Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (6): 1127-1138.doi: 10.3864/j.issn.0578-1752.2022.06.006

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Prediction of Maize Yield in Relay Strip Intercropping Under Different Water and Nitrogen Conditions Based on PLS

TAN XianMing(),ZHANG JiaWei,WANG ZhongLin,CHEN JunXu,YANG Feng(),YANG WenYu   

  1. College of Agronomy, Sichuan Agricultural University/Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture/Sichuan Engineering Research Center for Crop Strip Intercropping System, Chengdu 611130
  • Received:2021-05-17 Accepted:2021-10-08 Online:2022-03-16 Published:2022-03-25
  • Contact: Feng YANG E-mail:2019301094@stu.sicau.edu.cn;f.yang@sicau.edu.cn

Abstract:

【Objective】 This study was designed mainly to provide technical means for non-destructive prediction of intercropped maize yield. The prime objective of our study was to construct a “hyperspectral parameter-photosynthetic pigment-yield” model from the hyperspectral data. 【Method】 Based on field trials of different years, locations, varieties, and treatments (nitrogen fertilizer, moisture), the relationships among photosynthetic pigment parameters, canopy hyperspectral parameters, and maize yield at each growth period and the entire growth period of intercropping maize were comprehensively analyzed. In addition, the optimal growth period and photosynthetic pigment parameters for maize yield prediction were also clarified. Then, the prediction model of yield was constructed based on linear function, quadratic function and partial least squares regression (PLS). 【Result】 Among the photosynthetic pigment-yield prediction models, the PLS prediction model of yield based on canopy carotenoid density had the best effect (R 2=0.882, RMSE=0.669 t·hm -2). In the spectral parameter-photosynthetic pigment analysis, the chlorophyll content during the tasseling stage had the best correlation with the band free combination index rRVI (534, 546) (r=0.927). The correlation between the other photosynthetic pigment parameters and the corresponding spectral index was above 0.797. In the hyperspectral parameter- photosynthetic pigment-yield prediction model, the chlorophyll content, carotenoid content, canopy chlorophyll density, and canopy carotenoid density were used as connection points, and using the spectral indices of rNDVI (534, 546), rRVI (531, 555), rNDVI (532, 546), and rNDVI (531, 555) as independent variables, the PLS output prediction model had better effect (R2=0.509, RMSE=1.352 t·hm -2). 【Conclusion】 In intercropping maize, the pigment parameters were used as a bridge between spectral data and yield. A prediction model was established through PLS regression, which could achieve a better estimation of maize yield and provide the theoretical and technical reference for field management and growth monitoring of maize in intercropping.

Key words: relay intercropping, maize, hyperspectral, pigment parameters, yield, partial least squares regression

Table 1

Vegetation indexes and spectral indexes quoted in this paper"

植被指数及光谱指数
Vegetation index and spectral index
计算公式
Calculation
文献来源
Reference
叶绿素反演植被指数 Transformed chlorophyll absorption in reflectance index, TCARI 3×[(R700-R670)-0.2×(R700-R550)×(R700/R670)] [20]
绿度植被指数 Green normalized difference vegetation index, GNDVI (R800-R550)/(R800+R550) [20]
光化学植被指数 photochemical reflectance index, PRI (R531-R570)/(R531+R570) [21]
rDVI(Ri, Rj) Random difference vegetation index Ri-Rj
rRVI(Ri, Rj) Random ratio vegetation index Ri/Rj
rNDVI(Ri, Rj) Random normalized difference vegetation index (Ri-Rj)/(Ri+Rj)

Table 2

Yield difference in maturity period of each treatment (t·hm-2)"

处理
Treatment
拔节期Exp.1
Jointing stage
灌浆期Exp.1
Filling stage
拔节期Exp.4
Jointing stage
抽雄期Exp.4
Tasseling stage
灌浆期Exp.4
Filling stage
试验2
Exp.2
试验3
Exp.3
试验5
Exp.5
CK(N1) 7.113±0.825a 5.112±0.549a 4.483±0.124a 5.573±0.206a 4.296±0.466b 8.829±0.332a 7.021±0.075a
L(N2) 6.473±0.495a 4.739±0.657a 3.780±0.125bc 4.639±0.079a 5.343±0.679ab 7.409±0.505b 8.978±0.316a 7.119±0.518a
M(N3) 5.914±0.230ab 4.494±0.676a 4.102±0.222b 5.250±0.483a 5.907±0.148a 9.700±0.863a 9.854±0.477a 8.237±0.662a
S(N4) 4.355±0.232b 4.493±0.582a 3.496±0.138c 3.627±0.258b 5.919±0.146a 9.956±0.171a 8.181±0.476a

