昼夜变温下高温与干旱胁迫对Bt棉毒蛋白含量的影响及其生理机制

doi:10.3864/j.issn.0578-1752.2022.23.004

Abstract

Abstract:

【Objective】 This study was conducted to investigate the effects of alternating high temperature and drought on the content of Cry1Ac protein in Bt cotton and the underlying physiological mechanism, so as to provide a reference for the safe and stable utilization of insect resistance of Bt cotton in production.【Method】 The conventional cultivar Sikang 1 (SK-1) and hybrid cultivar Sikang 3 (SK-3) were used in 2019 and 2020 in experimentally controlled greenhouse, Yangzhou University. From 7:00 am to 7:00 pm, two high-temperature treatments [34℃ (A1) and 38℃ (A2)] were imposed on cotton plants, followed by an optimum temperature 28℃ during the remaining night hours. There were two treatments for soil moisture content, which were 50% (B1) and 60% (B2) field capacity. The treatment with 32℃/28℃ and 75% field capacity was set as the control (CK). The leaf Cry1Ac protein content and its physiological mechanism were detected on 4, 7, and 10 days after stress (DAS), respectively.【Result】 Compared with CK, the Cry1Ac protein content all decreased under the stresses of high temperature and drought, and with the extension of the stress time, greater decrease was observed. The extent of decline for A1B2 was the smallest, followed by A1B1, while A2B1 and A2B1 were the largest. The Cry1Ac protein content under A1B2 was significantly lower than CK after 7 DAS, while the significant differences between A1B1, A2B2, A2B2 and CK were detected after 4 DAS. The Bt gene expression level, soluble protein (SP), free amino acid (aa) contents, nitrate reductase (NR), the glutamic pyruvic transaminase (GPT), glutamic oxaloacetate transaminase (GOT), glutamine synthetase (GS) and glutamate synthase (GOGAT) activities showed a downward trend. While the tannin content, the activities of protease and peptidase showed an upward trend. The correlation analysis and path analysis showed SP, aa, NR, GPT, GOT, GS, and GOGAT were positively correlated with Cry1Ac protein content. The tannin content, activities of protease, and peptidase were negatively correlated with Cry1Ac protein content. NR, GPT and GS could be key indices for the Cry1Ac protein content.【Conclusion】 The interaction of high temperature and drought resulted in the decrease of Cry1Ac protein content in Bt cotton, with greater decrease observed as the stress extended. There was no significant difference between the treatment with 34℃/28℃ and 60% field capacity and CK in 7-10 DAS. The reduction extent of Cry1Ac for alternating high temperature regime and drought decreased and the period was delayed. NR, GPT and GS could be key indices for the Cry1Ac protein content.

Key words: Bt cotton, Cry1Ac protein, high temperature, drought, physiological mechanism

YIN YanYu, XING YuTong, WU TianFan, WANG LiYan, ZHAO ZiXu, HU TianRan, CHEN Yuan, CHEN Yuan, CHEN DeHua, ZHANG Xiang. Cry1Ac Protein Content Responses to Alternating High Temperature Regime and Drought and Its Physiological Mechanism in Bt Cotton[J].Scientia Agricultura Sinica, 2022, 55(23): 4614-4625.

