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    01 June 2023, Volume 56 Issue 11
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    Analysis and Comprehensive Evaluation of Phenotype Genetic Diversity in Kam Sweet Rice Germplasm Resources in Guizhou
    LI Huan, YAN XiaoQing, YANG ZhanLie, TAN JinYu, LI XiaoBing, CHEN NengGang, WU RongJu, CHEN HuiCha, RUAN RenChao
    Scientia Agricultura Sinica. 2023, 56(11):  2035-2046.  doi:10.3864/j.issn.0578-1752.2023.11.001
    Abstract ( 418 )   HTML ( 64 )   PDF (1446KB) ( 977 )   Save
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    【Objective】To analyze the phenotypic genetic diversity of traditional characteristic landraces of Kam Sweet Rice (KSR) in Guizhou, this study screened the comprehensive evaluation indicators for phenotype, and constructed a reliable mathematical model for comprehensive evaluation on phenotypes. This study provides valuable theoretical support for the discovery and breeding of exceptional KSR germplasm resources. 【Method】13 phenotypic traits from a total of 286 KSR accessions collected from the Southeast Guizhou were measured. A variety of multiple statistical methods, including Shannon-Wiener genetic diversity index, principal component analysis, subordinate function value analysis, and stepwise regression analysis, were used to analyze the phenotypic genetic diversity and comprehensively evaluate on KSR germplasm resources. 【Result】Firstly, the KSR germplasm showed high phenotypic genetic diversity, with the variation coefficients of the 13 phenotypic traits ranging from 6.79% (Grain width) to 30.73% (Panicle number per plant), and the diversity index (H') ranging from 2.484 (Ratio of length to width for grain) to 2.996 (Flag leaf width). Correlation analysis showed significant or highly significant correlations among the different traits. Principal component analysis showed that the 13 traits were integrated into 7 principal components, with contribution rates ranging from 8.44% to 23.14%, and the additive contributing rate came up to 90.29%. The phenotypic comprehensive evaluation D value calculated by subordinate function values analysis showed that the top 5 varieties had the best characteristics, and 11 phenotypic traits were significantly correlated with the D value. The stepwise regression analysis established a mathematical model for phenotypic evaluation of KSR, Y=-0.249+0.119X5+0.395X13+0.071X6-0.161X3+0.108X10+0.170X2+0.110X9 (F=2800.200, R2=0.986). Based on the model, 7 comprehensive evaluation indicators were screened out. At last, the 286 germplasm resources were systematically clustered into four categories based on the D value, displaying significant differences among the groups and outstanding characteristics. The group I, including 38 accessions, showed the best comprehensive traits and high yield potential; the group Ⅱ, including 103 accessions, showed general comprehensive traits and high seed setting rate; the group Ⅲ, including 94 accessions, showed poor comprehensive traits and long growth period; the group Ⅳ, including 51 accessions, had the worst comprehensive traits. 【Conclusion】The KSR germplasm resources in Guizhou have abundant phenotypic genetic diversity. It is feasible to use multiple statistical analysis methods for comprehensive evaluation on KSR germplasm diversity. The regression equation constructed under the same conditions can quantitatively evaluate the comprehensive performance of KSR germplasm resources. The filled grains per panicle, grain width, seed setting rate, panicle number per plant, flag leaf length, plant height and grain yield per plant can be used for identifying KSR germplasm resources. The outstanding germplasm resources with coordinated comprehensive traits such as Zaohe, Nuohe-12, 90 Tianhe, Goudong-1 and Nuohe-11 were screened out, which can be ultilized for genetic improvement of KSR and for rice breeding.

    Genome-Wide Association Study of Grain Main Quality Related Traits in Winter Wheat
    DONG YiFan, REN Yi, CHENG YuKun, WANG Rui, ZHANG ZhiHui, SHI XiaoLei, GENG HongWei
    Scientia Agricultura Sinica. 2023, 56(11):  2047-2063.  doi:10.3864/j.issn.0578-1752.2023.11.002
    Abstract ( 414 )   HTML ( 38 )   PDF (3371KB) ( 2581 )   Save
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    【Objective】The quality of wheat grain was an important factor affecting the processing quality and nutritional. Mining loci and candidate genes significantly associated with wheat grain quality traits provided a basis for broadening the understanding of the genetic mechanism of quality traits and molecular marker-assisted quality. 【Method】By measuring five quality traits, including protein content (GPC), wet gluten content (WGC), starch content (GSC), settling value (SV) and grain hardness (GH), in 259 winter wheat varieties (lines) from domestic and abroad, and conducting genome-wide association analysis in combination with 90K SNP chip, the significant association loci located were subjected to haplotype analysis. 【Result】All five traits conformed to normal distribution and showed rich variation among different environments, and the coefficient of variation of sedimentation value was the largest (20.11%-24.42%). All traits have shown highly significant differences (P<0.001) among genotype, environment, and genotype×environment, with a broad-sense heritability of 0.77-0.84. A total of 44 loci significantly associated (P<0.001) with five traits were detected by genome-wide association analysis, distributed in 19 linkage groups other than chromosomes 1D and 3D. Eighteen loci were stable in two or more environments, involving all five traits including protein content (12), wet gluten content (9), starch content (11), sedimentation value (12) and grain hardness (7), explaining 4.27%-10.98% of the genetic variation. Thirteen of them were multi-effect loci, with the largest number of multi-effect loci (7) associated with traits such as protein content, wet gluten content, settling value and starch content. The GENE-0762_631, IAAV7742 and RAC875_c66845_466 loci located on 2B, 2D and 3A chromosomes were detected simultaneously at two environmental and BLUP values with a range of 4.32%-7.07% phenotypic contribution. Through haplotype analysis of multi-effect loci present in multiple environments with high phenotypic contribution, four different haplotypes, Hap1, Hap2, Hap3 and Hap4, which were significantly associated with traits such as protein content, sedimentation value and starch content, were uncovered at the D_GDS7LZN02F4FP5_176 locus of chromosome 5D, among them Hap1 was a high starch content haplotype (P<0.001), while Hap2 and Hap3 were both haplotypes with high protein content and sedimentation value (P<0.05), and the four haplotypes accounted for 74.22%, 16.21%, 6.92% and 2.65%, respectively. The distribution frequencies of haplotypes from different sources of winter wheat were analyzed, in which the distribution frequencies of haplotype Hap2 with high protein content and sedimentation value were from high to low in the Huanghuai winter wheat regions>northern winter wheat region>abroad varieties>middle and lower reaches of the Yangtze River winter wheat region>southwest winter wheat region. Candidate genes were mined for stable genetic loci, and 10 candidate genes that might be related to wheat grain quality were screened. 【Conclusion】In the study, 18 stable loci significantly associated with grain quality traits were detected, 4 different haplotypes were identified, and 10 candidate genes related to grain quality were screened.

