Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (9): 1758-1765.doi: 10.3864/j.issn.0578-1752.2012.09.011

• HORTICULTURE • Previous Articles     Next Articles

Molecular Cloning and Sequence Analysis of Acyl-CoA Oxidase Gene from ‘Hujingmilu’ Peach Fruit

 QI  Yu-Jie, ZHANG  Xin, YANG  Xia, GAO  Zhong-Shan, JIA  Hui-Juan   

  1. 浙江大学果树科学研究所，杭州 310058
  • Received:2011-09-13 Online:2012-05-01 Published:2012-01-30

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Abstract: 【Objective】The aim of this study is to clone and analyse the five genes of acyl-CoA oxidase(ACX) from peach (Prunus persica L. Batsch) flesh. 【Method】Specific primers were designed based on the genome of peach of ACX published on Phytozome (www.phytozome.org), and the full-length of the five genes were cloned. 【Result】 In this study, the full-length of the five genes of ACX from peach fruit was obtained. There was no mutation in the CDS of PpACX1 and PpACX3. A mutates to G in PpACX2 on seq 871, which leads to Gln mutates to Arg. In PpACX4, there are two same sense mutations in seq 80 and 368. A mutates to c in PpACX5 on seq 475, which leads to Met mutates to Leu. The amino acid sequence of peach ACX was highly homologous with other species, especially tomato, squash, barley, and grape. Phylogenetic analysis showed that five gene family members were in the same evolutionary branch except PpACX4 which had a more closer relationship with pumpkin, grape, tomato, soybean, etc. PpACX1 was proved to be the highest expression member through the gene expression in root, stalk, leaf and fruit.【Conclusion】There are some differences between the 5 expressed genes of ACX cloned from ‘Hujingmilu’ peach fruit and the database of the genome. PpACX1 was proved to be the most important gene member in peach fruit.

Key words: Prunus persica L. Batsch, Acyl-CoA oxidase, gene cloning, sequence analysis

[1]Jia H J, Okamoto G. Distribution of volatile compounds in peach fruit. Journal of Japanese Society for Horticultural Science, 2001, 70: 223-225.

[2]Schwab W, Davidovich-Rikanati R, Lewinsohn E. Biosynthesis of plant-derived flavor compounds. The Plant Journal, 2008, 54: 712-732.

[3]Husain Q, Hui Y H. Chemistry and biochemistry of some vegetable flavors//Handbook of Fruit and Vegetable Flavours. JohnWiley & Sons//Hoboken, New Jersey, 2010: 575-625.

[4]Arent S, Pye V E, Henriksen A. Structure and function of plant acyl-CoA oxidases. Plant Physiology and Biochemistry, 2008, 46: 292-301.

[5]Lazarow P B, Duve C A. Fatty acyl-coa oxidizing system in rat liver peroxisomes enhancement by clofibrate a hypolipidemic drug. Proceedings of the National Academy of Sciences of the USA, 1976, 73: 2043-2046.

[6]Kawamoto S, Nozaki C, Tanaka A, Fukui S. Fatty acid β-oxidation system in microbodies of n-alkane-grown Candida tropicalis. European Journal of Biochemistry, 1978, 83: 609-613.

[7]Kawaguchi A, Tsubotani S, Seyama Y, Yamakawa T, Osumi T, Hashimoto T, Kikuchi T, Ando M, Okuda S. Stereochemistry of dehydrogenation catalyzed by Acyl-CoA oxidase. The Journal of Biochemistry, 1980, 88: 1481-1486.

[8]Coopers T G, Harry B. β-Oxidation in glyoxysomes from castor bean endosperm. The Journal of Biological Chemistry, 1969, 244(13): 3514-3520.

[9]Wang H J, Dall M T, Waché Y. Evaluation of acyl coenzyme A oxidase (ACOX) isozyme function in the n-alkane-assimilating yeast Yarrowia lipolytica. Journal of Bacteriology, 1999, 181: 5140-5148.

[10]Waché Y, Pagot Y, Nicaud J M, Belin J M. Acyl-CoA oxidase, a key step for lactone production by Yarrowia lipolytica. Journal of Molecular Catalysis B: Enzymatic, 1998, 165-169.