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References 31

[1]	夏兰芹, 郭三堆. 高温对转基因抗虫棉中Bt杀虫基因表达的影响. 中国农业科学, 2004, 37(11): 1733-1737.
	XIA L Q, GUO S D. The expression of Bt toxin gene under different thermal treatments. Scientia Agricultura Sinica, 2004, 37(11): 1733-1737. (in Chinese)
[2]	CHEN D H, YE G Y, YANG C Q, CHEN Y. The effect of high temperature on the insecticidal properties of Bt cotton. Environmental and Experimental Botany, 2005, 53(3): 333-342. doi: 10.1016/j.envexpbot.2004.04.004
[3]	BENEDICT J H, SACHS E S, ALTMAN D W, DEATON W R, KOHEL R J, RING D R, BERBERICH S A. Field performance of cottons expressing transgenic Cry1A insecticidal proteins for resistance to Heliothis virescens and Helicoverpa zea (Lepidoptera: Noctuidae). Journal of Economic Entomology, 1996, 89(1): 230-238. doi: 10.1093/jee/89.1.230
[4]	MARTINS C M, BEYENE G, HOFS J L, KRUGER K, VYVER C V D, SCHLUTER U, KUNERT K J. Effect of water-deficit stress on cotton plants expressing the Bacillus thuringiensis toxin. Annals of Applied Biology, 2008, 152(2): 255-262. doi: 10.1111/j.1744-7348.2007.00214.x
[5]	张祥, 王剑, 彭盛, 芮秋治, 李丽楠, 陈媛, 陈源, 陈德华. 温度和土壤水分与Bt棉铃壳中杀虫蛋白表达关系及其氮代谢生理机制. 中国农业科学, 2018, 51(7): 1261-1271.
	ZHANG X, WANG J, PENG S, RUI Q Z, LI L N, CHEN Y, CHEN Y, CHEN D H. Relationship between temperature, soil moisture, and insecticidal protein content in Bt cotton boll shell and the mechanism of nitrogen metabolism. Scientia Agricultura Sinica, 2018, 51(7): 1261-1271. (in Chinese)
[6]	STAM M, MOL J N M, KOOTER J M. The silence of genes in transgenic plants. Annals of Botany, 1997, 79(1): 3-12
[7]	ALLAH B, SAIMA S, TAYYAB H. A molecular approach to combat spatio-temporal variation in insecticidal gene (Cry1Ac) expression in cotton. Euphytica, 2012, 183(1): 65-74 doi: 10.1007/s10681-011-0497-8
[8]	SHINJI I, TAKESHI S, MADOKA N, YASUHISA K. Influence of tannic acid on the insecticidal activity of a Bacillus thuringiensis serovar aizawai formulation against Spodoptera litura fabricius (Lepidoptera: Noctuidae). Biological Control, 2021, 157(1): 104558 doi: 10.1016/j.biocontrol.2021.104558
[9]	CHEN Y, LIU Z Y, TAMBEL L, ZHANG X, CHEN Y, CHEN D H. Reduced square Bacillus thuringiensis insecticidal protein content of transgenic cotton under N deficit. Journal of Integrative Agriculture, 2021, 20(1):100-108. doi: 10.1016/S2095-3119(20)63190-2
[10]	WANG J, CHEN Y, YAO M H, LI Y, WEN Y J, CHEN Y, ZHANG X, CHEN D H. The effects of high temperature level on square Bt protein concentration of Bt cotton. Journal of Integrative Agriculture, 2015, 14(10): 1971-1979. doi: 10.1016/S2095-3119(15)61049-8
[11]	陈松, 吴敬音, 何小兰, 黄骏麒, 周宝良, 张荣铣. 转基因抗虫棉组织中Bt毒蛋白表达量的ELISA测定. 江苏农业学报, 1997(3): 27-29.
	CHEN S, WU J Y, HE X L, HUANG J Q, ZHOU B L, ZHANG R X. Quantification using ELISA of Bacillus thuringiensis insecticidal protein expressed in the tissue of transgenic insect-resistant cotton. Jiangsu Journal of Agriculture Science, 1997(3): 27-29. (in Chinese)
[12]	ZHANG X, WANG J, PENG S, LI Y, ZHANG L Y, CHEN Y, CHEN D H. Effects of soil water deficit on insecticidal protein expression in boll shells of transgenic Bt cotton and the mechanism. Frontiers in Plant Science, 2017, 8: 1-11.
[13]	邹琦. 植物生理学实验指导. 北京: 中国农业出版社, 2000: 127-130.
	ZOU Q. Experimental Instruct of Plant Physiology. Beijing: China Agriculture Press, 2000: 127-130. (in Chinese)
[14]	邵金良, 黎其万, 董宝生, 刘宏程, 束继红. 茚三酮比色法测定茶叶中游离氨基酸总量. 中国食品添加剂, 2008(2):162-165.
	SHAO J L, LI Q W, DONG B S, LIU H C, SHU J H. Determination of total free-amino acid in tea by Nihydrin colorimetry. China Food Additives, 2008(2): 162-165. (in Chinese)
[15]	HOWARD T. Regulation of alanine aminotransferase in leaves of Lolium temulentum during senescence. Zeitschrift fü Pflanzenphysiologie, 1975, 74(3): 208-218.
[16]	赵鹏, 何建国, 熊淑萍, 马新明. 氮素形态对专用小麦旗叶酶活性及籽粒蛋白质和产量的影响. 中国农业大学学报, 2010, 15(3): 29-34.
	ZHAO P, HE J G, XIONG S P, MA X M. Studies on the effects of different nitrogen forms on enzyme activity in flag leaves in wheat and protein and yield of grain for specialized end-uses. Journal of China Agricultural University, 2010, 15(3): 29-34. (in Chinese)
