植物TGA转录因子研究进展

doi:10.3864/j.issn.0578-1752.2016.04.003

Abstract

Abstract: TGA family is an important group of bZIP transcription factors in plant, which regulates the downstream target genes by binding to the as-1 cis-elements in the promoter region and subsequently activating or inhibiting their expression, ?nally regulating the resistance or development of floral organ. The first TGA gene was identified in tobacco. Subsequently, TGA members were widely characterized in arabidopsis, rice and apple. In arabidopsis, they constitute a multigene family comprising 10 members based on genome sequence analyses. The members of TGA family can be divided into five subgroups (Ⅰ: TGA1 and TGA4; Ⅱ: TGA2, TGA5 and TGA6; Ⅲ: TGA3 and TGA7; Ⅳ: TGA9 and TGA10; Ⅴ: PAN) according to their sequence similarity. The members of Ⅰ, Ⅱ and Ⅲ subgroups are mainly involved in disease resistance. Yeast two-hybrid and pull-down results showed that all members of subgroupⅠ, Ⅱ, and Ⅲ could interact with NPR1 protein which is the key positive regulator in SA signaling pathway. EMSA results indicated that the interaction between TGA and NPR1 could enhance the expression of pathogenesis-related (PR) genes and disease resistance. However, their roles were different in basal and systemic acquired resistance (SAR): tga3 mutants showed a defect in basal resistance, whereas the induced resistance was unaffected. TGA1 and TGA4 were involved in regulation of basal resistance and SAR. The tga2/5/6 triple mutants but not tga6 or tga2/5 double mutants were defective in SAR, a phenotype similar to that of the npr1 mutants, indicating functional redundancy among TGA2, TGA5 and TGA6. Yeast two-hybrid screen revealed that TGA transcription factors of subgroup Ⅱ could interact with glutaredoxin GRX480 to mediate the repression of the marker genes of JA signaling by SA, and they also could interact with GRAS protein SCL14 to increase the expression of CYP81D11 and GSTU7 in an NPR1-independent manner and enhance the tolerance to xenobiotics. In addition, the primary and lateral root growth was affected in tga1/4 and nrt2.1/2.2 mutants. ChIP and yeast one-hybrid assay results showed that TGA1 and TGA4 regulated the nitrate response via binding to the promoters of NRT2.1 and NRT2.2 to regulate their expression. TGA3 was also involved in the long-distance transport of cadmium. The members of subgroup Ⅳ and Ⅴ played an importance role in the regulation of floral organ development. tga9/10 mutants have defects in male gametogenesis that were similar to those in roxy1/2 mutants. PAN could interact with NPR1-like proteins BOP1 and BOP2. Both pan and bop1/2 double mutants revealed a pentamerous arrangement of sepals, which suggested that a similar signaling mechanism might be used between floral organ development and disease resistance. Finally, the research directions of TGA transcription factors was discussed, which will provide a reference for researchers in this field.

Key words: TGA transcription factors, nonexpressor of pathogenesis-related genes 1, resistance, development

TIAN Yi, ZHANG Cai-xia, KANG Guo-dong, LI Wu-xing, ZHANG Li-yi, CONG Pei-hua. Progress on TGA Transcription Factors in Plant[J].Scientia Agricultura Sinica, 2016, 49(4): 632-642.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2016.04.003

https://www.chinaagrisci.com/EN/Y2016/V49/I4/632

References

[1] Jakoby M. bZIP transcription factors in Arabidopsis. Trends in Plant Science, 2002, 7(3): 106-111.

[2] Lam E, Benfey P N, Gilmartin P M, Fang R X, Chua N H. Site- specific mutations alter in vitro factor binding and change promoter expression pattern in transgenic plants. Proceedings of the National Academy of Sciences of the USA, 1989, 86(20): 7890-7894.

[3] Redman J, Whitcraft J, Johnson C, Aris J. Abiotic and biotic stress differentially stimulate as-1 element activity in Arabidopsis. Plant Cell Reports, 2002, 21(2): 180-185.

[4] Pape S, Thurow C, Gatz C. The Arabidopsis PR-1 promoter contains multiple integration sites for the coactivator NPR1 and the repressor SNI1. Plant Physiology, 2010, 154(4): 1805-1818.

