基于数字图像技术的冬油菜氮素营养诊断

doi:10.3864/j.issn.0578-1752.2015.19.010

Abstract

Abstract: 【Objective】 In order to provide a scientific basis for digital image processing technique in nitrogen nondestructive diagnosis of winter rapeseed, a field experiment was carried out to explore the feasibility of digital image processing technique in nitrogen nondestructive diagnosis, determine the best digital parameter and regression equation. 【Method】 A field experiment was conducted with different nitrogen application rates (0, 90, 180, 270 and 360 kg·hm^-2). The pictures of winter rapeseed canopy were obtained with a digital camera, meanwhile, the conventional nitrogen diagnosis parameters of aboveground biomass, nitrogen concentration in leaf and leaf chlorophyll content at six-leaf period, ten-leaf period, bud period and blooming period, were determined and the correlations were analyzed. 【Result】 The red color intensity (R), normalized redness intensity (NRI) and the ratio of greenness and blueness intensity (G/B) had significant inverse correlations with the conventional N diagnosis parameters. But green color intensity (G), blue color intensity (B), the ratio of greenness and redness intensity (G/R), the ratio of blueness and redness intensity (B/R), the normalized greenness intensity (NGI) and the normalized blueness intensity (NBI) showed a significant positive correlation. Compared with other digital index, the normalized redness intensity (NRI) showed a prominently and very prominently inverse relationship with conventional N parameters and the regression equation with N application rate, aboveground biomass, leaf N concentration, chlorophyll in leaf, N uptake and N nutrition index is y(t·hm^-2)=-8.003x+2.706, y(t·hm^-2)=-106.072x+38.200, y(g·kg^-1)=-692.99x+261.84, y(mg·g^-1)=-12.750x+5.665, y(kg·hm^-2)=-4087.416x+1414.274 and y=-27.198x+9.812, the correlation values (R²) is 0.917^**, 0.746^** and 0.953^** between measured and estimated leaf N concentration, chlorophyll in leaf and NNI, RMSE and RE were 0.821, 26.32%, 0.330, 28.57% and 0.228, 28.39%, respectively. 【Conclusion】Digital image processing technique can be used in nitrogen nondestructive diagnosis of winter rapeseed, the stage before bud period is crucial period with NRI as the index for the N nitrogen diagnosis using digital image processing technique, and NRI is the best diagnosis index with linear regression equation.

Key words: winter rapeseed, digital image, nitrogen, nutritional diagnosis, equation model

WEI Quan-quan, LI Lan-tao, REN Tao, WANG Zhen, WANG Shao-hua, LI Xiao-kun, CONG Ri-huan, LU Jian-wei. Diagnosing Nitrogen Nutrition Status of Winter Rapeseed via Digital Image Processing Technique[J].Scientia Agricultura Sinica, 2015, 48(19): 3877-3886.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2015.19.010

https://www.chinaagrisci.com/EN/Y2015/V48/I19/3877

References

[1] 刘后利. 实用油菜栽培学. 上海: 上海科技出版社, 1987: 243-244.

Liu H L. Applied Cultivation of Rapeseed. Shanghai: Shanghai Science and Technology Press, 1987: 243-244. (in Chinese)

[2] 李慧, 马常宝, 鲁剑巍, 李小坤, 任涛, 丛日环. 中国不同区域油菜氮磷钾肥的增产效果. 中国农业科学, 2013, 46(9): 1837-1847.

Li H, Ma C B, Lu J W, Li X K, Ren T, Cong R H. Increasing effect of N, P and K fertilizer on rapeseed in different regions of China. Scientia Agricultura Sinica, 2013, 46(9): 1837-1847. (in Chinese)

[3] 邹娟, 鲁剑巍, 陈防, 李银水, 李小坤. 冬油菜施氮的增产和养分吸收效应及氮肥利用率研究. 中国农业科学, 2011, 44(4): 745-752.

Zou J, Lu J W, Chen F, Li Y S, Li X K. Study on yield increasing and nutrient uptake effect by nitrogen application and nitrogen use efficiency for winter rapeseed. Scientia Agricultura Sinica, 2011, 44(4): 745-752. (in Chinese)

[4] 刘春增, 寇长林, 王秋杰. 长期施肥对砂土肥力变化及硝态氮积累和分布的影响. 土壤通报, 1996, 27(5): 216-218.

Liu C Z, Kou C L, Wang Q J. Effects of long-term fertilization on sand soil fertility change and the accumulation and distribution of nitrate nitrogen. Chinese Journal of Soil Science, 1996, 27(5): 216-218. (in Chinese)

[5] 张玉良. 农业化学与生物圈. 北京: 中国环境科学出版社, 1987: 151-175.

Zhang Y L. Agricultural Chemistry and Biosphere. Beijing: China Environmental Science Press, 1987: 151-175. (in Chinese)

[6] 喻歌农, 周泳. 试论精准农业及我国行动对策. 自然资源学报, 1999, 14(1): 69-74.

Yu G N, Zhou Y. Precision agriculture and its prospective in China. Journal of Natural Resources, 1999, 14(1): 69-74. (in Chinese)

[7] 贾良良, 范明生, 张福锁, 陈新平, 吕世华, 孙彦铭. 应用数码相机进行水稻氮素营养诊断. 光谱学与光谱分析, 2009, 29(8): 2176-2179.

Jia L L, Fan M S, Zhang F S, Chen X P, Lü S H, Sun Y M. Nitrogen status diagnosis of rice by using a digital camera. Spectroscopy and Spectral Analysis, 2009, 29(8): 2176-2179. (in Chinese)

[8] 李刚华, 薛利红, 尤娟, 王绍华, 丁艳锋, 吴昊, 杨文祥. 水稻氮素和叶绿素SPAD叶位分布特点及氮素诊断的叶位选择. 中国农业科学, 2007, 40(6): 1127-1134.

Li G H, Xue L H, You J, Wang S H, Ding Y F, Wu H, Yang W X. Spatial distribution of leaf N content and SPAD value and determination of the suitable leaf for N diagnosis in rice. Scientia Agricultura Sinica, 2007, 40(6): 1127-1134. (in Chinese)

[9] 李岚涛, 刘波, 鲁剑巍, 任涛, 李小坤, 丛日环, 吴礼树, 林迪. 冬油菜叶片SPAD的时空分布和氮素诊断的叶位选择. 中国油料作物学报, 2014, 36(1): 76-83.

Li L T, Liu B, Lu J W, Ren T, Li X K, Cong R H, Wu L S, Lin D. Spatial distribution of SPAD value and determination of the suitable leaf for N diagnosis in winter rapeseed. Chinese Journal of Oil Crop Sciences, 2014, 36(1): 76-83. (in Chinese)

[10] Blackmer T M, Schepers J S. Use of a chlorophyll meter to monitor nitrogen status and schedule fertigation for corn. Journal of Production Agriculture, 1995, 8(1): 56-60.

[11] 赵满兴, 周建斌, 翟丙年, 杨绒, 李生秀. 旱地不同冬小麦品种氮素营养的叶绿素诊断. 植物营养与肥料学报, 2005, 11(4): 461-466.

Zhao M X, Zhou J B, Zhai B N, Yang R, Li S X. Chlorophyll diagnoses of N nutrition for different winter wheat cultivars on dryland. Plant Nutrition and Fertilizer Science, 2005, 11(4): 461-466. (in Chinese)

[12] Lin F F, Qiu L F, Deng J S, Shi Y Y, Chen L S, Wang K. Investigation of SPAD meter-based indices for estimating rice nitrogen status. Computers and Electronics in Agriculture, 2010, 71(Suppl.1): S60-S65.

[13] 贾良良. 应用数字图像技术与土壤植株测试进行冬小麦氮营养诊断[D]. 北京: 中国农业大学, 2003.

Jia L L. To detect nitrogen status of winter wheat by using color digital camera[D]. Beijing: China Agricultural University, 2003. (in Chinese)

[14] Ali M M, Al-Ani A, Eamus D, Tan D K Y. A new image processing based technique to determine chlorophyll in plants. American- Eurasian Journal of Agricultural & Environmental Sciences, 2012, 12(10): 1323-1328.

[15] Wang F, Haruhiko Y, Yasuomi I. Measuring leaf necrosis and chlorosis of bamboo induced by typhoon 0613 with RGB image analysis. Journal of Forestry Research, 2008, 19(3): 225-230.

[16] 王远, 王德建, 张刚, 王灿. 基于数码相机的水稻冠层图像分割及氮素营养诊断. 农业工程学报, 2012, 28(17): 131-136.

Wang Y, Wang D J, Zhang G, Wang C. Digital camera-based image segmentation of rice canopy and diagnosis of nitrogen nutrition. Transactions of the Chinese Society of Agricultural Engineering, 2012, 28(17): 131-136. (in Chinese)

[17] Rorie R L, Purcell L C, Mozaffari M, Karcher D E, King A, Marsh M C. Association of “greenness” in corn with yield and leaf nitrogen concentration. Agronomy Journal, 2011, 103(2): 529-535.

