外源精胺对菜用大豆贮藏冷害及蔗糖代谢的影响

doi:10.3864/j.issn.0578-1752.2015.08.13

Abstract

Abstract: 【Objective】 Mechanism of exogenous spermine in delaying chilling injury and regulating sucrose metabolism of vegetable soybean during cold storage was investigated to provide a theoretical basis for the preservation of post-harvest vegetable soybean. 【Method】 ‘Xindali No. 1’ vegetable soybeans used as the experimental materials were immersed in 0.5, 1 and 2 mmol·L^-1 spermine for 20 min, respectively, distilled water as a control, then stored at 1℃ for 10 weeks under the environmental condition of relative humidity of 85%-90%. The chilling resistance related physiological indexes, soluble sugars (sucrose, fructose and glucose) contents and the activities of sucrose metabolism related enzymes were all determined every week.【Result】 The chilling injury (CI) symptoms of vegetable soybean aggravated with the extension of storage period, and it appeared during the first two weeks of storage. Spermine treatment not only delayed the increase of CI index and postponed the symptoms until the 3rd week, but also inhibited cell membrane permeability, especially after 5 weeks of storage, both 0.5 and 1 mmol·L^-1 spermine treatment groups reached significant levels (P<0.05) compared with the control group. In addition, during cold storage, the membrane lipid peroxidation product, including malondialdehyde (MDA) gradually accumulated in vegetable soybean, low concentration of spermine could reduce the lipid peroxidation and decrease MDA accumulation, while high concentration of spermine had a toxic effect. Compared with the control group, 1 mmol·L^-1 spermine significantly improved the activities of antioxidant enzymes (CAT, POD and SOD) of vegetable soybeans during storage. And meanwhile, spermine treatment also significantly (P<0.05) reduced the sucrose loss of vegetable soybean, fructose and glucose contents in spermine-treated vegetable soybean grains were lower than the control group, but the concentration differences among all treatment groups were not significant (P>0.05) during the whole storage period. Through the correlation analysis between soluble sugars contents and sucrose metabolism related enzyme activities in vegetable soybean grains during cold storage, it indicated that sucrose content had a significant negative correlation (P<0.01) with AI activity, but a significant positive correlation (P<0.01) with SPS activity, and no significant correlation with NI and SS activities, which meant AI and SPS played a key role in sucrose degradation of vegetable soybean during cold storage, exogenous spermine effectively inhibited the sucrose loss by regulating the activities of sucrose-metabolizing enzymes in vegetable soybean.【Conclusion】 Spermine effectively delayed the chilling injury of vegetable soybean during storage at 1℃, and a dose of 1 mmol·L^-1 could be an effective treatment. Sucrose loss might cause the aggravated chilling injury, while spermine maintained higher sucrose content by simultaneously inhibiting the increase of AI activity and the decrease of SPS activity in vegetable soybean during cold storage.

Key words: vegetable soybean, spermine, storage, chilling injury, sucrose metabolism

LIU Chun-quan, SONG Jiang-feng, WANG Yuan, LI Da-jing. Effect of Exogenous Spermine on Chilling Injury and Sucrose Metabolism of Post-Harvest Vegetable Soybean[J].Scientia Agricultura Sinica, 2015, 48(8): 1588-1596.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2015.08.13

https://www.chinaagrisci.com/EN/Y2015/V48/I8/1588

References

[1] 王丹英, 汪自强, 方勇, 徐律平. 菜用大豆食味品质及其与内含物关系研究. 金华职业技术学院学报, 2002(3): 15-17.

Wang D Y, Wang Z Q, Fang Y. Studies on the relationship between vegetable soybean eating quality and its components. Journal of Jinhua College of Profession and Technology, 2002(3): 15-17. (in Chinese)

[2] 王远, 宋江峰, 刘春泉, 李大婧. 不同贮藏温度条件下菜用大豆蔗糖代谢与相关酶活性变化. 食品科学, 2014, 35(18): 185-189.

Wang Y, Song J F, Liu C Q, Li D J. Changes of sucrose metabolism and related enzyme activities in vegetable soybean at different storage temperatures. Food Science, 2014, 35(18): 185-189. (in Chinese)

[3] Raina A, Jänne J. Physiology of the natural polyamines putrescine, spermidine and spermine. Medical Biology, 1975, 53(3): 121-147.

[4] Choudhary S P, Oral H V, Bhardwaj R, Bhardwaj R, Yu J Q, Tran L S P. Interaction of brassinosteroids and polyamines enhances copper stress tolerance in Raphanus sativus. Journal of Experimental Botany, 2012, 63(15): 5659-5675.

[5] Asghari M R, Abdollahi R. Changes in quality of strawberries during cold storage in response to postharvest nitric oxide and putrescine treatments. Acta Alimentaria, 2013, 42(4): 529-539.