    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY
    Analysis of Common Characteristics of Widely Adaptation Wheat Cultivars
    LÜ LiHua, HAN JiangWei, ZHANG JingTing, DONG ZhiQiang, MENG Jian, JIA XiuLing
    Scientia Agricultura Sinica. 2023, 56(11):  2064-2077.  doi:10.3864/j.issn.0578-1752.2023.11.003
    Abstract ( 322 )   HTML ( 26 )   PDF (614KB) ( 1883 )   Save
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    【Objective】 The objective of this study was to provide simple detection indicators for the screening of stress resistant and widely adapted cultivars under the background of frequent meteorological disasters in the North HuangHuaiHai Plain, through to screen drought resistant, heat-resistant and cold resistant winter wheat cultivars, and to clarify the yield composition, plant type structure and physiological characteristics of stress resistant and widely suitable cultivars.【Method】A field and a greenhouse experiments (experiment 1 and experiment 2, respectively) were carried out in Gaocheng of Hebei province from Autumn 2017 to Summer 2020, and using 16 winter wheat cultivars as experimental material. For experiment 1, three treatments were set up, i.e., 0 irrigation, 1 irrigation (jointing) and 2 irrigation (jointing and flowering). For experiment 2, two treatments were set up at the late stage of filling, i.e., normal temperature treatment (CK) and warming temperature treatment. At the same time, the cold resistance test was conducted using the natural low temperature in the spring of 2018 and 2020. The stress resistance evaluation index, yield formation index, plant type structure index and leaf physiological index were determined.【Result】Five winter wheat cultivars were screened out by comprehensively considering yield, drought resistance index, yield heat sensitivity index and grade of freeze injury, and these cultivars included Jimai 23, Shannong 30, Jimai 325, Jimai 22 and Pinyu 8012. These cultivars had strong drought, heat and cold resistance, and had high and stable yield. By analyzing the correlation between yield and yield formation index, plant type index and leaf physiological index, it was found that 1000-grain weight, harvest index and biomass production were positively correlated with yield; the width of flag leaf, stem diameter and spike length were significantly or extremely significantly positively correlated with yield, while angle of stem and flag leaf was significantly negatively correlated with yield; relative chlorophyll value (SPAD value) and relative water content of flag leaf were positively correlated with yield, and the canopy temperature was negatively correlated with yield. Compared with other cultivars, the 1000-grain weight, harvest index and biomass production of the stress resistant cultivars increased by 12.9%, 5.2% and 3.4%, respectively. For stress resistant wheat cultivars, the width of flag leaf, angle of stem and flag leaf, diameter of basal stem, ear length and plant height were (16.2 ± 0.4) mm, (18.2 ± 3.2)°, (4.0 ± 0.3) mm, (7.5 ± 0.14) cm and (80.3 ± 1.3) cm, respectively. Compared with other cultivars, the SPAD value and relative water content in flag leaves of these five cultivars increased by 9.8% and 4.2% respectively, and the canopy temperature decreased by 1.9 ℃ at the late stage of filling.【Conclusion】The optimized plant type of the stress resistant wheat cultivars, namely “compact in the upper part and flat in the lower part”, was defined. The quantitative indexes were put forward, including width of flag leaf, angle of stem and flag leaf, diameter of basal stem and ear length; the physiological characteristics were put forward, including higher leaf SPAD value and relative water content of flag, and lower canopy temperature; the yield characteristics were identified, including higher 1000-grain weight, harvest index and biomass production.

    Effects of Ridge and Furrow Planting Patterns on Crop Productivity and Soil Nitrate-N Accumulation in Dryland Summer Maize and Winter Wheat Rotation System
    WU JinZhi, HUANG XiuLi, HOU YuanQuan, TIAN WenZhong, LI JunHong, ZHANG Jie, LI Fang, LÜ JunJie, YAO YuQing, FU GuoZhan, HUANG Ming, LI YouJun
    Scientia Agricultura Sinica. 2023, 56(11):  2078-2091.  doi:10.3864/j.issn.0578-1752.2023.11.004
    Abstract ( 289 )   HTML ( 23 )   PDF (1602KB) ( 504 )   Save
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    【Objective】The aim of the present study was to discuss the effects of different ridge and furrow planting patterns on crop productivity, soil properties and soil nitrate-N accumulation, and thus provided a scientific basis for improving soil fertility, increasing crop yield and water use efficiency, and alleviating environmental risks in summer maize-winter wheat rotation system (namely maize-wheat) in dryland.【Method】A study was carried out at the Luoyang Dry Farming Experimental of the Chinese Academy of Agricultural Sciences based on the long-term field experiment initiated in 2004. The experiment included five treatments: permanent ridge and furrow and 6 row wheat planted in ridge (6RPRF), ridge and furrow in each year and other managements kept with 6RPRE (6REYRF), permanent ridge and furrow and 4 row wheat planted in ridge (4RPRF), ridge and furrow in each year and other managements kept with 4RPRF (4REYRF), and conventional flat planting pattern according to the local farmer (CF). The effects of different treatments on the grain yield, water use efficiency of summer maize, winter wheat and the annual in 2015-2021, and the bulk density, nutrient content and enzyme activity in the 0-40 cm soil layer at harvest of summer maize in 2020, and the nitrate-N accumulation in the 0-380 cm soil profile at harvest of winter wheat in 2019-2020 was investigated. 【Result】 Compared with CF, the four ridge and furrow planting patterns improved grain yield in summer maize, winter wheat, and all year by 8.6%-32.1%, 12.5%-25.6%, and 11.3%-29.6%, respectively, and water use efficiency by 8.6%-31.4%, 12.5%-31.1% and 12.8%-30.3%, respectively, averaged across the 6 experimental years from 2015 to 2021. They also significantly decreased the soil bulk density by 7.3%-11.3% in 0-5 cm soil layer and by 4.9%-11.5% in 20-40 cm soil layer, respectively, increased the average content of organic matter by 6.0%-19.8%, total nitrogen by 80.8%-100.0%, available phosphors by 28.5%-80.9%, available potassium by 58.5%-141.2%, urease activity by 24.0%-46.9% in 0-40 cm soil layer, as well as increasing the nitrate-N accumulation by 38.8%-116.0% in 0-100 cm soil layer. Among the four ridge and furrow treatments, 4RPRF had the best productivity and the function of improving root layer but decreasing the sub-layer nitrate-N accumulation, in which the nitrate-N accumulation was significantly increased by 38.7% in the 0-100 cm soil layer but significantly decreased by 15.0% in 200-380 cm soil layer with the total amount in 0-380 cm soil layer maintaining at CF level. Compared with ridge and furrow in each year (EYRF), the 6 year average grain yield in summer maize and all year in permanent ridge and furrow (PRF) treatments were increased by 10.55% and 9.10%, respectively, as well as the WUE in summer maize under 6 rows wheat planted in ridge (6R) pattern, in winter wheat under 4 rows wheat planted in ridge (4R) pattern and in all year were increased by 21.08%, 15.06% and 8.23%, respectively. The soil nutrient content under PRF increased in surface layer but decreased in subsoil layer, and the nitrate nitrate-N accumulation decreased by 4.9%-30.2%, compared with EYRF. Compared with 6R pattern, 4R pattern increased the grain yield in summer maize and in all year by 9.9% and 6.8%, as well as the WUE by 7.4%, 16.5% and 6.7%, respectively, in summer maize under EYRF, in winter wheat under PRF and in all year, averaged across the 6 experimental years. Compared with 6R pattern, 4R pattern had a tendency to improve soil properties, but the effect varied with different indexes, ridge raising patterns and soil layer.【Conclusion】4RPRF could not only reduce soil bulk density and increase soil organic matter, total nitrogen and available potassium contents, but also increase the grain yield and water use efficiency in summer maize, winter wheat and all year under most conditions, and also effectively reduce the nitrate-N accumulation in 200-380 cm soil layer. Therefore, 4RPRF was an alternative planting pattern to achieve high yield, high efficiency and environmentally friendly in dryland summer maize-winter wheat rotation system.

