Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (22): 4658-4666.doi: 10.3864/j.issn.0578-1752.2020.22.012

• HORTICULTURE • Previous Articles     Next Articles

Effects of 1-MCP and Delayed Cold-Storage on Quality and Expression of Softening Related Genes in ‘Doyenne du Comice’ Pear During Shelf-Life

CHENG YuDou,ZHANG YaGuang,GUAN JunFeng(),FENG YunXiao,HE JinGang   

  1. Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences/Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051
  • Received:2020-03-07 Accepted:2020-05-19 Online:2020-11-16 Published:2020-11-28
  • Contact: JunFeng GUAN E-mail:junfeng-guan@263.net

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Abstract:

【Objective】Focusing on the serious loss of ‘Doyenne du Comice’ (Pyrus communis L.) pear fruit that was resulted from rapid softening and decay, the effects of 1-methylcyclopropene (1-MCP) and delayed cold-storage on fruit decay and softening at shelf-life was studied, and the expression pattern of the softening-related genes of ‘Doyenne du Comice’ pear was also detected, so as to provide a theoretical basis for extending the shelf-life of ‘Doyenne du Comice’ pear.【Method】The ‘Doyenne du Comice’ pear fruit were firstly treated with 1.0 μL?L -1 1-MCP, and then were respectively placed at 20 ± 2℃ for 0, 3 and 6 d before storing at (0 ± 0.5)℃, which was considered as the delayed cold-storage. After 95 d of cold storage, the fruit quality was detected at shelf-life (20 ± 2℃), and the expression patterns of softening associated genes, such as polygalacturonase genes (PG1, PG2), α-arabinofuranosidase genes (ARF1, ARF2) and β-galactosidase gene (GAL4), were studied by the real-time quantitative PCR (qRT-PCR).【Result】The delayed cold-storage treatment could easily lead to fruit softening and decay in ‘Doyenne du Comice’ pear, and the fruit decay rate increased with the extending of delayed cold-storage. Compared with the treatment that the fruit were directly stored at 0 °C after air sealing (CK), the treatments of 1-MCP and 1-MCP combined with delayed cold-storage could maintain a higher fruit firmness, and reduce the rates of fruit decay, respiration and ethylene production, but it would increase in the rates of respiration and ethylene production, and accelerate the fruit softening with the extending of delayed cold-storage. The expression of softening related genes, such as PG1, PG2, ARF1, ARF2 and GAL4, were significantly inhibited by 1-MCP and 1-MCP combined with delayed cold-storage, while it would be weakened with the delayed cold-storage extended.【Conclusion】The decay of ‘Doyenne du Comice’ pear fruit was easily induced by delayed cold-storage treatment, so it was proposed that the postharvest fruit should be rapidly stored under the cold condition. The inhibition of the fruit softening and decay by 1-MCP treatment was more obvious, and the treatment that 1-MCP combined with delayed cold-storage was beneficial to fruit softening. The genes of PG1, PG2, ARF1 and GAL4 were involved in the softening of ‘Doyenne du Comice’ pear at shelf-life after cold storage.

Key words: ‘Doyenne du Comice’ pear;, 1-methylcyclopropene, delayed cold-storage, quality, softening

Table 1

Primers of the fruit softening-related gene for qRT-PCR"

基因
Gene		 登录号
Access No.		 正向引物
Forward primer		 反向引物
Reverse primer
PG1 KC855751 5′-GCTTATCCTTCATACATGGACACC-3′ 5′-CTGATGATGTTGCTATCCCACC-3′
PG2 KC855752 5′-AAATCAGAAAATCCTAAAACCAGC-3′ 5′-GCTTATAGCCATCATCAACAACG-3′
ARF1 KC855732 5′-CTTTTTTCCACAATCCTCATTCC-3′ 5′-GCTCTCGACGGTATAGAATTTGC-3′
ARF2 KC855733 5′-TAGCAACATTCCCATCCACC-3′ 5′-AACATTACACTGCCTATGATCTCG-3′
GAL4 KC855745 5′-GATCAATTCACTATCCCAGAAGC-3′ 5′-GTATAACATCCAAGCCTCCAGC-3′
ACT2 GU830958 5′-GGACATTCAACCCCTCGTCT-3′ 5’-ATCCTTCTGACCCATACCAACC-3’

Table 2

Effect of 1-MCP and delayed cold-storage on the decay of 'Doyenne du Comice' pear fruit"

处理 Treatment 腐烂率 Decay rate (%)
CK 8.8±2.4c
1-MCP 1.3±2.2d
20℃ 3 d 33.7±0.6b
1-MCP+20℃ 3 d 8.7±2.1c
20℃ 6 d 91.3±5.6a
1-MCP+20℃ 6 d 6.3±2.2cd

Fig. 1

Effects of 1-MCP and delayed cold-storage treatments on the firmness (A), SSC (B) and TA content (C) of 'Doyenne du Comice' pear during shelf-life Different small letters in the figure indicated significant differences (P<0.05). The same as below"

Fig. 2

Effects of 1-MCP and delayed cold-storage treatments on the rates of respiration (A) and ethylene production (B) of 'Doyenne du Comice' pear during shelf-life"

Fig. 3

Effects of 1-MCP and delayed cold-storage treatments on the expression of PGs in the 'Doyenne du Comice' pear during shelf-life"

Fig. 4

Effects of 1-MCP and delayed cold-storage treatments on the expression of ARFs (A and B) in the 'Doyenne du Comice' pear during shelf-life"

Fig. 5

Effects of 1-MCP and delayed cold-storage treatments on the expression of GAL4 in the 'Doyenne du Comice' pear during shelf-life"

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