Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (18): 3841-3848.doi: 10.3864/j.issn.0578-1752.2012.18.017

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Effects of S-Adenosylmethionine (SAM) on Adipogenic Differentiation and Lipid Accumulation of C2C12 Cells

 YU  Bo-Yang, 吕Wen-Tao , JU  Ting-Ting, LIU  Yang, JIANG  Mei-Hua, YIN  Jing-Dong   

  1. 1.中国农业大学动物科技学院/动物营养学国家重点实验室，北京 100193
  • Received:2012-02-15 Online:2012-09-15 Published:2012-05-10

PDF

753

Cited

3

Abstract: 【Objective】This experiment was conducted to investigate the effects of S-adenosylmethionine (SAM) on adipogenesis and lipogenesis of C2C12 mouse myoblast. 【Method】Cells were cultured in high glucose DMEM medium containing 0 mmol•L-1, 0.25 mmol•L-1, 1.0 mmol•L-1, and 2.0 mmol•L-1 SAM. After treated for 0 h, 24 h, 36 h, and 48 h, cells were stained with Red Oil O and observed under microscope. The lipid accumulation in cytosol was determined by optical density (OD) measurement. After being treated for 2 d, cells were harvested for collection of total RNA and protein. Then RT-PCR and western blotting approaches were used to determine mRNA and protein expression levels of several genes related with adipogenesis and lipogenesis. 【Result】The results showed that, compared with the control group, C2C12 cells presented typical features of adipocytes when observed under microscope after treated with SAM. SAM also increased the number of lipid droplets in the cells. In addition, the increasing trend became more significant when cells were treated with higher concentration of SAM. At the same time, mRNA and protein expression levels of key regulator of differentiation of adipocytes, PPARγ and C/EBPα, were elevated (P＜0.05) after treatment. mRNA expression levels of other lipogenesis related genes, such as aP2, FAS and SREBP-1, were also elevated markedly. Compared with the control group, the relative mRNA levels of these three genes were increased by 6.86 (P＜0.01), 3.45 (P＜0.05), and 3.48 (P＜0.01) folds, respectively, in 2.0 mmol•L-1 group. 【Conclusion】The results indicated that SAM had the ability to promote adipogenic differentiation and lipid accumulation of C2C12 cells.

Key words: mouse, skeletal muscle fibroblast, SAM, adipogenic differentiation, lipid accumulation

[1]Wood J D, Enser M, Fisher A V, Nute G R, Richardson R I, Sheard P R. Manipulating meat quality and composition. Proceeding of the Nutrition Society, 1999, 58(2): 363-370.

[2]van Laack R L, Stevens S G, Stalder K J. The influence of ultimate pH and intramuscular fat content on pork tenderness and tenderization. Journal of Animal Science, 2002, 79(2): 392-397.

[3]Suzuki K, Irie M, Kadowaki H, Shibata T, Kumagai M, Nishida A. Genetic parameter estimates of meat quality traits in Duroc pigs selected for average daily gain, longissimus muscle area, backfat thickness, and intramuscular fat content. Journal of Animal Science, 2005, 83(9): 2058-2065.

[4]Knapp P, Alfons W, Johann S. Genetic parameters for lean meat content and meat quality traits in different pig breed. Livestock Production Science, 1997, 52(1): 69-73.

[5]Fernandez X, Monin G, Talmant A. Influence of intramuscular fat content on the quality of pig meat-1. Composition of the lipid fraction and sensory characteristics of m. longissimus lumborum. Meat Science, 1999, 53(1): 59-65.

[6]Cristancho A G, Lazar M A. Forming functional fat: a growing understanding of adipocyte differentiation. Nature Reviews Molecular Cell Biology, 2011, 12: 722-734.

[7]Mayoral A I, Dorado M, Guillen M T. Development of meat and carcass quality characteristics in Iberian pigs reaed outdoors. Meat Science, 1999, 52(3): 315-324.

[8]Joe A W, Yi L, Even Y, Vogl A W, Rossi F M. Depot-specific differences in adipogenic progenitor abundance and proliferative response to high-fat diet. Stem Cells, 2009, 27(10): 2563-2570.

[9]Gesta S, Tseng Y H, Kahn C R. Developmental origin of fat: tracking obesity to its source. Cell, 2007, 131(2): 242-256.

