蜂胶提取物对脂多糖诱导小鼠急性乳腺炎及乳腺屏障功能的保护作用

doi:10.3864/j.issn.0578-1752.2021.12.017

Abstract

Abstract:

【Background】 Dairy cow mastitis is a common and frequent dairy cow disease, which not only threatens the health of dairy cows, but also causes significant economic losses. The traditional medicine for the treatment of dairy cow mastitis is largely dependent on the usage of antibiotics, which easily caused antibiotic resistance/abuse. Therefore, it is with practical significance to develop an alternative approach of antibiotics for the prevention and treatment of mastitis in dairy cows. Propolis is a natural product with good anti-inflammatory and antibacterial activities. Propolis is collected by western honeybees from plant resin and mixing with bees’ maxillary gland and wax gland secretions. Accordingly, propolis has great potential for the prevention and treatment of mastitis in dairy cows. Despite that there have some previous reports on the prevention and treatment of mastitis using propolis, scant information is available on the effects of propolis on the breast blood barrier function. 【Objective】The aim of this study was to evaluate the protective effect of ethanol extract of Chinese propolis, EECP, against bacterial lipopolysaccharide- induced mouse acute mastitis, and we focused on its effect on the expression of tight junction proteins in mammary epithelial cells. This outcome of this study would lay a foundation for the in-depth study for the usage of propolis for prevention and treatment on dairy cow mastitis. 【Method】 The type and content of the main polyphenolic compounds in EECP were determined by ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS). Female ICR mice were divided into four groups, including the normal control group, model group (injecting LPS into the mammary gland, 1mg·kg^-1 b.w.), EECP group and positive control group (dexamethasone). The female mice were subjected to continuous gavage by EECP or positive control drugs for seven days. The LPS model group, EECP group and positive control group were injected with 1 mg·kg^-1 LPS through the fourth and the fifth pair of nipple tubes, to induce acute mastitis in mice. After 24 h, they were killed for collecting the mammary tissue samples. Hematoxylin-Eosin (HE) staining method and Sirius red staining method were used to evaluate pathological changes in mouse mammary tissue. Enzyme-linked immunosorbent assay (ELISA) was used to determine the release of inflammatory factors and quantitative real-time PCR was applied to determine mouse mammary tissue gene expressions of tight junction proteins (Occludin, Cluadin-1, ZO-1). Finally, the immunohistochemical technology was used to study the expression and distribution of mouse mammary tight junction proteins (Occludin and ZO-1). These above indicators were applied to comprehensively evaluate the anti-mastitis activity and its effect on mammary tight junction proteins by EECP. 【Result】 Based on UHPLC-QqQ-MS/MS, an accurate quantitative method was established for the 17 main polyphenolic compounds in EECP. The results showed that the five compounds with the higher content and their contents were: galangin (12.88 ±0.57 μg·mg^-1), 3-O-Acetylpinobanksin (12.93±0.59 μg·mg^-1), pinocembrin (8.56±0.27 μg·mg^-1) and pinobanksin (8.52±0.25 μg·mg^-1). Animal study results showed that after a week of regular oral administration of EECP reduced on the infiltrations of inflammatory cells in mammary gland under LPS stimulation, and the structural damages were alleviated by EECP. Moreover, Chinese propolis administration inhibited the overexpression of inflammatory factors (IL-1β, IL-6, IL-10) in mouse mammary gland tissue, and increased the transcription level of tight junction protein genes (Occludin, Claudin-1, ZO-1) mRNA, and enhanced the expression of tight junction protein Occludin, ZO-1. 【Conclusion】EECP showed good preventive effect against lipopolysaccharide-induced mastitis in mice, with potent anti-inflammatory effects. It also increased the expressions of tight junction proteins in the mammary gland, which maintained the integrity of the tight junction structure in the mammary gland and protected the blood-milk barrier. Nevertheless, the molecular mechanism of Chinese propolis on the regulation of tight junctions needed to be clarified in the future research.