[6] Barman K, Asrey R, Pal R K. Putrescine and carnauba wax pretreatments alleviate chilling injury, enhance shelf life and preserve pomegranate fruit quality during cold storage. Scientia Horticulturae, 2011, 130(4): 795-800.

[7] Champa W A, Gill M I S, Mahajan B V C, Bedi S. Postharvest treatment of polyamines maintains quality and extends shelf-life of table grapes (Vitis vinifera L.) cv. flame seedless. Postharvest Biology and Technology, 2014, 91: 57-63.

[8] Bal E. Effects of exogenous polyamine and ultrasound treatment to improve peach storability. Chilean Journal of Agricultural Research, 2013, 73(4): 435-440.

[9] Cao S F, Yang Z F, Zheng Y H. Sugar metabolism in relation to chilling tolerance of loquat fruit. Food Chemistry, 2013, 136(1): 139-143.

[10] Wang K, Shao X F, Gong Y F, Zhu Y, Wang H F, Zhang H L, Yu D D, Yu F, Qiu Z Y, Lu H. The metabolism of soluble carbohydrates related to chilling injury in peach fruit exposed to cold stress. Postharvest Biology and Technology, 2013, 86: 53-61.

[11] 贾永霞. 外源亚精胺提高黄瓜幼苗低氧胁迫耐性的生理调节功能 [D]. 南京: 南京农业大学, 2009.

Jia Y X. studies on physiological regulation function of exogenous spermidine on cucumber seedlings tolerance to hypoxia [D]. Nanjing: Nanjing Agricultural University, 2009. (in Chinese)

[12] Kassinee S, Matsui T, Okuda N. Changes in acid invertase activity and sugar distribution during postharvest senescence in vegetable soybean. Asian Journal of Plant Sciences, 2004, 3(4): 433-438.

[13] Sugimoto M, Goto H, Otomo K, Ito M, Onuma H, Sugaware M, Abe S, Tomita M, Soga T. Metabolomic profiles and sensory attributes of edamame under various storage duration and temperature conditions. Journal of Agricultural and Food Chemistry, 2010, 58(14): 8418-8425.

[14] Saldivar X, Wang Y J, Chen P, Mauromoustakos A. Effects of blanching and storage conditions on soluble sugar contents in vegetable soybean. LWT-Food Science and Technology, 2010, 43(9): 1368-1372.

[15] 张平, 张鹏, 刘辉, 陈绍慧, 张秀敏, 李江阔. 不同低温处理对樱桃冷害发生的影响. 食品科学, 2012, 33(12): 303-308.

Zhang P, Zhang P, Liu H, Chen S H, Zhang X M, Li J K. Effect of low-temperature treatment on chilling injury in cherry fruits. Food Science, 2012, 33(12): 303-308. (in Chinese)

[16] 胡位荣, 张昭其, 季作樑, 刘顺枝. 冷害对荔枝果皮膜脂过氧化和保护酶活性的影响. 华南农业大学学报, 2004, 25(3): 6-9.

Hu W R, Zhang Z Q, Ji Z L, Liu S Z. Effect of chilling injury on membrane lipid peroxidation and activities of cell defense enzyme in litchi pericarp. Journal of South China Agricultural University, 2004, 25(3): 6-9. (in Chinese)

[17] Razzaq K, Khan A S, Malik A U, Shahid M, Ullah S. Role of putrescine in regulating fruit softening and antioxidative enzyme systems in ‘Samar Bahisht Chaunsa’mango. Postharvest Biology and Technology, 2014, 96: 23-32.

[18] Nielsen T H, Skjærbæ H C, Karlsen P. Carbohydrate metabolism during fruit development in sweet pepper (Capsicum annuum) plants. Physiologia Plantarum, 1991, 82(2): 311-319.

[19] Sayyari M, Babalar M, Kalantari S, Romero D M, Guillén F, Serrano M, Valero D. Vapour treatments with methyl salicylate or methyl jasmonate alleviated chilling injury and enhanced antioxidant potential during postharvest storage of pomegranates. Food Chemistry, 2011, 124(3): 964-970.

[20] Champa W A, Gill M I S, Mahajan B V C, Bedi S. Exogenous treatment of spermine to maintain quality and extend postharvest life of table grapes (Vitis vinifera L.) cv. Flame Seedless under low temperature storage. LWT-Food Science and Technology, 2015, 60(1): 412-419.

[21] 刘宾, 王学君, 毛江胜, 邓立刚, 王雪, 赵平娟, 王文正, 李桂凤. 精胺处理对凯特杏低温贮藏期间生理特性的影响. 食品科学, 2010(22): 358-360.

