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    10 February 2010, Volume 43 Issue 3
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    QTLs Mapping for Cold Tolerance at Seedling Stage in Dongxiang Wild Rice (Oryza rufipogon Griff.)
    XIA Rui-xiang,XIAO Ning,HONG Yi-huan,ZHANG Chao,SU Yan,ZHANG Xiao-meng,CHEN Jian-min
    Scientia Agricultura Sinica. 2010, 43(3):  443-451 .  doi:10.3864/j.issn.0578-1752.2010.03.001
    Abstract ( 1044 )   PDF (719KB) ( 825 )   Save
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    【Objective】 The objective of the study is to identify quantitative trait loci (QTLs)controlling cold tolerance at seedling stage and linkage SSR markers in Dongxiang wild rice. 【Method】 Cross was made between cold tolerant Dongxiang wild rice (Oryza rufipogon Griff.) and cold susceptible rice variety Nanjing11. Nanjing 11 was then used as the recurrent parent to construct a backcross segregation population (BC2F1) which includes 144 plants. This population was used to detect QTLs for cold tolerance at seedling stage. Root conductivity was used as the index of cold tolerance. The molecular map was constructed with SSR markers and the QTLs for cold tolerance were identified by multiple trait composite intervals mapping. 【Result】 The two QTLs for cold tolerance (qRC-10-1 and qRC-10-2) were detected on chromosome 10, explaining 34.13% and 37.02% of phenotypic variances, respectively. More SSR markers were screened around the region RM171 marker where these two QTLs were located. Three QTLs in regions qRC-10-1 and qRC-10-2 were detected on chromosome 10. 【Conclusion】 qRC-10-1 and qRC-10-2 may be the major QTLs for cold tolerance at seedling stage in Dongxiang wild rice. The location of two QTLs was at the interval RM304 and RM1108 on chromosome 10. The markers in the region between RM304 and RM1108 may be used to select for cold tolerance in rice.

    Development and Mapping of EST-SSR Markers in Wheat
    PAN Hai-tao,WANG Jun-jun,WANG Ying-ying,QI Zhao-liang,LI Si-shen
    Scientia Agricultura Sinica. 2010, 43(3):  452-461 .  doi:10.3864/j.issn.0578-1752.2010.03.002
    Abstract ( 1018 )   PDF (730KB) ( 1805 )   Save
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    【Objective】 The objective of this study was to develop new simple sequence repeat (SSR) markers from EST (Expressed sequence tag) sequences (EST-SSR markers) for wheat. 【Method】 The wheat EST sequences from GenBank/dbEST (from April 18, 2006 to February 4, 2007) were used to search for SSRs, EST-SSR primer pairs were designed using Primer5.0 software, the validity of primer pairs were detected using three wheat varieties, and the marker loci were mapped using RIL population and Mapmaker/Exp3.0 software.【Result】From the 265 362 wheat EST sequences released in GenBank/dbEST, 6 314 SSRs were found which amounts to 2.38% of the total number of ESTs. Among those EST-SSRs, the most motifs were dinucleotides and trinucleotides, which were 2 237 (35.43%) and 2 084 (33.01%), respectively. For dinucleotides, GA/CT (17.85%) and AG/TC (10.37%) were the most frequent repeat, followed by CA/GT (4.07%) and AC/TG (2.53%); and for trinucleotides, CAA/GTT (3.93%), CGG/GCC (3.83%), CGC/GCG (3.36%), GGC/CCG (3.14%), CTT/GAA (2.53%) and TGC/ACG (2.27%) appeared high frequency. Based on these EST-SSR sequences, 596 EST-SSR primer pairs were designed, 194 of which were synthesized, and 165(85%)led to PCR amplification products. Using a RIL population, the polymorphism of 21 EST-SSR primer pairs with 26 loci were detected, and 23 of those loci were integrated on the wheat genetic map. 【Conclusion】 A total of 165 new wheat EST-SSR markers have been developed. EST sequences are important sources for developing SSR markers.

    Construction of a Normalized Full-Length cDNA Library of Soybean Seed at Different Developmental Stages
    LI Chen,YAN Xiao-hong,ZHOU Xin-an,SHA Ai-hua,SHAN Zhi-hui,ZHOU Rong,WEI Wen-hui
    Scientia Agricultura Sinica. 2010, 43(3):  462-467 .  doi:10.3864/j.issn.0578-1752.2010.03.003
    Abstract ( 1016 )   PDF (470KB) ( 1320 )   Save
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    【Objective】 The objective of the study is to obtain full-length genes related to seed development and to provide resources for soybean genomics. 【Method】 A normalized cDNA library enriched in full-length sequences was constructed using DSN (duplex-specific nulease)-normalization method combined with SMARTTM(switching mechanism at 5’ end of RNA transcript) technique. 【Result】The titer of unamplified cDNA library was about 6.0×105 cfu/mL. The average cDNA inserts was more than 1.0 kb with a recombination rate of nearly 100%. After large-scale plasmid extraction and sequencing, 36 656 EST sequences were generated from the soybean seed cDNA library and the average length was more than 600 bp. All the EST sequences were assembled and 27 982 unigenes were gotten, which indicated that the cDNA library was a non-redundant library. 【Conclusion】 These results indicate that the normalized full-length cDNA library has been established successfully, which is convenient for further studying the molecular mechanism and gene cloning of seed development.

