Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (19): 4058-4065.doi: 10.3864/j.issn.0578-1752.2011.19.016

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Continuous Extraction of Saponin and Polysaccharide from Momordica charantia L. and Their Inhibitory Effect on α-glucosidase

 WANG  Qi, DENG  Yuan-Yuan, ZHANG  Ming-Wei, ZHANG  Rui-Fen, ZHANG  Yan, TANG  Xiao-Jun, WEI  Zhen-Cheng, CHI  Jian-Wei   

  1. 1.广东省农业科学院农业生物技术研究所/农业部功能食品重点开放实验室
  • Received:2010-10-13 Online:2011-10-01 Published:2011-07-13

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Abstract: 【Objective】 The technical process and suitable conditions for continuous extraction of saponin and polysaccharide from Momordica charantia L. by ultrasonic-microwave synergistic extraction were established and to evaluate their inhibition effects on α-glucosidase 【Method】 Quadratic Regression Rotational Combinational Design was applied to optimize the extraction process of saponin and polysaccharide from Momordica charantia L. using continuous ultrasonic-microwave synergistic extraction method. The method with substrate 4-nitrophenol-2-?-D-glucopyranoside(PNPG) was used to measure the α-glucosidase inhibitory effect of saponin and polysaccharide extract. 【Result】 The optimum conditions for saponin and polysaccharide extraction form Momordica charantia L. were as follows: the dried Momordica charantia L. with 13% of water content was grinded to pass a 200 μm sieve; the solvent was 75% enthanol and the ratio of material to solvent was 1:18; ultrasonic-microwave synergistic extraction for 16 min (the power of microwave was 50 W and the ultrasonic frequency was 20 kHz). Under this condition the extraction yield of saponin from Momordica charantia L. was 2.51%. The residue was further dissolved in water of pH 8.0 for extraction of polysaccharide, the ratio of material to solvent was 1:14; the ultrasonic-microwave synergetic extraction time was 23 min (the power of microwave was 50 W and the ultrasonic frequency was 20 kHz) at a temperature of 84℃. Under these conditions, the extraction yield of polysaccharide from Momordica charantia L. was 12.86%. Both of the extracts of saponin and polysaccharide from Momordica charantia L. showed an obvious inhibitory activity against α-glucosidase with values of 1.03 and 10.73 mg•mL-1, respectively.【Conclusion】 The raw materials were utilized effectively and the extraction yield and efficiency were increased using a method of ultrasonic-microwave synergistic extraction. The extracted saponin and polysacchride from Momordica charantia L. showed an obvious inhibition effect on α-glucosidase with promising of blood sugar reducing potentials.

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