大麦籽粒蛋白质及其相关功能成分含量的QTL分析

doi:10.3864/j.issn.0578-1752.2017.02.001

Abstract

Abstract: 【Objective】The objective of this study is to identify the QTLs controlling protein content, total flavonoids content, gamma-aminobutyric acid (GABA) content in barley grains and analyze their correlation, which could be a theoretical basis for genetic modification, gene cloning and molecular assisted breeding of the functional barley. 【Method】Based on two-year experiment data, a barley SSR genetic linkage map constructed by a total of 193 RIL population derived from the cross between Ziguangmangluoerleng (female parent) and Schooner (male parent) and the Inclusive Composite Interval Mapping(ICIM) method was used to identify the QTLs of protein, total flavonoids and GABA content. The correlations among the contents of three components were also analyzed. 【Result】 The two parents and RIL population have significant differences in protein, total flavonoids, and GABA content, showed continuous variation and normal distribution, which are suitable for QTL mapping. The map covered 2224.29cM with a mean density of 16.48cM per locus, including 7 linkage groups and 135 marker loci. A total of 20 QTLs for protein content, total flavonoids content, GABA content were detected. Nine QTL for protein content were localized on chromosomes 1H, 2H, 4H, 6H and 7H. It explained phenotypic variation range from 4.11% to 18.86%, 3 major QTL were localized on 6H and 7H, which explained 13.30%, 15.45% and 18.86% phenotypic variance. Two of the same QTL loci were found that localized on 4H BMAG0740-BMAG0808 and 6H Ebmac0806-GBM1270 based on two-year experiment. Seven QTL for total flavonoids content were localized on chromosomes 2H, 5H, 6H and 7H. It explained phenotypic variation range from 6.06% to 29.01%, 5 major QTL were localized on 2H, 6H and 7H, which explained 10.38%, 15.27%, 17.55%, 24.17% and 29.01% phenotypic variance. One of the same QTL loci was found that localized on 7H EBmatc0016-Bmag0206 based on two-year experiment. Four QTL for GABA content were localized on chromosome 4H, 5H, 6H and 7H. It explained phenotypic variation range from 5.44% to 14.87%, the highest phenotypic variance was 14.87%, which localized on 7H. QTLs controlling protein content and total flavonoids content were mapped and mainly localized on 2H, 6H and 7H, QTLs controlling protein content and GABA content were mainly localized on 4H, 6H and 7H, QTLs controlling total flavonoids content and GABA content were mainly localized on 5H, 6H and 7H. So QTLs for three components were mainly localized on 6H and 7H, especially on the 6H Ebmac0806-GBM1270 interval, and they all affected protein, total flavonoids and GABA content, the contribution rates of additive effects were the same, and there was a significantly correlation. The correlation analysis showed that the significantly positive correlations were existed among protein, total flavonoids and GABA content. 【Conclusion】the correlations of protein content, total flavonoids content, GABA content are consistent with the correlations of QTLs controlling those components. It revealed the close genetic relationship between protein and functional components.

Key words: barley, grains, protein, total flavonoids, GABA, correlation, QTL

YANG XiaoMeng, DU Juan, ZENG YaWen, PU XiaoYing, YANG ShuMing, YANG Tao, WANG LuXiang, YANG I JiaZhen. QTL Mapping of Protein and Related Functional Components Content in Barley Grains[J].Scientia Agricultura Sinica, 2017, 50(2): 205-215.

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