    Segregation Based on the Traits and Molecular Marker of Near-Isogenic Line of Sorghum bicolor×Sorghum sudanenes
    LU Xiao-ping,YUN Jin-feng,MI Fu-gui,CHEN Qiang,ZHANG Ya-hui,XUE Chun-lei
    Scientia Agricultura Sinica. 2010, 43(3):  468-473 .  doi:10.3864/j.issn.0578-1752.2010.03.004
    Abstract ( 940 )   PDF (415KB) ( 755 )   Save
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    【Objective】 Based on the traits and the molecular markers of Sorghum (Sorghum bicolor)× Sudan grass (Sorghum sudanenes), the near-isogenic lines (NILs) of plant height, leaf number and related molecular markers were determined from the recombinant inbred line (RIL) F6:7 family. The material could be used for the QTLs analysis. 【Method】 The recombinant inbred line F6:7 family came from F2:3 genetic mapping population of Sorghum314A×Brown Sudan grass,to the traits of plant height and leaf number, the NILs were isolated separately based on the traits and the molecular markers. The plants with one trait difference (P≤0.05) and with heterozygous lines were selected firstly, assisted with SSR marker, deleted the heterozygous plant, two kinds of homozygote became the NILs of the traits. 【Result】 The NILs of plant height and leaf number were isolated from RIL-105 and RIL-85 separately. The NIL with umc1714-umc2247 interaction loci came from RIL-127. 【Conclusion】 The NILs would be useful to the research of the trait detailed location and isolation and interaction (epistasis) of QTLs.

    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY
    Analysis of Status and Constraints of Rice Production in the World
    ZHU De-feng,CHENG Shi-hua,ZHANG Yu-ping,LIN Xian-qing,CHEN Hui-zhe
    Scientia Agricultura Sinica. 2010, 43(3):  474-479 .  doi:10.3864/j.issn.0578-1752.2010.03.005
    Abstract ( 1053 )   PDF (250KB) ( 1676 )   Save
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    【Objective】 An approach to increase rice yield and production was discussed through analysis of the status of rice production and the main constraints limiting rice production in the world. 【Method】 Changes of rice planting area and yield and its contribution to production, and the main factors limiting rice production were analyzed. 【Result】 In the period from 1961 to 2006, rice planting area increased by 32% mainly due to the increase of the double cropping system, yield increased by 1.21 times mainly due to the application of semi-dwarf variety and hybrid, and its accompanying cultivation technology, improvement of irrigation system and chemical fertilizer application that resulted in the increase of rice production by 1.92 times. Planting area increase contributed to 27% and yield increase to 73% of the increase of rice production. Since 1960s of the 20th century, the annual growth rate of rice planting area decreased significantly. The annual growth rate of yield is becoming small. It is 2.58%,0.98% and1.16% respectively in 1980s, 1990s and after 2000. The decrease of annual growth rate of rice area and yield resulted in the decrease of annual growth rate of rice production. The annual growth rate of rice production is 2.73%, 1.48% and 0.88%, respectively, in 1980s, 1990s and after 2000. The factors limiting rice production mainly are low contribution of rice production technology to yield increase, more frequency of pest damage and natural disaster, yield declining in multiple-cropping system and low production profit. 【Conclusion】 To increase the global rice yield, there is a geat need for breeding of high-yielding variety and hybrid, practice of new Africa rice varieties in Africa, development of integrated rice management technology to reduce the yield gap and mechanized rice production technology, and enhancement of policy support for rice production.

    Comparison of Non-Hydraulic Root-Sourced Signaling and Grain Yield Satiability Under Drought Stress in Two Soybean [Glycine max (L.)Merr. ] Cultivars
    YANG Shen-jiao,XU Bing-cheng,FANG Yan,LI Feng-min
    Scientia Agricultura Sinica. 2010, 43(3):  480-488 .  doi:10.3864/j.issn.0578-1752.2010.03.006
    Abstract ( 937 )   PDF (470KB) ( 913 )   Save
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    【Objective】 To clarify the correlations of drought-induced non-hydraulic root signals (nHRS) with plant growth and grain yield formation could provide a mechanism basis for water-saving agriculture. 【Method】 Pot experiments were conducted with two soybean cultivars, i.e, Jinda 74 and Jin 24. nHRS were traced during manipulative progressive soil drying period at branching, early flowering and grain-filling stages. The effect of water deficit on plant biomass and grain yields of the cultivars were investigated under good soil conditions, high water (H) treatment in 2007 and well-watered (WW) in 2008 experiment, and drought conditions, low water (L) treatment in 2007 as well as light drought (LD) and severe drought (SD) in 2008. 【Result】 In response to soil drying, nHRS appeared earlier in Jinda 74 at branching stage, and later at early flowering and grain-filling stages than in Jin 24, while it disappeared earlier for Jinda 74 than Jin 24 at all the three growth stages. Jin 24 exhibited a wider average soil water content threshold range of nHRS (TR). Drought stress (both LD and SD treatments) significantly decreased the above-ground dry mass, root dry mass and grain yield (P<0.05), more dramatically for Jinda 74 than Jin 24 which showed a higher maintenance rate of grain yield than the former. The root/shoot ratio was significantly promoted (P<0.05) under severe drought conditions, i.e., L and SD treatment, more extensively for Jin 24 than Jinda 74. Water use efficiency (WUE) was notably higher (P<0.05) in Jin 24 than Jinda 74 under drought conditions in both experiments in 2007 and 2008. 【Conclusion】 Soybean cultivar of wider soil water content threshold range for nHRS yielded more, and exhibited lower root/shoot ratio but higher WUE and maintenance rate of grain yield under mild water deficit in pot-cultured condition.