Table 3

Photosynthetic pigment parameters in each growth period"

生育时期 Growth period 试验组成 Test composition Ct (mg·cm-2) CCD (g·m-2) Car (mg·cm-2) CCarD (mg·m-2)
拔节期 Jointing stage Exp.(1-5) n=54 0.056±0.026 0.544±0.426 0.008±0.007 84.778±98.435
抽雄期 Tasseling stage Exp(3-5) n=33 0.107±0.068 4.615±3.588 0.012±0.005 502.782±299.183
灌浆期 Filling stage Exp(1-2,4-5) n=40 0.077±0.064 3.255±3.334 0.009±0.005 349.077±279.695
总样本 Total sample Exp.(1-5) n=127 0.076±0.056 2.457±3.127 0.009±0.006 276.637±286.108

Table 4

Correlation analysis of photosynthetic pigment parameters and yield"

生育时期 Growth period 试验组成 Test composition Ct CCD Car CCarD
拔节期 Jointing stage Exp.(1-5) n=54 -0.111 -0.240 -0.321* -0.358**
抽雄期 Tasseling stage Exp(3-5) n=33 -0.729** -0.663** -0.616** -0.565**
灌浆期 Filling stage Exp(1-2,4-5) n=40 -0.275 0.283 -0.342* -0.288
总样本 Total sample Exp.(1-5) n=127 -0.330** -0.295** -0.373** -0.287**

Table 5

Regression analysis between pigment parameters and yield in maize"

模型
Model
色素参数
Pigment parameter
函数
Function
R2 RMSE (t·hm-2) R2 RMSE (t·hm-2)
线性非线性模型
Linear and non-
linear model
Ct (mg·cm-2) y = -22.183x + 9.450 0.560 1.349 0.472 1.401
y = -280.49x2+ 47.191x + 6.5568 0.669 1.170 0.545 1.338
CCD (g·m-2) y = -0.383x + 8.8655 0.467 1.485 0.387 1.517
y = 0.006x2 - 0.4563x + 8.9976 0.468 1.484 0.392 1.510
Car (mg·cm-2) y = -238.06x + 9.9226 0.399 1.577 0.298 1.616
y = -45375x2 + 887.63x + 4.2694 0.646 1.209 0.477 1.437
CCarD (mg·m-2) y = -0.0039x + 9.079 0.345 1.646 0.269 1.657
y = -3E-06x2 + 0.0002x + 8.1775 0.364 1.636 0.264 1.691
偏最小二乘模型
PLS-M
Ct (mg·cm-2), CCD (g·m-2)
Car (mg·cm-2), CCarD (mg·m-2)
y=-79.7789x1-0.6549x2+529.075x3+0.0123x4+6.002
(x1:Ct, x2:CCD, x3:Car, x4:CCarD)
0.859 0.768 0.882 0.669

Fig. 1

Correlation analysis of photosynthetic pigment parameters with the original and first derivative spectra"

Table 6

Correlation analysis of photosynthetic pigment parameters with vegetation indexes"

光合色素参数
Photosynthetic pigment
parameter
叶绿素反演
植被指数TCARI
绿度植
被指数GNDVI
光化学
植被指数
PRI
Ct -0.138 0.090 0.185
CCD -0.184 0.095 0.212
Car -0.189 0.260* 0.118
CCarD -0.239* 0.128 0.164

Fig. 2

Correlation analysis of photosynthetic pigment parameters and rRVI"

Table 7

Correlation analysis of photosynthetic pigment parameters and spectral indexes"

光谱
Spectrum
色素参数
Pigment parameter
rDVI rRVI rNDVI
波长
Wavelength
相关系数
r
波长
Wavelength
相关系数
r
波长
Wavelength
相关系数
r
原始光谱
Original spectrum
Ct 1009,972 0.882** 534,546 0.927** 534,546 0.926**
CCD 1009,972 0.831** 531,555 0.912** 533,539 0.904**
Car 1011,962 0.853** 532,546 0.908** 532,546 0.908**
CCarD 1009,975 0.797** 531,555 0.895** 531,555 0.894**
一阶导数光谱
First derivative spectrum
Ct 1491,769 0.898** 544,449 0.865** 1895,1451 0.861**
CCD 1491,768 0.854** 544,448 0.868** 544,448 0.853**
Car 1491,769 0.892** 650,688 0.846** 1491,741 0.828**
CCarD 1491,763 0.857** 544,448 0.840** 544,448 0.826**

