1-MCP和延迟冷藏对‘早红考密斯’梨货架期间品质 和软化相关基因表达的影响

doi:10.3864/j.issn.0578-1752.2020.22.012

Abstract

Abstract:

【Objective】Focusing on the serious loss of ‘Doyenne du Comice’ (Pyrus communis L.) pear fruit that was resulted from rapid softening and decay, the effects of 1-methylcyclopropene (1-MCP) and delayed cold-storage on fruit decay and softening at shelf-life was studied, and the expression pattern of the softening-related genes of ‘Doyenne du Comice’ pear was also detected, so as to provide a theoretical basis for extending the shelf-life of ‘Doyenne du Comice’ pear.【Method】The ‘Doyenne du Comice’ pear fruit were firstly treated with 1.0 μL?L ^-1 1-MCP, and then were respectively placed at 20 ± 2℃ for 0, 3 and 6 d before storing at (0 ± 0.5)℃, which was considered as the delayed cold-storage. After 95 d of cold storage, the fruit quality was detected at shelf-life (20 ± 2℃), and the expression patterns of softening associated genes, such as polygalacturonase genes (PG1, PG2), α-arabinofuranosidase genes (ARF1, ARF2) and β-galactosidase gene (GAL4), were studied by the real-time quantitative PCR (qRT-PCR).【Result】The delayed cold-storage treatment could easily lead to fruit softening and decay in ‘Doyenne du Comice’ pear, and the fruit decay rate increased with the extending of delayed cold-storage. Compared with the treatment that the fruit were directly stored at 0 °C after air sealing (CK), the treatments of 1-MCP and 1-MCP combined with delayed cold-storage could maintain a higher fruit firmness, and reduce the rates of fruit decay, respiration and ethylene production, but it would increase in the rates of respiration and ethylene production, and accelerate the fruit softening with the extending of delayed cold-storage. The expression of softening related genes, such as PG1, PG2, ARF1, ARF2 and GAL4, were significantly inhibited by 1-MCP and 1-MCP combined with delayed cold-storage, while it would be weakened with the delayed cold-storage extended.【Conclusion】The decay of ‘Doyenne du Comice’ pear fruit was easily induced by delayed cold-storage treatment, so it was proposed that the postharvest fruit should be rapidly stored under the cold condition. The inhibition of the fruit softening and decay by 1-MCP treatment was more obvious, and the treatment that 1-MCP combined with delayed cold-storage was beneficial to fruit softening. The genes of PG1, PG2, ARF1 and GAL4 were involved in the softening of ‘Doyenne du Comice’ pear at shelf-life after cold storage.

Key words: ‘Doyenne du Comice’ pear;, 1-methylcyclopropene, delayed cold-storage, quality, softening

CHENG YuDou,ZHANG YaGuang,GUAN JunFeng,FENG YunXiao,HE JinGang. Effects of 1-MCP and Delayed Cold-Storage on Quality and Expression of Softening Related Genes in ‘Doyenne du Comice’ Pear During Shelf-Life[J].Scientia Agricultura Sinica, 2020, 53(22): 4658-4666.

