Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (18): 3841-3848.doi: 10.3864/j.issn.0578-1752.2012.18.017

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Effects of S-Adenosylmethionine (SAM) on Adipogenic Differentiation and Lipid Accumulation of C2C12 Cells

 YU  Bo-Yang, 吕Wen-Tao , JU  Ting-Ting, LIU  Yang, JIANG  Mei-Hua, YIN  Jing-Dong   

  1. 1.中国农业大学动物科技学院/动物营养学国家重点实验室，北京 100193
  • Received:2012-02-15 Online:2012-09-15 Published:2012-05-10

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Abstract: 【Objective】This experiment was conducted to investigate the effects of S-adenosylmethionine (SAM) on adipogenesis and lipogenesis of C2C12 mouse myoblast. 【Method】Cells were cultured in high glucose DMEM medium containing 0 mmol•L-1, 0.25 mmol•L-1, 1.0 mmol•L-1, and 2.0 mmol•L-1 SAM. After treated for 0 h, 24 h, 36 h, and 48 h, cells were stained with Red Oil O and observed under microscope. The lipid accumulation in cytosol was determined by optical density (OD) measurement. After being treated for 2 d, cells were harvested for collection of total RNA and protein. Then RT-PCR and western blotting approaches were used to determine mRNA and protein expression levels of several genes related with adipogenesis and lipogenesis. 【Result】The results showed that, compared with the control group, C2C12 cells presented typical features of adipocytes when observed under microscope after treated with SAM. SAM also increased the number of lipid droplets in the cells. In addition, the increasing trend became more significant when cells were treated with higher concentration of SAM. At the same time, mRNA and protein expression levels of key regulator of differentiation of adipocytes, PPARγ and C/EBPα, were elevated (P＜0.05) after treatment. mRNA expression levels of other lipogenesis related genes, such as aP2, FAS and SREBP-1, were also elevated markedly. Compared with the control group, the relative mRNA levels of these three genes were increased by 6.86 (P＜0.01), 3.45 (P＜0.05), and 3.48 (P＜0.01) folds, respectively, in 2.0 mmol•L-1 group. 【Conclusion】The results indicated that SAM had the ability to promote adipogenic differentiation and lipid accumulation of C2C12 cells.

Key words: mouse, skeletal muscle fibroblast, SAM, adipogenic differentiation, lipid accumulation

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