    PLANT PROTECTION
    Effect of Broccoli Residues on Enzyme Activity of Cotton Rhizosphere Soil and Relationships Between Enzyme Activity and Carbon Metabolism Characteristics
    ZHAO WeiSong, GUO QingGang, LI SheZeng, LU XiuYun, GOU JianJun, MA Ping
    Scientia Agricultura Sinica. 2023, 56(11):  2092-2105.  doi:10.3864/j.issn.0578-1752.2023.11.005
    Abstract ( 241 )   HTML ( 23 )   PDF (1160KB) ( 1548 )   Save
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    【Objective】The objective of this study is to research the effect of broccoli residues (BR) on enzyme activity of cotton rhizosphere soil and relationships between enzyme activity and carbon source utilization capacity, and to provide a theoretical basis for revealing the ecological mechanism of BR to prevent Verticillium wilt disease and promote the growth of cotton.【Method】In this study, soil treated with BR and soil treated without BR (CK) were set, and the activities of peroxidase, neutral phosphatase, arylsulfatase, chitinase, urease, β-glucosidase and N-acetyl-β-D-glucosaminidase in the rhizosphere soil of different treatments were determined, respectively. Shannon-Wiener index, Simpson index, Pielou index, McIntosh index, and richness index of different treatments were studied by Biolog-ECO technology. Principal component analysis (PCA) was used to compare the characteristics of microbial metabolic activity in rhizosphere soil under different treatments, and redundancy analysis (RDA) was used to analyze the correlations between soil enzyme activity and the utilization capacity of different types of carbon sources.【Result】Compared with the CK, the plant height, branch and boll numbers treated with BR significantly increased by 12.73%, 16.95%, and 10.36%, respectively. The disease index of cotton Verticillium wilt treated with BR decreased by 64.19%. BR treatment significantly increased pH, nitrate nitrogen (NO3--N) and organic matter (OM) contents in rhizosphere soil. Meanwhile, there was no significant difference in the functional diversity index of rhizosphere microorganisms between treatments. However, BR changed the metabolic activities of rhizosphere microorganisms. BR treatment significantly increased the activity of enzymes related to cycles of different nutrient elements. In the aspect of carbon cycle, the activity of peroxidase and β-glucosidase was 2.70 and 1.95 times that of the control, respectively. In the nitrogen cycle, the activity of urease, chitinase and N-acetyl-β-D-glucosidase was 1.42, 1.59 and 1.52 times that of the control, respectively. In the phosphorus cycle, the activity of neutral phosphatase was 1.33 times that of the control. In the sulfur cycle, the activity of arylsulfatase was 1.22 times that of the control. RDA showed that soil enzyme activities under the treatment of BR were positively correlated with the utilization capacity of L-phenylalanine, L-threonine, glycogen, 2-hydroxybenzoic acid, itaconic acid, and D-malic acid, respectively.【Conclusion】BR changed the carbon metabolic activity of rhizosphere soil microorganisms, significantly increased the activity of soil nutrient cycling-related enzymes, pH, nitrate nitrogen, and organic matter contents in rhizosphere soil. Meanwhile, different degrees of correlations were found between soil enzyme activity and carbon metabolism characteristics.