[17]	李文才, 林振武, 汤玉玮. 硝酸还原酶的研究——Ⅶ.棉花不同品种的硝酸还原酶活力. 作物学报, 1986, 12(2): 95-100.
	LI W C, LIN Z W, TANG Y W. Studies on nitrate reductase VII. Nitrate reductase activities in different cotton cultivars. Acta Agronomica Sinica, 1986, 12(2): 95-100. (in Chinese)
[18]	HU W, ZHAO W Q, YANG J S, OOSTERHUIS D M, LOKA D A, ZHOU Z G. Relationship between potassium fertilization and nitrogen metabolism in the leaf subtending the cotton (Gossypium hirsutum L.) boll during the boll development stage. Plant Physiology and Biochemistry, 2016, 101: 113-123. doi: 10.1016/j.plaphy.2016.01.019
[19]	杨宇晖, 张青文, 刘小侠. 棉花营养物质和单宁含量与其对绿盲蝽抗性的关系. 中国农业科学, 2013, 46(22): 4688-4697.
	YANG Y H, ZHANG Q W, LIU X X. The relationship between the contents of nutrients and tannins in different cotton varieties and their resistance to Apolygus lucorum. Scientia Agricultura Sinica, 2013, 46(22): 4688-4697. (in Chinese)
[20]	ZHANG X, RUI Q Z, LIANG P P, WEI C H, DENG G Q, CHEN Y, DONG Z D, CHEN D H. Dynamics of Bt cotton Cry1Ac protein content under an alternating high temperature regime and effects on nitrogen metabolism. Journal of Integrative Agriculture, 2018, 17(9): 1991-1998. doi: 10.1016/S2095-3119(17)61878-1
[21]	刘震宇, 王桂霞, 李丽楠, 蔡泽洲, 梁潘潘, 吴莘玲, 张祥, 陈德华. 高温胁迫终止后Bt棉蕾杀虫蛋白的恢复特征及相关生理机制. 作物学报, 2020, 46(3): 440-447. doi: 10.3724/SP.J.1006.2020.94080
	LIU Z Y, WANG G X, LI L N, CAI Z Z, LIANG P P, WU X L, ZHANG X, CHEN D H. Recovery characteristics of Bt insecticidal protein and relative physiology mechanisms after high temperature stress termination in square of Bt cotton. Acta Agronomica Sinica, 2020, 46(3): 440-447. (in Chinese) doi: 10.3724/SP.J.1006.2020.94080
[22]	CHEN S L, LI P, TAN S L, PU X J, ZHOU Y, HU K M, HUANG W, LIU L. Combined proteomic and physiological analysis of chloroplasts reveals drought and recovery response mechanisms in Nicotiana benthamiana. Plants-Basel, 2021, 10(6): 1127.
[23]	LIU Z Y, ELTAYIB H M A A, WU H, ZHOU M Y, ZHANG X, CHEN Y, CHEN D H. Bt insecticidal efficacy variation and agronomic regulation in Bt cotton. Journal of Cotton Research, 2019, 2(1): 1-6. doi: 10.1186/s42397-018-0018-6
[24]	MAIKE S, JOSEPH N M M, JAN M K. The silence of genes in transgenic plants. Annals of Botany, 1997, 79(1): 3-12.
[25]	陆宴辉, 杨益众, 余月书. 棉花单宁的研究简介. 中国棉花, 2004(5): 21-22.
	LU Y H, YANG Y Z, YU Y S. Review of the cotton tannin. China Cotton, 2004(5): 21-22. (in Chinese)
[26]	陈德华, 杨长琴, 陈源, 聂安全, 吴云康. 高温胁迫对Bt棉叶片杀虫蛋白表达量和氮代谢影响的研究. 棉花学报, 2003, 15(5): 288-292.
	CHEN D H, YANG C Q, CHEN Y, NIE A Q, WU Y K. The effects of the high temperature stress on the leaf Bt protein content and nitrogen metabolism of Bt cotton. Cotton Science, 2003, 15(5): 288-292. (in Chinese)
[27]	YANG J, LIN L, ZHANG L L, WANG K, LI X X, CAI M L, ZHAN M, LI C F, WANG J P, CAO C G. Comparison of transgenic Bt rice and their non-Bt counterpart in yield and physiological response to drought stress. Field Crops Research, 2018, 217: 45-52. doi: 10.1016/j.fcr.2017.12.007
[28]	OLSEN K M, DALY J C, FINNEGAN E J, MAHON R J. Changes in Cry1Ac Bt transgenic cotton in response to two environmental factors: Temperature and insect damage. Journal of Economic Entomology, 2005, 98(4): 1382-1390. doi: 10.1603/0022-0493-98.4.1382 pmid: 16156594
[29]	STULEN I, KOCH B T, KOSTER A. An endogenous inhibitor of nitrate reductase in radish cotyledons. Acta Botanica Neerlandica, 1971, 20(4): 389-396. doi: 10.1111/j.1438-8677.1971.tb00724.x
[30]	SAIF A, ABDUL H, MA X L, TUNG S A, CHATTHA M S, SHAH A N, LUO D, AHMAD S, LIU J H, YANG G Z. Equal potassium- nitrogen ratio regulated the nitrogen metabolism and yield of high-density late-planted cotton (Gossypium hirsutum L.) in Yangtze River valley of China. Industrial Crops and Products, 2019, 129: 231-241. doi: 10.1016/j.indcrop.2018.12.009
[31]	ELTAYIB H M A, YUAN L, HEN L, CHEN Y, LEILA I M, HU D P, ZHANG X, CHEN D H. Amino acid composition and level affect Bt protein concentration in Bt cotton. Plant Growth Regulation, 2017, 82(3): 439-446. doi: 10.1007/s10725-017-0270-7

