Scientia Agricultura Sinica ›› 2016, Vol. 49 ›› Issue (8): 1466-1475.doi: 10.3864/j.issn.0578-1752.2016.08.004


Effects of Shade Degrees on Photosynthesis and Lodging Resistance Degree of Different Shade Tolerance Soybean

LIU Ting, LIU Wei-guo, REN Meng-lu, DU Yong-li, DENG Yu-chuan, ZOU Jun-lin, FANG Ping, YANG Wen-yu   

  1. Institute of Ecological Agriculture, Sichuan Agricultural University/Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu 611130
  • Received:2015-10-08 Online:2016-04-16 Published:2016-04-16

Abstract: 【Objective】Under the maize/soybean relay strip intercropping system, shading from high crop maize leads to soybean seedling thinning, lodging and decreasing soybean yield. We studied the effect of soybean photosynthesis and lodging resistance in seeding stage under different shade degrees in the maize/soybean relay strip intercropping system, then provided a theoretical basis for the establishment of a reasonable community population structure. 【Method】 The non-shade tolerance and non-lodging resistant Nan032-4 and the strong shade tolerance and strong lodging resistance Nandou 12 were taken as experimental materials in 2014 and 2015. We designed three light conditions in the outdoor pod experiment, the natural light (W0), one layer of black sun-shade net (W1), and two layers of black sun-shade net (W2) with light transmittances of 100%, 32% and 11% respectively. Photosynthetic characteristics such as soybean photosynthetic rate, stomatal conductance, transpiration rate, intercellular CO2 concentration,SPAD values, leaf area and biomass accumulation of each organ and lodging characters, including plant height, each internode length, each internode diameter, stem snapping resistance, lodging resistance index were measured and analyzed. 【Result】 The results showed that as the shade increased, photosynthetic rate, stomatal conductance, leaf area and SPAD values, the dry matter accumulation of each organ and root/shoot ratio of the two soybean varieties were all reduced significantly. As shade increased, seedling lodging became serious, with the stem snapping resistance and lodging resistance index also reduced significantly. However, the shade avoidance responses on different shade degrees were significantly different between these two different shade tolerant varieties. Compared with W0, photosynthetic rates of the shade tolerant cultivar Nandou12 and the non-shade tolerance Nan032-4 under W1 and W2 were decreased by 35.6%, 99.6% and by 40.9%, 83.6% respectively. Compared with Nandou12, Nan032-4 was influenced more seriously. The dry matter accumulation of Nandou12 under W0 and W1was higher than Nan032-4, but under W2 there was no significance between the two varieties (P<0.05), this indicated that the photosynthetic rate of soybean remained at a higher level under moderate shade and the photosynthetic product of the shade tolerant cultivar was higher than the non-shade tolerance cultivar. The lodging rate over 2014 and 2015 of Nan032-4 under W1 and W2 was higher than that of Nandou12 by 55.9% and 2.57%. Under the treatment W1, Nan032-4 showed moderate lodging and Nandou12 showed mild lodging; Nan032-4 while Nandou12 showed serious lodging under the treatment W2. Under the same shade level, the snapping resistance of Nandou12 (39.8%) was higher than Nan 032-4 (38.4%), but the snapping resistance of Nandou12 under W1 and W2 for 2014 and 2015 was higher than that of Nan032-4 by 27.3% and 26.6%. Under the same shade level, stem snapping resistance and lodging resistance index of the shade-tolerant cultivar Nandou12 were much higher than that of Nan032-4 (P<0.05).【Conclusion】With increasing shade level, photosynthetic accumulation of soybean was reduced and lodging was more serious. The two materials showed the shade sensitive under the excessive shade. But under moderate shade, the strong shade tolerant cultivar maintained its high photosynthetic and lodging resistance. In a word, under the maize/soybean relay strip intercropping system, we should appropriately reduce the shading stress on soybean in production, then finally realize soybean potential of shade tolerance and lodging resistance.

Key words: soybean, shade tolerance, shade degree, photosynthetic, lodging

[1]    杨文钰, 雍太文, 任万军, 樊高琼, 牟锦毅, 卢学兰. 发展套作大豆, 振兴大豆产业. 大豆科学, 2008, 27(1): 1-7.