Liu B, Wang X G, Mao J S, Deng L G, Wang X, Zhao P J, Wang Z W, Li G Y. Effect of spermine treatment on physiological characteristics of Katy fruits (Prunus armeniaca L.) during low temperature storage. Food Science, 2010(22): 358-360. (in Chinese)

[22] 王学. 低温胁迫下精胺对黄瓜幼苗抗氧化酶系统及膜脂过氧化的影响. 种子, 2008, 27(11): 33-36.

Wang X. Effects of spermine on antioxidant enzyme systems and membrane lipid peroxidation in cucumber seedling under low temperature stress. Seeds, 2008, 27(11): 33-36. (in Chinese)

[23] Gill S S, Tuteja N. Polyamines and abiotic stress tolerance in plants. Plant Signaling & Behavior, 2010, 5(1): 26-33.

[24] 陈克明, 陈伟, 杨震峰. 桃果实采后可溶性糖和果胶类物质的变化与低温冷害的关系. 核农学报, 2013, 27(5): 647-652.

Chen K M, Chen W, Yang Z F. The relationship between the changes of soluble sugar and pectic substances in peach fruit after harvest and chilling injury. Journal of Nuclear Agricultural Sciences, 2013, 27(5): 647-652. (in Chinese)

[25] Shao X F, Zhu Y, Cao S F, Wang H F, Song Y X. Soluble sugar content and metabolism as related to the heat-induced chilling tolerance of loquat fruit during cold storage. Food and Bioprocess Technology, 2013, 6(12): 3490-3498.

[26] Purvis A C, Grierson W. Accumulation of reducing sugar and resistance of grapefruit peel to chilling injury as related to winter temperatures. Journal of American Society for Horticultural Science, 1982, 107(1): 139-142.

[27] Zanor M I, Osorio S, Nunes N A, Carrsri F, Loshe M, Usadel B, Kühn C, Bleiss W, Giavalisco P, Willmitzer L, Sulpice R, Zhou Y H, Fernie A R. RNA interference of LIN5 in tomato confirms its role in controlling Brix content, uncovers the influence of sugars on the levels of fruit hormones, and demonstrates the importance of sucrose cleavage for normal fruit development and fertility. Plant Physiology, 2009, 150(3): 1204-1218.

[28] 潘秋红, 张大鹏. 植物酸性转化酶基因及其表达调控. 植物学通报, 2005, 22(2): 129-137.

Pan Q H, Zhang D P. Plant acid invertase gene and regulation of its expression. Chinese Bulletin of Botany, 2005, 22(2):129-137. (in Chinese)

[29] Sunkar R, Li Y F, Jagadeeswaran G. Functions of microRNAs in plant stress responses. Trends in Plant Science, 2012, 17(4): 196-203.

[30] Mao L, Que F, Wang G. Sugar metabolism and involvement of enzymes in sugarcane (Saccharum officinarum L.) stems during storage. Food Chemistry, 2006, 98(2): 338-342.

[31] Choudhury S R, Roy S, Das R, Sengupta D N. Differential transcriptional regulation of banana sucrose phosphate synthase gene in response to ethylene, auxin, wounding, low temperature and different photoperiods during fruit ripening and functional analysis of banana SPS gene promoter. Planta, 2008, 229(1): 207-223.

[32] Itai A, Tanahahi T. Inhibition of sucrose loss during cold storage in Japanese pear (Pyrus pyrifolia Nakai) by 1-MCP. Postharvest Biology and Technology, 2008, 48(3): 355-363.

[33] Zhu Z, Liu R, Li B, Tian S P. Characterisation of genes encoding key enzymes involved in sugar metabolism of apple fruit in controlled atmosphere storage. Food Chemistry, 2013, 141(4): 3323-3328.

Related Articles 15

[1]	XIE YiTong,ZHANG Fei,SHI Jie,FENG Li,JIANG Li. Effects of Exogenous Sucrose on the Postharvest Quality and Chloroplast of Gynura bicolor D.C [J]. Scientia Agricultura Sinica, 2022, 55(8): 1642-1656.
[2]	LÜ XinNing,WANG Yue,JIA RunPu,WANG ShengNan,YAO YuXin. Effects of Melatonin Treatment on Quality of Stored Shine Muscat Grapes Under Different Storage Temperatures [J]. Scientia Agricultura Sinica, 2022, 55(7): 1411-1422.
[3]	PENG Xue,GAO YueXia,ZHANG LinXuan,GAO ZhiQiang,REN YaMei. Effects of High-Energy Electron Beam Irradiation on Potato Storage Quality and Bud Eye Cell Ultrastructure [J]. Scientia Agricultura Sinica, 2022, 55(7): 1423-1432.
[4]	DONG FuCheng,MA ShuLi,SHI JuanJuan,ZHANG JunMei,CUI Yan,REN YouShe,ZHANG ChunXiang. Expression and Localization of LCN5 in Ram Reproductive Organs and Spermatozoa [J]. Scientia Agricultura Sinica, 2022, 55(7): 1445-1457.
[5]	XIANG MiaoLian, WU Fan, LI ShuCheng, WANG YinBao, XIAO LiuHua, PENG WenWen, CHEN JinYin, CHEN Ming. Effects of Melatonin Treatment on Resistance to Black Spot and Postharvest Storage Quality of Pear Fruit [J]. Scientia Agricultura Sinica, 2022, 55(4): 785-795.
[6]	SONG JiangTao,SHEN DanDan,GONG XuChen,SHANG XiangMing,LI ChunLong,CAI YongXi,YUE JianPing,WANG ShuaiLing,ZHANG PuFen,XIE ZongZhou,LIU JiHong. Effects of Artificial Fruit Thinning on Sugar and Acid Content and Expression of Metabolism-Related Genes in Fruit of Beni-Madonna Tangor [J]. Scientia Agricultura Sinica, 2022, 55(23): 4688-4701.
[7]	JIN MengJiao,LIU Bo,WANG KangKang,ZHANG GuangZhong,QIAN WanQiang,WAN FangHao. Light Energy Utilization and Response of Chlorophyll Synthesis Under Different Light Intensities in Mikania micrantha [J]. Scientia Agricultura Sinica, 2022, 55(12): 2347-2359.
[8]	ZOU YunQian,LIN ZiZhen,XU RangWei,CHENG YunJiang. Development and Evaluation of a Coating Substitute for Individual Polyethylene Film Packaging of Citrus Fruit [J]. Scientia Agricultura Sinica, 2022, 55(12): 2398-2412.
[9]	HU FeiFei,QIAN ShuYi,HUANG Feng,JIANG Wei,QIANG Yu,JIANG Feng,HU HaiMei,LI Xia,ZHANG ChunHui. Effect of Low Voltage Electrostatic Field-Assisted Short-Term Frozen Storage on Quality of Pork [J]. Scientia Agricultura Sinica, 2021, 54(9): 1993-2005.
[10]	HAN ZhanYu,WU ChunYan,XU YanQiu,HUANG FuDeng,XIONG YiQin,GUAN XianYue,ZHOU LuJian,PAN Gang,CHENG FangMin. Effects of High-Temperature at Filling Stage on Grain Storage Protein Accumulation and Its Biosynthesis Metabolism for Rice Plants Under Different Nitrogen Application Levels [J]. Scientia Agricultura Sinica, 2021, 54(7): 1439-1454.
[11]	ZHENG FengJun, WANG Xue, LI ShengPing, LIU XiaoTong, LIU ZhiPing, LU JinJing, WU XuePing, XI JiLong, ZHANG JianCheng, LI YongShan. Synergistic Effects of Soil Moisture, Aggregate Stability and Organic Carbon Distribution on Wheat Yield Under No-Tillage Practice [J]. Scientia Agricultura Sinica, 2021, 54(3): 596-607.
[12]	LIU Lian,TANG ZhiPeng,LI FeiFei,XIONG Jiang,LÜ BiWen,MA XiaoChuan,TANG ChaoLan,LI ZeHang,ZHOU Tie,SHENG Ling,LU XiaoPeng. Fruit Quality in Storage, Storability and Peel Transcriptome Analysis of Rong’an Kumquat, Huapi Kumquat and Cuimi Kumquat [J]. Scientia Agricultura Sinica, 2021, 54(20): 4421-4433.
[13]	ZHAO FuMei,WANG Shuang,TIAN YuTing,QIAO Qi,WANG YongJiang,ZHANG DeSheng,ZHANG ZhenChen. An Investigation into Key Factors Influencing the Occurrence of Virus Disease in Sweet Potato [J]. Scientia Agricultura Sinica, 2021, 54(15): 3232-3240.
[14]	WANG HuiLing,YAN AiLing,SUN Lei,ZHANG GuoJun,WANG XiaoYue,REN JianCheng,XU HaiYing. Effects of Low Temperature Storage on Monoterpenes in Table Grape [J]. Scientia Agricultura Sinica, 2021, 54(1): 164-178.
[15]	GU MingHui,LIU YongFeng,SHEN Qian,QIAO ChunYan. Improving Quality and Delaying Oxidation in Goat Meat Refrigeration by Polyphenols from Thinned Young Kiwifruit [J]. Scientia Agricultura Sinica, 2020, 53(8): 1643-1654.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Effect of Exogenous Spermine on Chilling Injury and Sucrose Metabolism of Post-Harvest Vegetable Soybean

RichHTML

PDF

Cited