Figures/Tables 7

Table 1

Table 2

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

References 35

[1]	祝美云, 李小月, 梁丽松, 马庆华, 王贵禧 . 1-MCP处理和打蜡处理对‘早红考密斯’西洋梨贮藏期和货架期保护性酶活性的影响. 食品科学 2015,36(24):307-312. doi: 10.7506/spkx1002-6630-201524057
	ZHU M Y, LI X Y, LIANG L S, MA Q H, WANG G X . Effect of 1-Methylcyclopropene (1-MCP) and wax treatments on the activities of protective enzymes in Pyrus communis L. cv. ‘Doyenne du Comice’ during cold storage and shelf life. Food Science, 2015,36(24):307-312. (in Chinese) doi: 10.7506/spkx1002-6630-201524057
[2]	JOHNSTON J W, HEWETT E W, HERTOG M L . Apple (Malus domestica) softening in the postharvest coolchain: Effects of delayed cooling and shelf-life temperatures. New Zealand Journal of Crop and Horticultural Science, 2005,33(3):283-292. doi: 10.1080/01140671.2005.9514361
[3]	DELONG J M, PRANGE R K, HARRISON P A . The influence of pre-storage delayed cooling on quality and disorder incidence in ‘Honeycrisp’ apple fruit. Postharvest Biology and Technology, 2004,34(3):353-358. doi: 10.1016/j.postharvbio.2004.10.001
[4]	DEELL J R, EHSANI-MOGHADDAM B . Timing of postharvest 1-methylcyclopropene treatment affects Bartlett pear quality after storage. Canadian Journal of Plant Science, 2011,91(5):853-858. doi: 10.4141/CJPS2011-060
[5]	VILLALOBOS-ACUÑA M, MITCHAM E J. Ripening of European pears: The chilling dilemma. Postharvest Biology and Technology, 2008, 49(2):187-200. doi: 10.1016/j.postharvbio.2008.03.003
[6]	CRISOSTO C H, GARNER D, CRISOSTO G M, SIBBETT S, DAY K R . Late harvest and delayed cooling induce internal browning of ‘Ya Li’ and ‘Seuri’ Chinese pears. Hortscience, 1994,29(4):667-670. doi: 10.21273/HORTSCI.29.6.667
[7]	KOUTSOFLINI A, GERASOPOULOS D, VASILAKAKIS M . The effects of fruit maturation, delayed storage and ethylene treatment on the incidence of low-temperature breakdown of ‘Hayward’ kiwifruit. Journal of the Science of Food and Agriculture, 2012,93(2):410-414. doi: 10.1002/jsfa.5776 pmid: 22777890
[8]	MANGANARIS G A, VICENTE A R, CRISOSTO C H, LABAVITCH J M . Effect of delayed storage and continuous ethylene exposure on flesh reddening of ‘Royal Diamond’ plums. Journal of the Science of Food and Agriculture, 2008,88(12):2180-2185. doi: 10.1002/jsfa.v88:12
[9]	SISLER E C, SEREK M . Inhibitors of ethylene responses in plants at the receptor level: Recent developments. Physiologia Plantarum, 1997,100(3):577-582. doi: 10.1111/ppl.1997.100.issue-3
[10]	WATKINS B C . The use of 1-methylcyclopropene (1-MCP) on fruits and vegetables. Biotechnology Advances, 2006,24(4):389-409. doi: 10.1016/j.biotechadv.2006.01.005
[11]	冯云霄, 何近刚, 程玉豆, 李丽梅, 李学营, 冯建忠, 关军锋 . 1-MCP处理对早熟苹果常温贮藏生理及品质的影响. 现代食品科技, 2019,35(12):130-136.
	FENG Y X, HE J G, CHENG Y D, LI L M, LI X Y, FENG J Z, GUAN J F . Effects of 1-MCP treatment on the physiology and quality of early-maturing apples stored at ambient temperature. Modern Food Science and Technology, 2019,35(12):130-136. (in Chinese)
[12]	姜云斌, 佟伟, 王志华, 王文辉, 都兴日, 夏玉静 . 1-MCP处理对明珠、贵妃梨货架期间品质的影响. 中国农学通报 2010,26(21):67-70.
	JIANG Y B, TONG W, WANG Z H, WANG W H, DU X R, XIA Y J . Effects of 1-MCP treatment on shelf-life quality of Mingzhu pear and Guifei pear. Chinese Agricultural Science Bulletin, 2010,26(21):67-70. (in Chinese)