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年份 Year	品种 Cultivar	处理 Treatment	胁迫后天数Days after stress (DAS)
年份 Year	品种 Cultivar	处理 Treatment	4 d	7 d	10 d
2019	SK-1	CK	894.29±5.56a	670.72±2.62a	558.93±16.4a
		A1B1	422.30±1.60abc	345.67±82.45bc	227.52±42.32b
		A1B2	598.00±4.66ab	438.14±3.77b	434.73±34.66a
		A2B1	111.75±1.45c	83.81±1.45d	60.16±9.53c
		A2B2	274.57±2.66bc	219.62±9.61cd	181.69±2.82bc
2020	SK-1	CK	835.63±14.62ab	826.02±5.32a	810.17±0.34b
		A1B1	424.98±40.69def	343.43±52.67de	294.50±1.93f
		A1B2	654.28±26.05abcd	387.93±4.16cde	366.11±2.79e
		A2B1	104.20±2.40g	93.39±2.04g	33.62±2.06i
		A2B2	255.69±2.02fg	214.81±1.70f	114.88±0.66h
	SK-3	CK	873.93±16.69a	859.89±17.97a	892.94±3.23a
		A1B1	606.27±60.58bcde	502.56±58.28bc	464.49±1.64d
		A1B2	705.66±25.49abc	605.46±58.32b	523.92±4.19c
		A2B1	370.71±3.29ef	313.82±2.61ef	282.39±1.88g
		A2B2	481.85±1.99cdef	457.19±1.64cd	368.96±7.99e

品种 Cultivar	处理 Treatment	胁迫后天数Days after stress (DAS)
品种 Cultivar	处理 Treatment	4 d	7 d	10 d
SK-1	CK	3.15±0.20c	3.81±0.25d	4.04±0.01ef
	A1B1	3.84±0.09bc	4.84±0.61c	5.38±0.14c
	A1B2	3.17±0.02c	3.95±0.04d	4.96±0.04cd
	A2B1	5.50±1.03a	6.17±0.19a	8.01±1.18a
	A2B2	4.49±0.50b	5.45±0.24b	6.73±0.61b
SK-3	CK	0.73±0.13g	0.77±0.07g	1.15±0.06h
	A1B1	1.76±0.22de	1.94±0.05f	3.87±0.07f
	A1B2	1.02±0.11fg	1.53±0.01f	1.77±0.04h
	A2B1	2.25±0.31d	2.51±0.36e	4.61±0.30de
	A2B2	1.51±0.47ef	1.82±0.22f	2.80±0.07g

