根系边缘细胞对肉桂酸胁迫下黄瓜和黑籽南瓜活性氧代谢与根系活力的影响

doi:10.3864/j.issn.0578-1752.2015.08.12

Abstract

Abstract: 【Objective】The aim of this paper was to clarify the defensive mechanism of root border cells (RBC) against cinnamic acid (CA) stress by analyzing the reactive oxygen metabolism and root activity of seedling RBC in cucumber (Cucumis sativus L.) and figleaf gourd(Cururbita ficifolia) under CA stress. 【Method】 The 5 mm length of aeroponicly cultured roots of cucumber cultivar Zhongnong No. 16 andfigleaf gourd cultivar bouche were divided into two groups: one group was used to investigate reactive oxygen metabolism by spraying with 0 and 0.25 mmol·L^-1 CA at 1 h interval, another group rinsing root tips of distilled water once every 4 h firstly and RBC were removed, then spraying 0 and 0.25 mmol·L^-1 CA every 1 h. The metabolic index of active oxygen was measured after treatment for 0, 12, 24 and 36 hours of CA stress, root fresh weight, root respiration rate and root activity of the seedlings were measured after 24 hours. 【Result】The root fresh weight and physiological metabolism were not affected significantly by RBC without CA stress. If the RBC were not removed, the root fresh weight and root activity decreased and the level of reactive oxygen species (ROS) and malonaldehyde (MDA), total respiration rate, CN-resistant respiration rate, the activities of superoxide dismutase (SOD), catalase (CAT) and guaiacol-dependent peroxidase (POD) increased significantly in cucumber under CA stress. However, the root fresh weight, root activity, the level of ROS and MDA, total respiration rate, CN-resistant respiration rate, the activities of SOD, CAT and POD were not affected significantly in figleaf gourd seedlings. If RBC were removed, the effect of CA stress on figleaf gourd was similar with cucumber, but significantly than RBC not removing. 【Conclusion】 RBC possibly attenuated the CA toxicity to the roots of cucumber and figleaf gourd through decreasing ROS and MDA content. The defensive ability of RBCs against CA on figleaf gourd was stronger than that of cucumber. Removal of RBCs could lead to great damage to the roots of cucumber and figleaf gourd significantly under CA stress.

Key words: cucumber, figleaf gourd, cinnamic acid, root border cells, reactive oxygen species

QIAO Yong-xu, ZHANG Yong-ping, GAO Li-hong. Effect of Root Border Cells on Reactive Oxygen Metabolism and Root Activity of Cucumber and Figleaf Gourd Seedlings Under Cinnamic Acid Stress[J].Scientia Agricultura Sinica, 2015, 48(8): 1579-1587.

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References

[1] Canalsa R M, Emeterioa L S, Peraltab J. Autotoxicity in Lolium rigidum: analyzing the role of chemically mediated interactions in annual plant populations. Journal of Theoretical Biology, 2005, 235: 402-407.

[2] 乔永旭, 张永平, 高丽红. 黄瓜和黑籽南瓜对肉桂酸及亚低温交叉胁迫的应答差异. 中国农业大学学报, 2014, 19(4): 86-94.

Qiao Y X, Zhang Y P, Gao L H. Study on different responses to cinnamic acid and sub-low temperature stress on cucumber and figleaf gourd seedlings. Journal of China Agricultural University, 2014, 19(4): 86-94. (in Chinese)

[3] Ding J, Sun Y, Xiao C L, Shi K, Zhou Y H, Yu J Q. Physiological basis of different allelopathic reactions of cucumber and figleaf gourd plants to cinnamic acid. Journal of Experimental Botany, 2007, 58: 3765-3773.

[4] Yu J Q, Matsui Y. Phytotoxic substances in the root exudates of Cucumis sativus L. Journal of Chemical Ecology, 1994, 20: 21-31.

