Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (15): 3079-3086.doi: 10.3864/j.issn.0578-1752.2013.15.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Functional Characterization of the Plasma Intrinsic Protein Gene OsPIP2;6 in Rice

 LI  Rong-1, NIU  Xiang-Li-2, MIAO  Yan-Wen-1, XIONG  Fang-Jie-1, LIU  Yong-Sheng-12   

  1. 1.School of Life Sciences, Chongqing University, Chongqing 400030
    2.School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009
  • Received:2013-03-11 Online:2013-08-01 Published:2013-05-22

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Abstract: 【Objective】 The current study aims to investigate the functional significance of a rice aquaporin (OsPIP2;6) by analyzing the phenotypic alteration resulting from overexpressing OsPIP2;6 in responses to stressed conditions.【Method】The overexpression vector of 35S::pHB-OsPIP2;6 was constructed and introduced into rice cv. Nipponbare by Agrobacterium-mediated transformation .The phenotypic analysis and molecular detection of transgenic plants were performed to verify the function of OsPIP2;6. 【Result】 Real-time PCR indicated that OsPIP2;6 was regulated by gibberellin (GA), abscisic acid (ABA) and the expression levels of OsPIP2;6 in transgenic lines were significantly increased. There was no obvious difference in growth between transgenic plants and wild type under normal condition. The performance of T2 transgenic seedlings revealed that OsPIP2;6 overexpression lines showed increased growth rates under stresses condition. 【Conclusion】 The phenotype of overexpressing OsPIP2;6 lines suggested a role of OsPIP2;6 in resistance to draught, waterlogging, and salt stress.

Key words: Oryza sativa L. , aquaporin , draught , salt stress

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