Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (21): 4428-4436.doi: 10.3864/j.issn.0578-1752.2012.21.011

• HORTICULTURE • Previous Articles     Next Articles

Accumulation of Flavanols, Expression of Leucoanthocyanidin Reductase Induced by UV-C Irradiation During Grape Berry Development

 WEN  Peng-Fei, XING  Yan-Fu, NIU  Tie-Quan, GAO  Mei-Ying, NIU  Xing-Yan   

  1. 1.山西农业大学园艺学院，山西太谷 030801
  • Received:2012-07-26 Online:2012-11-01 Published:2012-09-13

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Abstract: 【Objective】 The effect of ultraviolet C on the accumulation of flavanols in grape berry during its development was studied.【Method】The 5-year old grapevine (Vitis vinifera L. cv. Cabernet Sauvignon) was subjected to the regular UV-C irradiation during berry development, and the accumulation of flavanols, the enzyme activity of leucoanthocyanidin reductase (LAR), the transcriptional level and translation level of Vv lar1 and lar2 were determined by spectrophotometer, real time PCR, and Western Blot.【Result】 The accumulation pattern of flavanols in grape berry during its development was not changed, whereas the accumulation of flavanols was promoted by UV-C irradiation, especially in the young berry. In addition, the activity of LAR, the key enzyme in the flavanols biosynthetic pathway, was induced by the UV-C irradiation. The synthesis of LAR1 and LAR2 new protein and the accumulation of Vv lar1 and lar2 mRNA induced by UV-C irradiation were also observed. 【Conclusion】 Results of the study indicated that the UV-C irradiation could induce the transcription of Vv lar1 and Vv lar2, the synthesis of LAR1 and LAR2 new protein, the increase of LAR activity, and resulted in the accumulation of flavanols in berry.

Key words: grape berry, UV-C, flavanols, LAR

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