[11]Waché Y, Aguedo M, Choquet A, Gatfield I L, Nicaud J M, Belin J  M. Role of beta-oxidation enzymes in gamma-decalactone production by the yeast Yarrowia lipolytica. Applied and Environmental Microbiology, 2001, 67: 5700-5704.

[12]Waché Y, Husson F, Feron G. Yeast as an efficient biocatalyst for the production of lipid-derived flavours. Antonie van Leeuwenhoek, 2006, 89: 405-416.

[13]Hooks M A, Bode K, Couee I. Regulation of Acyl-CoA oxidases in maize seedlings. Phychemistry, 1995, 40(3): 657-660.

[14]Hayashi H, Bellis D L, Yamaguchi K, Kato A. Molecular characterization of a glyoxysomal long chain acyl-CoA oxidase that is synthesized as a precursor of higher molecular mass in pumpkin. Journal of Biochemistry, 1998, 273: 8301-8307.

[15]Rachel A, Christopher L, Anthony L. Structure determination and analysis of acyl-CoA oxidase (ACOX1) from tomato. Acta Crystallographica, 2006, D62: 683-686.

[16]Ameeta K A, Youlin Q, Deepti G B, Woerner B M, Brown S M. Gene isolation and characterization of two acyl-CoA oxidases from soybean with broad substrate specificities and enhanced expression in the growing seedling axis. Plant Molecular Biology, 2001, 47: 519-531.

[17]Xi W P, Zhang B, Liang L, Shen J Y, Wei W W, Xu C J, Allan A C, Ferguson I B, Chen K S. Postharvest temperature influences volatile lactone production via regulation of acyl-CoA oxidases in peach fruit. Plant, Cell and Environment, 2012, 35(3): 534-545.

[18]陈长宝, 朱树华, 周  杰. 改良CTAB法提取成熟肥城桃果实的总RNA. 山东农业科学, 2009(5): 102-104.

Chen C B, Zhu S H, Zhou J. Improved CTAB method for total RNA extraction of mature Feicheng peach fruit. Shandong Agricultural Science, 2009(5): 102-104. (in Chinese)

[19]Sudhir K, Koichiro T, Matoshi N. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics, 2004, 5(2): 150-163.

[20]Tong Z, Gao Z, Wang F, Zhou J, Zhang Z. Selection of reliable reference genes for gene expression studies in peach using real-time PCR. Molecular Biology, 2009, 10: 71.

[21]Jose M O, Ana G P, Jose J R, Luis C S. Aroma of virgin olive oil: Biogenesis of the “Green” odor notes. Journal of Agricultural and Food Chemistry, 1993, 41(12): 2368-2373.

[22]Helena P, Mario R S. Plant lipoxygenases. physiological and molecular features. Plant Physiology, 2002, 130(9): 15-21.

[23]Akikazu H. The biogeneration of green odour by green leaves. Phytochemistry, 1993, 34(5): 1201-1218.

[24]Baker A, Graham I A, Holdsworth M, Smith S M, Theodoulou F L. Chewing the fat: oxidation in signaling and development. Trends in Plant Science, 2006, 11: 124-132.

[25]Kirsch T, Loffler H G, Kindl H. Plant acyl-CoA oxidase: Purification, characterization and monomeric apoprotein. Journal of Biochemistry, 1986, 18: 8570-8575.

[26]Hooks M A, Bode K, Couee I. Higher-plant medium- and short-chain acyl-CoA oxidases: Identification, purification and characterization of two novel enzymes of eukaryotic peroxisomal beta-oxidation. Biochemical Journal, 1996, 320(2): 607-614.

[27]刘巍立. 桃果实成熟软化过程中特征香气物质形成相关酶活性的变化研究[D]. 杭州: 浙江大学, 2010.

Liu W L. Study on the characteristics aroma and related enzyme activity during ripening of peach (Prunus persica L.) fruit[D]. Hangzhou: Zhejiang University, 2010.(in Chinese)
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