Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (21): 4573-4584.doi: 10.3864/j.issn.0578-1752.2021.21.008

• PLANT PROTECTION • Previous Articles     Next Articles

Effect of Bacillus subtilis NCD-2 on the Growth of Tomato and the Microbial Community Structure of Rhizosphere Soil Under Salt Stress

SHAO MeiQi1,2(),ZHAO WeiSong2,SU ZhenHe2,DONG LiHong2,GUO QingGang2,*(),MA Ping2   

  1. 1College of Plant Protection, Hebei Agricultural University, Baoding 071001, Hebei
    2Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences/IPM Centre of Hebei Province/Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, Baoding 071000, Hebei
  • Received:2021-03-17 Accepted:2021-03-31 Online:2021-11-01 Published:2021-11-09
  • Contact: QingGang GUO;


【Objective】The objective of this study is to evaluate the growth promotion effect of Bacillus subtilis strain NCD-2 on tomato seedlings under salt stress, as well as the effect of strain NCD-2 on soil microbial community diversity. The results will be useful for expanding the application of strain NCD-2 in agricultural system.【Method】The pot experiments were conducted to evaluate the effects of strain NCD-2 treatment on stem length, aboveground fresh weight, aboveground dry weight, root fresh weight and root dry weight. The activity of resistance-related enzymes such as peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and the content of abscisic acid (ABA) were measured. High-throughput sequencing (Illumina MiSeq) technique was used to determine the bacterial and fungal community structures in the rhizosphere soil. The four treatments were set as NCD-2 strain suspension treatment (NCD0), 100 mmol·L -1 NaCl treatment (CK100), NCD-2 strain suspension + 100 mmol·L-1 NaCl treatment (NCD100) and water treatment as control (CK0).【Result】Under normal conditions, strain NCD-2 treatment significantly increased the biomass of tomato. The plant height, aboveground fresh weight, aboveground dry weight, root fresh weight and root dry weight were increased by 9.08%, 10.37%, 16.64%, 15.42% and 16.78%, respectively, when compared with the control. Under salt stress, compared to the control, the plant height, aboveground fresh weight, aboveground dry weight, root fresh weight and root dry weight were increased by 16.86%, 18.96%, 21.32%, 10.50% and 23.99% after treated with strain NCD-2, respectively. The activity of resistance-related enzymes SOD, POD, CAT and the content of ABA were increased by 50.45%, 56.18%, 29.55% and 34.60% after treated with strain NCD-2, respectively, when compared with the control. For bacteria community composition analysis, compared to CK0, the relative abundance of bacteria phylum Actinobacteria, Acidobacteria and Chloroflexi was increased by 7.28%, 15.14% and 23.03% after treated with strain NCD-2 without salt stress, respectively. The relative abundance of bacteria genus Arthrobacter, Sphingomonas, Microvirga and Streptomyces was increased by 50.88%, 15.31%, 11.32% and 16.41% after treated with strain NCD-2, respectively. Under 100 mmol·L -1 NaCl stress, compared to CK100, the relative abundance of bacteria phylum Proteobacteria, Actinobacteria, Firmicutes and Gemmatimonadetes was increased by 6.08%, 8.19%, 14.11% and 4.70% after treated with strain NCD-2, respectively. The relative abundance of bacteria genus Arthrobacter, Bacillus, Sphingomonas and Microvirga was increased by 5.54%, 31.80%, 23.39% and 23.08% after treated with strain NCD-2, respectively. For fungal community composition analysis, compared to CK0, the relative abundance of fungal phylum Mortierellomycota, Glomeromycota and Chytridiomycota was increased to 186%, 477% and 1 650% of CK0, respectively. The relative abundance of fungal genus Mortierella, Trichoderma and Preussia was increased to 186%, 108%, and 120% of CK0 after treated with strain NCD-2 without salt stress, respectively. Under 100 mmol·L -1 NaCl stress, compared to CK100, the relative abundance of fungal phylum Mortierellomycota, Glomeromycota and Chytridiomycota was increased to 345%, 154%, 921% of CK100 after treated with strain NCD-2, respectively. The relative abundance of fungal genus Mortierella was increased by 246% after treated with strain NCD-2.【Conclusion】After the treatment of strain NCD-2 under salt stress, the activity of stress-resistant enzymes and the content of ABA in tomato were increased, and the population of beneficial microorganisms in tomato rhizosphere was increased, thus improving the tolerance of tomato to salt stress and significantly increasing the growth and development of tomato.