Key words: Chinese propolis, lipopolysaccharide, mastitis, blood-milk barrier

SONG MeiJie,OU AiQun,XUE XiaoFeng,WU LiMing,SHOU QiYang,WANG Kai. Protective Effects of Chinese Propolis Extract Against Lipopolysaccharide- Induced Acute Mastitis and Mammary Barrier Functions in Mice[J].Scientia Agricultura Sinica, 2021, 54(12): 2675-2688.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2021.12.017

https://www.chinaagrisci.com/EN/Y2021/V54/I12/2675

Figures/Tables 12

Fig. 1

Table 1

Table 2

Table 3

Table 4

Table 5

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

References 40

[1]	GOMES F, HENRIQUES M. Control of bovine mastitis: Old and recent therapeutic approaches. Current Microbiology, 2016,72(4):377-382.DOI: 10.1007/s00284-015-0958-8. doi: 10.1007/s00284-015-0958-8
[2]	PETERSSON-WOLFE C S, LESLIE K E, SWARTZ T H. An update on the effect of clinical mastitis on the welfare of dairy cows and potential therapies. The Veterinary Clinics of North America: Food Animal Practice, 2018,34(3):525-535. DOI: 10.1016/j.cvfa.2018.07.006. doi: 10.1016/j.cvfa.2018.07.006
[3]	KIBEBEW K. Bovine Mastitis: A review of causes and epidemiological point of view. Journal of Biology, Agriculture and Healthcare, 2017,7(2):1-14.
[4]	BHATTARAI D, WORKU T, DAD R, REHMAN Z U, GONG X L, ZHANG S J. Mechanism of pattern recognition receptors (PRRs) and host pathogen interplay in bovine mastitis. Microbial Pathogenesis, 2018,120:64-70. DOI: 10.1016/j.micpath.2018.04.010. doi: 10.1016/j.micpath.2018.04.010
[5]	赵高乾, 王素英, 李广善, 赵庆彬. 奶牛乳房炎主要病原菌感染流行规律研究进展. 中国畜牧兽医, 2019,46(11):3378-3386.
	ZHAO G Q, WANG S Y, LI G S, ZHAO Q B. Research progress on infection epidemic patterns among major dairy cow mastitis pathogenic bacteria. China Animal Husbandry & Veterinary Medicine, 2019,46(11):3378-3386. (in Chinese)
[6]	ZHANG X, WANG Y N, XIAO C, WEI Z K, WANG J J, YANG Z T, FU Y H. Resveratrol inhibits LPS-induced mice mastitis through attenuating the MAPK and NF-κB signaling pathway. Microbial Pathogenesis, 2017,107:462-467. DOI: 10.1016/j.micpath.2017. 04.002. doi: 10.1016/j.micpath.2017.04.002
[7]	XU T, DONG Z J, WANG X X, QI S P, LI X R, CHENG R, LIU X, ZHANG Y, GAO M Q. IL‐1β induces increased tight junction permeability in bovine mammary epithelial cells via the IL-1β- ERK1/2-MLCK axis upon blood‐milk barrier damage. Journal of Cellular Biochemistry, 2018,119(11):9028-9041. DOI: 10.1002/jcb. 27160. doi: 10.1002/jcb.v119.11
[8]	TOMAZI T, DOS SANTOS M V. Antimicrobial use for treatment of clinical mastitis in dairy herds from Brazil and its association with herd-level descriptors. Preventive Veterinary Medicine, 2020,176:104937. DOI: 10.1016/j.prevetmed.2020.104937. doi: 10.1016/j.prevetmed.2020.104937
[9]	PASUPULETI V R, SAMMUGAM L, RAMESH N, GAN S H. PROPOLIS H, JELLY R. A Comprehensive review of their biological actions and health benefits. Oxidative Medicine and Cellular Longevity, 2017: 1259510. DOI: 10.1155/2017/1259510.