[13]	王晓飞, 杨艳青, 任小林, 孙海亭, 向春燕, 孙炜尚 . 1-MCP对‘粉红女士’苹果果实采后生理及其品质的影响. 食品科学, 2014,35(18):219-223. doi: 10.7506/spkx1002-6630-201418042
	WANG X F, YANG Y Q, REN X L, SUN H T, XIANG C Y, SUN W S . Effects of 1-MCP on postharvest physiology and quality of ‘Pink Lady’ apple fruits. Food Science, 2014,35(18):219-223. (in Chinese) doi: 10.7506/spkx1002-6630-201418042
[14]	ARGENTA L C, MATTHEIS J P, FAN X, AMARANTE C V T. Managing ‘Bartlett’ pear fruit ripening with 1-methylcyclopropene reapplication during cold storage. Postharvest Biology and Technology, 2016,113:125-130. doi: 10.1016/j.postharvbio.2015.11.009
[15]	SAQUET A A, ALMEIDA D P F. Ripening physiology and biochemistry of ‘Rocha’ pear as affected by ethylene inhibition. Postharvest Biology and Technology, 2017,125:161-167. doi: 10.1016/j.postharvbio.2016.11.008
[16]	CHENG Y D, LIU L Q, FENG Y X, DONGY , GUAN J F,. Effects of 1-MCP on fruit quality and core browning in ‘Yali’ pear during cold storage. Scientia Horticulturae, 2019,243:350-356. doi: 10.1016/j.scienta.2018.08.041
[17]	TAO D B, WANG J W, ZHANG L, JIANG Y G, LV M . 1-Methylcyclopropene alleviates peel browning of ‘Nanguo’ pears by regulating energy, antioxidant and lipid metabolisms after long term refrigeration. Scientia Horticulturae, 2018,247:254-263. doi: 10.1016/j.scienta.2018.12.025
[18]	MWANIKI M W, MATHOOKO F M, MATSUZAKI M, HIWASA K, TATEISHI A, USHIJIMA K, NAKANO R, INAB A A, KUBO Y . Expression characteristics of seven members of the β-galactosidase gene family in ‘La France’ pear(Pyrus communis L.) fruit during growth and their regulation by 1-methylcyclopropene during postharvest ripening. Postharvest Biology and Technology, 2005,36(3):253-263. doi: 10.1016/j.postharvbio.2005.02.002
[19]	SEKINE D, MUNEMURA I, GAO M, MITSUHASHI W, TOYOMASU T, HIDEKI M . Cloning of cDNAs encoding cell-wall hydrolases from pear ( Pyrus communis) fruit and their involvement in fruit softening and development of melting texture. Physiologia Plantarum, 2006,126(2):163-174. doi: 10.1111/ppl.2006.126.issue-2
[20]	FONSECA S, MONTEIRO L, BARREIRO M G, PAIS M S . Expression of genes encoding cell wall modifying enzymes is induced by cold storage and reflects changes in pear fruit texture. Journal of Experimental Botany, 2005,56(418):2029-2036. doi: 10.1093/jxb/eri201 pmid: 15955791
[21]	SONG L Y, WANG Z G, WANG Z M, MENG G, ZHAI R, CAI M, MA F W, XU L F . Screening of cell wall-related genes that are expressed differentially during ripening of pears with different softening characteristics. Postharvest Biology and Technology, 2016,115:1-8. doi: 10.1016/j.postharvbio.2015.12.012
[22]	WEI J M, QI X D, CHENG Y D, GUAN J F . Difference in activity and gene expression of pectin-degrading enzymes during softening process in two cultivars of Chinese pear fruit. Scientia Horticulturae, 2015,197:434-440. doi: 10.1016/j.scienta.2015.10.002
[23]	WEI J M, CHENG Y D, FENG Y X, QI X D, HE J G, GUAN J . Different response to 1-methylcyclopropene in two cultivars of Chinese pear fruit with contrasting softening characteristics. Journal of Applied Botany and Food Quality, 2019,92:138-142.