Key words: Bacillus subtilis, salt stress, activity of antioxidant enzymes, high-throughput sequencing, soil microbial community structure

Fig. 1

Effects of NCD-2 strain on tomato growth under NaCl stress"

Fig. 2

Activity of antioxidant enzymes and ABA content in tomato"

Fig. 3

PCOA analysis of bacterial community structure"

Fig. 4

Changes of relative abundance of bacterial community composition at phylum level"

Fig. 5

Changes of relative abundance of bacterial community composition at genus level"

Fig. 6

PCOA analysis of fungus community structure"

Fig. 7

Changes of relative abundance of fungal community composition at phylum level"

Fig. 8

Changes of relative abundance of fungal community composition at genus level"

[1] MANGAT P K, GANNABAN R B, SINGLETON J J, ANGELES- SHIM R B. Development of a PCR-based, genetic marker resource for the tomato-like nightshade relative, Solanum lycopersicoides using whole genome sequence analysis. PLoS ONE, 2020, 15(11):e0242882.
doi: 10.1371/journal.pone.0242882
[2] MAYAK S, TIROSH T, GLICK B R. Plant growth-promoting bacteria confer resistance in tomato plants to salt stress. Plant Physiology and Biochemistry, 2004, 42(6):565-572.
doi: 10.1016/j.plaphy.2004.05.009
[3] GAMALERO E, BERTA G, MASSA N, GLICK B R, LINGUA G. Interactions between Pseudomonas putida UW4 and Gigaspora rosea BEG9 and their consequences for the growth of cucumber under salt -stress conditions. Journal of Applied Microbiology, 2010, 108(1):236-245.
doi: 10.1111/jam.2009.108.issue-1
[4] NUMAN M, BASHIR S, KHAN Y, MUMTAZ R, SHINWARI Z K, KHAN A L, KHAN A, AL-HARRASI A. Plant growth promoting bacteria as an alternative strategy for salt tolerance in plants: A review. Microbiological Research, 2018, 209:21-32.
doi: 10.1016/j.micres.2018.02.003
[5] SHARMA S, KULKARNI J, JHA B. Halotolerant rhizobacteria promote growth and enhance salinity tolerance in peanut. Frontiers in Microbiology, 2016, 7:1600.
[6] PAUL D, LADE H. Plant-growth-promoting rhizobacteria to improve crop growth in saline soils: A review. Agronomy for Sustainable Development, 2014, 34(4):737-752.
doi: 10.1007/s13593-014-0233-6
[7] SARKAR A, GHOSH P K, PRAMANIK K, MITRA S, SOREN T, PANDEY S, MONDAL M H, MAITI T K. A halotolerant Enterobacter sp. displaying ACC deaminase activity promotes rice seedling growth under salt stress. Research in Microbiology, 2018, 169(1):20-32.
doi: 10.1016/j.resmic.2017.08.005
[8] 牛舒琪. 梭梭根际促生细菌调控黑麦草生长和抗逆性的生理研究[D]. 兰州: 兰州大学, 2017.
NIU S Q. Physiological research on growth promoting rhizobacteria from Haloxylon ammodendron regulating perennial ryegrass growth and stress tolerance[D]. Lanzhou: Lanzhou University, 2017. (in Chinese)
[9] EL-ESAWI M, ALARAIDH I, ALSAHLI A, ALZAHRANI S, ALI H, ALAYAFI A, AHMAD M. Serratia liquefaciens KM4 improves salt stress tolerance in maize by regulating redox potential, ion homeostasis, leaf gas exchange and stress-related gene expression. International Journal of Molecular Sciences, 2018, 19(11):3310.
doi: 10.3390/ijms19113310
