陆地棉DUS测试已知品种DNA指纹数据库构建及应用

doi:10.3864/j.issn.0578-1752.2025.22.002

Abstract

Abstract:

【Objective】DUS (Distinctness, Uniformity, and Stability) testing provides important technical data for cotton variety approval and intellectual property protection, while the selection of similar varieties is a critical step in the process. Based on the revision of “NY/T 2469-2013, Protocol for the identification of cotton variety-SSR marker method”, this study aims further to construct a DNA fingerprint database for known upland cotton varieties for DUS testing in China and determine a genetic similarity threshold for rigorous and precise similar variety selection. 【Method】 Firstly, preliminary screening and subsequent re-screening were conducted on both the SSR primers from NY/T 2469-2013, and newly collected ones to identify a set of primers with stable PCR amplification, high polymorphism and clear peak patterns, replacing the original SSR primer set in the standard. Secondly, the selected SSR primers were employed to genotype the known cotton varieties to construct a DNA fingerprint database for those varieties. Finally, relationships between phenotypic and genetic differences of cotton varieties were investigated and a genetic similarity threshold for similar variety selection was determined. 【Result】 42 pairs of SSR primers covering all 26 chromosomes of upland cotton were selected, including 12 markers from the original standard. Among the selected 42 pairs of primers, 25 pairs amplified two loci, of which 23 revealed only one polymorphic locus, while the remaining two primer pairs (NAU1167 and HAU1413) had both loci showing polymorphism. A total of 164 alleles were detected from the 44 polymorphic loci, with the number of alleles at each locus ranging from 2 to 7. The polymorphism information content (PIC) of the loci varied from 0.15 to 0.66. 2 100 cotton varieties were genotyped using these primers. A DNA fingerprint database of known cotton varieties was constructed with a data integrity of 98.85%. Pairwise comparisons using the DNA data of 648 protected varieties, 843 approved varieties and 2 100 known upland cotton varieties revealed the distribution patterns of genetic differences between varieties among the three categories. More than 90.00% of comparisons in each category were concentrated in the range of 40.00%-70.00%. The proportion of variety pairs with genetic similarity exceeding 80.00% was 0.28%, 0.31% and 0.31% for the protected, approved and known varieties, respectively. Relationship analysis was conducted between phenotypic and genetic differences for 177 upland cotton varieties and their corresponding similar varieties. It was found that for the 54 cotton applications to which similar varieties with genetic similarity exceeding 90.00% existed, 18 (33.33%) failed to meet the distinctness requirement. In contrast, all 123 cotton applications with genetic similarity to their similar varieties below 90.00% could be clearly distinguished, exhibiting clear trait differences, thus fulfilling the distinctness criteria. The genetic similarity threshold for similar variety selection in upland cotton DUS testing based on these SSR markers could be set at 90.00%. For the variety pairs showing genetic similarity above this threshold, further field-based phenotypic evaluations are required. 【Conclusion】 In summary, NY/T 2469-2013, the standard for identifying upland cotton varieties using SSR markers, was revised. A DNA fingerprint database was constructed to support DUS testing of upland cotton in China. Furthermore, a genetic similarity threshold of 90.00% based on these SSR markers was established for selecting similar varieties, enhancing the precision and reliability of this process within DUS testing.

Key words: Upland cotton, DUS testing, SSR marker, similar variety selection, genetic similarity threshold

WANG LiYuan, WANG Hui, WANG MuMu, WANG DongJian, LI RuYu, ZHENG YongSheng, ZHANG Han. Construction and Application of DNA Fingerprint Database for Known Varieties in Upland Cotton DUS Testing[J].Scientia Agricultura Sinica, 2025, 58(22): 4570-4588.

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URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2025.22.002

https://www.chinaagrisci.com/EN/Y2025/V58/I22/4570

Figures/Tables 7

Table 1

Information of 42 pairs of primers used in revised standard"

