中国主栽银耳配对香灰菌的系统发育和遗传多样性

doi:10.3864/j.issn.0578-1752.2010.03.015

Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (3): 552-558 .doi: 10.3864/j.issn.0578-1752.2010.03.015

• HORTICULTURE • Previous Articles Next Articles

Phylogeny and Genetic Diversity of Annulohypoxylon spp. Paired with Cultivated Tremella fuciformis Berk.

WEN Wen-ting, JIA Ding-hong, GUO Yong, SUN Qun, YANG Zhi-rong, PENG Wei-hong

（四川大学生命科学学院）

Received:2009-05-05 Revised:2009-07-10 Online:2010-02-10 Published:2010-02-10
Contact: PENG Wei-hong

Abstract

Abstract:

【Objective】 The aim of this paper is to study the classification and genetic diversity of Xianghui and to provide biological information on utilization and breeding of Xianghui. 【Method】 The phylogeny of 16 strains of Xianghui, which were collected from different provinces and paired with cultivated Tremella, was determined by ITS sequences analysis. Furthermore, the genetic diversity of these strains was analyzed by inter-simple sequence repeat (ISSR). 【Result】 The ITS sequences of the 16 strains were analyzed and the phylogenic tree was constructed, and the results revealed that the homology between Xianghui and Annulohypoxylon stygium was 99%. Among the 70 ISSR primers, 11 with high polymorphism were selected and a total of 62 ISSR markers were obtained. At 0.7 similarity distance, cluster analysis revealed the existence of five distinct genetic groups. 【Conclusion】 The 16 strains of Annulohypoxylon spp. investigated showed low genetic similarity and genetic variations at DNA level significantly.

Key words: Tremella fuciformis, accompanying fungi, genetic diversity, cluster analysis

WEN Wen-ting,JIA Ding-hong,GUO Yong,SUN Qun,YANG Zhi-rong,PENG Wei-hong
. Phylogeny and Genetic Diversity of Annulohypoxylon spp. Paired with Cultivated Tremella fuciformis Berk.
[J].Scientia Agricultura Sinica, 2010, 43(3): 552-558 .

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