    Optimization of Solid State Fermentation for Production of Active Substances Against Plant Pathogenic Fungi from Chaetomium globosum
    LIAO HongJuan, JIANG YuMei, YE Xia, ZHANG ZhiBin, MA TongYu, ZHU Du
    Scientia Agricultura Sinica. 2023, 56(11):  2106-2117.  doi:10.3864/j.issn.0578-1752.2023.11.006
    Abstract ( 230 )   HTML ( 10 )   PDF (790KB) ( 2026 )   Save
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    【Objective】The objective of this study is to optimize the medium composition and fermentation conditions of straw solid-state fermentation of Chaetomium globosum, improve the antifungal activity of fermentation crude extract, and to provide references for the development of biopesticides of C. globosum and the green resource utilization of straw.【Method】Firstly, using the inhibition rate of crude extracts against the mycelia growth of 9 plant pathogenic fungi as evaluation index, straw (rice, wheat, maize, rape) and nitrogen source (bean pulp, wheat bran, ammonium chloride) in the medium were screened to determine the optimal composition of fermentation medium. Then the single factor optimization of fermentation conditions was carried out, and the inhibition rate of crude extracts against Phytophthora capsici was taken as the evaluation index to determine the optimal range of each fermentation condition and the degree of influence on the antifungal activity of crude extracts. Based on the results of single factor optimization, orthogonal design was used to optimize the fermentation conditions. Parameters were as follows: fermentation time 18-36 d, fermentation temperature 26-32 ℃, initial water content of medium 70%-85%, mass ratio of straw to wheat bran 4﹕1-1﹕1, straw particle size 20->100 mesh. Finally, the optimal fermentation conditions for producing antifungal active substances by solid state fermentation of straw from C. globosum were obtained and verified.【Result】When the composition of solid fermentation medium was screened, it was found that the antifungal effect of crude extracts obtained from the medium composed of wheat straw and wheat bran was generally better than that of other medium. In the single factor optimization of fermentation conditions, the inoculation amount of fungal solution had no significant effect on the antifungal effect of crude extract, so the subsequent orthogonal optimization was not carried out. In the orthogonal optimization, the effects of fermentation conditions on the antifungal activity of the crude extracts were extremely significant (P<0.001), and the effects were as follows: mass ratio of wheat straw to wheat bran>fermentation time>initial water content of medium>fermentation temperature>particle size of wheat straw. The optimal fermentation conditions obtained by orthogonal optimization were as follows: fermentation time 24 d, fermentation temperature 26 ℃, initial water content of medium 80%, mass ratio of wheat straw to wheat bran 4﹕1, particle size of wheat straw 60-100 mesh. After optimization of fermentation conditions, the inhibitory rates of 1 mg·mL-1 crude extract from the fermentation of C. globosum against Sclerotinia sclerotiorum, P. capsica, Magnaporthe oryzae, Monilinia fructicola, Fusarium oxysporum, Fusarium culmorum, Sarocladium oryzae, Fusarium graminearum and Rhizoctonia solani were 100%, 92.86%, 85.94%, 83.90%, 76.12%, 73.02%, 66.18%, 58.96% and 52.99%, respectively.【Conclusion】After the optimization of fermentation medium composition and fermentation conditions, the crude extracts of C. globosum had high antifungal activity, which could lay a foundation for the subsequent separation and purification of antifungal active substances produced by straw solid fermentation of C. globosum.

    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    Dynamics Characteristic of Straw Decomposition and Nutrient Release Under Different C/N Ratio
    LI Ran, XU MingGang, SUN Nan, WANG JinFeng, WANG Fei, LI JianHua
    Scientia Agricultura Sinica. 2023, 56(11):  2118-2128.  doi:10.3864/j.issn.0578-1752.2023.11.007
    Abstract ( 434 )   HTML ( 26 )   PDF (541KB) ( 1898 )   Save
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    【Objective】The decomposition, nutrient release characteristics and driving factors of straw under different C/N ratio were studied based on coal mining area, so as to provide as theoretical basis for the full utilization of straw resources and reclaimed soil fertility improvement.【Method】Air-dried maize straw and wheat straw were cut into 2 mm pieces and were selected for decomposition experiment of a coal mining reclamation area in Shanxi Province. Two C/N ratio levels of 25 and 10 were designed in maize straw and wheat straw by supplementing urea, taking no nitrogen application that C/N ratio levels was 52 and 74 as the control. All the straws (8 g in organic carbon) were put into a nylon mesh bag (0.38 µm aperture), and horizontally buried into 15 cm deep of soil. On the 12, 23, 55, 218, 281 and 365 days after buried, samples were collected from the bags to analyze the dynamic of the dry matter residue and nutrient (carbon, nitrogen, phosphorus and potassium). 【Result】During the first 55 days, the adjusting the C/N on 25 with the application N was the best way to accelerate the decomposition of maize straw. Meanwhile, the adjusting the C/N on 10 was the best way to accelerate the decomposition of wheat straw. Application N could significantly promote the release of carbon and phosphorus from maize straw during the first 55 days. Application N could significantly accelerate the release of carbon, nitrogen and phosphorus from wheat straw, but had no significant effect on the release of potassium. The thermal equation of straw decomposition and nitrogen and potassium release was better than the temporal equation, and phosphorus release from straw was not suitable for the exponential decay equation. When the accumulated temperature was 4 600 ℃, the average release rates of carbon, nitrogen, phosphorus and potassium from straw were 49.2%, 39.5%, 40.8% and 90.3%, respectively; When the accumulated temperature reached 1 125 ℃, more than 85% the potassium of straw was released. The decomposition of straw was mainly influenced by the temperature, organic carbon, lignin and hemicellulose. 【Conclusion】Application N could accelerate the decomposition of maize straw and the release of carbon and phosphorus in the early stage, which could significantly accelerate the wheat straw decomposition and the release of carbon, nitrogen and phosphorus too. Temperature could better reflect the process of straw decomposition and nitrogen, potassium release than time, the decomposition of straw was mainly regulated by the temperature, organic carbon, lignin and hemicellulose. Therefore, combined with the hydrothermal conditions in the coal mining area, the straw with an appropriate amount of urea should be returned to the field in the right time to improve the content of nitrogen, phosphorus and potassium of the reclaimed soil.

