逆境诱导基因GsSNARE1的耐逆功能分析

doi:10.3864/j.issn.0578-1752.2013.11.002

Abstract

Abstract: 【Objective】The aim of this study is to isolate GsSNARE1, characterize its function under environmental stress, and provide an important basis for studying the precise function and molecular mechanism of GsSNARE1.【Method】Glycine soja 50109 was used as gene cloning material, and the interaction between GsSNARE1 and GsCBRLK was verified by yeast two hybrid analysis. Real-time PCR analysis was used to analyze the expression profile of GsSNARE1 under stress conditions and in different plant tissues. The GsSNARE1 protein was expressed in E. coli, and its function was analyzed under salt and drought stresses.【Result】In this study, the GsCBRLK interacting protein GsSNARE1 was isolated, the full length GsSNARE1 gene was cloned, and the interaction between GsSNARE1 and GsCBRLK in yeast NMY51 was verified. Real-time PCR analysis showed that expression of GsSNARE1 was greatly induced by salt and drought stresses, and PLACE analysis revealed a serial of stress-related cis-elements in GsSNARE1 promoter. GsSNARE1 expressed in different tissues of G. soja. GsSNARE1 expression decreased salt and drought resistance of the recombinant E.coli.【Conclusion】GsSNARE1 interacted with GsCBRLK in yeast. GsSNARE1 transcripts were greatly accumulated under salt and drought stresses, and were detected in different tissues. Expression of GsSNARE1 in E.coli resulted in decreased salt and drought tolerance.

Key words: Glycine soja , SNARE protein , functional characterization , salt stress , drought stress

SUN Ming-Zhe, SUN Xiao-Li, YU Qing-Yue, JI Wei, CAI Hua, ZHU Yan-Ming. Functional Analysis of a Stress-Induced SNARE Gene GsSNARE1 in Response to Salt and Drought Stresses[J].Scientia Agricultura Sinica, 2013, 46(11): 2191-2200.

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Functional Analysis of a Stress-Induced SNARE Gene GsSNARE1 in Response to Salt and Drought Stresses

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