[10]	梁泽宇, 余秋恩, 尹佳隆, 孙圣伟, 何健, 李坤平. 巴西蜂胶和国产蜂胶总黄酮、总酚酸含量及自由基清除活性的研究. 广东药科大学学报, 2019,35(4):493-497, 505.
	LIANG Z Y, YU Q E, YIN J L, SUN S W, HE J, LI K P. Study on the content of total flavonoids and total phenolic acids in Brazilian propolis and Chinese propolis and their free radical scavenging capacity. Journal of Guangdong Pharmaceutical University, 2019,35(4):493-497, 505. (in Chinese)
[11]	WANG K, JIN X L, SHEN X G, SUN L P, WU L M, WEI J Q, MARCUCCI M C, HU F L, LIU J X, KO S G. Effects of Chinese propolis in protecting bovine mammary epithelial cells against mastitis pathogens-induced cell damage. Mediators of Inflammation, 2016: 8028291. DOI: 10.1155/2016/8028291.
[12]	BACIC G, MACESIC N, RADIN L, ALADROVICC J, MATANOVIC K, MASEK T, BROZIC D, Benić M, RADIC B, BACIC I, ŠURAN J. Intramammary propolis formulation for prevention and treatment of mastitis in dairy ruminants (RC.2.2.08- 0003). Journal of Animal Research, 2016,6(2):227-229. DOI: 10. 5958/2277-940X.2015.00165.5. doi: 10.5958/2277-940X.2015.00165.5
[13]	BURMANCZUK A, HOLA P, MILCZAK A, PIECH T, KOWALSKI C, WOJCIECHOWSKA B, GRABOWSKI T. Quercetin decrease somatic cells count in mastitis of dairy cows. Research in Veterinary Science, 2018,17:225-229. DOI: 10.1016/j.rvsc.2018.01.006.
[14]	翟晓虎, 徐传芬, 张玲玲, 贺卫华, 马霞, 杨俊花. 蜂胶黄酮作为PPV灭活疫苗佐剂活性的研究. 上海农业学报, 2019,35(1):71-75.
	ZHAI X H, XU C F, ZHANG L L, HE W H, MA X, YANG J H. Adjuvant activity of propolis flavone for PPV vaccine. Acta Agriculturae Shanghai, 2019,35(1):71-75. (in Chinese)
[15]	STELWAGEN K, SINGH K. The role of tight junctions in mammary gland function. Journal of Mammary Gland Biology and Neoplasia, 2014,19(1):131-138. DOI: 10.1007/s10911-013-9309-1. doi: 10.1007/s10911-013-9309-1
[16]	PARK H Y, KUNITAKE Y, HIRASAKI N, TANAKA M, MATSUI T. Theaflavins enhance intestinal barrier of Caco-2 Cell monolayers through the expression of AMP-activated protein kinase-mediated Occludin, Claudin-1, and ZO-1. Bioscience, Biotechnology, and Biochemistry, 2015,79(1):130-137. doi: 10.1080/09168451.2014.951027
[17]	欧爱群, 王凯, 吴黎明, 李江红, 彭文君. 蜂胶对细菌脂多糖刺激下奶牛乳腺上皮细胞炎症相关基因mRNA转录水平和紧密连接蛋白的影响. 畜牧兽医学报, 2020,51(5):1141-1148.
	OU A Q, WANG K, WU L M, LI J H, PENG W J. Effects of propolis on transcript levels of inflammation-related genes and tight junction proteins of bovine mammary epithelial cells stimulated by bacterial lipopolysaccharide. Chinese Journal of Animal and Veterinary Sciences, 2020,51(5):1141-1148. (in Chinese)
[18]	TRUSHEVA B, TRUNKOVA D, BANKOVA V. Different extraction methods of biologically active components from propolis: a preliminary study. Chemistry Central Journal, 2007,1(1):1-4. DOI: 10. 1186/1752-153X-1-13. doi: 10.1186/1752-153X-1-1
[19]	SFORCIN J M. Biological properties and therapeutic applications of propolis. Phytotherapy Research, 2016,30(6):894-905. DOI: 10.1002/ptr.5605. doi: 10.1002/ptr.5605
[20]	DEVEQUI-NUNES D, MACHADO B A S, BARRETO G D A, REBOUÇAS SILVA J, DA SILVA D F, DA ROCHA J L C, BRANDÃO H N, BORGES V M, UMSZA-GUEZ M A. Chemical characterization and biological activity of six different extracts of propolis through conventional methods and supercritical extraction, PLoS ONE, 2018,13(12):e207676.