[10] ZHOU C, MA Z, ZHU L, XIAO X, XIE Y, ZHU J, WANG J. Rhizobacterial strain Bacillus megaterium BOFC15 induces cellular polyamine changes that improve plant growth and drought resistance. International Journal of Molecular Sciences, 2016, 17(6):976.
doi: 10.3390/ijms17060976
[11] YASMEEN T, AHMAD A, ARIF M S, MUBIN M, REHMAN K, SHAHZAD S M, IQBAL S, RIZWAN M, ALI S, ALYEMENI M N, WIJAYA L. Biofilm forming rhizobacteria enhance growth and salt tolerance in sunflower plants by stimulating antioxidant enzymes activity. Plant Physiology and Biochemistry, 2020, 156:242-256.
doi: 10.1016/j.plaphy.2020.09.016
[12] LATEF A A, OMER A M, BADAWY A A, OSMAN M S, RAGAEY M M. Strategy of salt tolerance and interactive impact of Azotobacter chroococcum and/or Alcaligenes faecalis inoculation on canola (Brassica napus L.) plants grown in saline soil. Plants, 2021, 10(1):110.
doi: 10.3390/plants10010110
[13] 赵丹丹. 不同浓度微生物菌剂对番茄土壤理化性质及生长的影响[D]. 杨凌: 西北农林科技大学, 2020.
ZHAO D D. Effects of different concentrations of microbial agents on physical and chemical properties and growth of tomato soil[D]. Yangling: Northwest A&F University, 2020. (in Chinese)
[14] BUDDRUS-SCHIEMANN K, SCHMID M, SCHREINER K, WELZL G, HARTMANN A. Root colonization by Pseudomonas sp. DSMZ 13134 and impact on the indigenous rhizosphere bacterial community of barley. Microbial Ecology, 2010, 60(2):381-393.
doi: 10.1007/s00248-010-9720-8
[15] GAMALERO E, LINGUA G, TOMBOLINI R, AVIDANO L, PIVATO B, BERTA G. Colonization of tomato root seedling by Pseudomonas fluorescens 92rkG5: Spatio-temporal dynamics, localization, organization, viability, and culturability. Microbial Ecology, 2005, 50(2):289-297.
doi: 10.1007/s00248-004-0149-9
[16] 王小慧, 张国漪, 李蕊, 卢颖林, 冉炜, 沈其荣. 拮抗菌强化的生物有机肥对西瓜枯萎病的防治作用. 植物营养与肥料学报, 2013, 19(1):223-231.
WANG X H, ZHANG G Y, LI R, LU Y L, RAN W, SHEN Q R. Control of watermelon fusarium wilt by using antagonist-enhanced biological organic fertilizers. Journal of Plant Nutrition and Fertilizer, 2013, 19(1):223-231. (in Chinese)
[17] 黄亚丽, 郑立伟, 黄媛媛, 贾振华, 宋水山, 李再兴. 枯草芽孢杆菌菌剂不同施用方式对甜瓜土壤微生物多样性及生长的影响. 生物工程学报, 2020, 36(12):2644-2656.
HUANG Y L, ZHENG L W, HUANG Y Y, JIA Z H, SONG S S, LI Z X. Effects of different application methods of Bacillus subtilis agent on soil microbial diversity and growth of muskmelon. Chinese Journal of Biotechnology, 2020, 36(12):2644-2656. (in Chinese)
[18] 邱勤, 张磊, 韩光, 石杰, 胡正峰. 使用PGPR菌剂及苜蓿培肥新垦地土壤研究. 西南大学学报 (自然科学版), 2011, 33(5):109-115.
QIU Q, ZHANG L, HAN G, SHI J, HU Z F. Improving fertility of newly reclaimed soil by PGPR inoculums in combination with alfalfa growing. Journal of Southwest University (Natural Science Edition), 2011, 33(5):109-115. (in Chinese)
[19] GIANNOPOLITIS C N, RIES S K. Superoxide dismutases: II. Purification and quantitative relationship with water-soluble protein in seedlings. Plant Physiology, 1977, 59(2):315-318.
doi: 10.1104/pp.59.2.315
[20] 曾韶西, 王以柔. 不同胁迫预处理提高水稻抗寒期间膜保护系统的变化比较. 植物学报, 1997, 39(4):308-314.
ZENG S X, WANG Y R. Comparison of the changes of membrane protection system in rice seedlings during enhancement of chilling resistance by different stress pretreatments. Acta Botanica Sinica, 1997, 39(4):308-314. (in Chinese)
