Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (4): 807-815.doi: 10.3864/j.issn.0578-1752.2022.04.015

• ANIMAL SCIENCE·VETERINARY SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Effects of CaSR and CCK-1R Mediated Soybean Protein Hydrolysate on Appetite Using Mouse

WANG LÜYang(),CUI LeiHong,FENG JiangYin,HONG QiuXia,YOU MeiJing,BAO HaoYu,HANG SuQin()   

  1. National Center for International Research on Animal Gut Nutrition/Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health/Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095
  • Received:2020-12-24 Accepted:2021-05-13 Online:2022-02-16 Published:2022-02-23
  • Contact: SuQin HANG E-mail:2018105079@njau.edu.cn;suqinhang69@njau.edu.cn

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Abstract:

【Objective】The study aimed to investigate the effects and mechanisms of soy protein hydrolysate (SPH) on the appetite in mice, so as to provide the new frame work guidelines for strategies towards manipulating feed intake in pigs. 【Method】In this study, the pepsin was used to hydrolyze soy protein to produce SPH. Firstly, the effects on short-term feed intake and the expressions of duodenal peptide sensing receptors calcium sensing receptor (CaSR), G protein-coupled receptor 93 (GPR9)3 and oligopeptide transporter 1 (PepT1) were investigated by intragastrically different concentrations of SPH in mice. Based on this, the CaSR inhibitor NPS2143 and the peripheral cholecystokinin-1 receptor (CCK-1R) inhibitor Devazepide were intraperitoneally injected, respectively, to investigate whether SPH inhibited feed intake by the CASR-CCK-CCK-1R-hypothalamus pathway. 【Result】The amount of 1.5g·kg-1 SPH reduced the 0-1 h feed intake (P<0.05), and increased the CaSR expression (P<0.05). Compared with SPH group, the feed intake of SPH+NPS2143 group were increased at 0-1 h, and the plasma CCK levels were decreased, and there were no differences from the control group (0.05<P<0.5). Meanwhile, SPH reduced 0-1 h gastric emptying rate and increased the expression of hypothalamus anorexia nerve factor pro-opiomelanocortin (POMC) (P<0.05), while the effects disappeared in SPH+Devazepide group. However, SPH had no effect on the small intestine transit rate or the expression of the hypothalamic food-promoting factors neuropeptide Y (NPY) and agouti related peptide (AgRP). 【Conclusion】CaSR mediated SPH to promote CCK secretion, delayed gastric emptying rate through the peripheral CCK-1 receptor, and improved the expression of hypothalamic anorexia nerve factor POMC to suppress appetite.

Key words: soy protein hydrolysate, food intake, calcium sensing receptor, cholecystokinin, CCK-1 receptor, hypothalam

Fig. 1

The experiment timelines"

Table 1

Primer sequences of Real-Time PCR"

基因
Gene		 引物序列
Primer sequences (5'-3')		 参考文献
Reference
β-actin F: AAATCGTGCGTGACATCAAA
R: AAGGAAGGCTGGAAAAGAGC		 [15]
CaSR F: GCATCAGGTATAACTTCCGTGG
R: TTGGAGACGGTGTTACAGGTG
GPR93 F: GCTGTCGTCTATTCGTCTGGC
R: CGAAGCACAGCAGGAAGATG
PepT1 F: CCACGGCCATTTACCATACG
R: TGCGATCAGAGCTCCAAGAA		 [16]
POMC F: AAGAGGTTAAGAGCAGTGA
R: ACATCTATGGAGGTCTGAAG		 [17]
NPY F: CTAGGTAACAAGCGAATGG
R: GGTGATGAGATTGATGTAGTG
AGRP F: ACAAGAGACCAGGACATC
R: CAACAGCAGAACACAACT

Fig. 2

Effects of SPH on the feed intake and the expression of duodenum peptide sensing receptors/transporter in mice (n = 7-8) Control: Saline; SPH: 大豆蛋白水解物; “*” represents Control vs SPH and significant difference (P<0.05); different letters represent significant difference (P<0.05). The same as below"

Fig. 3

Effects of CaSR inhibitor on feed intake and CCK secretion in mice (n = 7-8)"

Fig. 4

Effects of CCK-1R inhibitor on feed intake, gastric emptying rate, small intestine transit and gene expression of appetite-related factors in hypothalamus in mice (n = 7-8) Devazepid: CCK-1R inhibitor; “#” represents SPH vs SPH+Devazepid and significant difference (P<0.05)"

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