[21]	BREYNE K, De VLIEGHER S, De VISSCHER A, PIEPERS S, MEYER E. Technical note: A pilot study using a mouse mastitis model to study differences between bovine associated coagulase- negative staphylococci. Journal of Dairy Science, 2015,98(2):1090-1100. DOI: 10.3168/jds.2014-8699. doi: 10.3168/jds.2014-8699
[22]	CAMPERIO C, ARMAS F, BIASIBETTI E, FRASSANITO P, GIOVANNELLI C, SPURIA L, D AGOSTINO C, TAIT S, CAPUCCHIO M T, MARIANELLI C. A mouse mastitis model to study the effects of the intramammary infusion of a food-grade Lactococcus lactis strain. PLoS ONE, 2017,12(9):e0184218. DOI: 10.1371/journal.pone.0184218. doi: 10.1371/journal.pone.0184218
[23]	WANG K, JIN X L, SHEN X G, CHEN Y F, SONG Z H, JIANG X S, HU F L, CONLON M A, TOPPING D L. Polyphenol-rich propolis extracts strengthen intestinal barrier function by activating AMPK and ERK signaling. Nutrients, 2016,8(5):272-283. DOI: 10.3390/ nu8050272. doi: 10.3390/nu8050272
[24]	WANG K, ZHANG J L, PING S, MA Q X, CHEN X, XUAN H Z, SHI J H, ZHANG C P, HU F L. Anti-inflammatory effects of ethanol extracts of Chinese propolis and buds from poplar (Populus× canadensis). Journal of Ethnopharmacology, 2014,155(1):300-311. doi: 10.1016/j.jep.2014.05.037
[25]	王蓓, 常化松, 苏松坤, 孙丽萍, 王凯. 无刺蜂蜂胶乙醇提取物的体外抗氧化及抗炎活性. 中国农业科学, 2019,52(05):939-948.
	WANG B, CHANG H S, SU S K, SUN L P, WANG K. Antioxidative and anti-inflammatory activities of ethanol extract of geopropolis from stingless bees. Scientia Agricultura Sinica, 2019,52(05):939-948. (in Chinese)
[26]	WANG K, HU L, JIN X L, MA Q X, MARCUCCI M C, NETTO A A L, SAWAYA A C H F, HUANG S, REN W K, CONLON M A, TOPPING D L, HU F L. Polyphenol-rich propolis extracts from China and Brazil exert anti-inflammatory effects by modulating ubiquitination of TRAF6 during the activation of NF-κB. Journal of Functional Foods, 2015,19:464-478. DOI: 10.1016/j.jff.2015.09. 009. doi: 10.1016/j.jff.2015.09.009
[27]	WANG K, JIN X L, LI Q Q, SAWAYA A C H F, LE LEU R K, CONLON M A, WU L M, HU F L. Propolis from different geographic origins decreases intestinal inﬂammation and bacteroides spp. populations in a model of DSS-induced colitis. Molecular Nutrition & Food Research, 2018,62(17):e1800080. DOI: 10.1002/mnfr.201800080.
[28]	PATEL S. Emerging adjuvant therapy for cancer: Propolis and its constituents. Journal of Dietary Supplements, 2016,13(3):245-268. DOI: 10.3109/19390211.2015.1008614. doi: 10.3109/19390211.2015.1008614
[29]	陈磊, 刘芸, 陈雷, 张晓燕, 冯峰, 张峰. 高效液相色谱-四极杆飞行时间质谱检测中国杨树型蜂胶、巴西绿蜂胶和杨树胶中的酚类化合物及真伪鉴别. 色谱, 2019,37(1):40-45.
	CHEN L, LIU Y, CHEN L, ZHANG X Y, FENG F, ZHANG F. Determination of phenolic compounds in Chinese poplar propolis,Brazil green propolis,and poplar gum by high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry and preliminary study of the identification of adulteration. Chinese Journal of Chromatography, 2019,37(1):40-45. (in Chinese)