Table 8

Linear and non-linear regression analysis between photosynthetic pigment parameters and spectral indexes"

色素参数
Pigment parameter
光谱指数
Spectral index
模型
Model
函数
Function
建模集
Calibration set (n=90)
验证集
Test set (n=40)
R2 RMSE R2 RMSE
Ct
(mg·cm-2)
rDVI (1009,972) 线性 Linear y=0.0753x-0.0694 0.779 0.033 0.792 0.033
二次Quadratic y = -0.0043x2 + 0.0976x -0.0952 0.780 0.032 0.790 0.031
rRVI (534,546) 线性 Linear y=5.181x-4.612 0.859 0.026 0.820 0.028
二次Quadratic y = 39.363x2 - 66.684x + 28.183 0.866 0.025 0.812 0.029
rNDVI (534,546) 线性 Linear y=9.4714x+0.5494 0.857 0.026 0.820 0.028
二次Quadratic y = 145.48x2 + 22.759x +0.8462 0.867 0.025 0.813 0.029
CCD
(g·m-2)
rDVI (1009,972) 线性 Linear y=3.7883x-4.2894 0.690 2.058 0.695 1.930
二次Quadratic y = -0.0206x2 + 3.8945x -4.4126 0.690 2.191 0.538 2.476
rRVI (531,555) 线性 Linear y=168.25x-138.36 0.831 1.520 0.813 1.556
二次Quadratic y = 1211.3x2 - 1899.1x + 743.25 0.847 1.445 0.859 1.377
rNDVI (533,539) 线性 Linear y=697.07x+25.798 0.829 1.529 0.820 1.459
二次Quadratic y = 14598x2 + 1548.5x + 37.863 0.837 1.493 0.839 1.391
Car (mg·cm-2) rDVI (1011,962) 线性 Linear y=0.0046x+0.0008 0.727 0.003 0.667 0.003
二次Quadratic y = -0.0008x2 + 0.0088x -0.0041 0.749 0.003 0.677 0.003
rRVI (532,546) 线性 Linear y=0.3379x-0.2864 0.824 0.002 0.775 0.003
二次Quadratic y = -0.0011x2 + 0.3399x - 0.2873 0.824 0.002 0.789 0.003
rNDVI (532,546) 线性 Linear y=0.5999x+0.0493 0.824 0.002 0.776 0.003
二次Quadratic y = 0.8472x2 + 0.704x + 0.0524 0.824 0.002 0.790 0.003
CCarD (mg·m-2) rDVI (1009,975) 线性 Linear y=328.64x-259.68 0.635 186.720 0.641 175.510
二次Quadratic y = 17.113x2 + 243.8x - 164.28 0.637 186.426 0.671 174.162
rRVI (531,555) 线性 Linear y=13824x-11244 0.801 137.950 0.797 136.940
二次Quadratic y = 43623x2 - 60627x + 20505 0.804 136.888 0.824 130.002
rNDVI (531,555) 线性 Linear y=23718x+2432.8 0.800 138.340 0.792 151.290
二次Quadratic y = 156703x2 + 48613x + 3400.1 0.804 136.771 0.819 129.656

Fig. 3

Comparison of estimated and predicted photosynthetic pigment parameters in maize based on hyperspectral remote sensing"

Table 9

Yield estimation models of maize"

色素参数
Pigment parameter
光谱指数
Spectral index
函数 Function 建模集 Calibration set 验证集 Test set
光谱-色素参数
Spectrum-pigment
色素参数-产量
Pigment-yield
R2 RMSE (t·hm-2) R2 RMSE (t·hm-2)
Car rNDVI (532,546) y=0.5999x+0.0493 y = -45375x2 + 887.63x + 4.2694 0.526 1.217 0.485 0.965
Ct rNDVI (534,546) y = 145.48x2 + 22.759x +0.8462 y=-79.7789x1-0.6549x2+529.075x3+0.0123x4+6.002
(x1:Ct, x2:CCD, x3:Car, x4:CCarD)
0.596 1.304 0.509 1.352
CCD rRVI (531,555) y = 1211.3x2 - 1899.1x + 743.25
Car rNDVI (532,546) y = 0.8472x2 + 0.704x + 0.0524
CCarD rNDVI (531,555) y = 156703x2 + 48613x + 3400.1
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