    Optimizing Nitrogen Fertilizer Rate for High-Yield Maize in Black Soil Region Based on Ecological and Social Benefits
    ZHENG ChunYu, SHA ShanYi, ZHU Lin, WANG ShaoJie, FENG GuoZhong, GAO Qiang, WANG Yin
    Scientia Agricultura Sinica. 2023, 56(11):  2129-2140.  doi:10.3864/j.issn.0578-1752.2023.11.008
    Abstract ( 265 )   HTML ( 16 )   PDF (955KB) ( 1061 )   Save
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    【Objective】Optimizing nitrogen (N) fertilizer rate for maize, by considering ecological and social benefits in combination with agricultural and economic benefits, was studied to promote the sustainable and green maize production in black soil region, and further to realize the multiple goals including higher yield, higher resource use efficiency, improved environment and human health.【Method】Two field experiments were conducted at San-ke-shu (SKS) and Quan-yan-gou (QYG) in typical black soil region from 2017 to 2020, to study the effects of different N fertilizer (urea) rates (0, 50, 100, 150, 200, 250, and 300 kg N·hm-2) on maize yield, N uptake and N recovery efficiency (NUE), and further to evaluate the agronomic, economic, ecological and social benefits (N-derived gross caused by N application, private benefits of removed the N fertilizer cost, ecological benefits of removed environmental pollution cost such as active nitrogen loss and greenhouse gas emission, and social benefit of removed human health harm cost caused by environmental pollution, respectively) in different N rates by using life cycle assessment and comprehensive benefits analysis. The agronomically optimal N rate (AOR), privately optimal N rate (POR), ecologically optimal N rate (EOR) and socially optimal N rate (SOR) were calculated to evaluate the integrated benefits and determine the optimal N fertilizer rate for maize in black soil region. 【Result】 Maize grain yields were significantly affected by N rates, which increased continuously with increasing N input and reached the yield plateau under 200 kg N·hm-2 treatment at both two experimental sites. Under this N rate, the average yields were 10.3 and 11.1 t·hm-2 at SKS and QYG across four experimental years, respectively. The N uptake of maize plants also showed increased trends with increasing N rates, and the highest value in 200 kg N·hm-2 treatment at SKS and QYG (151.9, 161.8 kg N·hm-2, respectively). The NUE of maize showed the highest values in 100 kg N·hm-2 treatment at both two experimental sites, the averages were 70.3% and 72.2%, respectively; and then, decreased with increasing N rates. Based on 4-year results, the ecological and social costs caused by N fertilizer input increased exponentially with the increase of N application rate. The N-derived gross, private benefits, ecological benefits and social benefits of maize increased firstly and then decreased with the increase of N application rate. Based on the curve fitting calculation, the AOR, POR, EOR and SOR were estimated as 236, 225, 215 and 211 kg N·hm-2 at SKS, respectively, and which were 245, 235, 225 and 221 kg N·hm-2 at QYG, respectively. Under AOR condition, maize yields of 10.6 and 11.4 t·hm-2, the Nr losses of 44.4 and 46.8 kg N·hm-2 were obtained at SKS and QYG, respectively, while their ecological benefits were 8 786 and 10 271 yuan/hm2, and social benefits were 8 351 and 9 822 yuan/hm2, respectively. Compared with AOR, by reducing N inputs by 8.8% and 7.9% at SKS and QYG, respectively, EOR increased partial factor productivity from applied N by 9.1% and 8.1%, respectively, while reducing Nr losses by 11.7% and 11.0%, respectively. Compared with EOR, SOR further reduced N inputs by 10.6% and 9.6% at SKS and QYG, respectively, thus reduced Nr losses by 14.0% and 13.1%, respectively, while increasing social benefits by 124 and 119 yuan/hm2, respectively. 【Conclusion】Based on the comprehensive consideration with ecological and social benefits, the optimal N fertilizer rate was determined as 210-220 kg N·hm-2 for maize with yield of 10.5-12.0 t·hm-2, it was suggested that the optimal application of ecological or social benefits should be recommended for maize nutrient management in black soil area, which could synergistically achieve the multiple goals for higher yields and NUE, improved ecological environment and human health.

    HORTICULTURE
    Effects of Row Spacing and Irrigation Amount on Canopy Light Interception and Photosynthetic Capacity, Matter Accumulation and Fruit Quality of Tomato
    CHANG JiaYue, MA XiaoLong, WU YanLi, LI JianMing
    Scientia Agricultura Sinica. 2023, 56(11):  2141-2157.  doi:10.3864/j.issn.0578-1752.2023.11.009
    Abstract ( 233 )   HTML ( 20 )   PDF (1035KB) ( 1946 )   Save
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    【Objective】Photosynthetically active radiation and photosynthetic physiological characteristics of leaves within the canopy were heterogeneous. The response to row spacing and irrigation amount of light interception and photosynthetic capacity of leaves in different parts of tomato canopy were explored in this study. The effects of row spacing and irrigation amount on photosynthetic productivity of tomato canopy were studied in detail, and the comprehensive quality of fruit was analyzed, which provided a theoretical basis for the setting of row spacing and irrigation amount in mechanized cultivation of tomato.【Method】Tomato, the test material, was cultivated in a wide and narrow row, with plant spacing of 35 cm. Small row spacing of 40 cm, and three large row spacing levels were set: 70 cm (P1), 120 cm (P2), and 170 cm (P3). Two irrigation levels were set: conventional irrigation (W1) and light deficit irrigation (W2). The experiment was a full factorial experiment with 6 treatments. The leaf area and light interception amount of each leaf position were measured. The canopy was divided into six parts, and the net photosynthetic rate (Pn), leaf mass per area (LMA), chlorophyll (Chl) and N, P, K content were measured. The canopy photosynthetic capacity under each treatment was comprehensively analyzed by taking the proportion of leaf area of each part to that of the whole plant or the proportion of leaf dry weight of each part to that of the whole plant as weights. The correlation of each index was analyzed by the Pearson correlation coefficient. The dry and fresh weight, yield per plant and fruit quality of the second ear were measured. The comprehensive quality of tomato was evaluated and ranked by PCA method and combined weighting-TOPSIS method based on game theory.【Result】The effects of increasing row spacing on canopy leaf area, light interception and photosynthetic capacity were mainly reflected in the middle and lower parts of the canopy. The leaf area in the mid canopy increased first and then decreased with the increase of the row spacing. The leaf area in the lower canopy and the light interception in the mid and lower canopy increased significantly from P1 to P2, but slightly increased from P2 to P3; the Pn in the mid and lower canopy showed that P2 increased by 8.06%-11.32% compared with P1, and P3 increased by 14.25%-24.40% compared with P2; the LMA showed that P2 increased by 1.31%-33.24% compared with P1, and P3 increased by 6.09%-17.86% compared with P2; the Chl content of P2 was 3.42%-6.81% higher than that of P1, and P3 was 3.19%-4.96% higher than that of P2; the N content of P2 was 13.89%-34.73% higher than that of P1, and P3 was 2.21%-19.74% higher than that of P2; the content of P and K had no obvious regularity. On the whole, the content of Pn, Chl and N increased with the increase of row spacing, and the LMA increased with the increase of row spacing under light deficit irrigation and showed P3>P1>P2 under conventional irrigation; under three row spacing levels, the LMA and N content under conventional irrigation were higher than those under light deficit irrigation, the Pn under conventional irrigation was higher than that under light deficit irrigation under P1 and P3, while the Pn under light deficiency irrigation was higher under P2; the Chl content under conventional irrigation was higher under P1, while the Chl content under light deficiency irrigation was higher under P2 and P3. With the increase of row spacing, the dry and fresh weight of the aboveground parts increased under conventional irrigation, and increased first and then decreased under light deficit irrigation; the aboveground dry and fresh weight of conventional irrigation was higher than that of light deficit irrigation. The yield per plant increased with the increase of row spacing under the two irrigation levels, and the increase from P1 to P2 was larger (compared with P1, P2 under conventional irrigation and light deficit irrigation increased by 33.75% and 24.32%, respectively.), while the yield per plant increased only slightly from P2 to P3 (compared with P2, P3 increased by 2.87% and 4.30% under conventional irrigation and light deficit irrigation, respectively.); the yield per plant under conventional irrigation was higher than that under light deficit irrigation. Increasing row spacing and reducing irrigation amount could optimize the comprehensive quality of fruit, and the top three comprehensive quality scores were P3W2, P2W2 and P3W1.【Conclusion】P3W1 was the highest in leaf Pn, LMA, N content, aboveground dry and fresh weight and yield per plant, and P3W2 was the highest in canopy light interception, Chl content and comprehensive quality score.