[30]	楚爱景, 程旭锋, 赵慧朵, 王伟. 蒲公英甾醇对脂多糖诱导乳腺炎大鼠的抗炎作用及其机制. 东南大学学报(医学版), 2019,38(2):303-308.
	CHU A J, CHENG X F, ZHAO H D, WANG W. Anti-inflammatory effects and mechanisms of taraxasterol on lipopolysaccharide-induced mastitis in rats. Journal of Southeast Univ (Medical Sci Ed), 2019,38(2):303-308. (in Chinese)
[31]	LIU M J, SONG S X, LI H R, JIANG X Y, YIN P, WAN C R, LIU X X, LIU F H, XU J Q. The protective effect of caffeic acid against inflammation injury of primary bovine mammary epithelial cells induced by lipopolysaccharide. Journal of Dairy Science, 2014,97(5):2856-2865. DOI: 10.3168/jds.2013-7600. doi: 10.3168/jds.2013-7600
[32]	BUENO-SILVA B, FRANCHIN M, De FREITAS ALVES C, DENNY C, COLÓN D F, CUNHA T M, ALENCAR S M, NAPIMOGA M H, ROSALEN P L. Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process. Phytomedicine, 2016,23(13):1583-1590. DOI: 10.1016/j.phymed.2016.09.009. doi: 10.1016/j.phymed.2016.09.009
[33]	FURUSE M, HIRASE T, ITOH M, NAGAFUCHI A, YONEMURA S, TSUKITA S, TSUKITA S. Occludin: a novel integral membrane protein localizing at tight junctions. The Journal of Cell Biology, 1993,123(6):1777-1788. doi: 10.1083/jcb.123.6.1777
[34]	MCCARTHY K M, SKARE I B, STANKEWICH M C, FURUSE M, TSUKITA S, ROGERS R A, LYNCH R D, SCHNEEBERGER E E. Occludin is a functional component of the tight junction. Journal of Cell Science, 1996,109(9):2287-2298. doi: 10.1242/jcs.109.9.2287
[35]	SAITOU M, FURUSE M, SASAKI H, SCHULZKE J D, FROMM M, TAKANO H, NODA T, TSUKITA S. Complex phenotype of mice lacking occludin, a component of tight junction strands. Molecular Biology of the Cell, 2000,11(12):4131-4142. doi: 10.1091/mbc.11.12.4131
[36]	徐姝燕, 富建华. 紧密连接相关蛋白Occludin的研究进展. 国际儿科学杂志, 2012,39(5):451-454.
	XU S Y, FU J H. Progress of tight junction associated protein occludin. International Journal of Pediatrics, 2012,39(5):451-454. (in Chinese)
[37]	FANNING A S, MA T Y, ANDERSON J M. Isolation and functional characterization of the actin binding region in the tight junction protein ZO-1. The FASEB Journal, 2002,16(13):1-23. DOI: 10.1096/fj.02-0121fje.
[38]	陈思远, 刘雪, 罗文新. 紧密连接蛋白claudins应用于肿瘤治疗的进展. 生物工程学报, 2019,35(6):931-941.
	CHEN S Y, LIU X, LUO W X. Advances in the application of claudins to tumor therapy. Chinese Journal of Biotechnology, 2019,35(6):931-941. (in Chinese)
[39]	JIANG A M, ZHANG Y, ZHANG X, WU D, LIU Z Y, LI S Q, LIU X, HAN Z, WANG C Q, WANG J J, WEI Z K, GUO C M, YANG Z T. Morin alleviates LPS-induced mastitis by inhibiting the PI3K/AKT, MAPK, NF-κB and NLRP3 signaling pathway and protecting the integrity of bloodmilk barrier. International Immunopharmacology, 2020,78:1-9. https://doi.org/10.1016/j.intimp. 2019.105972.
[40]	AL-WAILI N. Mixing two different propolis samples potentiates their antimicrobial activity and wound healing property:A novel approach in wound healing and infection. Veterinary World, 2018,11:1188-1195. doi: 10.14202/vetworld.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