序号 No.	SSR标记位点 SSR marker	荧光基团 Fluorescence	重复基序 Repeat motif	染色体位置 Chromosome (bp)	产物长度 Size (bp)
1	JNC01	FAM	(AT)9	A01: 110738544-110738773	229
2	JNC02	FAM	(TGCCAA)4	A01: 115886896-115887183 D01: 64518929-64519178	287 249
3	NAU0895	ROX	(ACAT)13	A02: 105363308-105363513 D03: 313063-313252	205 189
4	HAU1413	TAMRA	(GATAGG)4	A03: 4212030-4212314 D03: 49797166-49797432	284 266
5	*NAU1071	FAM	(AGG)5	A03: 103704047-103704204 D02: 65745826-65745988	157 162
6	*NAU1167	ROX	(GATAGG)4	A03: 4212030-4212236 D03: 49797244-49797432	206 188
7	MUCS101	HEX	(GAC)6	A04: 80764224-80764381 D04: 51800594- 51800745	157 151
8	NAU2026	ROX	(GCT)4, (GGA)4	A05: 102020983-102021180 D04: 6171852-6172043	197 191
9	HAU0878	TAMRA	(TCAGGC)5	A05: 15551226-15551358 D05: 14036487-14036613	132 126
10	JNC03	ROX	(AAGCAT)6	A06: 2063861-2064214	353
11	BNL1694	FAM	(ACAT)5	A07: 39612789-39613036 D07: 27649095- 27649318	247 223
12	SHIN-0376	FAM	(TTC)10	A07: 5533136-5533300 D07: 5108398-5108573	164 175
13	BNL3257	FAM	(AC)15, (AT)8	A08: 78637284-78637490	206
14	JNC04	HEX	(AAT)6	A08: 45532050-45532275	225
15	NAU1369	ROX	(AGGCGG)3	A08: 118955689-118955942 D08: 62509921-62510159	253 238
16	*Gh111	TAMRA	(GAA)22	A09: 15754878-15755046	168
17	*DPL0431	TAMRA	(ATA)5	A10: 71856253-71856443	190
18	BNL1231	HEX	(AG)15	A11: 114687920-114688118	198
19	NAU1233	ROX	(TTA)8	A11: 317755-318002 D11: 430059-430297	247 238
20	BNL3261	ROX	(TC)18	A12: 6727325-6727533	208
21	JNC05	TAMRA	(TTC)4, (TCT)14	A12: 84228913-84229159 D12: 45952735- 45952951	246 216
22	*BNL2449	FAM	(TC)16	A13: 34459134-34459278	144
23	JESPR0153	FAM	(GTA)17	A13: 83187366-83187491	125
24	*NAU3468	HEX	(GCA)3	A13: 98049111-98049327 D13: 53711180-53711389	216 209
25	*BNL2646	TAMRA	(AG)17	D01: 41611341-41611483 A01: 71410584-71410680	142 96
26	JNC06	HEX	(TATATG)6	D02: 58293915-58294264	349
27	JNC07	ROX	(TTGTC)5	D03: 88456-88686 A02: 105603420-105603646	230 226
28	*BNL4030	ROX	(GT)8	D04: 6181856-6181967	111
29	CGR6410	FAM	(AGA)13	D04: 6485043-6485194 A05: 101587520-101587651	151 131
30	DPL0071	FAM	(AG)18	D05: 21037170-21037348 A05: 23482746-23482905	178 159
31	*NAU1102	TAMRA	(TCT)10	D05: 57808586-57808826 A04: 6050794-6051013	240 219
32	*DPL0532	ROX	(ATGT)8, (TATG)8	D06: 21142557-21142788 A06: 33746935-33747075	231 140
33	HAU2022	TAMRA	(ATGC)3	D06: 1766468-1766630 A06: 1839082-1839240	162 158
34	JESPR0292	HEX	(GAA)9	D07: 4493445-4493624 A07: 4840237-4840413	179 176
35	*NAU1043	TAMRA	(TTC)12	D07: 5108405-5108641	236
36	*Gh273	FAM	(CT)9	D08: 63045128-63045225	97
37	NAU3100	ROX	(AAG)12	D09: 1513299-1513487 A09: 1560171- 1560374	188 203
38	BNL3948	FAM	(TG)11	D10: 21827957-21828057	100
39	DPL0376	HEX	(AGT)10	D11: 71951682-71951860 A11: 118740400- 118740569	178 169
40	CIR170	HEX	(TG)7	D12: 493264-493421	157
41	JNC08	HEX	(CATCAC)4	D12: 62408314-62408579 A12: 105507504- 105507787	265 283
42	CIR216	TAMRA	(TG)9	D13: 62204584-62204727	143

Table 1

Fig. 1

Table 2

Polymorphism evaluation of 44 SSR primers based on 261 Upland cotton varieties"

