不同苦瓜品种皂苷含量组成及其抗氧化活性和α-葡萄糖苷酶的抑制活性

doi:10.3864/j.issn.0578-1752.2017.17.015

Abstract

Abstract: 【Objective】 The content and composition of saponion of 13 different M. charantia varieties as well as the antioxidant activity and inhibitory effect of α-glucosidase were studied. 【Method】 Perchloric acid-vanilin-glacial method was used to determine saponin content, while HPLC method was used to measure the contents of 7 saponin components. In addition, their antioxidant activity was evaluated by oxygen radical absorbance capacity (ORAC). The 4-nitrophenyl-2-β-D-glucopyranoside method was used to measure the α-glucosidase inhibitory activity and to analyze the relationship between the components and the corresponding activity. 【Result】There is a significant difference in contents of saponin in 13 different varieties of M. charantia.The content ranges of saponin were 0.52-1.20 g/100g DW, with an average value being 0.79 g/100g DW, and the coefficient of variability being 21.65%. The average contents of components were as follows: momorcharaside A 5.32 μg·g^-1DW, momordicoside A 25.42 μg·g^-1 DW, karaviloside XI 3.96 μg·g^-1DW, momordicoside F2 66.95 μg·g^-1DW, momordicoside K 183.70 μg·g^-1DW, (23E)-3β,7β,25-trihydroxycucubita-5,23-dien-19-al 40.13 μg·g^-1 DW, and kuguacin N 3.87 μg·g^-1 DW. ORAC values of the 13 M. charantia varieties varied from 2 747.76 to 15 584.07 μmol Trolox?g^-1, the average value being 8 879.48 mol Trolox·g^-1, the coefficient of variability being 34.91% and the IC₅₀ value of alpha glucosidase varied from 1.55 to 4.96 mg·mL^-1. 【Conclusion】Significant differences in the components of saponin and the antioxidant activity and the inhibitory effect on α-glucosidase of different varieties of M. charantia were detected. Saponin is the main active basis of α-glucosidase activity in M. charantia, but not the main antioxidant substance. (23E)-3β,7β,25-trihydroxycucubita-5,23-dien-19-al is the major active compound.

Key words: Momordica charantia, saponin, antioxidant, α-glucosidase

LIU HuiJuan, ZHANG MingWei, ZHANG RuiFen, ZHANG Yan, WEI ZhenCheng, MA YongXuan, LIU Lei, DENG YuanYuan. Saponion Profiles and Antioxidant Activity, α-Glucosidase Inhibitory Activity of Momordica charantia of Different Varieties[J].Scientia Agricultura Sinica, 2017, 50(17): 3413-3421.

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Saponion Profiles and Antioxidant Activity, α-Glucosidase Inhibitory Activity of Momordica charantia of Different Varieties

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