根系边缘细胞对肉桂酸胁迫下黄瓜和黑籽南瓜活性氧代谢与根系活力的影响

doi:10.3864/j.issn.0578-1752.2015.08.12

Abstract

Abstract: 【Objective】The aim of this paper was to clarify the defensive mechanism of root border cells (RBC) against cinnamic acid (CA) stress by analyzing the reactive oxygen metabolism and root activity of seedling RBC in cucumber (Cucumis sativus L.) and figleaf gourd(Cururbita ficifolia) under CA stress. 【Method】 The 5 mm length of aeroponicly cultured roots of cucumber cultivar Zhongnong No. 16 andfigleaf gourd cultivar bouche were divided into two groups: one group was used to investigate reactive oxygen metabolism by spraying with 0 and 0.25 mmol·L^-1 CA at 1 h interval, another group rinsing root tips of distilled water once every 4 h firstly and RBC were removed, then spraying 0 and 0.25 mmol·L^-1 CA every 1 h. The metabolic index of active oxygen was measured after treatment for 0, 12, 24 and 36 hours of CA stress, root fresh weight, root respiration rate and root activity of the seedlings were measured after 24 hours. 【Result】The root fresh weight and physiological metabolism were not affected significantly by RBC without CA stress. If the RBC were not removed, the root fresh weight and root activity decreased and the level of reactive oxygen species (ROS) and malonaldehyde (MDA), total respiration rate, CN-resistant respiration rate, the activities of superoxide dismutase (SOD), catalase (CAT) and guaiacol-dependent peroxidase (POD) increased significantly in cucumber under CA stress. However, the root fresh weight, root activity, the level of ROS and MDA, total respiration rate, CN-resistant respiration rate, the activities of SOD, CAT and POD were not affected significantly in figleaf gourd seedlings. If RBC were removed, the effect of CA stress on figleaf gourd was similar with cucumber, but significantly than RBC not removing. 【Conclusion】 RBC possibly attenuated the CA toxicity to the roots of cucumber and figleaf gourd through decreasing ROS and MDA content. The defensive ability of RBCs against CA on figleaf gourd was stronger than that of cucumber. Removal of RBCs could lead to great damage to the roots of cucumber and figleaf gourd significantly under CA stress.

Key words: cucumber, figleaf gourd, cinnamic acid, root border cells, reactive oxygen species

QIAO Yong-xu, ZHANG Yong-ping, GAO Li-hong. Effect of Root Border Cells on Reactive Oxygen Metabolism and Root Activity of Cucumber and Figleaf Gourd Seedlings Under Cinnamic Acid Stress[J].Scientia Agricultura Sinica, 2015, 48(8): 1579-1587.

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Effect of Root Border Cells on Reactive Oxygen Metabolism and Root Activity of Cucumber and Figleaf Gourd Seedlings Under Cinnamic Acid Stress

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