Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (24): 4980-4987.doi: 10.3864/j.issn.0578-1752.2011.24.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

QTL Mapping for Fast Chlorophyll Fluorescence Parameters in Soybean

 YIN  Zhi-Tong, MENG  Fan-Fan, SONG  Hai-Na, CHAO  Mao-Ni, XU  Xiao-Ming, DENG  De-Xiang, YU  De-Yue   

  1. 1.南京农业大学国家大豆改良中心/作物遗传与种质创新国家重点实验室，南京 210095
    2.扬州大学农学院/作物遗传生理江苏省重点实验室/植物功能基因组学教育部重点实验室，江苏扬州 225009
    3.南京农业大学生命科学学院，南京210095
  • Received:2011-06-22 Online:2011-12-15 Published:2011-10-17

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Abstract: 【Objective】 The present study is aimed to identify QTL associated with fast chlorophyll fluorescence parameters (JIP parameters), examine the genetic relationships among different parameters, and compare the genetic base underlying the parameters between the two growth stages of R2 and R6 in soybean. 【Method】 A pot experiment was carried out to evaluate five JIP parameters at R2 growth stage using 184 recombinant inbred lines (RILs) derived from a cross between two varieties of Kefeng 1 and Nannong 1138-2, and then the QTL of above parameters were detected and mapped. 【Result】 A total of 16 QTL, located on linkage groups (LGs) A1, C2, D2, I, M, N and O, respectively, were identified, and explained phenotypic variation ranging from 4.40% to 20.06% with the LOD score from 2.40 to 5.65. Three major genomic regions were detected to be associated with several parameters simultaneously, which were between markers Satt286 and Satt316 on LG C2, marker Sat_418 and Satt650 on LG I, and marker Sat_231 and sat_196 on LG O, respectively. 【Conclusion】 Different JIP parameters might be controlled by the same or different genes. Most of the QTL associated with JIP parameters were not detected consistently at R2 and R6 growth stage, indicating that the genetic mechanism underlying the JIP parameters is relatively complicated. The marker interval between Sat_231 and Sat_196 on LG O was detected at both R2 and R6 growth stage, and this genomic region may contain stably expressing genes underlying the intrinsic features of the photosynthetic apparatus and be useful in the breeding practice of soybean.

Key words: soybean, fast chlorophyll fluorescence parameters (JIP parameters), recombinant inbred line (RIL), QTL mapping

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