[5] Katagiri F, Lam E, Chua N H. Two tobacco DNA-bind proteins with homology to the nuclear factor CREB. Nature, 1989,340: 723-730.

[6] Xiang C, Miao Z, Lam E. DNA-binding properties, genomic organization and expression pattern of TGA6, a new member of the TGA family of bZIP transcription factors in Arabidopsis thaliana. Plant Molecular Biology, 1997, 34(3): 403-415.

[7] Kesarwani M, Yoo J, Dong X. Genetic interaction of TGA transcription factors in the regulation of pathogenesis-related genes and disease resistance in Arabidopsis. Plant Physiology, 2007, 144(1): 336-346.

[8] Gatz C. From Pioneers to team players: TGA transcription factors provide a molecular link between different stress pathways. Molecular Plant-Microbe Interactions, 2013, 26(2): 151-159.

[9] Niggeweg R, Thurow C, Kegler C, Gatz C. Tobacco transcription factor TGA2.2 is the main component of as-1-binding factor ASF-1 and is involved in salicylic acid-and auxin-inducible expression of as-1-containing target promoters. Journal of Biological Chemistry, 2000, 275(26): 19897-19905.

[10] Chern M S, Fitzgerald H A, Yadav R C, Canlas P E, Dong X, Ronald P C(2001). Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis. The Plant Journal, 2001, 27(2): 101-113.

[11] Espín F M I, Peraza-Echeverria S, Fuentes G, Santamaria J M. In silico cloning and characteriaztion of the TGA (TGACG MOTIF- BINDING FACTOR) transcription factors subfamily in Carica papaya. Plant Physiology and Biochemistry, 2012, 54: 113-122.

[12] Zhang J Y, Qu S C, Du X L, Qiao Y S, Cai B H, Guo Z R, Zhang Z. Overexpression of the Malus hupehensis MhTGA2 gene, a noval bZIP transcription factor for increased tolerance to salt and osmotic stress in transgenic tobacco. International Journal of Plant Sciences, 2012, 173(5): 441-453.

[13] Després C, DeLong C, Glaze S, Liu E, Fobert P R. The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors. The Plant Cell, 2000, 12(2): 279-290.

[14] Zhang Y, Tessaro M J, Lassner M, Li X. Knockout analysis of Arabidopsis transcription factors TGA2, TGA5, and TGA6 reveals their redundant and essential roles in systemic acquired resistance. The Plant Cell, 2003, 15(11): 2647-2653.

[15] Chuang C F, Running M, Williams R W, Meyerowitz E M. The PERIANTHIA gene encodes a bZIP protein involved in the determination of floral organ number in Arabidopsis thaliana. Genes & Development, 1999, 13(3): 334-344.

[16] Hepworth S R, Zhang Y, McKim S, Li X, Haughn G W. BLADE- ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis. The Plant Cell, 2005, 17(5): 1434-1448.

[17] Li S, Lauri A, Ziemann M, Busch A, Bhave A, Zachgo S. Nuclear activity of ROXY1, a glutaredoxin interacting with TGA factors, is required for petal development in Arabidopsis thaliana. The Plant Cell, 2009, 21(2): 429-441.

[18] Murmu J, Bush M J, DeLong C, Li S, Xu M, Khan M, Malcolmson C, Fobert P R, Zachgo S, Hepworth S R. Arabidopsis basic leucine- zipper transcription factors TGA9 and TGA10 interact with floral glutaredoxins ROXY1 and ROXY2 and are redundantly required for anther development. Plant Physiology, 2010, 154(3): 1492-1504.

[19] Yan S, Dong X. Perception of the plant immune signal salicylic acid. Current Opinion in Plant Biology, 2014, 20: 64-68.

[20] Herrera-Vásquez A, Salinas P, Holuigue L. Salicylic acid and reactive oxygen species interplay in the transcriptional control of defense genes expression. Frontiers in Plant Science, 2015, 6: 171.