[10]Seale P, Bjork B, Yang W L, Kajimura S, Chin S, Kuang S, Scime A, Devarakonda S, Conroe M H, Bromage H E, Tempst P, Rudnick M A, Beier D R, Spiegelman B M. PRDM16 controls a brown fat/skeletal muscle switch. Nature, 2008, 454: 961-967.

[11]Aguiari P S, Leo S, Zavan B, Vindigni V, Rimessi A, Bianchi K, Franzin C, Cortivo R, Rossato M, Vettor  R, Abatangelo G, Pozzan T, Pinton P, Rizzuto R. High glucose induce adipogenic differentiation of muscle-derived stem cells. Proceeding of the National Academy of Science of the United States of America, 2008, 105(4): 1226-1231. 

[12]Yue T, Fang Q, Yin J D, Li D F, Li W. S-adenosylmethionine stimulates fatty acid metabolism-linked gene expression in porcine muscle satellite cells. Molecular Biology Reports, 2010, 37: 3143-3149.

[13]Teboul L, Gaillard D, Staccini L, Inadera H, Amri E Z, Grimaldi P A. Thiazolidinediones and fatty acids convert myogenic cells into adipose-like cells. The Journal of Biological Chemistry, 1995, 270(47): 28183-28187.

[14]Fujita T, Furukawa S, Morita K, Ishihara T. Glucosamine induces lipid accumulation and adipogenic change in C2C12 myoblasts. Biochemical and Biophysical Research Communications, 2005, 328(2): 369-374.

[15]Lee S J, Lee E J, Kim S H, Choi I. IL-17A promotes transdifferentiation of mouse myoblast cells (C2C12) into adipocytes by increasing the expression of peroxisome proliferator-activated receptor γ through CAAT/enhancer binding protein β signaling. Biotechnology Letters, 2011, 33(2): 29-235.

[16]Itoigawa Y, Kishimoto K N, Okuno H. Hypoxia induces adipogenic differentitation of myoblastic cell lines. Biochemical and Biophysical Research Communications, 2010, 399(4): 721-726.

[17]Hong Y H, Nishimura Y, Hishikawa D, Tsuzuli H, Miyahara H, Gotoh C, Choi K C, Feng D D, Chen C, Lee H G, Kazuo K, Roh S G, Sasaki S. Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43. Endocrinology, 2006, 146(12): 5092-5099.

[18]Mie M H, Ohgushi H, Yanagida Y, Haruyama T, Kobatake E, Aizawa M. Osteogenesis coordinated in C3H10T1/2 cells by adipogenesis- dependent BMP-2 expression system. Tissue Engineering, 2000, 6(1): 9-18.

[19]Pollard J W, Walker J M. Methods in Molecular Biology. Totowa, NJ: Humana Press, 2011: 197-207.

[20]Farmer S R. Transcriptional control of adipocyte formation. Cell Metabolism, 2006, 4(4): 263-273.

[21]Tontonoz P, Hu E, Spiegelman B M. Stimulation of adipogenesis in fibroblasts by PPARγ2, a lipid activated transcription factor. Cell, 79(7): 1147-1156.

[22]Linhart H G, Ishimura K, DeMayo F, Kibe T, Repka D, Poindexter B, Bick R J, Darlington G J. C/EBPalpha is required for differentiation of white but not brown adipose tissue. Proceeding of the National Academy of Science of the United States of America, 2001, 98(22): 12532-12537.

[23]Wu Z, Rosen E D, Brun R, Hauser S, Adelmant G, Troy A E, MaKeon C, Darlington G J, Spiegelman B M. Cross-regulation of C/EBPαand PPARγ controls the transcriptional pathway of adipogenesis and insulin sensitivity. Molecular Cell, 1999, 3(2): 151-158.

[24]Badger A M, Newman T M, Satterfield J L, Semenkovich C F. Regulation of fatty acid synthase (FAS). Progress in Lipid Research, 1997, 36: 45-53.

[25]Kim J B, Spiegelman B M. ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism. Genes and Development, 1996, 10(9): 1096-1107.