序号 No.	SSR标记位点 SSR marker	基因型数量 Genotype No.	等位变异数量 Allele No.	PIC	固定不变位点 Invariable allele	多态性位点等位变异 Major allele (bp)
1	BNL1231	8	5	0.47		188; 190; 194; 196; 198
2	BNL1694	5	4	0.38	222	235; 239; 247; 249
3	BNL2449	6	4	0.39		133; 139; 141; 158
4	BNL2646	7	4	0.37	101	114; 118; 122; 146
5	BNL3261	10	5	0.43		194; 198; 202; 210; 212
6	BNL4030	5	5	0.38		100; 114; 116; 118; 120
7	CIR170	5	4	0.27		155; 157; 159; 161
8	CIR216	4	3	0.37		139; 142; 145
9	DPL0071	9	5	0.56	158	171; 175; 177; 195; 197
10	DPL0431	5	3	0.38		194; 200; 206
11	DPL0532	7	5	0.28	206	211; 231; 235; 239; 243
12	Gh111	13	7	0.64		159; 173; 176; 179; 187; 190; 193
13	Gh273	7	4	0.38		91; 93; 95; 97
14	JESPR0292	5	3	0.39	242	174; 180; 183
15	JESPR153	11	6	0.46		111; 114; 117; 120; 126; 145
16	MUCS101	5	4	0.38	150	0; 156; 162; 177
17	NAU0895	13	5	0.51	188	0; 184; 192; 200; 204
18	NAU1043	8	4	0.46		220; 226; 229; 232
19	NAU1071	5	3	0.39	162	157; 171; 177
20	NAU1102	4	3	0.37	224	233; 239; 242
21	NAU1167-1*	3	2	0.30		190; 196
22	NAU1167-2*	3	2	0.35		202; 208
23	NAU1369	5	4	0.34	247	238; 244; 250; 256
24	NAU2026	3	2	0.36	195	179; 190
25	NAU3468	5	3	0.24	216	220; 226; 232
26	BNL3257	11	5	0.57		192; 204; 206; 208; 210
27	BNL3948	6	3	0.40		93; 97; 99
28	CGR6410	5	3	0.39	129	132; 135; 148
29	DPL0376	5	4	0.34	169	0; 178; 184; 193
30	HAU0878	4	3	0.38	127	110; 116; 134
31	HAU1413-1*	3	2	0.32		269; 275
32	HAU1413-2*	3	2	0.36		281; 287
33	HAU2022	3	2	0.37	161	165; 169
34	NAU1233	12	6	0.66	235	238; 247; 250; 253; 256; 259
35	NAU3100	5	3	0.39	206	184; 187; 190
36	SHIN-0376	8	4	0.47	168	0; 156; 162; 165
37	JNC01	7	4	0.56		219; 222; 226; 228
38	JNC02	3	2	0.37	248	280; 286
39	JNC03	7	4	0.41		340; 352; 358; 368
40	JNC04	6	4	0.15		218; 223; 226; 228
41	JNC05	9	5	0.54	220	232; 235; 247; 253; 256
42	JNC06	6	3	0.39		352; 358; 364
43	JNC07	5	3	0.34	230	232; 237; 242
44	JNC08	4	3	0.37	265	0; 277; 283
平均Mean		6.20	3.73	0.40	/	/

Table 2

Fig. 2

Fig. 3

Table 3

Table 4

Distinctness evaluation of Upland cotton applications in DUS testing from 2022 to 2024"

