Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (8): 1532-1544.doi: 10.3864/j.issn.0578-1752.2020.08.004

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Response of Non-Photochemical Quenching in Bundle Sheath Chloroplasts of Two Maize Hybrids to Drought Stress

LIU WenJuan1,CHANG LiJuan1,YUE LiJie2,SONG Jun1,ZHANG FuLi1,WANG Dong1,WU JiaWei1,GUO LingAn1,LEI ShaoRong1   

  1. 1 Center of Analysis and Testing, Sichuan Academy of Agricultural Sciences, Chengdu 610066
    2 Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066
  • Received:2019-09-04 Accepted:2019-12-11 Online:2020-04-16 Published:2020-04-29

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Abstract:

【Objective】 Non-photochemical chlorophyll fluorescence quenching (NPQ) of photosystem II (PSII) is the most rapid photoprotective mechanism of higher plants that responds to a changing environment. Maize has two distinctly different classes of chloroplasts in mesophyll and bundle sheath cells. In the present study, the drought tolerance of two maize hybrids was compared to explore the significance of non-photochemical quenching in bundle sheath chloroplasts to maize tolerance. 【Method】 The experiment was conducted with two maize hybrids, Chengdan 30 and Zhongyu 3, and consisted of three soil moisture regimes, including 70%-80% of field water capacity (FWC) (sufficient irrigation), 50%-60% FWC (moderate drought stress), and 35%-45% FWC (severe drought stress). Physiological and biochemical parameters of maize leaves, including relative water content, chlorophyll contents, reactive oxygen species (ROS) accumulation, lipid peroxidation, and gas exchange, were measured. Fv/Fm and NPQ of PSII in mesophyll and bundle sheath chloroplasts of two maize hybrids were investigated through a chlorophyll fluorescence kinetic microscope. The steady-state levels of PSII subunit S (PsbS) in mesophyll and bundle sheath cells were analyzed using method of western blotting. PSII complexes levels were detected by blue native PAGE. 【Result】 The stomatal conductance and transpiration rate of maize leaves decreased under drought stress. There were no remarkably difference in decline degree of stomatal index between Chengdan 30 and Zhongyu 3. However, under severe drought conditions, Chengdan 30 showed better leaf water status, lower ROS damages, and higher photosynthetic efficiency compared with Zhongyu 3. NPQ levels and PsbS contents in bundle sheath chloroplasts increased more markedly than that in mesophyll chloroplasts when maize plant suffered with drought treatment, which was especially outstanding in Chengdan 30. The PSII complexes contents of Zhongyu 3 reduced obviously under drought stress, while the steady-state levels of light-harvesting complex II (LHCII) trimer of Chengdan 30 enhanced after severe drought. 【Conclusion】 The responses of photosynthetic mechanism to stomatal limitation were no significant difference in two maize hybrids under drought stress. However, compared with Zhongyu 3, Chengdan 30 had a higher non-photochemical quenching capacity in bundle sheath chloroplasts, which might play a positive influence on its superior drought tolerance of non-stomatal limitation.

Key words: maize, drought stress, bundle sheath cells, photosystem II, non-photochemical quenching

Fig. 1

Leaf water status and lipid peroxidation in maize leaves under drought stress A: Plant phenotypes observation; B: Relative water content (RWC); C: Chlorophyll a and b contents (Chl a+b); D: Superoxide anion radicals (O2-) content; E: Hydrogen peroxide (H2O2) content; F: Electrolyte leakage; G: Malondialdehyde (MDA) content. CK, well watered control; MS, moderate drought stress; SS, severe drought stress. Each point represents the mean±SD (n=4). Different letters indicate significant differences among one maize hybrid under progressive drought treatments at 0.05 level. One or two asterisks indicate significant differences between two maize hybrids under drought stress at P<0.05, and P<0.01, respectively. The same as below"

Fig. 2

Gas exchange in maize leaves under drought stress A: Stomatal conductance (gs); B: Transpiration rate (E); C: Intercellular CO2 concentration (Ci); D: Net photosynthetic rate (A)"

Fig. 3

Measurement of Fv/Fm in mesophyll and bundle sheath chloroplasts of maize leaves under drought stress A: Imaging of Fv/Fm in mesophyll and bundle sheath chloroplasts; B: Quantification of Fv/Fm in mesophyll and bundle sheath chloroplasts. M, mesophyll chloroplasts; BS, bundle sheath chloroplasts. The same as below"

Fig. 4

Measurement of NPQ in mesophyll and bundle sheath chloroplasts of maize leaves under drought stress A: Imaging of NPQ in mesophyll and bundle sheath chloroplasts; B: Quantification of NPQ in mesophyll and bundle sheath chloroplasts"

Fig. 5

Steady-state level of PsbS protein in mesophyll and bundle sheath cells of maize leaves under drought stress A: Immunoblot analyses of PsbS in mesophyll cells (1.0 μg Chl); B: Immunoblot analyses of PsbS in bundle sheath cells (1.5 μg Chl); C: Quantification of PsbS immunoblot signal in mesophyll cells (the amount of the PsbS protein in mesophyll cells of Chengdan 30 maize under well watered condition was defined as 100%); D: Quantification of PsbS immunoblot signal in bundle sheath cells (the amount of the PsbS protein in bundle sheath cells of Chengdan 30 maize under well watered condition was defined as 100%). Coomassie blue staining (CBS) of protein samples were shown as the control"

Fig. 6

Steady-state levels of PSII complexes in mesophyll and bundle sheath cells of maize leaves under drought stress A: Membranes in mesophyll thylakoids were solubilized with 1% DDM and loaded onto 4%-12% acrylamide Blue-Native gel; B: Membranes in bundle sheath thylakoids were solubilized with 2% DDM and loaded onto 4%-12% acrylamide Blue-Native gel; C: Quantification of LHCII trimer in mesophyll thylakoids (the amount of the LHCII trimer under well watered condition was defined as 100%); D: Quantification of LHCII trimer in bundle sheath thylakoids (the amount of the LHCII trimer under well watered condition was defined as 100%)"

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