Yang W Y, Yong T W, Ren W J, Fan G Q, Mou J Y, Lu X L. Develop relay-planting soybean, revitalize soybean industry. Soybean Science, 2008, 27(1): 1-7. (in Chinese )
[2]    雍太文, 杨文钰, 任万军, 樊高琼, 牟锦毅. 发展套作大豆,促进四川大豆产业发展. 作物杂志, 2007(6): 5-8.
Yong T W, Yang W Y, Ren W J, Fan G Q, Mou J Y. Develop relayplanting soybean, promote the development of soybean industry in Sichuan. Crops7(6): 5-8. (in Chinese ), 200
[3]    刘广才. 不同间套作系统种间营养竞争的差异性及其机理研究[D]. 兰州: 甘肃农业大学, 2005.
Liu G C. Difference and its mechanism of interspecific nutrition competition in different intercropping systems [D]. Lanzhou: Gansu Agricultural University, 2005. (in Chinese )
[4]    Sposaro M M, Berry P M, Sterling M, Halla A J, Chimentia C A. Modelling root and stem lodging in sunflower. Field crops Research, 2010, 119(1): 125-134.
[5]    李金才, 尹钧, 魏凤珍. 播种密度对冬小麦茎秆形态特征和抗倒指数的影响. 作物学报, 2005, 31(5): 662-666. 
Li J C, Ying J, Wei F Z. Effects of planting density on characters of culm and culm lodging resistant index in winter wheat. Acta Agronomica Sinica, 2005, 31(5): 662-666. (in Chinese)
[6]    伍晓燕. 不同株型玉米对套作大豆形态生理及产量品质影响的研究[D]. 雅安: 四川农业大学, 2007.
Wu X Y. Effects of different plant-type maize on morphological and physiological characters and yield and quality of relay-cropping soybean [D]. Ya’an: Sichuan Agricultural University, 2007. (in Chinese )
[7]    吴其林, 王竹, 杨文钰. 苗期遮荫对大豆茎秆形态和物质积累的影响. 大豆科学, 2007, 26(6): 868-872.
Wu Q L, Wang Z, Yang W Y. Seeding shading affects morphogenesis and substance accumulation of stem in soybean. Soybean Science, 2007, 26(6): 868-872. (in Chinese )
[8]    Yang F, Huang S, Gao R C, Liu W G, Yong T W, Wang X C, Wu X L,Yang W Y. Growth of soybean seedlings in relay strip intercropping systems in relation to light quantity and red: Far-red ratio. Field Crops Research, 2014(155): 245-253.
[9]    宋艳霞, 杨文钰, 李卓玺, 于晓波, 郭凯, 向达兵. 不同大豆品种幼苗叶片光合及叶绿素荧光特性对套作遮阴的响应. 中国油料作物学报, 2009, 31(4): 474-479.
Song Y X, Yang W Y, Li Z X, Yu X B, Guo K, Xiang D B. The effects of shading on photosynthetic and fluorescent characteristics of soybean seedlings under mazie-soybean relay cropping. Chinese Journal of Oil Crop Sciences, 2009, 31(4): 474-479. (in Chinese )
[10]   王竹, 杨文钰, 吴其林. 玉/豆套作遮蔽对大豆光合特性与产量的影响. 作物学报, 2007, 33(9): 1502-1507.
Wang Z, Yang W Y, Wu Q L. Effects of shading in maize/soybean relay-cropping system on the photosynthetic characteristics and yield of soybean. Acta Agronomica Sinica, 2007, 33(9): 1502-1507. (in Chinese)
[11]   Charlotte M M, Gommers C M, Eric J W, Visser E J, St Onge K R, Laurentius A C J, Voesenek L A. Pierik R. Shade tolerance: When growing tall is not anoption. Trends in Plant Science, 2013, 18(2): 65-71.
[12]   于晓波, 张明荣, 吴海英, 杨文钰. 净套作下不同耐荫性大豆品种农艺性状及产量分布的研究. 大豆科学, 2012, 31(5): 757-761.