[5] Yu J Q, Matsui Y. Effects of root exudates of cucumber (Cucumis sativus) and allelochemicals on uptake by cucumber seedlings. Journal of Chemical Ecology, 1997, 23: 817-827.

[6] Qiao Y X, Zhang Y P, Zhang H X, Tian Y Q, Gao L H. Developmental characteristics and cinnamic acid resistance of root border cells in cucumber and figleaf gourd seedlings. Journal of Intergrative Agriculture, 2013, 12(11): 2065-2073.

[7] Singh P K, Singh R, Singh S. Cinnamic acid induced changes in reactive oxygen species scavengin g enzymes and protein profile in maize (Zea mays L.) plants grown under salt stress. Physiological Moleular Biology Plants, 2013, 19(1): 53-59.

[8] Yu J Q, Shou S Y, Qian Y R, Hu W H. Autotoxic potential in cucurbit crops. Plant and Soil, 2000, 223: 147-151.

[9] 乔永旭. NaCl胁迫对黄瓜根系边缘细胞发生的影响. 植物生理学报, 2011, 47(1): 97-101.

Qiao Y X. Effects of NaCl stress on generation of root border cells incucumber (Cucumis sativus L.). Plant Physiology Journal, 2011, 47(1): 97-101. (in Chinese)

[10] Xing C H, Zhu M H, Cai M Z, Liu P, Xu G D, Wu S H. Developmental characteristics and response to iron toxicity of root border cells in rice seedlings. Journal of Zhejiang University Science B, 2008, 9: 261-264.

[11] Cai M Z, Zhang S N, Xing C H, Wang F M, Wang N, Zhu L. Developmental characteristics and aluminum resistance of root border cells in rice seedlings. Plant Science, 2011, 180: 702-708.

[12] Knox O G G, Gupta V V S R, Nehl D B, Stiller W N. Constitutive expression of Cry proteins in roots and border cells of transgenic cotton. Euphytica, 2007, 154: 83-90.

[13] Jaroszuk-Scise? J, Kurek E, Rodzik B, Winiarczyk K. Interactions between rye (Secale cereale) root border cells (RBCs) and pathogenic and nonpathogenic rhizosphere strains of Fusarium culmorum. Mycological Research, 2009, 113: 1053-1061.

[14] Zhang Y P, Jia F F, Zhang X M, Qiao Y X, Shi K, Zhou Y H, Yu J Q. Temperature effects on the reactive oxygen species formation and antioxidant defence in roots of two cucurbit species with contrasting root zone temperature optima. Acta Physiol Plant, 2012, 34: 713-720.

[15] Zhang Y P, Qiao Y X, Zhang Y L, Zhou Y H, Yu J Q. Effects of root temperature on leaf gas exchange and xylem sap abscisic acid concentrations in six cucurbitaceae species. Photosynthetica, 2008, 46(3): 356-362.

[16] 陈可, 孙吉庆, 李敏. 丛枝菌根真菌对西瓜嫁接苗生长和根系防御性酶活性的影响. 应用生态学报, 2013, 24(1): 135-141.

Chen K, Sun J Q, Liu R J, Liu M. Effects of arbuscular mycorrhizal fungus on the seedling growth of grafted watermelon and the defensive enzyme activities in the seedling roots. Chinese Journal of Applied Ecology, 2013, 24(1): 135-141. (in Chinese)

[17] Canals R M, Emeterio L S, Peralta J. Autotoxicity in Lolium rigidum: analysing the role of chemically mediated interactions in annual plant populations. Journal of Theoretical Biology, 2005, 235: 402-407.

[18] Ye S F, Zhou Y H, Sun Y, Zou L Y, Yu J Q. Cinnamic acid causes oxidative stress in cucumber roots, and promotes incidence of Fusarium wilt. Environmental and Experimental Botany, 2006, 56: 255-262.

[19] Doke N, Miura Y, Sanchez L M, Kawakita K. Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants. Foyer C H, Mullineaux P M. (CRC Press, Boca Raton, FL), 1994: 177-199.

