Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (14): 2696-2708.doi: 10.3864/j.issn.0578-1752.2022.14.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Effect of Salicylic Acid Priming on Salt Tolerance of Kenaf Seedlings

HU YaLi(),NIE JingZhi,WU Xia,PAN Jiao,CAO Shan,YUE Jiao,LUO DengJie,WANG CaiJin,LI ZengQiang,ZHANG Hui,WU QiJing,CHEN Peng()   

  1. College of Agriculture, Guangxi University/Guangxi Colleges and Universities Key Laboratory of Plant Genetics and Breeding, Nanning 530004
  • Received:2022-02-27 Accepted:2022-04-24 Online:2022-07-16 Published:2022-07-26
  • Contact: Peng CHEN E-mail:2541764968@qq.com;chenmanuscript@163.com

Abstract:

【Objective】To study the growth and physiological response of salicylic acid (SA) priming in kenaf under salt stress, and further reveal the induction pattern of SA priming on the stress-related genes in kenaf, thus provide a theoretical basis for salt tolerance study in kenaf. 【Method】Two different salt-tolerant kenaf cultivars (resistant and sensitive cultivars codenamed CP018 and CP047, respectively) were used as materials. The seeds were tested by SA priming and then subjected to hydroponics experiments to analyze the effect of SA priming on kenaf seed germination and the agronomic and physiological aspects of seedling under 150 mmol·L-1 NaCl stress, and the expression patterns of SA priming stress-related genes were analyzed by qRT-PCR. 【Result】The germination rate, germination potential and germination index of the salt-resistant cultivar CP018 were significantly improved after 0.2 mmol·L-1 SA priming, by 34.78%, 31.30% and 58.07%, respectively; the salt-susceptible cultivar CP047 also showed some improvement, by 7.50%, 10.56% and 6.23%, respectively, but did not reached the significant level. Under salt stress conditions, plant height inhibition was significantly reduced by 4.07% (CP018) and 3.91% (CP047) in the 2 cultivars by SA priming (S1) compared with un-priming (N1), and dry weight inhibition was significantly reduced by 15.50% (CP018) and 15.68% (CP047), in the 2 cultivars, respectively; fresh weight inhibition was significantly reduced by 4.46% in CP047, but not in CP018. Analysis of the root systems showed that root length inhibition was significantly reduced by 10.74% (CP018) and 10.77% (CP047) in the two cultivars, respectively, root surface area inhibition decreased by 5.09% (CP018) and 2.95% (CP047) in the two cultivars, reaching a significant level only in the salt-resistant cultivar CP018, while root activity inhibition was significantly reduced by 46.21% in the salt-susceptible cultivar CP047 and 6.56% in the salt-resistant cultivar CP018, reaching a significant level only in the salt-susceptible cultivar CP047. A grey correlation analysis of the indicators revealed that root activity was the most relevant factor influencing plants dry weight. SA priming reduced the MDA content and increased the POD and SOD enzyme activities of kenaf leaves under salt stress. Expression analysis of 12 stress-related genes showed that ACCD, APX2, SOS1, ARR2, PAL, CHIT and TIFY11 genes expression levels were significantly up-regulated after SA priming, while ERF9, ERS1, ERF.C3 and MYC2 and XTH22 expression patterns differed between the two cultivars, with XTH22 being significantly up-regulated in salt sensitive cultivar CP047 but not in the salt resistant cultivar CP018, ERS1 and MYC2 were significantly up-regulated in the salt resistant cultivar CP018 but significantly down regulated in the salt sensitive cultivar CP047, while the trend of ERF9 was opposite in the two cultivars. 【Conclusion】SA priming at a suitable concentration could significantly alleviate the growth of kenaf under salt stress, and differed in the degree and patterns of effects on different kenaf germplasm resources. SA may regulate kenaf plant response to abiotic stresses by affecting physiological processes such as antioxidant enzyme systems and mediating the expression of specific genes.

Key words: kenaf (Hibiscus cannabinus L.), seed priming, salicylic acid, antioxidant enzyme activity, salt stress

Table 1

Settings for various treatment combinations"

处理 Treatment CP018 CP047
S0 0.2 mmol·L-1 SA 0.2 mmol·L-1 SA
S1 0.2 mmol·L-1 SA +150 mmol·L-1 NaCl 0.2 mmol·L-1 SA +150 mmol·L-1 NaCl
N0 - -
N1 150 mmol·L-1 NaCl 150 mmol·L-1 NaCl

Table 2

Primer sequences for qRT-PCR"

引物名称
Primer name
正向引物
Forward primer (5′-3′)
反向引物
Reverse primer (5′-3′)
18SrRNA (reference gene) AGAAACGGCTACCACATC TACTCATTCCAATTACCAGACTC
ERF9 GCGTGAGTTTCGTGGACATAAG CCTTCCACCGTCGGGCTA
APX2 ACCCCACAGTGAGCGAAGATT GCTAAACGGAGGATGATGGG
ACCD AGAGGCAGGCGAAGGAAGC GAGAGTCCCCAGGAGAGCGA
XTH22 CAACTCTGCTGGCACTGTCACTG TCTGTTACCCTTTCCTTGGCTGAAC
TIFY11 CCAAAAGTCCGGCAAGGCTCTC GCCTAGATGTTTCAATCCCTGCAAC
ERS1 GAAGCACACTCGACAGGCACAC TCCACAGGGCACATTCCTCCAG
MYC2 TCTTCAGCCTCCTCCTCATCGC CCAGAATATAGCATACGCCCACCAG
ARR2 AGGCTTCTCAAGACTTCTTCGGTTG AGATTCACTCCCAGTCCCACATCC
SOS1 CGGAGCCTGTTGATGCGGTTATC CCGAGACCGATGCCAATGATGAG
CHIT ATGTCGGCAGCAAAACTTCA TGCTGGGGCTCATCTCCTT
PAL TGGCCACGACCCTTTGAACT CCGAAACTCAGCCACCATGC
ERF.C3 AGGTCCAGGGGCAAGAAATGTAAAC TCCCAAATCCTCAAACACCACCAAG

Fig. 1

Effects of SA Seed Priming on seed germination of kenaf Different letters in the treatments represent significant differences at the 0.05 level. The same as below"

Fig. 2

Seedling morphology of kenaf after SA seed priming under salt stress"

Fig. 3

Agronomic traits of kenaf after SA seed priming under salt stress"

Fig. 4

Root system scan of kenaf after SA seed priming under NaCl stress"

Fig. 5

Root system analysis of kenaf after SA seed priming under NaCl stress"

Fig. 6

Effect of SA seed priming on root activity of kenaf plants under NaCl stress"

Table 3

Grey correlation analysis of the indicators of SA seed priming in kenaf under salt stress"

指标
Index
根系活力
Root activity
鲜重
Fresh weight
株高
Plant height
根总长度
Root total length
根表面积
Root surface area
关联度 Correlation degree 0.689978 0.884894 0.900494 0.921566 0.892805
排序 Order 1 2 4 5 3

Fig. 7

Effect of SA seed priming on MDA content and antioxidant enzyme activity of kenaf leaves under NaCl stress"

Fig. 8

qRT-PCR detection of the stress-related genes"

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