿体：1,线粒体：1 Cyto: 8, nucl: 3, chlo: 1, mito: 1
TaPLC3D	细胞质：6,细胞核：3,线粒体：2,过氧化物：2 Cyto: 6, nucl: 3, mito: 2, pero: 2
TaPLC4A	线粒体：10,叶绿体和线粒体：6.83333,线粒体基质：5.83333,叶绿体：2.5 Mito: 10, chlo_mito: 6.83333, cyto_mito: 5.83333, chlo: 2.5
TaPLC4B	线粒体：10,叶绿体和线粒体：6.83333,线粒体基质：5.83333,叶绿体：2.5 Mito: 10, chlo_mito: 6.83333, cyto_mito: 5.83333, chlo: 2.5
TaPLC4D	线粒体：10,叶绿体和线粒体：6.83333,线粒体基质：5.83333,叶绿体：2.5 Mito: 10, chlo_mito: 6.83333, cyto_mito: 5.83333, chlo: 2.5

Genomic Profiling and Expression Analysis of Phosphatidylinositol- specific PLC Gene Families Among Chinese Spring Wheat

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