[21] DHINDSA R S, PLUMB-DHINDSA P, THORPE T A. Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 1981, 32(126):93-101.
doi: 10.1093/jxb/32.1.93
[22] ARKHIPOVA T N, PRINSEN E, VESELOV S U, MARTINENKO E V, MELENTIEV A I, KUDOYAROVA G R. Cytokinin producing bacteria enhance plant growth in drying soil. Plant and Soil, 2007, 292:305-315.
doi: 10.1007/s11104-007-9233-5
[23] XU N, TAN G, WANG H, GAI X. Effect of biochar additions to soil on nitrogen leaching, microbial biomass and bacterial community structure. European Journal of Soil Biology, 2016, 74:1-8.
doi: 10.1016/j.ejsobi.2016.02.004
[24] ADAMS R I, MILETTO M, TAYLOR J W, BRUNS T D. Dispersal in microbes: Fungi in indoor air are dominated by outdoor air and show dispersal limitation at short distances. The ISME Journal, 2013, 7(7):1262-1273.
doi: 10.1038/ismej.2013.28
[25] MELONI D A, OLIVA M A, MARTINEZ C A, CAMBRAIA J. Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environmental and Experimental Botany, 2003, 49(1):69-76.
doi: 10.1016/S0098-8472(02)00058-8
[26] KIM K, JANG Y J, LEE S M, OH B T, CHAE J C, LEE K J. Alleviation of salt stress by Enterobacter sp. EJ01 in tomato and Arabidopsis is accompanied by up-regulation of conserved salinity responsive factors in plants. Molecules and Cells, 2014, 37(2):109-117.
doi: 10.14348/molcells.2014.2239
[27] SAMADDAR S, CHATTERJEE P, CHOUDHURY A R, AHMED S, SA T. Interactions between Pseudomonas spp. and their role in improving the red pepper plant growth under salinity stress. Microbiological Research, 2019, 219:66-73.
doi: 10.1016/j.micres.2018.11.005
[28] KHAN M H, PANDA S K. Alterations in root lipid peroxidation and antioxidative responses in two rice cultivars under NaCl-salinity stress. Acta Physiologiae Plantarum, 2008, 30:81-89.
doi: 10.1007/s11738-007-0093-7
[29] BHARTI N, PANDEY S S, BARNAWAL D, PATEL V K, KALRA A. Plant growth promoting rhizobacteria Dietzia natronolimnaea modulates the expression of stress responsive genes providing protection of wheat from salinity stress. Scientific Reports, 2016, 6:34768.
doi: 10.1038/srep34768
[30] MAUCH-MANI B, FLORS V. The ATAF1 transcription factor: At the convergence point of ABA-dependent plant defense against biotic and abiotic stresses. Cell Research, 2009, 19(12):1322-1323.
doi: 10.1038/cr.2009.135
[31] TAO Z, KOU Y, LIU H, LI X, XIAO J, WANG S. OsWRKY45 alleles play different roles in abscisic acid signalling and salt stress tolerance but similar roles in drought and cold tolerance in rice. Journal of Experimental Botany, 2011, 62(14):4863-4874.
doi: 10.1093/jxb/err144
[32] 郑娜, 柯林峰, 杨景艳, 王雪飞, 黄典, 程万里, 李嘉晖, 郑龙玉, 喻子牛, 张吉斌. 来源于污染土壤的植物根际促生细菌对番茄幼苗的促生与盐耐受机制. 应用与环境生物学报, 2018, 24(1):47-52.
ZHENG N, KE L F, YANG J Y, WANG X F, HUANG D, CHENG W L, LI J H, ZHENG L Y, YU Z N, ZHANG J B. Growth improvement and salt tolerance mechanisms of tomato seedlings mediated by plant growth-promoting rhizobacteria from contaminated soils. Chinese Journal of Applied and Environmental Biology, 2018, 24(1):47-52. (in Chinese)
[33] HE Z Q, HE C X, ZHANG Z B, ZOU Z R, WANG H S. Changes of antioxidative enzymes and cell membrane osmosis in tomato colonized by arbuscular mycorrhizae under NaCl stress. Colloids and Surfaces B: Biointerfaces, 2007, 59(2):128-133.