[21] Uknes S, Mauch-Mani B, Moyer M, Potter S, Williams S, Dincher S, Chandler D, Slusarenko A, Ward E, Ryals J. Acquired resistance in Arabidopsis. The Plant Cell, 1992, 4(6): 645-656.

[22] Eulgem T. Regulation of the Arabidopsis defense transcriptome. Trends in Plant Science, 2005, 10(2): 71-78.

[23] Lebel E, Heifetz P, Thorne L, Uknes S, Ryals J, Ward E. Functional analysis of regulatory sequences controlling PR-1 gene expression in Arabidopsis. The Plant Journal, 1998, 16(2): 223-233.

[24] Zhang Y, Fan W, Kinkema M, Li X, Dong X. Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene. Proceedings of the National Academy of Sciences of the USA, 1999, 96(11): 6523-6528.

[25] Zhou J M, Trifa Y, Silva H, Pontier D, Lam E, Shah J, Klessig D F. NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid. Molecular Plant-Microbe Interactions, 2000, 13(2): 191-202.

[26] Johnson C, Boden E, Arias J. Salicylic acid and NPR1 induce the recruitment of trans-activating TGA factors to a defense gene promoter in Arabidopsis. The Plant Cell, 2003, 15(8): 1846-1858.

[27] Shearer H L, Wang L, DeLong C, Despres C, Fobert P R. NPR1 enhances the DNA binding activity of the Arabidopsis bZIP transcription factor TGA7. Botany, 2009, 87(6): 561-570.

[28] Wu Y, Zhang D, Chu J Y, Boyle P, Wang Y, Brindle I D, Luca V D, Després C. The Arabidopsis NPR1 protein is a receptor for the plant defense hormone salicylic acid. Cell Reports, 2012, 1(6): 639-647.

[29] Després C, Chubak C, Rochon A, Clark R, Bethune T, Desveaux D, Fobert P R. The Arabidopsis NPR1 disease resistance protein is a novel cofactor that confers redox regulation of DNA binding activity to the basic domain/leucine zipper transcription factor TGA1. The Plant Cell, 2003, 15(9): 2181-2191.

[30] Mou Z, Fan W, Dong X. Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes. Cell, 2003, 113(7): 935-944.

[31] Fu Z Q, Yan S, Saleh A, Wang W, Ruble J, Oka N, Mohan R, Spoel S H, Tada Y, Zheng N, Dong X. NPR3 and NPR4 are receptors for the immune signal salicylic acid in plants. Nature, 2012, 486(7402): 228-232.

[32] Weigel R R, Bäuscher C, Pfitzner A J P, Pfitzner U M. NIMIN-1, NIMIN-2 and NIMIN-3, members of a novel family of proteins from Arabidopsis that interact with NPR1/NIM1, a key regulator of systemic acquired resistance in plants. Plant Molecular Biology, 2001, 46(2): 143-160.

[33] Weigel R R, Pfitzner U M, Gatz C. Interaction of NIMIN1 with NPR1 modulates PR gene expression in Arabidopsis. The Plant Cell, 2005, 17(4): 1279-1291.

[34] Thibaud-Nissen F, Wu H, Richmond T, Redman J C, Johnson C, Green R, Arias J, Town C D. Development of Arabidopsis whole-genome microarrays and their application to the discovery of binding sites for the TGA2 transcription factor in salicylic acid-treated plants. The Plant Journal, 2006, 47(1): 152-162.

[35] Fonseca J P, Menossi M, Thibaud-Nissen F, Town C D. Functional analysis of a TGA factor-binding site located in the promoter region controlling salicylic acid-induced NIMIN-1 expression in Arabidopsis. Genetics and Molecular Research, 2010, 9(1): 167-175.

[36] Lindermayr C, Sell S, Muller B, Leister D, Durner J. Redox regulation of the NPR1-TGA1 system of Arabidopsis thaliana by nitric oxide. The Plant Cell, 2010, 22(8): 2894-2907.

[37] Shearer H L, Cheng Y T, Wang L, Liu J, Boyle P, Despres C, Zhang Y, Li X, Fobert P R. Arabidopsis clade I TGA transcription factors regulate plant defenses in an NPR1-independent fashion. Molecular Plant-Microbe Interactions, 2012, 25(11): 1459-1468.