序号 No.	申请品种 Application variety	近似品种 Similar variety	品种类型 Variety type	遗传相似度 Genetic similarity (%)	测试结果 Testing
1	ZSM7	ZSM4	常规种Conventional varieties	92.05	三性合格Qualified
2	JM9117	JM9116	常规种Conventional varieties	100.00	三性合格Qualified
3	GXM33	GXM32	常规种Conventional varieties	96.51	三性合格Qualified
4	XZ1	EB008	常规种Conventional varieties	93.18	三性合格Qualified
5	ZMS9C05	HM568	常规种Conventional varieties	64.77	三性合格Qualified
6	JCM779	ZMS97	常规种Conventional varieties	73.81	三性合格Qualified
7	JM128	ZMS97	常规种Conventional varieties	67.44	三性合格Qualified
8	KR026	L6269	常规种Conventional varieties	79.27	三性合格Qualified
9	LM247	LM246	常规种Conventional varieties	100.00	三性合格Qualified
10	ZMS95611	YM111	常规种Conventional varieties	71.95	三性合格Qualified
11	YM111	BM15	常规种Conventional varieties	73.17	三性合格Qualified
12	YM409	BM15	常规种Conventional varieties	61.90	三性合格Qualified
13	ZMS9C04	ZMS9D04	常规种Conventional varieties	85.23	三性合格Qualified
14	ZM5022	XFZ010	常规种Conventional varieties	71.59	三性合格Qualified
15	YM616	XFZ010	常规种Conventional varieties	65.48	三性合格Qualified
16	LM4125	XM39	常规种Conventional varieties	72.09	三性合格Qualified
17	YM514	WYM121	常规种Conventional varieties	65.48	三性合格Qualified
18	LM5171	WYM121	常规种Conventional varieties	65.85	三性合格Qualified
19	QCM40	WYM121	常规种Conventional varieties	66.67	三性合格Qualified
20	XJC8	JSHM129	常规种Conventional varieties	84.88	三性合格Qualified
21	ZMZ181	ZMEB001	常规种Conventional varieties	92.05	三性合格Qualified
22	ZMS9710	ZMB905	常规种Conventional varieties	73.81	三性合格Qualified
23	JM127	ZMB905	常规种Conventional varieties	69.05	三性合格Qualified
24	ZMS95613	LM45	常规种Conventional varieties	53.57	三性合格Qualified
25	HM292	XS71143	常规种Conventional varieties	92.86	三性合格Qualified
26	ZM5024	ZMS110	常规种Conventional varieties	70.45	三性合格Qualified
27	ZH8142R	HM12	常规种Conventional varieties	54.55	三性合格Qualified
28	ZMS1601	JK1062	常规种Conventional varieties	79.07	三性合格Qualified
29	H46R	LZM169	常规种Conventional varieties	81.82	三性合格Qualified
30	LM261	LMY36	常规种Conventional varieties	82.14	三性合格Qualified
31	YM516	XM8	常规种Conventional varieties	55.81	三性合格Qualified
32	XM8	JFY122	常规种Conventional varieties	88.64	三性合格Qualified
33	ZMS99020	ZM001	杂交种Hybrid varieties	72.09	三性合格Qualified
34	HZM15	YHM19	杂交种Hybrid varieties	61.63	三性合格Qualified
35	ZMS1603	ZMS1701	杂交种Hybrid varieties	79.76	三性合格Qualified
36	HZM9	HZM14	杂交种Hybrid varieties	82.56	三性合格Qualified
37	HZM15	HZM13	杂交种Hybrid varieties	87.21	三性合格Qualified
38	JZ277	ZMS95614	杂交种Hybrid varieties	69.32	三性合格Qualified
39	ZMS95614	GXM30	杂交种Hybrid varieties	74.42	三性合格Qualified
40	ZMS9B04	ZMS9B05	杂交种Hybrid varieties	75.00	三性合格Qualified
41	LH462	LZ311	杂交种Hybrid varieties	55.00	三性合格Qualified
42	ZMS9D05	SNSF17	杂交种Hybrid varieties	78.57	三性合格Qualified
43	ZZM28	XK18	杂交种Hybrid varieties	58.75	三性合格Qualified
44	NX1	EB008	常规种Conventional varieties	92.05	特异性不合格Non-distinct
45	JZ658	X822	常规种Conventional varieties	97.73	三性合格Qualified
46	JS83	CM45	常规种Conventional varieties	81.40	三性合格Qualified
47	HM883	HM802	常规种Conventional varieties	87.21	三性合格Qualified
48	XM3	XM4	常规种Conventional varieties	86.05	三性合格Qualified
49	LM45	JS813	常规种Conventional varieties	60.23	三性合格Qualified
50	JS813	JM623	常规种Conventional varieties	79.55	三性合格Qualified
51	HM872	YF39	常规种Conventional varieties	84.88	三性合格Qualified
52	HM5104	HM3026	常规种Conventional varieties	69.05	三性合格Qualified
53	HM5201	HM3026	常规种Conventional varieties	97.62	三性合格Qualified
54	JND46	J3816	常规种Conventional varieties	70.45	三性合格Qualified
55	HM5272	HM12	常规种Conventional varieties	65.91	三性合格Qualified
56	LM43	HM121	常规种Conventional varieties	70.24	三性合格Qualified
57	HW339	LZM169	常规种Conventional varieties	75.00	三性合格Qualified
58	H6203A	JF106	常规种Conventional varieties	71.59	三性合格Qualified
59	HM5123	ZMS41	常规种Conventional varieties	77.91	三性合格Qualified
