大豆开花盛期快速叶绿素荧光参数的QTL分析

doi:10.3864/j.issn.0578-1752.2011.24.002

Abstract

Abstract: 【Objective】 The present study is aimed to identify QTL associated with fast chlorophyll fluorescence parameters (JIP parameters), examine the genetic relationships among different parameters, and compare the genetic base underlying the parameters between the two growth stages of R2 and R6 in soybean. 【Method】 A pot experiment was carried out to evaluate five JIP parameters at R2 growth stage using 184 recombinant inbred lines (RILs) derived from a cross between two varieties of Kefeng 1 and Nannong 1138-2, and then the QTL of above parameters were detected and mapped. 【Result】 A total of 16 QTL, located on linkage groups (LGs) A1, C2, D2, I, M, N and O, respectively, were identified, and explained phenotypic variation ranging from 4.40% to 20.06% with the LOD score from 2.40 to 5.65. Three major genomic regions were detected to be associated with several parameters simultaneously, which were between markers Satt286 and Satt316 on LG C2, marker Sat_418 and Satt650 on LG I, and marker Sat_231 and sat_196 on LG O, respectively. 【Conclusion】 Different JIP parameters might be controlled by the same or different genes. Most of the QTL associated with JIP parameters were not detected consistently at R2 and R6 growth stage, indicating that the genetic mechanism underlying the JIP parameters is relatively complicated. The marker interval between Sat_231 and Sat_196 on LG O was detected at both R2 and R6 growth stage, and this genomic region may contain stably expressing genes underlying the intrinsic features of the photosynthetic apparatus and be useful in the breeding practice of soybean.

Key words: soybean, fast chlorophyll fluorescence parameters (JIP parameters), recombinant inbred line (RIL), QTL mapping

YIN Zhi-Tong, MENG Fan-Fan, SONG Hai-Na, CHAO Mao-Ni, XU Xiao-Ming, DENG De-Xiang, YU De-Yue. QTL Mapping for Fast Chlorophyll Fluorescence Parameters in Soybean[J].Scientia Agricultura Sinica, 2011, 44(24): 4980-4987.

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QTL Mapping for Fast Chlorophyll Fluorescence Parameters in Soybean

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