[24]	GASIC K, HERNANDEZ A, KORBAN S S . RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction. Plant Molecular Biology Reporter, 2004,22(4):437-438. doi: 10.1007/BF02772687
[25]	PECH J, PURGATTO E, BOUZAYEN M, LATCHÉ A . Ethylene and fruit ripening. Annual Plant Reviews, 2012,44:275-304.
[26]	XIE X B, ZHAO J, WANG Y . Initiation of ripening capacity in 1-MCP treated green and red ‘Anjou’ pears and associated expression of genes related to ethylene biosynthesis and perception following cold storage and post-storage ethylene conditioning. Postharvest Biology and Technology, 2016,111:140-149. doi: 10.1016/j.postharvbio.2015.08.010
[27]	TRINCHERO G D, SOZZI G O, COVATTA F, FRASCHINA A A . Inhibition of ethylene action by 1-methylcyclopropene extends postharvest life of ‘Bartlett’ pears. Postharvest Biology and Technology, 2004,32(2):193-204. doi: 10.1016/j.postharvbio.2003.11.009
[28]	贾晓辉, 李振茹, 王文辉, 佟伟, 夏玉静, 张志云, 王志华 . 1-MCP处理对巴梨果实采后生理效应及黑皮病的影响. 保鲜与加工, 2010,10(4):13-16.
	JIA X H, LI Z R, WANG W H, TONG W, XIA Y J, ZHANG Z Y, WANG Z H . Influence of 1-Methylcyclopropene on postharvest physiology and skin brown of ‘Bartlett’ Pears. Storage and Process, 2010,10(4):13-16. (in Chinese)
[29]	ARGENTA L C, FAN X, MATTHEIS J P . Influence of 1- methylcyclopropene on ripening, storage life, and volatile production by d’Anjou cv. pear fruit. Journal of Agricultural and Food Chemistry, 2003,51(13):3858-3864. doi: 10.1021/jf034028g pmid: 12797756
[30]	祝美云, 李小月, 梁丽松, 马庆华, 王贵禧 . 不同采后处理对‘阿巴特’西洋梨果实冷藏后货架期品质的影响. 中国农学通报, 2015,31(33):273-278.
	ZHU M Y, LI X Y, LIANG L S, MA Q H, WANG G X . Effect of different postharvest treatments on quality of ‘Abate’ pear during shelf life after cold storage. Chinese Agricultural Science Bulletin, 2015,31(33):273-278. (in Chinese)
[31]	侯玉茹, 李文生, 王宝刚, 苗飞, 石磊 . 1-MCP处理对贮藏后西洋梨货架期品质的影响. 食品工业科技, 2015,36(20):335-338.
	HOU Y R, LI W S, WANG B G, MIAO F, SHI L . Effect of 1-MCP treatment on quality of Pyrus communis L. during shelf-life of storage. Science and Technology of Food Industry, 2015,36(20):335-338. (in Chinese)
[32]	HIWASATANASE K, KINUGASA Y, AMANO S, HASHIMOTO A, NAKANO R, INABA A, KUBO Y . Ethylene is required for both the initiation and progression of softening in pear (Pyrus communis L.) fruit. Journal of Experimental Botany, 2003,54(383):771-779. doi: 10.1093/jxb/erg073 pmid: 12554720
[33]	袁晖, 张利超, 卜海东, 王爱德 . 南果梨成熟相关PG基因筛选及表达分析. 沈阳农业大学学报, 2018,49(5):522-528.
	YUAN H, ZHANG L C, BU H D, WANG A D . The screening and expression analysis of ripening-related PG genes in ‘Nanguo’ pear. Journal of Shenyang Agricultural University, 2018,49(5):522-528. (in Chinese)
[34]	TATEISHI A, MORI H, WATARI J, NAGASHIMA K, YAMAKI S, INOUE H . Isolation, characterization, and cloning of α-L- Arabinofuranosidase expressed during fruit ripening of Japanese pear. Plant Physiology, 2005,138(3):1653-1664. doi: 10.1104/pp.104.056655 pmid: 15965025
[35]	TATEISHI A, INOUE H, SHIBA H, YAMAKI S . Molecular cloning of β-galactosidase from Japanese pear(Pyrus pyrifolia) and its gene expression with fruit ripening. Plant Cell Physiology, 2001,42(5):492-498. doi: 10.1093/pcp/pce059 pmid: 11382815

