Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (7): 1355-1364.doi: 10.3864/j.issn.0578-1752.2012.07.013

• HORTICULTURE • Previous Articles     Next Articles

The Association Analysis of Phenotypic Traits with SRAP Markers in Chrysanthemum

 LI  Ren-Wei, WANG  Chen, DAI  Si-Lan, LUO  Xin-Yan, LI  Bao-Qin, ZHU  Jun, LU  Jie, LIU  Qian-Qian   

  1. 北京林业大学园林学院/国家花卉工程技术研究中心，北京 100083
  • Received:2011-09-01 Online:2012-04-01 Published:2012-03-28

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Abstract: 【Objective】 In order to provide a genetic basis for studies on complex quantitive traits and for molecular assisted breeding of chrysanthemum, the SRAP markers associated with important chrysanthemum horticulture traits were screened.【Method】 The genomic regions with selection sweep were detected through scanning 58 representative chrysanthemum cultivars using 19 SRAP markers. Population structure was firstly analyzed, then association analysis between SRAP markers and 18 important phenotypic traits were performed using TASSEL GLM.【Result】 Genetic structure analysis showed that the selected cultivar population was composed of 5 subpopulations, namely flat type subgroup, tube type subgroup, irregular type subgroup, anemone type subgroup and Japanese subgroup. There were 6 SRAP loci associated with 5 quantitative characters (P＜0.01), among which 3 flower traits were associated with 5 loci, while 1 stem and 1 leaf traits were associated with 1 locus, respectively. The rate of explanation on the phenotype of related locus ranged from 0.0738 to 0.4791.【Conclusion】It is feasible to estimate and differentiate chrysanthemum population’s structure effectively using SRAP markers, and the markers obtained in this study are promising in molecular assisted breeding.

Key words: Chrysanthemum×morifolium, phenotypic traits, SRAP, association analysis

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