[26]Ericsson J, Jackson S M, Kim J B, Spiegelman B M, Edwards P A. Identification of glycerol-3-phosphate acyl transferase as an adipocyte determination and differentiation factor 1 and sterol regulatory element-binding protein-responsive gene. The Journal of Biological Chemistry, 1997, 272(11): 7298-7305.

[27]Cantoni G L. Activation of methionine for transmethylation. The Journal of Biological Chemistry, 1951, 189(2): 745-754.

[28]Cantoni G L. S-adenosylmethionine: a new intermediate formed enzymatically from L-methionine and adenosinetriphosphase. The Journal of Biological Chemistry, 1953, 204(1): 403-416.

[29]Herrmann W, Schorr H, Obeid R, Makowski J, Fowler B, Kuhlmann M K. Disturbed homocysteine and methionine cycle intermediates S-adenosylhomocysteine and S-adenosylmethionine are related to degree of renal insufficiency in type 2 diabetes. Clinical Chemistry, 2005, 51(5): 891-897.

[30]Philips D I, Caddy S, Ilic V, Fielding B A, Frayn K N, Borthwick A C, Taylor R. Intramuscular triglyceride and muscle insulin sensitivity: evidence for a relationship in nondiabetic subjects. Metabolism, 1996, 45(8): 947-950.

[31]Koyama K, Chen G, Lee Y, Unger R H. Tissue triglycerides, insulin resistance, and insulin production: implications for hyperinsulinemia of obesity. The American Journal of Physiology, 1997, 273(4): E708-E713.

[32]Dobbins R L, Szczepaniak L S, Bentley B, Esser V, Myhill J, McGarry J D. Prolonged inhibition of muscle carnitine palmitoyltransferase-1 promotes intramyocellular lipid accumulation and insulin resistance in rats. Diabetes, 2001, 50(1): 123-130.

[33]Mato J M, Alvarez L, Ortiz P, Pajares MA. S-adenosylmethionine synthesis: molecular mechanisms and clinical implications. Pharmacology and Therapeutics, 1997, 73(3): 265-280.

[34]Giulidori P, Galli-Kienle M, Catto E, Stramentinoli G. Transmethylation, transsulfuration, and aminopropylation reactions of S-adenosyl-L- methionine in vivo. The Journal of Biological Chemistry, 1984, 259(7): 4205-4211.

[35]Cho Y W, Hong S H, Jin Q H, Wang L F, Lee J E, Gavrilova O, Ge K. Histone methylation regulator PTIP is required for PPARγ and C/EBPα expression and adipogenesis. Cell Metabolism, 2009, 10(1): 27-39.