[20] Olson P D, Varner J E. Hydrogen peroxide and lignification. Plant Journal, 1993, 4: 887-892.

[21] Parida A K, Das A B, Mohanty P. Defense potentials to NaCl in a mangrove, Bruguiera parviflora: differential changes of isoforms of some antioxidative enzymes. Journal of Plant Physiology, 2004, 161: 531-542.

[22] Asaduzzaman M, Kobayashi Y, Isogami K, Tokura M, Tokumasa K, Asao T. Growth and yield recovery in strawberry plants under autotoxicity through electrodegradation. European Journal of Horticultural Science, 2012, 77(2): 58-67.

[23] Wang L J, Huang W D, Li J Y, Liu Y F, Shi Y L. Peroxidation of membrane lipid and Ca²⁺ homeostasis in grape mesophyll cells during the process of cross-adaptation to temperature stresses. Plant Science, 2004, 167: 71-77.

[24] Ali M B, Hahn E J, Paek K Y. Effects of light intensities on antioxidant enzymes and malondialdehyde content during short-term acclimatization on micropropagated Phalaenopsis plantlet. Environmental and Experimental Botany, 2005, 54: 109-120.

[25] Martinez C A, Loureiro M E, Oliva M A, Maestri M. Differential responses of superoxide dismutase in freezing resistant Solanum curtilobum and freezing sensitive Solanum tuberosum subjected to oxidative and water stress. Plant Science, 2001, 160: 505-515.

[26] Song G L, Hou W H, Wang Q H, Wang J L, Jin X C. Effect of low temperature on eutrophicated waterbody restoration by Spirodela polyrhiza. Bioresource Technology, 2001, 97: 1865-1869.

[27] 尚庆茂, 宋士清, 张志刚. 水杨酸增强黄瓜幼苗耐盐性的生理机制. 中国农业科学, 2007, 40(1): 147-152.

Shang Q M, Song S Q, Zhang Z G. Physiological mechanisms of salicylic acid enhancing the salt tolerance of cucumber seedling. Scientia Agricultura Sinica, 2007, 40(1): 147-152. (in Chinese)

[28] 乔永旭. 低温处理过程中水杨酸对红掌叶片生理指标的影响. 东北林业大学学报, 2010, 38(2): 11-12.

Qiao Y X. Effect of salicylic acid on physiological index of Anthurium andraeanum leaves at low temperature. Journal of Northeast Forestry University, 2010, 38(2): 11-12. (in Chinese)

[29] 庞金安, 马德华, 霍振荣, 李淑菊. 水杨酸预处理对提高黄瓜幼苗耐低温能力的影响. 华北农学报, 2000, 15(1): 112-115.

Pang J A, Ma D H, Huo Z R, Li S J. Effect of salicylic acid on chilling resistance of cucumber seedlings. Acta Agriculturae Boreali-Sinica, 2000, 15(1): 112-115. (in Chinese)

[30] Hiser C, McIntosh L. Alternative oxidase of potato is an integral membrane protein synthesized de novo during aging of tuber slices. Plant Physiology, 1990, 93: 312-318.

[31] Laties G G. The cyanide-resistant, alternative path in higher plant respiration. Annual Review of Plant Physiology, 1982, 33: 519-555.

[32] 杨素苗, 李保国, 齐国辉, 郭素萍, 胡志伟. 根系分区交替灌溉对苹果根系活力、树干液流和果实的影响. 农业工程学报, 2010, 26 (8): 73-79.

Yang S M, Li B G, Qi G H, Guo S P, Hu Z W. Effects of alternate partial rootzone irrigation on roots activity, stem sap flow and fruit of apple. Transactions of the CSAE, 2010, 26(8): 73-79. (in Chinese)

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Effect of Root Border Cells on Reactive Oxygen Metabolism and Root Activity of Cucumber and Figleaf Gourd Seedlings Under Cinnamic Acid Stress

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