Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (12): 2347-2359.doi: 10.3864/j.issn.0578-1752.2022.12.007

• PLANT PROTECTION • Previous Articles     Next Articles

Light Energy Utilization and Response of Chlorophyll Synthesis Under Different Light Intensities in Mikania micrantha

JIN MengJiao1,2(),LIU Bo2(),WANG KangKang2,ZHANG GuangZhong2,QIAN WanQiang2(),WAN FangHao1,2()   

  1. 1College of Plant Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong
    2Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences/Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture/Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Shenzhen 518120, Guangdong
  • Received:2021-12-03 Accepted:2021-12-27 Online:2022-06-16 Published:2022-06-23
  • Contact: WanQiang QIAN,FangHao WAN E-mail:jinmj_97@163.com;liubo03@caas.cn;qianwanqiang@caas.cn;wanfanghao@caas.cn

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Abstract:

【Objective】Light is one of the important ecological factors for photosynthesis of plants. The capture and utilization of light by photosynthetic pigments affects the growth and development of plants, and then affects their survival and fitness in natural ecosystems. The objective of this study is to clarify the photosynthetic physiological characteristics and response of gene expression of chlorophyll biosynthesis pathway to different light intensities in Mikania micrantha, as well as the relationship between photosynthetic energy and chlorophyll transformation, and to provide physiological and ecological evidences for explaining the “rapid growth” of M. micrantha.【Method】The photosynthetic pigment content of M. micrantha was determined by ethanol extraction under different light intensities (0, 20%, 40% and 100%). The variation pattern of chlorophyll a and b ratio (Chl a/b) was analyzed. The photosynthetic characteristics of M. micrantha were compared with the representative plants of different photosynthetic pathways (C3, C4 and CAM). The contents of ATP and starch in M. micrantha leaves under the above light intensities were determined by micro-method and anthrone colorimetric method. The cDNA library of M. micrantha under different light intensities was established and sequenced. Bioinformatics software including OrthoFinder, Blastp, HISAT2, StringTie, R package were used to analyze the expression pattern of genes involved in chlorophyll synthesis and light-harvesting complex (LHC) in M. micrantha under different light intensities. The expression patterns of genes related to chlorophyll synthesis pathway were identified.【Result】At 100% light intensity, the Chl b and carotenoid contents and Chl a/b in M. micrantha leaves were similar to those in C4 plant (maize). The Chl a/b in M. micrantha and maize leaves was significantly higher than that in C3 (rice and tomato) and CAM (aloe) plants. The contents of Chl a, Chl b and carotenoid in M. micrantha leaves under different light intensities did not change significantly, but Chl a/b significantly increased with increasing light intensity. Between the 40% and 100% light intensity, ATP content in M. micrantha leaves changed slightly, while starch content in M. micrantha leaves increased significantly in 100% light intensity. When the light intensity was 0, starch content decreased sharply and ATP content increased. The gene expression levels of HEMA, CHLH, CRD1 and CAO gene families involved in chlorophyll biosynthesis in M. micrantha were regulated by light induction, and the expression levels of light-harvesting complex (LHC) genes were higher under high light intensity.【Conclusion】Under different light conditions, M. micrantha may regulate the synthesis of Chl a and Chl b, and the mutual transformation of starch and ATP, which lay the foundation for the higher photosynthetic rate and light adaptability of M. micrantha.

Key words: Mikania micrantha, photosynthetic pigment characteristics, ATP content, starch storage, response to different light environments, regulation of gene expression

Fig. 1

Variation curves of diurnal photoperiod light intensity settings for five species"

Fig. 2

The changes of photosynthetic pigment content and Chl a/b in different photosynthetic pathways plants Different lowercases on the bars represent significant difference at P<0.05 level among different plants with the same index. Five biological replicates were set for each species"

Fig. 3

Changes of photosynthetic pigment content and Chl a/b in M. micrantha under different light intensities Different lowercases represent significant difference at P<0.05 level. The same as Fig. 4"

Fig. 4

Changes of ATP and starch contents in M. micrantha under different light intensities"

Table 1

Expression levels of gene families in chlorophyll biosynthesis pathway under different light intensities"

