Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (9): 1676-1684.doi: 10.3864/j.issn.0578-1752.2012.09.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification and Genetic Analysis of a Cytoplasmic Male Sterile Line in Maize (Zea mays L.)

 ZHU  Li-Ying, CHEN  Jing-Tang, HUANG  Ya-Qun, ZHAO  Yong-Feng, SONG  Zhan-Quan   

  1. 1.河北农业大学农学院/国家玉米改良中心河北分中心/“华北作物种质资源研究与利用”省部共建教育部重点实验室，河北保定 071001
    2.保定学院，河北保定 071051
  • Received:2011-11-02 Online:2012-05-01 Published:2011-12-21

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Abstract: 【Objective】 A new cytoplasmic male sterile line (Nongxi 928cms-Q1261) was identified for type of male sterile, stage of microspore abortion and genetic mechanism for its application to maize breeding. 【Method】 The male sterile expression was observed by planting sterile line in different locations and years. Testcross, sister cross and reciprocal cross were conducted with Nongxi 928cms-Q1261 to analyze the genetic characteristics of its male sterility. Meanwhile, the cytoplasmic type was discriminated by PCR amplification with specifically primers and the microspore genesis was observed under optical microscope by paraffin section technique.【Result】Results showed that the male sterility characteristics of Nongxi 928cms-Q1261 were stable, no anthers exposed on tassel and pollen aborted completely. The cytoplasmic male sterile gene, which was classified into S type of CMS, originated from Q1261, and its nuclear sterile gene originated from Nongxi 928, which was controlled by a pair of recessive genes. The microspores started degrading at the late stage of microspore and degenerated completely at the mature pollen stage. The male sterility of Nongxi 928cms-Q1261 could be maintained by inbred line Zheng58.【Conclusion】Nongxi 928cms-Q1261 belongs to S-type of CMS and is a non-pollen-type cytoplasmic male sterile line with many restorer lines.

Key words: maize, cytoplasmic male sterile, fertility identification, genetic analysis, cytoplasm classification

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