60	ZM5014	ZMEB002	杂交种Hybrid varieties	97.73	三性合格Qualified
61	LZ206	JZ299	杂交种Hybrid varieties	51.14	三性合格Qualified
62	ZMS1812	ZMS1702	杂交种Hybrid varieties	97.73	特异性不合格Non-distinct
63	ZMS1802	ZMS9702	杂交种Hybrid varieties	96.59	三性合格Qualified
64	ZMS1811	JZ277	杂交种Hybrid varieties	71.59	三性合格Qualified
65	DM23	HM6105	常规种Conventional varieties	76.19	三性合格Qualified
66	P1528	MH410433	常规种Conventional varieties	81.82	三性合格Qualified
67	ZSM31	HM18	常规种Conventional varieties	55.81	三性合格Qualified
68	LM481	ZMS9A05	常规种Conventional varieties	63.64	三性合格Qualified
69	SNM22	H613	常规种Conventional varieties	68.60	三性合格Qualified
70	LM2436	LM696	常规种Conventional varieties	77.38	三性合格Qualified
71	FM20	WYM121	常规种Conventional varieties	88.10	三性合格Qualified
72	LM264	LH462	常规种Conventional varieties	100.00	三性合格Qualified
73	LM306	LM395	常规种Conventional varieties	60.47	三性合格Qualified
74	LM482	LM265	常规种Conventional varieties	59.30	三性合格Qualified
75	JND48	HR5105	常规种Conventional varieties	51.16	三性合格Qualified
76	ZMS1602	XM8	常规种Conventional varieties	58.14	三性合格Qualified
77	JK1651	ZSM8	常规种Conventional varieties	100.00	三性合格Qualified
78	GXM36	JZ716	杂交种Hybrid varieties	78.41	三性合格Qualified
79	HZM17	YH172	杂交种Hybrid varieties	73.86	三性合格Qualified
80	HM11	HZM9	杂交种Hybrid varieties	90.91	三性合格Qualified
81	JFZ17	Z5009	杂交种Hybrid varieties	70.45	三性合格Qualified
82	ZMS100913	ZMS100901	杂交种Hybrid varieties	76.14	三性合格Qualified
83	ZM5026	ZMEB002	杂交种Hybrid varieties	94.19	特异性不合格Non-distinct
84	ZMS9E01	ZMS9D05	杂交种Hybrid varieties	75.58	三性合格Qualified
85	ZMS96021	ZMS9E01	杂交种Hybrid varieties	64.77	三性合格Qualified
86	LWM3	YM3	常规种Conventional varieties	81.82	三性合格Qualified
87	DM13	JS868	常规种Conventional varieties	64.10	三性合格Qualified
88	HM12	JS868	常规种Conventional varieties	68.18	三性合格Qualified
89	HM3134	LM696	常规种Conventional varieties	65.85	三性合格Qualified
90	BM17	BM17	常规种Conventional varieties	98.86	特异性不合格Non-distinct
91	ZB79185R	ZMS9706	常规种Conventional varieties	100.00	特异性不合格Non-distinct
92	ZMS100915	ZMS9A07	常规种Conventional varieties	72.50	三性合格Qualified
93	ZJMT01	HD109	常规种Conventional varieties	97.37	三性合格Qualified
94	ZMS96024	JS835	常规种Conventional varieties	57.95	三性合格Qualified
95	YM706	J172	常规种Conventional varieties	53.41	三性合格Qualified
96	LWM8	XLZ61	常规种Conventional varieties	88.37	三性合格Qualified
97	LWM1	XLZ61	常规种Conventional varieties	54.65	三性合格Qualified
98	JDHM618	KSM8	常规种Conventional varieties	100.00	特异性不合格Non-distinct
99	ZMS95612	ZMS110	常规种Conventional varieties	91.86	三性合格Qualified
100	ZSM13	ZSM8	常规种Conventional varieties	98.86	三性合格Qualified
101	ZMS100903	LM1127	常规种Conventional varieties	100.00	三性合格Qualified
102	H963	JM35	常规种Conventional varieties	97.73	特异性不合格Non-distinct
103	HM6319	HM3026	常规种Conventional varieties	97.73	特异性不合格Non-distinct
104	HM554	SZ1	常规种Conventional varieties	98.84	三性合格Qualified
105	HM922	HM568	常规种Conventional varieties	98.86	特异性不合格Non-distinct
106	JF01	J172	常规种Conventional varieties	96.51	特异性不合格Non-distinct
107	SNMTC15	YXM28	常规种Conventional varieties	97.73	三性合格Qualified
108	J3010	XM62	常规种Conventional varieties	100.00	三性合格Qualified
109	GXM53	YM10	常规种Conventional varieties	97.73	三性合格Qualified
110	GXM52	CM531	常规种Conventional varieties	97.73	三性合格Qualified
111	ZMS2312	ZMS2316	常规种Conventional varieties	98.86	特异性不合格Non-distinct
112	ZMS2310	ZMS1607	常规种Conventional varieties	86.36	三性合格Qualified
113	JM628	ZSM22	常规种Conventional varieties	97.73	特异性不合格Non-distinct
114	TLJ168	SFK218	常规种Conventional varieties	97.73	特异性不合格Non-distinct
115	ZMS2313	ZMS1702	杂交种Hybrid varieties	80.68	三性合格Qualified
116	ZMS2314	ZMS2306	杂交种Hybrid varieties	98.86	三性合格Qualified
117	ZMS2311	ZMS1802	杂交种Hybrid varieties	86.36	三性合格Qualified
118	ZA057	JS367	常规种Conventional varieties	90.91	三性合格Qualified