Related Articles 15

[1]	YUE LiXin, WANG QingHua, WANG ZhenBao, NIMAQIONGJI, LIU ZeZhou, KONG SuPing, ZHANG LiFeng, GAO LiMin. Widely Targeted Metabolomics-Based Analysis of the Differences in Tibetan Bunching Onion and Chive on Nutritional Quality and Flavonoid Metabolites [J]. Scientia Agricultura Sinica, 2026, 59(5): 1070-1086.
[2]	ZHANG LiDong, GUO YiCong, HUANG HongYu, NIE Jing, WANG Bing, LI MengYu, LI JiaWang, SUI XiaoLei, LI YuHe. Correlation Analysis of Cucumber Fruit Quality Integrating Sensory Evaluation with Nutritional Traits and Flavor Compound Characteristics [J]. Scientia Agricultura Sinica, 2026, 59(5): 1087-1100.
[3]	WANG ShaoHua, FAN QiuLi, YANG JinChang, SUN YuJie, YU Niu, JIANG ShouQun. Effects of Different Levels of Mytilaria laosensis Leaves Feeding on Growth Performance, Immune Function, Antioxidant Capacity, Carcass Quality and Meat Quality of Yellow-Feathered Chickens [J]. Scientia Agricultura Sinica, 2026, 59(5): 1111-1127.
[4]	DONG JinLong, ZHAO Ying, YU HaiBing, LÜ JianYe, QIN JiaQi, LIANG Chen, MING Bo, LI ShaoKun. Multi-Model Elucidating of Nutritional Quality Contributions to Maize Kernel Test Weight and Regional Heterogeneity [J]. Scientia Agricultura Sinica, 2026, 59(5): 985-995.
[5]	HAO Kun, CHEN HongDe, ZHANG Wei, ZHONG Yun, DANG MeiRong, ZHU ShiJiang, HUANG ZhiKun, JIN Ying. Comprehensive Evaluation of Water-Nitrogen Management Under Surge-Root Irrigation Based on Citrus Yield, Quality, and Water- Nitrogen Use Efficiency [J]. Scientia Agricultura Sinica, 2026, 59(4): 862-873.
[6]	YAN TingLin, DU YaDan, HU XiaoTao, WANG He, LI XiaoYan, WANG YuMing, NIU WenQuan, GU XiaoBo. The Impacts of Nitrogen Fertilizer Organic Alternatives Under Aerated Drip Irrigation on Cotton Yield and Water Use Efficiency Under Deficit Irrigation Conditions [J]. Scientia Agricultura Sinica, 2026, 59(3): 602-618.
[7]	ZHANG MengBo, TAN HongBing, SHEN Tian, XU MeiLong, ZHOU XinMing, FANG YuLin, JU YanLun. Effects of Different Irrigation Amounts and Anti-transpirant Treatments on Wine Quality [J]. Scientia Agricultura Sinica, 2026, 59(2): 413-426.
[8]	LU Hao, ZHANG MingLong, HAN Mei, YAN QingBiao, LI ZhengPeng, YIN Wen, FAN ZhiLong, HU FaLong, CHAI Qiang. Green Manure Returning via Sheep Digest with Nitrogen Fertilizer Reduction are Beneficial to Improve Wheat Yield and Soil Quality at Qinghai-Tibet Plateau [J]. Scientia Agricultura Sinica, 2026, 59(1): 147-160.
[9]	JI ShengYang, LU BaiYi, LI PeiWu. Some Reflections on Modern Science of Agricultural Product Quality [J]. Scientia Agricultura Sinica, 2025, 58(9): 1830-1844.
[10]	LIU JinSong, WU LongMei, BAO XiaoZhe, LIU ZhiXia, ZHANG Bin, YANG TaoTao. Effects of a Short-Term Reduction in Nitrogen Fertilizer Application Rates on the Grain Yield and Rice Quality of Early and Late-Season Dual-Use Rice in South China [J]. Scientia Agricultura Sinica, 2025, 58(8): 1508-1520.
[11]	WEI WenHua, LI Pan, SHAO GuanGui, FAN ZhiLong, HU FaLong, FAN Hong, HE Wei, CHAI Qiang, YIN Wen, ZHAO LianHao. Response of Silage Maize Yield and Quality to Reduced Irrigation and Combined Organic-Inorganic Fertilizer in Northwest Irrigation Areas [J]. Scientia Agricultura Sinica, 2025, 58(8): 1521-1534.
[12]	XUE YuQi, ZHAO JiYu, SUN WangSheng, REN BaiZhao, ZHAO Bin, LIU Peng, ZHANG JiWang. Effects of Different Nitrogen Forms on Yield and Quality of Summer Maize [J]. Scientia Agricultura Sinica, 2025, 58(8): 1535-1549.
[13]	ZHANG YaFeng, DONG WeiJin, LI QiYun, LU Yang, ZHANG ZhengKun, SUI Li. Effect of Interaction Between Beauveria bassiana and Potassium on Tomato Fruit Quality [J]. Scientia Agricultura Sinica, 2025, 58(6): 1131-1144.
[14]	YANG YongQing, HU PengJu, SONG YaHui, JIN XinXin, SU Qiao, WANG Jin. QTL Mapping of Quality Traits for A Peanut Germplasm SW9721-3 with Ultra-High Oil Content [J]. Scientia Agricultura Sinica, 2025, 58(4): 635-646.
[15]	WU LiDong, LIN ShuTing, QIU YinHui, LIU YaTing, ZHANG Rui, LI YongQing, SHANG Wei, ZHONG LiuQing. Variation of Different Drying Methods on the Quality of Capsicum annuum L. [J]. Scientia Agricultura Sinica, 2025, 58(3): 582-599.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