[36]Wang L F, Jin Q H, Lee J E, Su I, Ge K. Histone H3K27 methyltransferase Ezh2 represses Wnt genes to facilitate adipogenesis. Proceeding of the National Academy of Science of the United States of America, 2010, 107(16): 7317-7322.
[1] ZHAO QingYao, WANG XiaoMing, XING Tong, LI LingYun, XU XingLian, ZHAO Xue. Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Intestinal Polysaccharides from Yellow-Feathered Chickens [J]. Scientia Agricultura Sinica, 2026, 59(6): 1317-1332.
[2] LI MingLi, WEN CaiYun, MA DongHao, LI CunJun, WANG YuWen, KANG Lu, LU Miao. Construction of Salinity Prediction Model Based on Optimal Selection of Soil Hyperspectral Characteristic Bands [J]. Scientia Agricultura Sinica, 2025, 58(20): 4054-4069.
[3] LUO ZiShu, ZHANG YiJia, ZHOU Rong, ZHANG YanXin, ZHOU Ting, YOU Jun, WANG LinHai. Characterization of Antioxidant Activities in Sesame Leaves and Screening of High Antioxidant Germplasm [J]. Scientia Agricultura Sinica, 2025, 58(19): 3814-3824.
[4] QI XiangYu, LI XinRu, CHEN ShuangShuang, FENG Jing, CHEN HuiJie, LIU XinTong, JIN YuYan, DENG YanMing. Identification of the FLA Gene Family and Functional Analysis of JsFLA2 in Jasminum sambac [J]. Scientia Agricultura Sinica, 2025, 58(17): 3516-3530.
[5] LÜ JinLing, YOU Ke, WANG XiaoFei, XIAO Qiang, LI WenFeng, MA Jin, YANG Qing, ZHANG JinPing, KONG HaiJiang, CHANG YunHua. Variation Characteristics and Key Influencing Factors of Near-Surface Ambient Ammonia Concentration in Typical Cropland Areas in Henan Province [J]. Scientia Agricultura Sinica, 2025, 58(1): 127-140.
[6] WU YuZhen, HUANG LongYu, ZHOU DaYun, HUANG YiWen, FU ShouYang, PENG Jun, KUANG Meng. Construction of SSR Fingerprint Library and Comprehensive Evaluation for Approved Cotton Varieties in China [J]. Scientia Agricultura Sinica, 2024, 57(8): 1430-1443.
[7] SU XiaoYu, TAN ZhengWei, LI ChunMing, LI Lei, LU DanDan, YU YongLiang, DONG Wei, AN SuFang, YANG Qing, SUN Yao, XU LanJie, YANG HongQi, LIANG HuiZhen. Analysis of Genome-Wide Methylation Differences and Associated Gene Expression of Sesame Varieties Under High Temperature Stress [J]. Scientia Agricultura Sinica, 2024, 57(24): 4825-4838.
[8] RONG YaSi, LI Feng, ZHANG PengYu, WANG DongYong, SU XiaoYu, TIAN Yuan, GAO TongMei. Evaluation of High Temperature Tolerance and Selection of Sesame (Sesamum indicum L.) Cultivars at Full Flowering Stage Based on Principal Components-Cluster Analysis [J]. Scientia Agricultura Sinica, 2024, 57(20): 3957-3973.
[9] YANG Xi, YOU Jun, ZHOU Rong, FANG Sheng, ZHANG YanXin, WU ZiMing, WANG LinHai. Establishment of High-Throughput Detection Method for Phytic Acid Content in Sesame Seeds and Screening of Low Phytic Acid Germplasms [J]. Scientia Agricultura Sinica, 2024, 57(12): 2282-2294.
[10] LAI ChunWang, ZHOU XiaoJuan, CHEN Yan, LIU MengYu, XUE XiaoDong, XIAO XueChen, LIN WenZhong, LAI ZhongXiong, LIN YuLing. Identification of Ethylene Synthesis Pathway Genes in Longan and Its Response to ACC Treatment [J]. Scientia Agricultura Sinica, 2022, 55(3): 558-574.
[11] JU Ming, MIAO HongMei, HUANG YingYing, MA Qin, WANG HuiLi, WANG CuiYing, DUAN YingHui, HAN XiuHua, ZHANG HaiYang. Analysis of Cross Compatibility Variation Among Diverse Sesamum Species and Biological Characteristics of the Interspecific Hybrid Progenies [J]. Scientia Agricultura Sinica, 2022, 55(20): 3897-3909.
[12] WU Jun,GUO DaQian,LI Guo,GUO Xi,ZHONG Liang,ZHU Qing,GUO JiaXin,YE YingCong. Prediction of Soil Organic Carbon Content in Jiangxi Province by Vis-NIR Spectroscopy Based on the CARS-BPNN Model [J]. Scientia Agricultura Sinica, 2022, 55(19): 3738-3750.
[13] WeiLi ZHANG,H KOLBE,RenLian ZHANG,DingXiang ZHANG,ZhanGuo BAI,Jing ZHANG,HuaDing SHI. Overview of Soil Survey Works in Main Countries of World [J]. Scientia Agricultura Sinica, 2022, 55(18): 3565-3583.
[14] GUO Can,YUE XiaoFeng,BAI YiZhen,ZHANG LiangXiao,ZHANG Qi,LI PeiWu. Research on the Application of a Balanced Sampling-Random Forest Early Warning Model for Aflatoxin Risk in Peanut [J]. Scientia Agricultura Sinica, 2022, 55(17): 3426-3436.
[15] ZHONG YanPing,SHI LiSong,ZHOU Rong,GAO Yuan,HE YanQing,FANG Sheng,ZHANG XiuRong,WANG LinHai,WU ZiMing,ZHANG YanXin. Establishment of High Efficient Extraction and Detection Technology of Sesamin and Screening of High Sesamin Germplasm [J]. Scientia Agricultura Sinica, 2022, 55(11): 2109-2120.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd