Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (9): 1766-1774.doi: 10.3864/j.issn.0578-1752.2012.09.012

• HORTICULTURE • Previous Articles     Next Articles

Effects of Different Rootstocks on Ripening Quality, Activities and Gene Expression of Aroma-Related Enzymes in Grafting Oriental Melon (Cucumis melo var. makuwa Makino)

 TIAN  Xiao-Bin, QI  Hong-Yan, LI  Yan, JIN  Ya-Zhong, LIU  Wen-Wei, XU  Xiao-Fei   

  1. 1.沈阳农业大学园艺学院/设施园艺省部共建教育部重点实验室/辽宁省设施园艺重点实验室，沈阳 110866
  • Received:2011-12-30 Online:2012-05-01 Published:2012-03-15

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Abstract: 【Objective】The effects of different rootstocks grafting on main quality, kinds and content of aroma compounds, aroma-related enzymes activities and gene expression of mature oriental sweet melon fruits were studied.【Method】 The oriental sweet melon cultivar ‘Yumeiren’ was used as scion, the white seed pumpkin (Cucurbita moschata Duch.) ‘Shengzhen1’(as treatment G1), ‘Tianzhen2’ (as treatment G2), muskmelon ‘PG22HF1’(Cucumis melo) (as treatment G3) were used as rootstocks, the ripening quality and aroma-related enzymes activities were measured, then the related expression levels of related genes, including alcohol dehydrogenase (ADH) and alcohol acyltransferase (AAT) were obtained from fruits on different rootstocks. 【Result】 Three rootstocks grafting have different levels of delaying fruit ripening, as firmness increased, rind degreening slowed down and soluble solids content lowered. ‘Tianzhen2’ ripens about two days later than own-root fruits, while, it was delayed more than two days in the other treatments. Rootstocks ‘Shengzhen1’ and ‘PG22HF1’ havd higher influence on fruit maturity compared to ‘Tianzhen2’. The kinds and content of non-acetate esters were significantly increased, main esters content was increased by ‘Tianzhen2’, on the same day after anthesis the content of non-acetate esters was 1.5 times higher than own-root fruits, while it was significantly decreased in the other treatments. Allyl isohexyl oxalate was found in all three rootstocks grafting, while, undetected in own-root fruits. For the same mature fruits, aroma-related enzymes activities were not significantly decreased by grafting, while increased by ‘Tianzhen2’. However, grafting suppressed the gene expression of aroma-related enzymes.【Conclusion】The effects of different varieties of rootstocks grafting on ripening quality was different. ‘Tianzhen2’ contributes for improvement of fruit quality, while the others reduced fruit quality. On one hand, grafting delayed fruits ripening, and therefore affected fruit quality and aroma-related enzymes activities. On the other hand, it affected aroma compounds and fruit quality by suppressing the gene expression of aroma-related enzymes.

Key words: oriental melon, graft, quality, aroma-related enzymes, gene expression

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