doi: 10.1016/j.colsurfb.2007.04.023
[34] BAKKER M G, CHAPARRO J M, MANTER D K, VIVANCO J M. Impacts of bulk soil microbial community structure on rhizosphere microbiomes of Zea mays. Plant and Soil, 2015, 392:115-126.
doi: 10.1007/s11104-015-2446-0
[35] WALTERS W A, JIN Z, YOUNGBLUT N, WALLACE J G, SUTTER J, ZHANG W, GONZALEZ-PENA A, PEIFFER J, KOREN O, SHI Q, et al. Large-scale replicated field study of maize rhizosphere identifies heritable microbes. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(28):7368-7373.
[36] BERENDSEN R L, PIETERSE C, BAKKER P. The rhizosphere microbiome and plant health. Trends in Plant Science, 2012, 17(8):478-486.
doi: 10.1016/j.tplants.2012.04.001
[37] 樊祖清, 芦阿虔, 王海涛, 王岩, 段宏群, 李宏丽, 邱光. 施用解淀粉芽孢杆菌对烟株生长和根际土壤微生物区系的影响. 河南农业科学, 2019, 48(4):33-40.
FAN Z Q, LU A Q, WANG H T, WANG Y, DUAN H Q, LI H L, QIU G. Effects of Bacillus amyloliquefaciens on the growth of tobacco and microflora in rhizosphere soil. Journal of Henan Agricultural Science, 2019, 48(4):33-40. (in Chinese)
[38] QIAO J, YU X, LIANG X, LIU Y, BORRISS R, LIU Y. Addition of plant-growth-promoting Bacillus subtilis PTS-394 on tomato rhizosphere has no durable impact on composition of root microbiome. BMC Microbiology, 2017, 17(1):131.
doi: 10.1186/s12866-017-1039-x
[39] GUO Q, LI S, LU X, LI B, MA P. PhoR/PhoP two component regulatory system affects biocontrol capability of Bacillus subtilis NCD-2. Genetics and Molecular Biology, 2010, 33(2):333-340.
doi: 10.1590/S1415-47572010005000032
[40] 朱伟杰, 王楠, 郁雪平, 王伟. 生防菌Pseudomonas fluorescens 2P24对甜瓜根围土壤微生物的影响. 中国农业科学, 2010, 43(7):1389-1396.
ZHU W J, WANG N, YU X P, WANG W. Effects of the biocontrol agent Pseudomonas fluorescens 2P24 on microbial community diversity in the melon rhizosphere. Scientia Agricultura Sinica, 2010, 43(7):1389-1396. (in Chinese)
[41] ALI A, MOHANTA T K, ASAF S, REHMAN N, AL-HOUSNI S, AL-HARRASI A, KHAN A L, AL-RAWAHI A. Biotransformation of benzoin by Sphingomonas sp. LK11 and ameliorative effects on growth of Cucumis sativus. Archives of Microbiology, 2019, 201:591-601.
doi: 10.1007/s00203-019-01623-1
[42] EL-LAITHY N A, BADAWY E A, YOUNESS E R, IBRAHIM A M, EL-NEMR M, EL-SHAMY K A. Antioxidant defense system as a protector against oxidative stress induced by thyroid dysfunction. Der Pharmacia Lettre, 2016, 8(6):113-118.
[1] MA XueMeng,YU ChengMin,SAI XiaoLing,LIU Zhen,SANG HaiYang,CUI BaiMing. PSORA: A Strategy Based on High-Throughput Sequence for Analysis of T-DNA Insertion Sites [J]. Scientia Agricultura Sinica, 2022, 55(15): 2875-2882.
[2] HU YaLi,NIE JingZhi,WU Xia,PAN Jiao,CAO Shan,YUE Jiao,LUO DengJie,WANG CaiJin,LI ZengQiang,ZHANG Hui,WU QiJing,CHEN Peng. Effect of Salicylic Acid Priming on Salt Tolerance of Kenaf Seedlings [J]. Scientia Agricultura Sinica, 2022, 55(14): 2696-2708.
[3] ZHU ChunYan,SONG JiaWei,BAI TianLiang,WANG Na,MA ShuaiGuo,PU ZhengFei,DONG Yan,LÜ JianDong,LI Jie,TIAN RongRong,LUO ChengKe,ZHANG YinXia,MA TianLi,LI PeiFu,TIAN Lei. Effects of NaCl Stress on the Chlorophyll Fluorescence Characteristics of Seedlings of Japonica Rice Germplasm with Different Salt Tolerances [J]. Scientia Agricultura Sinica, 2022, 55(13): 2509-2525.
