基于优异等位基因的棉花抗黄萎病性状的分子鉴定

doi:10.3864/j.issn.0578-1752.2017.02.002

Abstract

Abstract: 【Objective】Detecting Verticillium wilt resistance in cotton by using molecular markers of elite alleles of Verticillium wilt resistance contributes to fast identification of disease resistance and direct selection of disease resistance genotypes, thus resolving the problems of time-consuming and poor efficiency in selection of disease resistance. 【Method】In this study, Verticillium wilt resistance of 125 elite cotton lines was estimated by using a disease nursery and greenhouse screening method, respectively. Elite alleles related to Verticillium wilt resistance were obtained from the earlier publication of the authors and the phenotypic effect of each elite allele was calculated. The number and the sum of effective value of elite alleles in each cotton line were used to study the possibility of elite alleles-based molecular detection for Verticillium wilt resistance. 【Result】Results of the study showed that widespread variation of Verticillium wilt resistance was observed in upland cotton. The range of relative disease index of Verticillium wilt resistance identified in field disease nursery and in greenhouse was 10.10-76.6 and 17.01-72.63, respectively. A total of 40 elite alleles were obtained and the effective values of each allele was in the range of -8.20--0.39. The number of elite alleles in each lines was in the range of 1-24. The sum of effective values of elite alleles in each line were in the range of -92.37--0.86. Correlation analysis showed that the sum of effective values of elite alleles had a significant positive correlation with relative disease index of Verticillium wilt resistance, and the correlation coefficient in field disease nursery and in greenhouse was 0.616 and 0.566, the number of elite alleles in each line had a significant negative correlation with relative disease index of Verticillium wilt resistance, and the correlation coefficient in field disease nursery and in greenhouse was -0.618 and -0.535, respectively. 【Conclusion】It was concluded that there is a significant correlation between the number of elite alleles, the sum of effective value of elite alleles and relative disease index of Verticillium wilt resistance, implying the accumulation of different elite alleles in one line can improve the disease resistance. The sum of effective value of elite alleles and the number of elite alleles in a cotton line can reflect the disease resistance, thus realizing the molecular identification of disease resistance in cotton.

Key words: cotton, Verticillium wilt, elite allele, molecular identification

ZHAO YunLei, WANG HongMei, CHEN Wei, GONG HaiYan, SANG XiaoHui, CUI YanLi, ZHAO Pei. Elite Alleles-Based Molecular Detection for Verticillium Wilt Resistance in Cotton[J].Scientia Agricultura Sinica, 2017, 50(2): 216-227.

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