试剂 Re agent	体积 Volume (μL)
PrimeScript RT Enzyme Mix	2
Total RNA	2
RNase Free DH₂O	加至10 Add up to 10

基因 Genes	引物序列 Primer sequence （5′-3′）	产物长度 Product length (bp)
Occludin	F: TCTGCTTCATCGCTTCCTTAG R: GTCGGGTTCACTCCCATTA	160
ZO-1	F: ACTCCCACTTCCCCAAAAAC R: CCACAGCTGAAGGACTCACA	166
Cluadin-1	F: AGACCTGGATTTGCATCTTGGTG R: TGCAACATAGGCAGGACAAGAGTTA	126
GAPDH	F: GAGAAACCTGCCAAGTATGATGAC R: TAGCCGTATTCATTGTCATACCAG	212

成分 Components	体积 Volume (μL)
TB green	5
PCR上游引物 Forward primers	0.2
PCR下游引物 Reverse primers	0.2
cDNA	0.2
ddH₂O	4.4
Total	10

多酚类化合物 Polyphenolic compound	平均回收率（RSD,n=5） Recovery rate (RSD, n-5) (%)
咖啡酸Caffeic Acid	100.6-105.6（1.35-3.87）
p-香豆酸 p-Coumaric acid	84.6-96.3（0.70-2.10）
阿魏酸 Ferulic Acid	92.7-100.4（1.42-3.90）
咖啡酸苯乙酯 Caffeic acid phenethyl ester	83.2-101.7（0.96-2.89）
芹菜素 Apigenin	86.8-93.4（0.62-3.13）
柯因Chrysin	90.3-97.8（0.93-1.91）
槲皮素 Quercetin	95.9-98.1（1.01-3.15）
高良姜素 Galangin	98.2-117.4（1.23-1.49）
桑色素 Morin	70.2-91.7（2.02-6.30）
松属素 Pinocembrin	104.5-120.1（0.61-1.73）
短叶松素 Pinobanksin	99.1-100.4（0.45-5.11）
芦丁Rutin	78.3-85.7（2.03-4.12）
短叶松素三乙酸酯 Pinobanksin-3-acetate	96.6-111.2（3.13-6.12）
杨梅素 Myricetin	74.6-80.8（2.87-4.40）
香草酸 Vanillic acid	81.5-94.2（0.61-3.16）
柚皮素 Naringenin	105.2-113.0（0.71-2.72）
3,4-二甲氧基肉桂酸 3,4-Dimethoxycinnamic acid	99.4-102.6（0.99-4.67）

多酚类化合物 Polyphenolic compound	分子式 Molecular formula	保留时间 Rt (min)	母离子 Parention	含量 Content (μg·mg^-1)
咖啡酸Caffeic Acid	C9H8O4	5.487	179.035	3.23±0.03
p-香豆酸 p-Coumaric acid	C9H8O3	6.109	163.0401	-
阿魏酸 Ferulic Acid	C10H10O4	6.304	193.0506	-
咖啡酸苯乙酯 Caffeic acid phenethyl ester	C17H16O4	9.503	283.0976	2.49±0.06
芹菜素 Apigenin	C15H10O5	7.718	269.0455	1.68±0.08
柯因Chrysin	C15H10O4	9.363	253.0506	7.71±1.01
槲皮素 Quercetin	C15H10O7	7.225	301.0354	1.51±0.05
高良姜素 Galangin	C15H10O5	9.547	269.0455	12.88±0.57
桑色素 Morin	C15H10O7	6.919	301.0354	-
松属素 Pinocembrin	C15H12O4	9.461	255.0663	8.56±0.27
短叶松素 Pinobanksin	C15H12O5	8.000	271.0612	8.52±0.25
芦丁Rutin	C27H30O16	5.599	609.1461	-
短叶松素三乙酸酯 Pinobanksin-3-acetate	C17H14O6	9.609	313.0718	12.93±0.59
杨梅素 Myricetin	C15H10O8	6.580	317.0303	0.81±0.06
香草酸 Vanillic acid	C8H8O4	5.597	167.035	0.72±0.01
柚皮素 Naringenin	C15H12O5	7.767	271.0612	-
3,4-二甲氧基肉桂酸 3,4-Dimethoxycinnamic acid	C11H12O4	7.120	207.0663	3.49±1.23

Protective Effects of Chinese Propolis Extract Against Lipopolysaccharide- Induced Acute Mastitis and Mammary Barrier Functions in Mice