Yu X B, Zhang M R, Wu H Y, Yang W Y. Agronomic characters and yield distribution of different shade tolerance soybean under monoculture and relay strip intercropping systems. Soybean Science, 2012, 31(5): 757-761. (in Chinese )
[13]   Umezaki T, Yoshida T. Effect of shading on the internode elongation of late maturing soybean. Journal of the Faculty of Agriculture, kyushu University, 1992, 36(3/4): 267-272.
[14]   陈怀珠, 孙祖东, 杨守臻, 李初英. 荫蔽对大豆主要性状的影响及大豆耐荫性鉴定方法研究初报. 中国油料作物学报. 2003, 25(4): 78-82.
Chen H Z, Sun Z D, Yang S Z, Li C Y. Effect of shading on major characters of soybean and preliminary study on the identification method of soybean shade endurance. Chinese Journal of Oil Crop Sciences, 2003, 25(4): 78-82. (in Chinese )
[15]   李春红, 姚兴东, 鞠宝韬, 朱明月, 王海英, 张惠君, 于翠梅, 谢甫娣, 宋书宏. 不同基因型大豆耐荫性分析及其鉴定指标的筛选. 中国农业科学, 2014, 7(15): 2927-2939.
Li C H, Yao X D, Ju B T, Zhu M Y, Wang H Y, Zhang H J, Yu C M, Xie F T, Song S H. Analysis of shade-tolerance and determinationof shade-tolerance evaluation indicators in different soybean genotypes. Scientia Agricultura Sinica, 2014, 47(15): 2927-2939. (in Chinese)
[16]   武晓玲, 梁海媛, 杨峰, 刘卫国, 余跃辉, 杨文钰. 大豆苗期耐荫性综合评价及其鉴定指标的筛选. 中国农业科学, 2015, 48(13): 2427-2507.
Wu X L, Liang H Y, Yang F, Liu W G, Yu Y W, Yang W Y. Analysis of shade-tolerance and determinationof shade-tolerance evaluation indicators of soybean. Scientia Agricultura Sinica, 2015, 48(13): 2427-2507. (in Chinese)
[17]   Liu W G, Jiang T, Zhou X R, Yang W Y. Charaterisitics of expansins in soybean (Glycine max) internodes and responses to shade stress. Asian Journal of Crop Science, 2011, 3(1): 26-34.
[18]   刘卫国, 蒋涛, 佘跃辉, 杨峰, 杨文钰. 大豆苗期茎秆对荫蔽胁迫响应的生理机制初探. 中国油料作物学报, 2011, 33(2): 141-146.
Liu W G, Jiang T, She Y H, Yang F, Yang W Y. Preliminary study on physiological response mechanism of soybean (Glycine max) stem to shade stress at seedling stage. Chinese Journal of Oil Crop Sciences, 2011, 33(2): 141-146. (in Chinese)
[19]   Liu W G, Zou J L, Zhang J, Yang F, Wan Y, Yang W Y. Evaluation of soybean (Glycine max) stem vining in maize–soybean relay strip intercropping system. Plant Production Science, 2015, 18: 69-75.
[20]   邹俊林, 刘卫国, 袁晋, 蒋涛, 叶素琴, 邓榆川, 杨晨雨, 罗玲, 杨文钰. 套作大豆苗期茎秆木质素合成与抗倒性的关, 作物学报, 2015, 41(7): 1098-1104.
Zou J L, Liu W G, Yuan J, Jiang T, Ye S Q, Deng Y C, Yang C Y, Luo L, Yang W Y. Relationship between ligninsynthesis and lodging resistance at seedlings stage in soybean intercropping system. Acta Agronomica Sinica, 2015, 41(7): 1098-1104. (in Chinese)
[21]   罗玲, 于晓波, 万燕, 蒋涛, 杜俊波, 邹俊林, 杨文钰, 刘卫国. 套作大豆苗期倒伏与茎秆内源赤霉素代谢的关系. 中国农业科学, 2015, 48(13): 2528-2537.
Luo L, Yu X B, Wan Y, Jiang T, Du J B, Zou J L, Yang W Y, Liu W G. The relationship between lodging and stem endogenous gibberellins metabolism pathway of relay intercropping soybean at seedling stage. Scientia Agricultura Sinica, 2015, 48(13): 2528-2537. (in Chinese)
[22]   杨峰, 崔亮, 黄山, 刘卫国, 雍太文, 杨文钰. 不同株型玉米套作大豆生长环境动态及群体产量研究. 大豆科学, 2015, 34(3): 402-407. 