    Comparison of Sugar Content and Expression Analysis of Genes Related to Sugar Metabolism in Different Parts of Chinese Flowering Cabbage
    FENG XianJun, WANG Li, WANG Tong, HOU LeiPing, LI MeiLan
    Scientia Agricultura Sinica. 2023, 56(11):  2158-2171.  doi:10.3864/j.issn.0578-1752.2023.11.010
    Abstract ( 279 )   HTML ( 12 )   PDF (2339KB) ( 2216 )   Save
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    【Objective】 Chinese flowering cabbage has become one of the most popular vegetables in southern China due to its crunchy taste and high nutritional value. The type and content of sugars are the main factors for determining the sweetness and flavour of Chinese flowering cabbage. Therefore, this paper analyzed the sugar content and the expression of genes related to sugar metabolism and transport in different organs, so as to investigate the molecular mechanisms responsible for the differences in sugar content in different organs of Chinese flowering cabbage. 【Method】 In this experiment, the content of soluble sugars, sucrose, glucose and fructose in leaves, stalks and flower buds were determined by high performance liquid chromatography (HPLC). The expression of genes in leaves, stalks and buds was analyzed comprehensively and systematically by transcriptome sequencing (RNA-seq), and the differentially expressed genes (DEGs) between three different organs were identified using DESeq2 software. In addition, the expression of genes encoding enzymes involved in the sugar metabolic pathway and transport process was analyzed, and the correlation with sugar content were analyzed on OmicShare. Moreover, the expression of some genes was verified using quantitative real-time fluorescence PCR (qRT-PCR).【Result】The sugar content varied significantly among the different organs of Chinese flowering cabbage. Soluble sugars, sucrose, glucose and fructose all showed a trend of buds>stalks>leaves. The glucose content in flower buds was 1.3 and 1.6 times of that in stalks and leaves; the fructose content was 1.42 and 1.78 times of that in stalks and leaves, respectively. By analyzing the relation and consistency between the expression of genes coding enzymes involving in sugar metabolism and transport and content of the sugar, a total of 18 genes were screened. By analyzing the correlation and consistency of the expression of DEGs among different organs with sugar species and content, 14 DEGs were identified. By merging 7 common genes (Bra020096, Bra029914, Bra033419, Bra037980, Bra027398, Bra006129 and Bra006130), expression of 25 genes in total were consistent with the sugar content. qRT-PCR results showed that the relative expression of the eight DEGs were highly consistent with their FPKM values from RNA-seq, indicating that the sequencing results were accurate and reliable.【Conclusion】 The sugar content in the flower buds of Chinese flowering cabbage was higher than that in stalks and leaves, because the expression of genes encoding sugar synthesis-related enzymes was higher in buds than in stalks and leaves. These results laid a certain foundation for revealing the molecular mechanism of sugar metabolism and transport in different organs of Chinese flowering cabbage.

    FOOD SCIENCE AND ENGINEERING
    Effects of nuoB on the Biofilm Formation and Cellular Metabolism of Meat-Borne Pseudomonas fragi During Chilled Storage
    WU YaJie, TAN Song, CHEN YuPing, NIU AJuan, LIU YuXin, WANG GuangYu, XU XingLian, QIU WeiFen
    Scientia Agricultura Sinica. 2023, 56(11):  2172-2185.  doi:10.3864/j.issn.0578-1752.2023.11.011
    Abstract ( 223 )   HTML ( 14 )   PDF (2771KB) ( 652 )   Save
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    【Objective】This paper focused on the impacts of nuoB on the biofilm formation and cell metabolism in Pseudomonas fragi (P. fragi), so as to further reveal the regulatory mechanism of nuoB in the spoilage of chilled meat contaminated with P. fragi, and to provide a theoretical basis for developing effective preservation system of chilled meat. 【Method】P. fragi NMC25 and its nuoB-mutant strain were used in the present study, and the differences in the spatial structure of biofilms were observed by confocal laser scanning microscopy (CLSM). The changes in biofilm composition were tested by the cell enumeration and the analysis of the extracellular polymeric substances. In addition, the ultra-high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS) was employed to investigate the alterations of nuoB-related metabolite abundance. 【Result】CLSM images showed that cells in the biofilms of wild-type strains cultivated in situ was highly dense, nematic ordering, whereas ΔnuoB displayed relatively disorganized and sparse arrangement. Additionally, cell enumeration revealed insignificant differences between the wild-type and mutant biofilms regardless of the difference of culture medium. The result indicated that the mutants did not change significantly in their ability to grow as biofilm on the surface of TSA or meat sample. For extracellular polymeric substances from biofilms in situ, the protein and carbohydrate contents of ΔnuoB were significantly higher (P<0.01, P<0.05, respectively) than those of wild-type strains, indicating that nuoB affected the secretion of extracellular polymers by P. fragi. The metabolomics results revealed a clear separation between the wild and mutant groups in an orthogonal partial least squares discriminant analysis model (R2X=0.481, R2Y=0.977, Q2=0.909), which suggested that the metabolites of the mutants had changed markedly. In the model, differentially expressed metabolites were screened, including 2-hydroxycinnamic acid, L-tyrosine, L-phenylalanine, DL-tryptophan, 17(S)-HETE, and 5-OxoETE. Pathway mapping analysis was conducted based on the chosen candidates. In total, the major metabolic pathways included fatty acid biosynthesis, unsaturated fatty acid biosynthesis, riboflavin metabolism, 2-oxocarboxylic acid metabolism, purine metabolism, cyanogenic amino acid metabolism, and phenylalanine metabolism. 【Conclusion】The disruption of nuoB stimulated significant variations in the spatial structure of the P. fragi biofilm grown in situ, promoting the biosynthesis of extracellular polymeric substances and affecting intracellular metabolic pathways, such as carbon, nucleotide, lipid, and amino acid metabolism.