[38] Rochon A, Boyle P, Wignes T, Fobert P R, Despres C. The coactivator function of Arabidopsis NPR1 requires the core of its BTB/POZ domain and the oxidation of C-terminal cysteines. The Plant Cell, 2006, 18(12): 3670-3685.

[39] Boyle P, Le Su E, Rochon A, Shearer H, Murmu J, Chu J Y, Fobert P R, Després C. The BTB/POZ domain of the Arabidopsis disease resistance protein NPR1 interacts with the repression domain of TGA2 to negate its function. The Plant Cell, 2009, 21(11): 3700-3713.

[40] 田义. 苹果抗病相关基因MdTGA2.1、MdAP2D4与MdAP2D19的克隆与分析[D]. 泰安: 山东农业大学, 2013.

Tian Y. Cloning and characterization of apple disease resistant-related genes MdTGA2.1, MdAP2D4, and MdAP2D19[D]. Taian: Shandong Agricultural University, 2013. (in Chinese)

[41] Thurow C, Schiermeyer A, Krawczyk S, Butterbrodt T, Nickolov K, Gatz C. Tobacco bZIP transcription factor TGA2.2 and related factor TGA2.1 have distinct roles in plant defense responses and plant development. The Plant Journal, 2005, 44(1): 100-113.

[42] Van Verk M C, Neeleman L, Bol J F, Linthorst H J M. Tobacco transcription factor NtWRKY12 interacts with TGA2.2 in vitro and in vivo. Frontiers in Plant Science, 2011, 2: 32.

[43] Pieterse C M J, Van der Does D, Zamioudis C, Leon-Reyes A, van Wees S C M. Hormonal modulation of plant immunity. Annual Review of Cell and Developmental Biology, 2012, 28: 489-521.

[44] Ndamukong I, Abdallat A A, Thurow C, Fode B, Zander M, Weigel R, Gatz C. SA-inducible Arabidopsis glutaredoxin interacts with TGA factors and suppresses JA-responsive PDF1.2 transcription. The Plant Journal, 2007, 50(1): 128-139.

[45] Zander M, Camera S L, Lamotte O, Metraux J P, Gatz C. Aarbidopsis thaliana class-ⅡTGA transcription factors are essential activators of jasmonic acid/ethylene-induced defense responses. The Plant Journal, 2010, 61(2): 200-210.

[46] Zarei A, Körbes A P, Younessi P, Montiel G, Champion A, Memelink J. Two GCC boxes and AP2/ERF-domain transcription factor ORA59 in jasmonate/ethylene-mediated activation of the PDF1.2 promoter in Arabidopsis. Plant Molecular Biology, 2011, 75(4/5): 321-331.

[47] Zander M, Thurow C, Gatz C. TGA transcription factors activate the salicylic acid-suppressible branch of the ethylene-induced defense program by regulating ORA59 expression. Plant Physiology, 2014, 165(4): 1671-1683.

[48] Buttner M, Singh K B. Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein. Proceedings of the National Academy of Sciences of the USA, 1997, 94(11): 5961-5966.

[49] Van Verk M C, Gatz C, Linthorst H J M. Transcriptional regulation of plant defense responses. Advances in Botanical Research, 2009, 51: 397-438.

[50] Choi J, Huh S U, Kojima M. The Cytokinin-activated transcription factor ARR2 promotes plant immunity via TGA3/NPR1-dependent salicylic acid signaling in Arabidopsis. Developmental Cell, 2010, 19(2): 284-295.

[51] Stotz H U, Mueller S, Zoeller M, Mueller M J, Berger S. TGA transcription factors and jasmonate-independent COI1 signalling regulate specific plant responses to reactive oxylipins. Journal of Experimental Botany, 2013, 64(4): 963-975.

[52] Xiang C, He Z, Lam E. Coordinated activation of as-1-type elements and a tobacco glutathione S-transferase gene by auxins, salicylic acid, methyl-jasmonate and hydrogen peroxide. Plant Molecular Biology, 1996, 32(3): 415-426.

[53] Chern W, Singh K B. The auxin, hydrogen peroxide and salicylic acid induced expression of the Arabidopsis GST6 promoter is mediated in part by an ocs element. The Plant Journal, 1999, 19(6): 667-677.