[4] GONG XiaoYa,SHI JiBo,FANG Ling,FANG YaPeng,WU FengZhi. Effects of Flooding on Soil Chemical Properties and Microbial Community Composition on Farmland of Continuous Cropped Pepper [J]. Scientia Agricultura Sinica, 2022, 55(12): 2472-2484.
[5] LIU Chuang,GAO Zhen,YAO YuXin,DU YuanPeng. Functional Identification of Grape Potassium Ion Transporter VviHKT1;7 Under Salt Stress [J]. Scientia Agricultura Sinica, 2021, 54(9): 1952-1963.
[6] ZHANG GuiYun,ZHU JingWen,SUN MingFa,YAN GuoHong,LIU Kai,WAN BaiJie,DAI JinYing,ZHU GuoYong. Analysis of Differential Metabolites in Grains of Rice Cultivar Changbai 10 Under Salt Stress [J]. Scientia Agricultura Sinica, 2021, 54(4): 675-683.
[7] DU Yu,ZHU ZhiWei,WANG Jie,WANG XiuNa,JIANG HaiBin,FAN YuanChan,FAN XiaoXue,CHEN HuaZhi,LONG Qi,CAI ZongBing,XIONG CuiLing,ZHENG YanZhen,FU ZhongMin,CHEN DaFu,GUO Rui. Construction and Annotation of Ascosphaera apis Full-Length Transcriptome Utilizing Nanopore Third-Generation Long-Read Sequencing Technology [J]. Scientia Agricultura Sinica, 2021, 54(4): 864-876.
[8] WANG Jie,WU XiaoYu,YANG Liu,DUAN QiaoHong,HUANG JiaBao. Genome-Wide Identification and Expression Analysis of ACA Gene Family in Brassica rapa [J]. Scientia Agricultura Sinica, 2021, 54(22): 4851-4868.
[9] ZHAO WeiSong,GUO QingGang,DONG LiHong,WANG PeiPei,SU ZhenHe,ZHANG XiaoYun,LU XiuYun,LI SheZeng,MA Ping. Transcriptome and Proteome Analysis of Bacillus subtilis NCD-2 Response to L-proline from Cotton Root Exudates [J]. Scientia Agricultura Sinica, 2021, 54(21): 4585-4600.
[10] WANG Na,ZHAO ZiBo,GAO Qiong,HE ShouPu,MA ChenHui,PENG Zhen,DU XiongMing. Cloning and Functional Analysis of Salt Stress Response Gene GhPEAMT1 in Upland Cotton [J]. Scientia Agricultura Sinica, 2021, 54(2): 248-260.
[11] HUANG ZiYue,LIU WenJun,QIN RenLiu,PANG ShiChan,XIAO Jian,YANG ShangDong. Endophytic Bacterial Community Composition and PICRUSt Gene Functions in Different Pumpkin Varieties [J]. Scientia Agricultura Sinica, 2021, 54(18): 4018-4032.
[12] KONG YaLi,ZHU ChunQuan,CAO XiaoChuang,ZHU LianFeng,JIN QianYu,HONG XiaoZhi,ZHANG JunHua. Research Progress of Soil Microbial Mechanisms in Mediating Plant Salt Resistance [J]. Scientia Agricultura Sinica, 2021, 54(10): 2073-2083.
[13] LI Hui,HAN ZhanPin,HE LiXia,YANG YaLing,YOU ShuYan,DENG Lin,WANG ChunGuo. Cloning and Functional Analysis of BraERF023a Under Salt and Drought Stresses in Cauliflower (Brassica oleracea L. var. botrytis) [J]. Scientia Agricultura Sinica, 2021, 54(1): 152-163.
[14] CHEN LuLu,WANG Hui,WANG JiKun,WANG JiaBo,CHAI ZhiXin,CHEN ZhiHua,ZHONG JinCheng. Comparative Analysis of miRNA Expression Profiles in the Hearts of Tibetan Cattle and Xuanhan Cattle [J]. Scientia Agricultura Sinica, 2020, 53(8): 1677-1687.
[15] HaiYan ZHANG,BeiTao XIE,BaoQing WANG,ShunXu DONG,WenXue DUAN,LiMing ZHANG. Effects of Drought Treatments at Different Growth Stages on Growth and the Activity of Antioxidant Enzymes in Sweetpotato [J]. Scientia Agricultura Sinica, 2020, 53(6): 1126-1139.
Full text



No Suggested Reading articles found!