Yang F, Cui L, Huang S, Liu W G, Yong T W, Yang W Y. Soybean growth environment and group yield in soybean relay intercropped with different leaf type maize. Soybean Science, 2015, 34(3): 402-407. (in Chinese)
[23]   Gong W Z, Qi P F, Du J B,Sun X, Wu X L,Song C, Liu W G, Wu Y S, Yu X B, Yong T W, Wang X C, Yang F, Yan Y H, Yang W Y. Transcriptome analysis of shade-induced inhibition on leaf size in relay intercropped soybean. PLoS One, 2014, 9(6): e98465.
[24]   邱丽娟, 常汝镇. 大豆种质资源描述规范和数据标准. 北京: 中国农业出版社, 2006: 60-61.
Qiu L J, Chang R Z. Descriptors and Date Standard for Soybean (Glycine spp.). Beijing: China Agriculture Press, 2006: 60-61. (in Chinese)
[25]   周蓉, 王贤智, 张晓娟, 沙爱华, 吴学, 涂赣英, 邱德珍, 周新. 大豆种质倒伏抗性价方法研究. 大豆科学, 2007, 26(4): 485-489.
Zhou R, Wang X Z, Zhang X J, Sha A H, Wu X J, Tu G Y, Qiu D Z, Zhou X A. Evaluation methed of lodging resistance in soybean germplasm. Soybean Science, 2007, 26(4): 485-489. (in Chinese)
[26]   黄中文, 赵团结, 喻德跃, 陈受宜, 盖钧镒. 大豆抗倒伏的评价指标及其QTL分析. 作物学报, 2008, 34(4): 605-611.
Huang Z W, Zhao T J, Yu D X, Chen S Y, Gai J Y. Lodging resistance indices and related QTLs in soybean. Acta Agronomica Sinica, 2008, 34(4): 605-611. (in Chinese)
[27]   王建林, 于贵瑞, 王伯伦, 齐华, 徐正进. 北方粳稻光合速率、气孔导度对光强和CO2浓度的响应. 植物生态学报, 2005, 29(1): 16-25.
Wang J L, Yu G R, Wang B L, Qi H, Xu Z J. Response of photosynthetic rate and stomatal conductance of rice to light intensity and CO2 concentration in northern China. Acta Phytoecologica Sinice, 2005, 29(1): 16-25. (in Chinese)
[28]   崔亮, 苏本营, 杨峰, 杨文钰. 不同玉米-大豆带状套作组合条件下光合有效辐射强度分布特征对大豆光合特性和产量的影响. 中国农业科学, 2014, 47(8): 1489-1501.
Cui L, Su B Y, Yang F, Yang W Y. Effects of photosynthetic active radiation intensity distribution characteristics under different maize/soybean system on photosynthetic characteristics and yield of soybean. Scientia Agricultura Sinica, 2014, 47(8): 1489-1501. (in Chinese)
[29]   Sharkey T D. Transpiration-induced change in the photosynthetic capacity of leaves. Planta, 1984, 160(2): 143-150.
[30]   田绍仁, 孙亮庆, 夏绍南, 崔爱花, 吴香华, 柯兴盛, 杨磊. 不同密度下湘杂棉8号干物质积累及动态分配. 江西棉花, 2009, 31(2): 3-5.
Tian S R, Sun L Q, Xia S N, Cui A H, Wu X H, Ke X S, Yang L. Dry matter accumulationd and distribution of Xiangza cotton eight under diffierent density. Jiangxi Cotton, 2009, 31(2): 3-5. (in Chinese)
[31]   陈喜凤, 孙宁, 谷岩, 何文安, 贾恩吉, 赵福林, 杨巍, 吴春胜, 王振民. 不同群体结构下大豆植株抗倒性能的比较. 华南农业学报, 2015, 36(1): 33-34.
Cheng X F, Sun N, Gu A, He W A, Jia E J, Zhao F L, Yang W, Wu C S, Wang Z M. Compare lodging resistance of soybean under different population structure. (in Chinese)South China Agricultural Journal, 2015, 36(1): 33-34.
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