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 12

References 40

Related Articles 15

Metrics

Comments

Recommended 0

[1]	XIA YuXin,LIANG Yan,WANG HaiYang,GUO MengLing,ZHOU Bu,DAI Xu,YANG ZhangPing,MAO YongJiang. Effects of the Number of Subclinical Mastitis and Somatic Cell Score in Milk of Parity 1 on Somatic Cell Score of Holstein Cows for Parity 2 [J]. Scientia Agricultura Sinica, 2022, 55(20): 4052-4064.
[2]	CHEN Zhi,ZHANG Yi,LU QinYue,GUO JiaHe,LIANG Yan,ZHANG MingYiXing,YANG ZhangPing. Effect and Mechanism of Tea Tree Oil on LPS Induced Mastitis in Dairy Cows [J]. Scientia Agricultura Sinica, 2021, 54(14): 3124-3133.
[3]	LI GuangDong,LÜ DongYing,TIAN XiuZhi,JI PengYun,GUO JiangPeng,LU YongQiang,LIU GuoShi. Research Progress of Omics Technologies in Cow Mastitis [J]. Scientia Agricultura Sinica, 2019, 52(2): 350-358.
[4]	YING ShiJia, DAI ZiChun, GUO JiaJia, SHI ZhenDan. Time Course Effect of Lipopolysaccharide on Toll-Like Receptors Expression in the Goose Follicular Stroma [J]. Scientia Agricultura Sinica, 2017, 50(6): 1147-1156.
[5]	WANG Xiao, ZHANG Qin, YU Ying. Genome-Wide Association Study on Mastitis Resistance Based on Somatic Cell Scores in Chinese Holstein Cows [J]. Scientia Agricultura Sinica, 2017, 50(4): 755-763.
[6]	WANG MengQi, NI Wei, ZHANG HuiMin, YANG ZhangPing, WANG XiPu, JIANG YanSen, MAO YongJiang. Association Between SNPs in the CDS Regions of CXCR1 Gene and the Clinical Mastitis and Lifetime for Chinese Holstein [J]. Scientia Agricultura Sinica, 2017, 50(12): 2359-2370.
[7]	WANG Lin-feng, JIA Shao-dan, YANG Gai-qing, ZHU He-shui, LIU Ru-yi, YAN Ping, LI Ming, YANG Guo-yu. Study on the Effect of Lipopolysaccharide on Hepatic Metabolism in Dairy Goat Liver [J]. Scientia Agricultura Sinica, 2015, 48(18): 3701-3710.
[8]	YING Shi-jia, XU Ai-hong, DAI Zi-chun, XI Zheng-lin, HE Zong-liang, KUANG Wei, ZHAO Wei, WU Yun-liang, SHI Zhen-dan . Effects of Pro-Biotic Microbes on Living Water and Laying Performance in Layer Ducks and Molecular Mechanisms [J]. Scientia Agricultura Sinica, 2015, 48(11): 2262-2269.
[9]	SUN Yao-gui, CHENG Jia, LI Hong-quan, WANG Jun-dong. Effects of CZKJKL on Stress-Related Factors of Nerve - Endocrine - Immune System of Rats After Immunological Induction [J]. Scientia Agricultura Sinica, 2014, 47(23): 4718-4725.
[10]	XU Ji-Ying, YANG Zhi-Qiang, CHEN Hua-Qi, LIU Jun-Lin, XING Juan, LI Jian-Xi, LI Hong-Sheng. Detection of High Pathogenicity Islands in Escherichia coli Isolated from Clinical and Subclinical Bovine Mastitis in Some Areas of China [J]. Scientia Agricultura Sinica, 2012, 45(6): 1199-1205.
[11]	SU Yang-., PU Wan-Xia, CHEN Zhi-Hua, DENG Hai-Ping. Antimicrobial Resistance Analysis and Detection of Methicillin- Resistant Staphylococcus aureus (MRSA) Among Staphylococcus aureus Strains Isolated from Bovine Mastitis [J]. Scientia Agricultura Sinica, 2012, 45(17): 3602-3607.
[12]	MAO Yong-jiang, CHEN Ren-jin, CHEN Ying, CHANG Ling-ling, SHI Xue-kui,ZHANG Ya-qin, YANG Zhang-ping. Using the MDR Model to Analyze the Relationship of Gene-Gene Interaction Between CXCR1 and IL-8 and the Risk of Mastitis Susceptibility of Chinese Holstein [J]. Scientia Agricultura Sinica, 2011, 44(9): 1908-1915.
[13]	FU Da-bo,WANG You-wei,HOU Yong-qing,DING Bin-ying,WANG Lei,LIU Yu-lan,ZHU Hui-ling . Effects of α-Ketoglutarate on Muscle Energy Metabolism in Weanling Pigs Chronically Challenged with Lipopolysaccharide [J]. Scientia Agricultura Sinica, 2011, 44(4): 814-822 .
[14]	NI Chun-xia,PU Wan-xia,HU Yong-hao,DENG Hai-ping . Research on Coagulase Genotyping of Staphylococcus Aureus Isolated from Bovine Mastitis [J]. Scientia Agricultura Sinica, 2011, 44(2): 417-422 .
[15]	GUO Yang, WANG Hong-Mei, HOU Ming-Hai, WANG Chang-Fa, HE Hong-Bin, LU Wen-Fa , ZHONG Ji-Feng. Polymorphisms of SLC11A1 Gene and Their Association with Mastitis in Chinese Holstein [J]. Scientia Agricultura Sinica, 2011, 44(19): 4072-4080.