基因 Gene	登录号 Access No.	正向引物 Forward primer	反向引物 Reverse primer
PG1	KC855751	5′-GCTTATCCTTCATACATGGACACC-3′	5′-CTGATGATGTTGCTATCCCACC-3′
PG2	KC855752	5′-AAATCAGAAAATCCTAAAACCAGC-3′	5′-GCTTATAGCCATCATCAACAACG-3′
ARF1	KC855732	5′-CTTTTTTCCACAATCCTCATTCC-3′	5′-GCTCTCGACGGTATAGAATTTGC-3′
ARF2	KC855733	5′-TAGCAACATTCCCATCCACC-3′	5′-AACATTACACTGCCTATGATCTCG-3′
GAL4	KC855745	5′-GATCAATTCACTATCCCAGAAGC-3′	5′-GTATAACATCCAAGCCTCCAGC-3′
ACT2	GU830958	5′-GGACATTCAACCCCTCGTCT-3′	5’-ATCCTTCTGACCCATACCAACC-3’

处理 Treatment	腐烂率 Decay rate (%)
CK	8.8±2.4c
1-MCP	1.3±2.2d
20℃ 3 d	33.7±0.6b
1-MCP+20℃ 3 d	8.7±2.1c
20℃ 6 d	91.3±5.6a
1-MCP+20℃ 6 d	6.3±2.2cd

Effects of 1-MCP and Delayed Cold-Storage on Quality and Expression of Softening Related Genes in ‘Doyenne du Comice’ Pear During Shelf-Life

RichHTML

PDF