[54] Frova C. The plant glutathione transferase gene family: Genomic structure, function, expression and evolution. Physiologia Plantarum, 2003, 119(4): 469-479.

[55] Fode B, Siemsen T, Thurow C, Weigel R, Gatz C. The Arabidopsis GRAS protein SCL14 interacts with classⅡ TGA transcription factors and is essential for the activation of stress-inducible promoters. The Plant Cell, 2008, 20(11): 3122-3135.

[56] Köster J, Thurow C, Kruse K, Meier A, Iven T, Feussner I, Gatz C. Xenobiotic-and jasmonic acid-inducible signal transduction pathways have become interdependent at the Arabidopsis CYP81D11 promoter. Plant Physiology, 2012, 159(1): 391-402.

[57] Jayakannan M, Bose J, Babourina O, Shabala S, Massart A, Poschenrieder C, Rengel Z. The NPR1-dependent salicylic acid signalling pathway is pivotal for enhanced salt and oxidative stress tolerance in Arabidopsis.Journal of Experimental Botany, 2015, 66(7): 1865-1875.

[58] Du X, Du B, Chen X, Zhang S, Zhang Z, Qu S. Overexpression of the MhTGA2 gene from crab apple (Malus hupehensis) confers increased tolerance to salt stress in transgenic apple (Malus domestica). The Journal of Agricultural Science, 2014, 152(4): 634-641.

[59] Zhang J Y, Qu S C, Qiao Y S, Zhang Z, Guo Z R. Overexpression of the Malus hupehensis MhNPR1 gene increased tolerance to salt and osmotic stress in transgenic tobacco. Molecular Biology Reports, 2014, 41(3): 1553-1561.

[60] Matthews B F, Beard H, Brewer E, Kabir S, MacDonald M H, Youssef R M. Arabidopsis genes, AtNPR1, AtTGA2 and AtPR-5, confer partial resistance to soybean cyst nematode (Heterodera glycines) when overexpressed in transgenic soybean roots. BMC Plant Biology, 2014, 14(1): 96.

[61] Song Y H, Song N Y, Shin S Y, Kim H J, Yun D J, Lim C O, Lee S Y, Kang K Y, Hong J C. Isolation of CONSTANS as a TGA4/OBF4 interacting protein. Molecules and Cells, 2008, 25(4): 559-565.

[62] Muthreich M. Characterization of clade Ⅰ TGA transcription factors in Arabidopsis thaliana with respect to biotic stress [D]. Göttingen: der Georg-August-Universität zu Göttingen. 2014.

[63] Alvarez J M, Riveras E, Vidal E A, Gras D E, Contreras-Lopez O, Tamayo K P, Aceituno F, Gomez I, Ruffel S, Lejay L, Jordana X, Gutierrez R A. Systems approach identifies TGA1 and TGA4 transcription factors as important regulatory components of the nitrate response of Arabidopsis thaliana roots. The Plant Journal, 2014, 80(1): 1-13.

[64] Zhong L, Chen D, Min D, Li W, Xu Z, Zhou Y, Li L, Chen L, Chen M, M Y. AtTGA4, a bZIP transcription factor, confers drought resistance by enhancing nitrate transport and assimilation in Arabidopsis thaliana. Biochemical and Biophysical Research Communications, 2015, 457(3): 433-439.

[65] Farinati S, DalCorso G, Varotto S, Furini A. The Brassica juncea BjCdR15, an ortholog of Arabidopsis TGA3, is a regulator of cadmium uptake, transport and accumulation in shoots and confers cadmium tolerance in transgenic plants. New Phytologist, 2010, 185(4): 964-978.

[66] Qin X F, Holuigue L, Horvath D M, Chua N H. Immediate early transcription activation by salicylic acid via the cauliflower mosaic virus as-1 element. The Plant Cell, 1994, 6(6): 863-874.

[67] Jupin I, Chua N H. Activation of the CaMV as-1 cis-element by salicylic acid: Differential DNA-binding of a factor related to TGA1a. The EMBO Journal, 1996, 15(20): 5679.

