水杨酸引发对红麻幼苗耐盐性的影响

doi:10.3864/j.issn.0578-1752.2022.14.002

Abstract

Abstract:

【Objective】To study the growth and physiological response of salicylic acid (SA) priming in kenaf under salt stress, and further reveal the induction pattern of SA priming on the stress-related genes in kenaf, thus provide a theoretical basis for salt tolerance study in kenaf. 【Method】Two different salt-tolerant kenaf cultivars (resistant and sensitive cultivars codenamed CP018 and CP047, respectively) were used as materials. The seeds were tested by SA priming and then subjected to hydroponics experiments to analyze the effect of SA priming on kenaf seed germination and the agronomic and physiological aspects of seedling under 150 mmol·L^-1 NaCl stress, and the expression patterns of SA priming stress-related genes were analyzed by qRT-PCR. 【Result】The germination rate, germination potential and germination index of the salt-resistant cultivar CP018 were significantly improved after 0.2 mmol·L^-1 SA priming, by 34.78%, 31.30% and 58.07%, respectively; the salt-susceptible cultivar CP047 also showed some improvement, by 7.50%, 10.56% and 6.23%, respectively, but did not reached the significant level. Under salt stress conditions, plant height inhibition was significantly reduced by 4.07% (CP018) and 3.91% (CP047) in the 2 cultivars by SA priming (S1) compared with un-priming (N1), and dry weight inhibition was significantly reduced by 15.50% (CP018) and 15.68% (CP047), in the 2 cultivars, respectively; fresh weight inhibition was significantly reduced by 4.46% in CP047, but not in CP018. Analysis of the root systems showed that root length inhibition was significantly reduced by 10.74% (CP018) and 10.77% (CP047) in the two cultivars, respectively, root surface area inhibition decreased by 5.09% (CP018) and 2.95% (CP047) in the two cultivars, reaching a significant level only in the salt-resistant cultivar CP018, while root activity inhibition was significantly reduced by 46.21% in the salt-susceptible cultivar CP047 and 6.56% in the salt-resistant cultivar CP018, reaching a significant level only in the salt-susceptible cultivar CP047. A grey correlation analysis of the indicators revealed that root activity was the most relevant factor influencing plants dry weight. SA priming reduced the MDA content and increased the POD and SOD enzyme activities of kenaf leaves under salt stress. Expression analysis of 12 stress-related genes showed that ACCD, APX2, SOS1, ARR2, PAL, CHIT and TIFY11 genes expression levels were significantly up-regulated after SA priming, while ERF9, ERS1, ERF.C3 and MYC2 and XTH22 expression patterns differed between the two cultivars, with XTH22 being significantly up-regulated in salt sensitive cultivar CP047 but not in the salt resistant cultivar CP018, ERS1 and MYC2 were significantly up-regulated in the salt resistant cultivar CP018 but significantly down regulated in the salt sensitive cultivar CP047, while the trend of ERF9 was opposite in the two cultivars. 【Conclusion】SA priming at a suitable concentration could significantly alleviate the growth of kenaf under salt stress, and differed in the degree and patterns of effects on different kenaf germplasm resources. SA may regulate kenaf plant response to abiotic stresses by affecting physiological processes such as antioxidant enzyme systems and mediating the expression of specific genes.

Key words: kenaf (Hibiscus cannabinus L.), seed priming, salicylic acid, antioxidant enzyme activity, salt stress

HU YaLi,NIE JingZhi,WU Xia,PAN Jiao,CAO Shan,YUE Jiao,LUO DengJie,WANG CaiJin,LI ZengQiang,ZHANG Hui,WU QiJing,CHEN Peng. Effect of Salicylic Acid Priming on Salt Tolerance of Kenaf Seedlings[J].Scientia Agricultura Sinica, 2022, 55(14): 2696-2708.

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References 44

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处理 Treatment	CP018	CP047
S0	0.2 mmol·L^-1 SA	0.2 mmol·L^-1 SA
S1	0.2 mmol·L^-1 SA +150 mmol·L^-1 NaCl	0.2 mmol·L^-1 SA +150 mmol·L^-1 NaCl
N0	-	-
N1	150 mmol·L^-1 NaCl	150 mmol·L^-1 NaCl

引物名称 Primer name	正向引物 Forward primer (5′-3′)	反向引物 Reverse primer (5′-3′)
18SrRNA (reference gene)	AGAAACGGCTACCACATC	TACTCATTCCAATTACCAGACTC
ERF9	GCGTGAGTTTCGTGGACATAAG	CCTTCCACCGTCGGGCTA
APX2	ACCCCACAGTGAGCGAAGATT	GCTAAACGGAGGATGATGGG
ACCD	AGAGGCAGGCGAAGGAAGC	GAGAGTCCCCAGGAGAGCGA
XTH22	CAACTCTGCTGGCACTGTCACTG	TCTGTTACCCTTTCCTTGGCTGAAC
TIFY11	CCAAAAGTCCGGCAAGGCTCTC	GCCTAGATGTTTCAATCCCTGCAAC
ERS1	GAAGCACACTCGACAGGCACAC	TCCACAGGGCACATTCCTCCAG
MYC2	TCTTCAGCCTCCTCCTCATCGC	CCAGAATATAGCATACGCCCACCAG
ARR2	AGGCTTCTCAAGACTTCTTCGGTTG	AGATTCACTCCCAGTCCCACATCC
SOS1	CGGAGCCTGTTGATGCGGTTATC	CCGAGACCGATGCCAATGATGAG
CHIT	ATGTCGGCAGCAAAACTTCA	TGCTGGGGCTCATCTCCTT
PAL	TGGCCACGACCCTTTGAACT	CCGAAACTCAGCCACCATGC
ERF.C3	AGGTCCAGGGGCAAGAAATGTAAAC	TCCCAAATCCTCAAACACCACCAAG

指标 Index	根系活力 Root activity	鲜重 Fresh weight	株高 Plant height	根总长度 Root total length	根表面积 Root surface area
关联度 Correlation degree	0.689978	0.884894	0.900494	0.921566	0.892805
排序 Order	1	2	4	5	3

Effect of Salicylic Acid Priming on Salt Tolerance of Kenaf Seedlings

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