[18] Jia L L, Chen X P, Zhang F S, Bürkert A, Römheld V. Use of digital camera to assess nitrogen status of winter wheat in the Northern China Plain. Journal of Plant Nutrition, 2004, 27(3): 441-450.

[19] 刘绚霞, 董振生, 刘创社, 董军刚, 李红兵. 油菜叶绿素提取方法的研究. 中国农学通报, 2004, 20(4): 62-63.

Liu X X, Dong Z S, Liu C S, Dong J G, Li H B. Study on extracting methods of chlorophyll in rape. Chinese Agricultural Science Bulletin, 2004, 20(4): 62-63. (in Chinese)

[20] 鲍士旦. 土壤农化分析. 3版. 北京: 中国农业出版社, 2000.

Bao S D. Soil and Agro-chemistry Analysis. 3rd ed. Beijing: China Agriculture Press, 2000. (in Chinese)

[21] 李岚涛, 张萌, 任涛, 李小坤, 丛日环, 吴礼树, 鲁剑巍. 应用数字图像技术进行水稻氮素营养诊断. 植物营养与肥料学报, 2015, 21(1): 259-268.

Li L T, Zhang M, Ren T, Li X K, Cong R H, Wu L S, Lu J W. Diagnosis of N nutrition of rice using digital image processing technique. Journal of Plant Nutrition and Fertilizer, 2015, 21(1): 259-268. (in Chinese)

[22] Lemaire G, Gastal F, Salette J. N Uptake and Distribution in Plant Canopies: Diagnosis of the Nitrogen Status in Crops. Berlin: Springer,1997.

[23] Justes E, Mary B, Meynard J M, Machet J M, Thelier-Huche L. Determination of a critical nitrogen dilution curve for winter wheat crops. Annals of Botany, 1994, 74: 397-407.

[24] Colnenne C, Meynard J M, Reau R, Justes E, Merrien A. Determination of a critical nitrogen dilution curve for winter oilseed rape. Annals of Botany, 1998, 81: 311-317.

[25] 扈立家, 李天来. 我国发展精准农业的问题及对策. 沈阳农业大学学报: 社会科学版, 2005, 7(4): 400-402.

Hu L J, Li T L. Problems and Solutions of the development of precision agriculture of China. Journal of Shenyang Agricultural University: Social Sciences Edition, 2005, 7(4): 400-402. (in Chinese)

[26] Swain K C, Thomson S J, Jayasuriya H P W. Adoption of an unmanned helicopter for low-altitude remote sensing to estimate yield and total biomass of a rice crop. Transactions of the ASABE, 2010, 53(1): 21-27.

[27] 祝锦霞, 陈祝炉, 石媛媛, 王珂, 邓劲松. 基于无人机和地面数字影像的水稻氮素营养诊断研究. 浙江大学学报: 农业与生命科学版, 2010, 36(1): 78-83.

Zhu J X, Chen Z L, Shi Y Y, Wang K, Deng J S. Diagnoses of rice nitrogen status based on spectral characteristics of leaf and canopy. Journal of Zhejiang University: Agriculture & Life Sciences, 2010, 36(1): 78-83. (in Chinese)