[68] Kang H G, Klessig D F. Salicylic acid-inducible Arabidopsis CK2-like activity phosphorylates TGA2. Plant Molecular Biology, 2005, 57(4): 541-557.

[69] Singh R, Lee M O, Lee J E, Choi J, Park J H, Kim E H, Yoo R H. Rice mitogen-activated protein kinase interactom analysis using the yeast two-hybrid system. Plant Physiology, 2012, 160(1): 477-487.

[70] Fobert P R, Després C. Redox control of systemic acquired resistance. Current Opinion in Plant Biology, 2005, 8(4): 378-382.

[71] Delledonne M, Xia Y, Dixon R A, Lamb C. Nitric oxide functions as a signal in plant disease resistance. Nature, 1998, 394(6693): 585-588.

[72] Hong J K, Yun B W, Kang J G, Raja M U, Kwon E, Sorhagen K, Chu C, Wang Y, Loake C. Nitric oxide function and signalling in plant disease resistance. Journal of Experimental Botany, 2008, 59(2): 147-154.

[73] Bellin D, Asai S, Delledonne M, Yoshioka H. Nitric oxide as a mediator for defense responses. Molecular Plant-Microbe Interactions, 2013, 26(3): 271-277.

[74] Li S, Gutsche N, Zachgo S. The ROXY1 C-terminal L**LL motif is essential for the interaction with TGA. Plant Physiology, 2011, 157(4): 2056-2068.

[75] Zander M, Chen S, Imkampe J, Thurow C, Gatz C. Repression of the Arabidopsis thaliana jasmonic acid/ethylene-induced defense pathway by TGA-interacting glutaredoxins depends on their C-terminal ALWL motif. Molecular Plant, 2012, 5(4): 831-840.

[76] Gutsche N, Thurow C, Zachgo S, Gatz C. Plant-specific CC-type glutaredoxins: Functions in developmental processes and stress responses. Biological Chemistry, 2015, 396(5): 495-509.

[77] Szemenyei H, Hannon M, Long J A. TOPLESS mediates auxin- dependent transcriptional repression during Arabidopsis embryogenesis. Science, 2008, 319(5868): 1384-1386.

[78] Arabidopsis interactome mapping consortium. Evidence for network evolution in an Arabidopsis interactome map. Science, 2011,333: 601-607.

[79] Causier B, Ashworth M, Guo W, Davies B. The TOPLESS interactome: A framework for gene repression in Arabidopsis. Plant Physiology, 2012, 158(1): 423-438.

[80] Fitzgerald H A, Canlas P E, Chern M S, Ronald P C. Alteration of TGA factor activity in rice results in enhanced tolerance to Xanthomonas oryzae pv. oryzae. The Plant Journal, 2005, 43(3): 335-347.

[81] Bonasera J M, Kim J F, Beer S V. PR genes of apple: Identification and expression in response to elicitors and inoculation with Erwinia amylovora. BMC Plant Biology, 2006, 6(23): 1-12.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Progress on TGA Transcription Factors in Plant