    ANIMAL SCIENCE·VETERINARY SCIENCE
    Effects of Pladienolide B on Expression of Pluripotency Related Genes and Cell Viability of Bovine Embryonic Stem Cells
    ZHAO Fang, DING Qiang, XIA ShuWen, GAO YunDong, LAN GuoCheng, LIN ZhiPing, WANG HuiLi, ZHONG JiFeng
    Scientia Agricultura Sinica. 2023, 56(11):  2186-2201.  doi:10.3864/j.issn.0578-1752.2023.11.012
    Abstract ( 237 )   HTML ( 15 )   PDF (6401KB) ( 65 )   Save
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    【Background】 Due to high pluripotency of bovine embryonic stem cells (BESCs), they have important application values in cattle breed conservation, breed selection, and regulation mechanism study of livestock embryo development. However, the studies on the maintenance of pluripotency and differentiation of BESCs are limited, and the regulative mechanism remains unclear. 【Objective】The aim of this study was to investigate the effects of different concentrations of Pladienolide B (PlaB) on the expression of pluripotent markers, totipotent markers and embryonic cell-lineage genes as well as the cell viability of BESCs, so as to provide the reference and theoretical basis for improving the developmental potency of BESCs.【Method】Immunofluorescence was used to detect the expression of pluripotent markers of bovine BESCs, and real-time fluorescence quantitative PCR (RT-qPCR) was used to detect the effects of different concentrations of PlaB on the expression of spliceosome, totipotent markers and embryonic cell-lineage genes of BESCs. RT-qPCR and Western Blot were used to detect the effects of different concentrations of PlaB on both mRNA and protein expression of pluripotent markers of BESCs. CCK8 and EDU staining was used to detect the effects of different concentrations of PlaB on the proliferation of BESCs. 【Result】 RT-qPCR results showed that the mRNA expression levels of SF3B1 and SF3B2 in EPSCM-BESCs were significantly down-regulated by 0.5 nmol·L-1 to 1.5 nmol·L-1 PlaB; when the PlaB concentration was 1.5 nmol·L-1, the mRNA expressions of SF3B1 and SF3B2 in CTFR-BESCs were decreased; when the PlaB concentration ranged from 0.5 nmol·L-1 to 1.5 nmol·L-1, the mRNA expression levels of SF3B4 and SF3B5 in both CTFR-BESCs and EPSCM-BESCs were increased in a dose-dependent manner. Furthermore, PlaB significantly down-regulated mRNA expression of SF3B6 in the CTFR-BESCs. When PlaB concentration ranged from 0.5 nmol·L-1 to 1.5 nmol·L-1, the mRNA expression of spliceosome LSM4 both in EPSCM-BESCs and CTFR-BESCs were significantly down-regulated. The concentration from 0.5 nmol·L-1 to 1.5 nmol·L-1 PlaB significantly down-regulated the expression levels of EFTUD2 mRNA in CTFR-BESCs; the mRNA expression of EFTUD2 mRNA was significantly down-regulated in BEPSCM-BESCs with 1 nmol·L-1 and 1.5 nmol·L-1 PlaB while PlaB concentration from 0.5 to 1.5 nmol·L-1, both the mRNA expression and protein levels of the pluripotent markers OCT4, SOX2 and NANOG in CTFR-BESCs and EPSCM-BESCs were up-regulated in a dose-dependent manner. By the concentration range from 0.5 to 1.5 nmol·L-1, PlaB dose-dependently up-regulated the mRNA levels of totipotent markers such as MDM2, PID1 and BTG2 in CTFR-BESCs and EPSCM-BESCs, while the mRNA levels of DDIT4 and PDRG1 were down-regulated. The mRNA expression of embryonic cell lineage genes in the CTFR-BESCs were up-regulated while the PlaB was added. The addition of PlaB in EPSCM-BESCs significantly reduced the expression of GATA4, GATA6, SOX7 and other embryonic cell lineage genes, but had no significant effect on ZIC1. The cell viability of CTFR-BESCs and EPSCM-BESCs showed a downward trend with increasing of PlaB dose and treatment time, while CTFR-BESCs was more sensitive than EPSCM-BESCs. 【Conclusion】PlaB significantly up-regulated the expression of pluripotent markers and partial totipotent markers in CTFR-BESCs and EPSCM-BESCs, and the expression of gene lineages and cell viability in EPSCM-BESCs were decreased. The effective concentration and effects on gene expression of PlaB in the two types BESCs were not completely consistent. Due to the inhibiting effect of PlaB on cell viability of BESCs, the further studies were needed to optimize the culture system.

    The Micro-Structure of Tibetan Sheep Lung and Its HIF-1α and AQP1 Expression Characteristics
    AYIMUGULI Abudureyimu, ZHANG Chen, CAI Yong, QIN Sheng, LUO WenXue, ZHAXIYINGPAI
    Scientia Agricultura Sinica. 2023, 56(11):  2202-2211.  doi:10.3864/j.issn.0578-1752.2023.11.013
    Abstract ( 256 )   HTML ( 13 )   PDF (3804KB) ( 648 )   Save
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    【Background】HIF-1α is one of the key factors for cells to make adaptive response to hypoxia stress. It mainly maintains the balance of oxygen supply by regulating gene transcription. Aquaporins (AQPs) are hydrophobic transmembrane transporters regulating water homeostasis. Among them, AQP-1 mainly regulates the water transport among alveoli, pulmonary interstitium and capillaries, so as to maintain the fluid balance in the lung. The plateau environment is characterized by low oxygen, extremely cold, strong wind and radiation, so there are relatively few species which could adapt and survive. As the unique sheep species adapt to high altitude and high cold climate, Tibetan sheep has formed a special morphological structure and physiological functions adapted to the plateau environment. As the main executive organ of respiration, lung is very sensitive to hypoxia. 【Objective】 This study aimed to explore the structural characteristics of Tibetan sheep lung and the expression characteristics of HIF-1α and AQP1, so as to reveal the related roles of HIF-1α and AQP1 in Tibetan sheep high altitude adaption. 【Method】 The histochemical HE, PAS and Masson staining, immuno-histochemical SP and real-time fluorescence quantitative were used.【Result】The tunica thickness of lung in Tibetan sheep was 40.28 μm, which showed no significant difference with that of Small Tail Han sheep, but the elastic fiber proportion was significantly higher than that of Small Tail Han sheep (P<0.05); the number of goblet cells in bronchiolar epithelium in Tibetan sheep was significantly more than that in Small Tail Han sheep (P<0.05), but there was no significant difference in superior branches; some goblet cells were still detected in the epithelium of bronchioli terminales of Tibetan sheep; the thickness of smooth muscle of bronchioli terminales of Tibetan sheep was significantly thicker than that of Small Tail Han sheep (P<0.05); the proportion of smooth muscle in the pulmonary arteriole (diameter less than 100 μm) in Tibetan sheep was significantly less than that of Small Tail Han sheep; the thickness of respiratory bronchiole smooth muscle and the number of capillaries in Tibetan sheep were significantly higher than those in Small Tail Han sheep. The HIF-1α protein was mainly expressed in bronchiolar epithelium, pulmonary micro-vascular endothelium and alveolar septum, and mainly detected in cytoplasm; both Tibetan sheep and Small Tail Han sheep, the expression of HIF-1α was significantly higher in high altitude than that of low altitude, and HIF-1α expression in low altitude Tibetan sheep was significantly stronger than that of Small Tail Han sheep living at low altitude. AQP1 protein was mainly expressed in alveolar epithelium, alveolar septum, pulmonary microvascular endothelium, submucosal and smooth muscle of bronchioles, mainly located on cell membrane; the expression of AQP1 in lung of Tibetan sheep living at high altitude was significantly stronger than that of Tibetan sheep from low altitude, and also stronger than that of Small Tail Han sheep either altitude (P<0.05), but there was no significant difference between Small Tail Han sheep living at high altitude and low (P>0.05).【Conclusion】All those results indicated that the lung capsule of Tibetan sheep contained more elastic fibers and less microvascular smooth muscle than that of Small Tail Han sheep, with more number of goblet cells and smooth muscle in respiratory bronchioles. The expression of HIF-1α and AQP1 in Tibetan Sheep lung were significantly stronger than that of Small Tail Han sheep, and their expression increased with living altitude increasing.