119	HM216	ZM9978	常规种Conventional varieties	65.91	三性合格Qualified
120	YM603	ZM9978	常规种Conventional varieties	64.77	三性合格Qualified
121	HM256	SNM11	常规种Conventional varieties	88.64	三性合格Qualified
122	XHM8	HM1091	常规种Conventional varieties	98.86	特异性不合格Non-distinct
123	ZMS9C07	HM288	常规种Conventional varieties	70.93	三性合格Qualified
124	HXM501	JK1651	常规种Conventional varieties	63.64	三性合格Qualified
125	ZM316	ZM425	常规种Conventional varieties	92.05	特异性不合格Non-distinct
126	JF178	MH410433	常规种Conventional varieties	90.91	三性合格Qualified
127	JF238	LM338	常规种Conventional varieties	93.18	特异性不合格Non-distinct
128	LM351	LM552	常规种Conventional varieties	90.91	三性合格Qualified
129	HXM9	JM918	常规种Conventional varieties	100.00	三性合格Qualified
130	J3036	J3058	常规种Conventional varieties	77.27	三性合格Qualified
131	J3076	J3058	常规种Conventional varieties	75.00	三性合格Qualified
132	ZR055	JS367	常规种Conventional varieties	90.91	三性合格Qualified
133	HM118	ZMS95622	常规种Conventional varieties	63.64	三性合格Qualified
134	GXM51	ZR718	常规种Conventional varieties	77.27	三性合格Qualified
135	ZR113	ZA109	常规种Conventional varieties	98.86	三性合格Qualified
136	LM249	HM5278	常规种Conventional varieties	73.86	三性合格Qualified
137	HZ28	ZMS1607	杂交种Hybrid varieties	60.23	三性合格Qualified
138	J3018	JND23	常规种Hybrid varieties	86.36	三性合格Qualified
139	JND47	JZ716	杂交种Hybrid varieties	70.45	三性合格Qualified
140	HZ69	JZ716	杂交种Hybrid varieties	80.68	三性合格Qualified
141	LZ316	ZMS9A03	杂交种Hybrid varieties	62.50	三性合格Qualified
142	ZMS9A03	ZMS9B08	杂交种Hybrid varieties	64.77	三性合格Qualified
143	ZMS9B08	ZMS95623	杂交种Hybrid varieties	65.91	三性合格Qualified
144	ZMS9B07	ZM9134	杂交种Hybrid varieties	64.77	三性合格Qualified
145	LM272	LM271	常规种Conventional varieties	84.09	三性合格Qualified
146	LM49	ZM328	常规种Conventional varieties	68.18	三性合格Qualified
147	JF108	ZM9003	常规种Conventional varieties	65.91	三性合格Qualified
148	HXM50	ZMS99033	杂交种Hybrid varieties	60.23	三性合格Qualified
149	JND44	HM216	常规种Conventional varieties	52.27	三性合格Qualified
150	JS18	ZMSEM1704	常规种Conventional varieties	78.41	三性合格Qualified
151	ZMZ182	ZM425	常规种Conventional varieties	85.23	三性合格Qualified
152	HMM36	JN16	常规种Conventional varieties	95.45	三性合格Qualified
153	HMM35	JX2103	常规种Conventional varieties	100.00	特异性不合格Non-distinct
154	LWM9	HM16	常规种Conventional varieties	72.73	三性合格Qualified
155	LWM10	HM6105	常规种Conventional varieties	60.23	三性合格Qualified
156	LM22	LM23	常规种Conventional varieties	89.77	三性合格Qualified
157	LWM11	SDM2	常规种Conventional varieties	84.09	三性合格Qualified
158	LWM15	ZSM37	常规种Conventional varieties	84.09	三性合格Qualified
159	LWM13	SDM21	常规种Conventional varieties	97.73	特异性不合格Non-distinct
160	JM906	J3018	常规种Conventional varieties	65.91	三性合格Qualified
161	JND33	JND45	常规种Conventional varieties	80.23	三性合格Qualified
162	XM4	XM3	常规种Conventional varieties	86.05	三性合格Qualified
163	ZMS9D04	ZMS9C04	常规种Conventional varieties	85.23	三性合格Qualified
164	LM266	LM258	常规种Conventional varieties	98.86	三性合格Qualified
165	JS370	JN16	常规种Conventional varieties	86.36	三性合格Qualified
166	DM14	JM1109	常规种Conventional varieties	80.68	三性合格Qualified
167	ZSM47	SDM192	常规种Conventional varieties	100.00	三性合格Qualified
168	JM1109	DM14	常规种Conventional varieties	80.68	三性合格Qualified
169	H505	GXM37	常规种Conventional varieties	80.68	三性合格Qualified
170	GXM37	H505	常规种Conventional varieties	80.68	三性合格Qualified
171	ZMEB002	ZM5014	杂交种Hybrid varieties	97.73	三性合格Qualified
172	JF188	JF272	常规种Conventional varieties	81.40	三性合格Qualified
173	JF272	JF188	常规种Conventional varieties	81.40	三性合格Qualified
174	JND45	JDN33	常规种Conventional varieties	80.23	三性合格Qualified
175	ZSM16	ZSM16	常规种Conventional varieties	95.24	三性合格Qualified
176	GK99-1	GXM25	常规种Conventional varieties	84.09	三性合格Qualified
177	X98	JM3345	常规种Conventional varieties	100.00	三性合格Qualified