基因家族
Gene family		 基因名称
Gene ID		 不同时间下的光照强度 Light intensity under different times
8：00 (40%) 12：00 (100%) 17：00 (20%) 18：00 (0)
HEMA Mm03G008458 265.7 3069.0 516.0 505.3
Mm04G009926 1.0 8.3 19.0 12.3
Mm04G009947 0.3 9.0 29.3 6.3
Mm09G022271 1165.3 796.3 950.0 1128.7
HEMC Mm03G007750 1651.0 1452.3 2872.0 2340.3
Mm15G034118 326.0 279.7 741.3 636.0
Mm15G034127 309.7 283.0 774.3 608.0
HEMD Mm19G040701 549.0 542.0 628.0 722.0
Mm19G040704 292.67 181.0 282.7 359.3
Mm19G040708 207.3 107.0 118.3 147.3
Mm19G040711 142.3 86.3 79.3 104.7
HEME Mm01G003424 1007.7 810.3 2560.0 2159.0
Mm07G017442 324.3 427.7 1019.3 875.7
Mm14G033554 1.3 3.7 5.3 7.0
HEMF Mm04G008958 4639.3 3728.0 6053.0 5493.3
GUN4 Mm06G015460 11.0 20.0 25.0 13.3
Mm06G015530 6.0 12.7 14.7 13.0
CHLH Mm13G030916 9896.7 15342.3 2337.0 2011.3
MmUnG043782 6985.3 10106.3 1522.3 1202.3
CHLI Mm18G039053 2991.7 5393.0 5682.7 5434.0
Mm01G002914 3245.3 2445.0 3985.0 3772.0
CRD1 Mm13G030861 2430.3 2990.7 423.3 386.7
Mm13G030884 3649.3 4209.0 594.7 483.3
PCB Mm17G037161 216.0 472.3 710.7 513.0
POR Mm01G002138 814.0 3970.3 5360.0 4283.0
Mm03G008274 32.0 1323.7 3327.3 4707.0
Mm04G009104 28.7 73.0 117.3 94.0
Mm07G016864 12.7 40.7 104.7 113.0
Mm16G036000 16.3 12.3 45.0 17.0
MmUnG042187 17.7 14.0 60.3 26.7
MmUnG044337 0.3 0.7 1.3 0.3
G4 Mm07G017733 17.3 4.0 34.7 14.7
Mm17G037705 3177.7 2369.0 3596.0 3518.0
CLH Mm06G015923 107.0 116.3 276.7 51.3
Mm07G018083 430.0 857.3 966.7 785.0
MmUnG042738 1.7 2.7 5.3 2.0
CAO Mm01G001296 67.3 276.3 165.7 63.0
Mm01G001298 2.0 3.3 4.0 1.3
Mm02G004540 5003.7 2837.0 421.0 518.3
Mm05G012908 4965.3 4202.7 2773.3 2253.7
Mm08G021299 1425.3 2045.7 549.3 573.0
Mm16G036155 706.7 757.7 1119.3 911.7

Table 2

Gene expression levels of light-harvesting complex (LHC) protein family under different light intensities"

基因家族
Gene family		 基因名称
Gene ID		 不同时间下的光照强度 Light intensity under different times
8：00 (40%) 12：00 (100%) 17：00 (20%) 18：00 (0)
LHCB2 Mm01G002159 182.0 4514.0 138.0 174.0
Mm01G002160 15.0 689.0 20.7 109.7
Mm07G019027 6013.7 27098.0 1747.7 1522.7
Mm18G039443 8876.3 79479.0 15727.0 5623.7
MmUnG042961 24.0 873.7 50.7 130.7
MmUnG042962 1025.0 9108.3 427.0 466.7
Mm11G026494 660.7 8919.0 3085.7 3943.7
LHCB3 Mm16G035290 425.0 23868.7 6085.0 5605.7
MmUnG044047 7463.0 36412.7 3480.3 6033.0
Mm12G028787 713.7 5819.0 784.3 1731.0
Mm12G028786 33.0 109.3 57.0 64.0
Mm07G019026 4625.3 15617.3 2046.7 2507.7
LHCB5 Mm19G040589 6216.3 24846.7 9970.7 9191.3
Mm11G026495 2.0 183.7 54.7 270.3
Mm11G026493 246.3 3081.3 1335.7 2221.0
Mm10G024129 593.3 12951.7 1605.0 784.7
Mm11G026496 264.0 6615.3 1325.7 2067.3
LIL3 Mm04G009004 3726.0 2896.0 5061.7 4622.0
Mm12G028071 1768.3 1584.3 2006.3 1737.0

Fig. 5

Variation of gene expression levels at different time points in regulating chlorophyll biosynthesis pathway The changes of expression levels at different times are shown in the form of heat maps, in which, from left to right, the gene expression levels of M. micrantha related enzymes regulating chlorophyll biosynthesis under different light intensities at 8：00, 12：00, 17：00 and 18：00, respectively"

Fig. 6

Heat map of the light-harvesting complex (LHC) protein family gene expression changes under different light intensities"

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