    Development Strategies and Application Prospects of African Swine Fever Vaccines: Feasibility and Probability
    WANG Tao, LUO Rui, SUN Yuan, QIU HuaJi
    Scientia Agricultura Sinica. 2023, 56(11):  2212-2222.  doi:10.3864/j.issn.0578-1752.2023.11.014
    Abstract ( 383 )   HTML ( 29 )   PDF (572KB) ( 2197 )   Save
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    African swine fever (ASF) is a fatal hemorrhagic disease in domestic pigs caused by African swine fever virus (ASFV) and the mortality of acute ASF is as high as 100%. Since ASF was introduced to China in 2018, the global prevalence and impacts of ASF were increasing. After more than three years of the epidemic of the virulent genotype II ASFV, the low virulent genotypes II and I ASFV strains emerged, resulting in an extremely complicated situation for the control and eradication of ASF in China. As an effective tool for the prevention and control of infectious diseases, the research and development of ASF vaccines have received considerable attention from the government, pig industry, vaccine manufacturers, and the scientific community. With the continuous investment of ASF research funding in recent years, based on development strategies of inactivation, attenuation, subunit, viral vector, and DNA vaccines, the unprecedented progress has been made in the development of ASF vaccines, which increased our understanding of ASF vaccine development and evaluation. In addition, some promising ASF vaccine candidates have been evaluated in preclinical models and were currently in clinical trials, showing the good application prospects, but there were still some problems hindering its further development. With a focus on the latest advances in ASF vaccines, this review summarized the advantages and disadvantages of different vaccines development strategies, the comprehensive evaluation of current ASF vaccine candidates, the development direction in the future, the challenges in developing safe and efficacious ASF vaccines, and the application prospects of ASF vaccines, so as to provide some insights for industry insiders.

    RESEARCH NOTES
    The Modification of Gene Editing Vector for Efficient GFPuv Fluorescence Screening and Its Application in Potato Genetic Transformation
    DU JingYa, CHEN KaiYuan, PU Jin, ZHOU HuiYing, ZHU GuangTao, ZHANG ChunZhi, DU Hui
    Scientia Agricultura Sinica. 2023, 56(11):  2223-2236.  doi:10.3864/j.issn.0578-1752.2023.11.015
    Abstract ( 380 )   HTML ( 35 )   PDF (3831KB) ( 2063 )   Save
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    【Objective】The improvement and innovation of screening markers contributes to the development of transgenic technology, among which the visual screening markers are widely modified for better effect. Recent studies revealed that an enhanced Yellow Green Fluorescent like Protein (eYGFPuv (GFPuv)) obtained by mutation can emit strong and stable green fluorescence under 365 nm UV light irradiation and be easily observed. Constructing the gene editing vector with GFPuv fluorescence screening marker and carrying out experiment application and verifications in potato genetic transformation will provide technical support for the screening of positive transgenic plants in potato transformation, and lay the foundation for using genome editing technology to create potato male sterile lines in the future. 【Method】By using homologous recombination, the GFPuv expression framework and gene editing element Cas9_sgRNA were successively recombined into pCAMBIA2300 vector, and then with this new designed vector the Agrobacterium-mediated transient expression assay was conducted in tobacco plants. Six editing vectors with potato anther development conservative genes were constructed using this modified vector. The A. rhizogenes strains Ar qual and MSU440 harbouring these vectors were transformed into the potato stem segments respectively, and then the A. rhizogenes-induced hairy roots with green fluorescence were observed and counted under the portable UV lamp. The transformation efficiency and editing efficiency of these vectors were analyzed using hairy root transformation system in two different potato genotypes. In the end, the modified vectors were applied to produce transformed potato plants with modifications on target genes. 【Result】A novel gene editing vector pCAMBIA2300MGFPuv-sgRNACas harbouring a GFPuv fluorescence marker was successfully constructed, and the transient transformation in tobacco plants confirmed that the GFPuv expression framework was expressed successfully. The hairy roots with green fluorescence were screened after the transformation with two kinds of A. rhizogenes, and an additional supplement of kanamycin (Kan) significantly increased the proportion of positive fluorescent roots. Although the transformation rates of the two strains were not significantly different, the hairy roots of MSU440 formed faster. Furthermore, the transformation rates and editing rates of editing vectors for six potato anther development conservative genes in two different potato genotypes were the same, but the editing rates of six target sites differed significantly. Potato genetic transformation using the modified vector confirmed that GFPuv fluorescence could be used for the screening of transgenic callus and plants in potato. 【Conclusion】The hairy root transformation system mediated by A. rhizogenes is an essential approach to verifying the efficiency of gene editing, and GFPuv fluorescence can be used in the screening of transgenic plants in potato transformation.