年份 Year	品种 Cultivar	处理 Treatment	SP含量 SP content			aa含量 aa content
年份 Year	品种 Cultivar	处理 Treatment	4 DAS	7 DAS	10 DAS	4 DAS	7 DAS	10 DAS
2019	SK-1	CK	2.60±0.20a	2.02±0.05a	1.14±0.05a	1.50±0.10a	1.05±0.04a	0.91±0.02a
		A1B1	1.42±0.00bc	0.97±0.04c	0.67±0.03c	0.98±0.07b	0.82±0.05bc	0.64±0.04c
		A1B2	2.21±0.11ab	1.68±0.23b	0.97±0.02b	1.15±0.20b	0.90±0.08ab	0.70±0.00b
		A2B1	0.72±0.01c	0.36±0.05e	0.34±0.02e	0.67±0.02c	0.54±0.01d	0.53±0.03d
		A2B2	1.34±0.08bc	0.62±0.01d	0.55±0.06d	0.95±0.01b	0.69±0.16cd	0.54±0.03d
2020	SK-1	CK	5.36±0.19b	4.42±0.10b	2.5±0.19b	1.81±0.47a	1.37±0.06a	1.06±0.04a
		A1B1	4.70±0.13cd	3.35±0.03e	1.17±0.02f	1.22±0.11b	1.03±0.10c	0.81±0.02c
		A1B2	5.03±0.14bc	3.76±0.07d	1.74±0.08d	1.42±0.08ab	1.18±0.08b	0.92±0.02b
		A2B1	3.57±0.22f	2.23±0.08h	0.63±0.02h	0.69±0.04c	0.68±0.01d	0.62±0.04e
		A2B2	4.20±0.17e	2.99±0.30f	0.95±0.04g	1.12±0.04bc	0.92±0.10c	0.72±0.01d
	SK-3	CK	5.81±0.53a	4.73±0.06a	3.08±0.02a	2.19±0.02a	1.85±0.02a	1.50±0.05a
		A1B1	4.83±0.05c	3.36±0.03e	1.34±0.01e	1.88±0.10b	1.34±0.06b	1.01±0.02c
		A1B2	5.27±0.28b	3.98±0.08c	2.11±0.03c	2.06±0.16ab	1.39±0.04b	1.10±0.02b
		A2B1	3.65±0.01f	2.44±0.02g	0.91±0.04g	1.59±0.04c	0.98±0.18c	0.80±0.03e
		A2B2	4.33±0.03de	3.17±0.03ef	1.15±0.03f	1.66±0.12c	1.10±0.07c	0.89±0.04d