Related Articles 15

[1]	YAN YanGe, ZHANG ShuiQin, LI YanTing, ZHAO BingQiang, YUAN Liang. Effects of Dextran Modified Urea on Winter Wheat Yield and Fate of Nitrogen Fertilizer [J]. Scientia Agricultura Sinica, 2023, 56(2): 287-299.
[2]	XU JiuKai, YUAN Liang, WEN YanChen, ZHANG ShuiQin, LI YanTing, LI HaiYan, ZHAO BingQiang. Nitrogen Fertilizer Replacement Value of Livestock Manure in the Winter Wheat Growing Season [J]. Scientia Agricultura Sinica, 2023, 56(2): 300-313.
[3]	ZHAO HaiXuan,ZHANG YiTao,LI WenChao,MA WenQi,ZHAI LiMei,JU XueHai,CHEN HanTing,KANG Rui,SUN ZhiMei,XI Bin,LIU HongBin. Spatial Characteristic and Its Factors of Nitrogen Surplus of Crop and Livestock Production in the Core Area of the Baiyangdian Basin [J]. Scientia Agricultura Sinica, 2023, 56(1): 118-128.
[4]	XIONG WeiYi,XU KaiWei,LIU MingPeng,XIAO Hua,PEI LiZhen,PENG DanDan,CHEN YuanXue. Effects of Different Nitrogen Application Levels on Photosynthetic Characteristics, Nitrogen Use Efficiency and Yield of Spring Maize in Sichuan Province [J]. Scientia Agricultura Sinica, 2022, 55(9): 1735-1748.
[5]	HOU JiangJiang,WANG JinZhou,SUN Ping,ZHU WenYan,XU Jing,LU ChangAi. Spatiotemporal Patterns in Nitrogen Response Efficiency of Aboveground Productivity Across China’s Grasslands [J]. Scientia Agricultura Sinica, 2022, 55(9): 1811-1821.
[6]	SANG ShiFei,CAO MengYu,WANG YaNan,WANG JunYi,SUN XiaoHan,ZHANG WenLing,JI ShengDong. Research Progress of Nitrogen Efficiency Related Genes in Rice [J]. Scientia Agricultura Sinica, 2022, 55(8): 1479-1491.
[7]	WU Yue,SUI XinHua,DAI LiangXiang,ZHENG YongMei,ZHANG ZhiMeng,TIAN YunYun,YU TianYi,SUN XueWu,SUN QiQi,MA DengChao,WU ZhengFeng. Research Advances of Bradyrhizobia and Its Symbiotic Mechanisms with Peanut [J]. Scientia Agricultura Sinica, 2022, 55(8): 1518-1528.
[8]	GUI RunFei,WANG ZaiMan,PAN ShengGang,ZHANG MingHua,TANG XiangRu,MO ZhaoWen. Effects of Nitrogen-Reducing Side Deep Application of Liquid Fertilizer at Tillering Stage on Yield and Nitrogen Utilization of Fragrant Rice [J]. Scientia Agricultura Sinica, 2022, 55(8): 1529-1545.
[9]	GAO JiaRui,FANG ShengZhi,ZHANG YuLing,AN Jing,YU Na,ZOU HongTao. Characteristics of Organic Nitrogen Mineralization in Paddy Soil with Different Reclamation Years in Black Soil of Northeast China [J]. Scientia Agricultura Sinica, 2022, 55(8): 1579-1588.
[10]	WANG Miao,ZHANG Yu,LI RuiQiang,XIN XiaoPing,ZHU XiaoYu,CAO Juan,ZHOU ZhongYi,YAN RuiRui. Effects of Grazing Disturbance on the Stoichiometry of Nitrogen and Phosphorus in Plant Organs of Leymus chinensis Meadow Steppe [J]. Scientia Agricultura Sinica, 2022, 55(7): 1371-1384.
[11]	YU QiLong,HAN YingYan,HAO JingHong,QIN XiaoXiao,LIU ChaoJie,FAN ShuangXi. Effect of Exogenous Spermidine on Nitrogen Metabolism of Lettuce Under High-Temperature Stress [J]. Scientia Agricultura Sinica, 2022, 55(7): 1399-1410.
[12]	CHAO ChengSheng,WANG YuQian,SHEN XinJie,DAI Jing,GU ChiMing,LI YinShui,XIE LiHua,HU XiaoJia,QIN Lu,LIAO Xing. Characteristics of Efficient Nitrogen Uptake and Transport of Rapeseed at Seedling Stage [J]. Scientia Agricultura Sinica, 2022, 55(6): 1172-1188.
[13]	LIU Miao,LIU PengZhao,SHI ZuJiao,WANG XiaoLi,WANG Rui,LI Jun. Critical Nitrogen Dilution Curve and Nitrogen Nutrition Diagnosis of Summer Maize Under Different Nitrogen and Phosphorus Application Rates [J]. Scientia Agricultura Sinica, 2022, 55(5): 932-947.
[14]	ZHANG XueLin, WU Mei, HE TangQing, ZHANG ChenXi, TIAN MingHui, LI XiaoLi, HOU XiaoPan, HAO XiaoFeng, YANG QingHua, LI ChaoHai. Effects of Crop Residue Decomposition on Soil Inorganic Nitrogen and Greenhouse Gas Emissions from Fluvo-Aquic Soil and Shajiang Black Soil [J]. Scientia Agricultura Sinica, 2022, 55(4): 729-742.
[15]	SHI Xi, NING LiHua, GE Min, WU Qi, ZHAO Han. Screening and Application of Biomarkers Related to Maize Nitrogen Status [J]. Scientia Agricultura Sinica, 2022, 55(3): 438-450.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Diagnosing Nitrogen Nutrition Status of Winter Rapeseed via Digital Image Processing Technique

RichHTML

PDF

Cited