RichHTML

PDF

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	ZHANG XiaoLi, TAO Wei, GAO GuoQing, CHEN Lei, GUO Hui, ZHANG Hua, TANG MaoYan, LIANG TianFeng. Effects of Direct Seeding Cultivation Method on Growth Stage, Lodging Resistance and Yield Benefit of Double-Cropping Early Rice [J]. Scientia Agricultura Sinica, 2023, 56(2): 249-263.
[2]	WU Yan,ZHANG Hao,LIANG ZhenHua,PAN AiLuan,SHEN Jie,PU YueJin,HUANG Tao,PI JinSong,DU JinPing. circ-13267 Regulates Egg Duck Granulosa Cells Apoptosis Through Let-7-19/ERBB4 Pathway [J]. Scientia Agricultura Sinica, 2022, 55(8): 1657-1666.
[3]	LIU Jiao,LIU Chang,CHEN Jin,WANG MianZhi,XIONG WenGuang,ZENG ZhenLing. Distribution Characteristics of Prophage in Multidrug Resistant Escherichia coli as well as Its Induction and Isolation [J]. Scientia Agricultura Sinica, 2022, 55(7): 1469-1478.
[4]	GUO ZeXi,SUN DaYun,QU JunJie,PAN FengYing,LIU LuLu,YIN Ling. The Role of Chalcone Synthase Gene in Grape Resistance to Gray Mold and Downy Mildew [J]. Scientia Agricultura Sinica, 2022, 55(6): 1139-1148.
[5]	YAN LeLe,BU LuLu,NIU Liang,ZENG WenFang,LU ZhenHua,CUI GuoChao,MIAO YuLe,PAN Lei,WANG ZhiQiang. Widely Targeted Metabolomics Analysis of the Effects of Myzus persicae Feeding on Prunus persica Secondary Metabolites [J]. Scientia Agricultura Sinica, 2022, 55(6): 1149-1158.
[6]	LI ShiJia,LÜ ZiJing,ZHAO Jin. Identification of R2R3-MYB Subfamily in Chinese Jujube and Their Expression Pattern During the Fruit Development [J]. Scientia Agricultura Sinica, 2022, 55(6): 1199-1212.
[7]	WANG Kai,ZHANG HaiLiang,DONG YiXin,CHEN ShaoKan,GUO Gang,LIU Lin,WANG YaChun. Definition and Genetic Parameters Estimation for Health Traits by Using on-Farm Management Data in Dairy Cattle [J]. Scientia Agricultura Sinica, 2022, 55(6): 1227-1240.
[8]	ZHANG YaLing, GAO Qing, ZHAO Yuhan, LIU Rui, FU Zhongju, LI Xue, SUN Yujia, JIN XueHui. Evaluation of Rice Blast Resistance and Genetic Structure Analysis of Rice Germplasm in Heilongjiang Province [J]. Scientia Agricultura Sinica, 2022, 55(4): 625-640.
[9]	JIA GuanQing, DIAO XianMin. Current Status and Perspectives of Innovation Studies Related to Foxtail Millet Seed Industry in China [J]. Scientia Agricultura Sinica, 2022, 55(4): 653-665.
[10]	WANG MengRui, LIU ShuMei, HOU LiXia, WANG ShiHui, LÜ HongJun, SU XiaoMei. Development of Artificial Inoculation Methodology for Evaluation of Resistance to Fusarium Crown and Root Rot and Screening of Resistance Sources in Tomato [J]. Scientia Agricultura Sinica, 2022, 55(4): 707-718.
[11]	XIANG MiaoLian, WU Fan, LI ShuCheng, WANG YinBao, XIAO LiuHua, PENG WenWen, CHEN JinYin, CHEN Ming. Effects of Melatonin Treatment on Resistance to Black Spot and Postharvest Storage Quality of Pear Fruit [J]. Scientia Agricultura Sinica, 2022, 55(4): 785-795.
[12]	HU ChaoYue, WANG FengTao, LANG XiaoWei, FENG Jing, LI JunKai, LIN RuiMing, YAO XiaoBo. Resistance Analyses on Wheat Stripe Rust Resistance Genes to the Predominant Races of Puccinia striiformis f. sp. tritici in China [J]. Scientia Agricultura Sinica, 2022, 55(3): 491-502.
[13]	TANG ZiYun,HU JianXin,CHEN Jin,LU YiXing,KONG LingLi,DIAO Lu,ZHANG FaFu,XIONG WenGuang,ZENG ZhenLing. Relationship Between Biofilm Formation and Molecular Typing of Staphylococcus aureus from Animal Origin [J]. Scientia Agricultura Sinica, 2022, 55(3): 602-612.
[14]	LI ZhiLing,LI XiangJu,CUI HaiLan,YU HaiYan,CHEN JingChao. Development and Application of ELISA Kit for Detection of EPSPS in Eleusine indica [J]. Scientia Agricultura Sinica, 2022, 55(24): 4851-4862.
[15]	YOU YuWan,ZHANG Yu,SUN JiaYi,ZHANG Wei. Genome-Wide Identification of NAC Family and Screening of Its Members Related to Prickle Development in Rosa chinensis Old Blush [J]. Scientia Agricultura Sinica, 2022, 55(24): 4895-4911.