年份 Year	品种 Cultivar	处理 Treatment	GPT活性 GPT Activity			GOT活性 GOT Activity
年份 Year	品种 Cultivar	处理 Treatment	4DAS	7DAS	10DAS	4DAS	7DAS	10DAS
2019	SK-1	CK	22.31±3.57a	21.29±0.14a	18.65±0.11a	31.02±5.34a	27.57±2.13a	26.19±0.70a
		A1B1	18.88±0.15bc	16.82±0.80c	10.48±0.25c	25.93±0.21b	23.83±024b	19.95±0.57c
		A1B2	20.57±2.87ab	18.91±0.25b	14.01±0.22b	27.05±2.64ab	26.47±0.63ab	24.57±0.64b
		A2B1	16.56±0.36d	10.24±0.35e	5.42±0.20e	22.33±0.34b	18.45±0.23d	13.03±0.36e
		A2B2	17.41±0.15cd	12.52±0.23d	8.69±0.16d	25.31±0.21b	20.91±0.10c	17.09±0.25d
2020	SK-1	CK	24.05±3.48c	22.81±1.73c	20.64±0.63b	36.55±2.32c	35.14±0.36c	29.84±0.89c
		A1B1	18.65±2.48f	14.81±0.80e	11.10±0.35g	31.71±2.25d	26.20±0.52e	20.19±0.10f
		A1B2	22.70±1.06cd	18.51±0.86d	12.89±0.82f	34.84±1.55c	30.95±1.54d	25.82±0.40e
		A2B1	13.01±0.21h	7.42±0.15g	4.25±0.18j	24.13±0.82f	19.90±0.41f	14.21±0.04g
		A2B2	16.19±0.30g	10.73±0.19f	9.60±0.73i	27.03±0.37e	21.45±0.14f	18.53±0.36f
	SK-3	CK	30.79±0.37a	29.70±0.38a	26.23±0.28a	45.76±1.54a	41.33±1.30a	40.71±0.43a
		A1B1	28.67±0.17b	22.24±1.09c	16.59±0.68d	39.80±2.54b	36.01±1.61c	28.06±1.07d
		A1B2	29.92±1.46ab	24.57±0.84b	19.57±0.63c	43.99±1.38a	38.69±0.93b	35.98±0.67b
		A2B1	20.85±0.08e	15.76±0.54e	10.37±0.36h	31.88±1.38d	26.41±1.25e	19.67±1.67f
		A2B2	22.47±0.29d	18.12±0.58d	15.04±1.18e	34.48±2.14cd	31.81±1.47d	26.28±1.78e

年份 Year	品种 Cultivar	处理 Treatment	GS活性 GS activity (U·g^-1 FW·min^-1)			GOGAT活性 GOGAT activity (μmol·g^-1 FW·min^-1)
年份 Year	品种 Cultivar	处理 Treatment	4DAS	7DAS	10DAS	4DAS	7DAS	10DAS
2019	SK-1	CK	7.35±0.30a	6.61±0.28a	5.95±0.31a	3.36±0.43a	3.19±0.02a	3.03±0.04a
		A1B1	4.96±0.10ab	3.42±0.06bc	3.31±0.45c	2.33±0.00abc	2.01±0.09bc	1.93±0.03b
		A1B2	5.28±0.11ab	4.73±0.09b	3.86±0.04b	2.55±0.15ab	2.27±0.14ab	2.01±0.26b
		A2B1	3.63±0.09b	2.32±0.24c	1.58±0.39e	1.33±0.11c	1.26±0.04c	0.90±0.04c
		A2B2	4.63±0.08b	2.88±0.11c	2.17±0.32d	2.00±0.12bc	1.94±0.04bc	1.74±0.19bc
2020	SK-1	CK	8.68±0.25c	7.17±0.11bc	6.21±0.07b	3.41±0.19d	3.16±0.02c	2.80±0.24d
		A1B1	8.21±0.06d	5.09±0.30de	4.27±0.19e	3.12±0.17e	2.22±0.10f	1.55±0.01f
		A1B2	8.47±0.20cd	6.59±0.40c	5.66±0.32c	3.30±0.10de	2.95±0.24d	2.50±0.15e
		A2B1	5.46±0.32f	3.71±0.17g	1.43±0.29h	1.80±0.15g	1.65±0.07h	0.98±0.02h
		A2B2	6.89±0.10e	4.33±0.29f	2.5±0.35g	2.25±0.05f	1.97±0.08g	1.32±0.03g
	SK-3	CK	10.29±0.21a	9.32±0.44a	7.31±0.25a	4.91±0.09a	4.49±0.17a	4.53±0.11a
		A1B1	9.84±0.09b	7.58±0.68b	5.18±0.07d	4.15±0.08b	3.97±0.17b	3.24±0.02c
		A1B2	9.88±0.09ab	8.74±0.38a	6.49±0.32b	4.76±0.11a	4.34±0.03a	3.55±0.05b
		A2B1	6.87±0.36e	4.87±0.12ef	2.48±0.18g	3.22±0.07de	2.44±0.09e	1.65±0.10f
		A2B2	8.37±0.39cd	5.70±0.10d	3.31±0.15f	3.65±0.02c	3.11±0.03cd	2.36±0.09e

Cry1Ac Protein Content Responses to Alternating High Temperature Regime and Drought and Its Physiological Mechanism in Bt Cotton

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