Table 4

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遗传相似度 Genetic similarity (%)	2100份已知品种两两比较 Pairwise comparison of 2100 cotton varieties			648份品种权授权品种两两比较 Pairwise comparison of 648 protected cotton varieties		843份审定品种两两比较 Pairwise comparison of 843 approved cotton varieties
遗传相似度 Genetic similarity (%)	涉及成对比较的对数 The number of pairwise comparisons	成对比较的百分比 Percentage of pairwise comparisons (%)	成对比较的累计百分比 Cumulative percentage of pairwise comparisons (%)	涉及成对比较的对数 The number of pairwise comparisons	成对比较的百分比 Percentage of pairwise comparisons (%)	涉及成对比较的对数 The number of pairwise comparisons	成对比较的百分比 Percentage of pairwise comparisons (%)
GS=100.00	201	0.01	0.01	9	0.00	30	0.01
95.00<GS<100.00	1216	0.06	0.06	67	0.03	150	0.04
90.00<GS<=95.00	1236	0.06	0.12	60	0.03	150	0.04
80.00<GS<=90.00	4141	0.19	0.31	456	0.22	758	0.21
70.00<GS<=80.00	39628	1.80	2.11	4414	2.11	7388	2.08
60.00<GS<=70.00	347729	15.78	17.88	39044	18.63	63344	17.85
50.00<GS<=60.00	878750	39.87	57.76	89500	42.69	149513	42.13
40.00<GS<=50.00	772602	35.06	92.81	65449	31.22	114052	32.14
30.00<GS<=40.00	151733	6.88	99.70	10273	4.90	18836	5.31
20.00<GS<=30.00	6674	0.30	100.00	355	0.17	680	0.19
10.00<GS<=20.00	40	0.00	100.00	1	0.00	2	0.00
5.00<GS<=10.00	0	0.00	100.00	0	0.00	0	0.00
0.00<=GS<=5.00	0	0.00	100.00	0	0.00	0	0.00

Construction and Application of DNA Fingerprint Database for Known Varieties in Upland Cotton DUS Testing

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