    PLANT PROTECTION
    Analysis of T-DNA Insertional Flanking Sequence and Mutant Phenotypic Characteristics in Verticillium Dahliae
    XU Rong-qi,WANG Jia-ni,CHEN Jie-yin,DAI Xiao-feng
    Scientia Agricultura Sinica. 2010, 43(3):  489-496 .  doi:10.3864/j.issn.0578-1752.2010.03.007
    Abstract ( 1093 )   PDF (343KB) ( 1178 )   Save
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    【Objective】 The objective of the experiment is to identify the phenotype characteristics of T-DNA insertional mutants and constructing a platform of genetic transformation and functional genomics research in Verticillium dahliae. 【Method】 There were 2628 T-DNA insertional mutants of Verticillium dahliae strain V991 and flanking sequences of 15 mutants were obtained, and the insertional sites of some mutants were analyzed by using Agrobacterium tumefaciens mediated transformation and high-efficiency TAIL-PCR, respectively.【Result】 The mutant colony could be divided into three forms, which were hyphal type, sclerotium type and intermediate type. The sclerotium type colonies accounted for 7.3%. Culturing in flask, the sporulation peak of mutants was in the first 5-6 days after inoculation, and the sporulation ability of sclerotium mutants were generally higher than showing that of hyphal type mutants; VD991 could produce extracellular protease, amylase, cellulase and pectinase. Three mutants showing deleted protease secretion ability deleted, one mutant showing reduced amylase secretion ability and 6 mutants showing declined pectinase production were obtained; The virulence of the sclerotium type mutants was generally higher than that of the wild-type. Six mutants with reduced virulence were found from the virulence test of 26 mutants; The BLAST results showed that the flanking sequences of VD991 insertional mutants were 95%-100% identity to homologous sequences of Verticillium dahliae VDLs.17, and 87%-94% identity to Verticillium albo-atrum VaMs.102. 【Conclusion】 Agrobacterium tumefaciens mediated T-DNA random insertion could be used to Verticillium dahliae mutagenesis, and the production of microsclerotia is related to virulence and sporulation. The American strain VDLs.17 genome could be used as a reference sequence on genetic functional research of VD991.

    Geostatistical Analysis on Spatial Distribution of White-Backed Planthopper Nymphs
    YAN Xiang-hui,ZHAO Zhi-mo,LIU Huai,XIAO Xiao-hua,XIE Xue-mei,CHENG Deng-fa
    Scientia Agricultura Sinica. 2010, 43(3):  497-506 .  doi:10.3864/j.issn.0578-1752.2010.03.008
    Abstract ( 921 )   PDF (794KB) ( 726 )   Save
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    【Objective】 Dynamic process and spatial pattern of the white-backed planthopper (WBPH), Sogatella furcifera (Horváth) nymphs after they immigrated into the rice were studied to provide a theoretical basis for its integrated control. 【Method】Models of space variation were constructed at the directions of east-west and south-north based on the geostatistical method by using 10 survey data from the transplanting to ripening of the rice in Xiushan, Chongqing in 2008. Isoclines maps of the WBPH nymphs at each stage were created by the geostatistical software Surfer8.0 with Kriging interpolation. 【Result】 The variograms showed the higher the WBPH nymphs density was, the larger the space variation scope became. Average space variation was 38.7% caused by the random factor and 61.3% caused by the autocorrelation. The random degree of space variation became greater as the rice grew up. Space-related area was 18.99 m in the direction of east-west and 25.09 m in south-north averagely. Kriging interpolation indicated that aggregated scope in south-north was larger than in east-west. 【Conclusion】 The population of white-backed planthopper nymphs showed a clumped distribution mainly after immigrating into rice fields and south- north was the main direction for WBPH nymphs to aggregate and diffuse.

    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    Effects of Different Surface Mulching on Soil Organic Nitrogen Accumulation and Mineralization in Dryland of Northwestern China
    XIE Jia-yang,WANG Zhao-hui,LI Sheng-xiu,TIAN Xiao-hong
    Scientia Agricultura Sinica. 2010, 43(3):  507-513 .  doi:10.3864/j.issn.0578-1752.2010.03.009
    Abstract ( 859 )   PDF (254KB) ( 936 )   Save
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    【Objective】 Understanding soil nitrogen transformation and supplying process is the key for optimizing crop cultivation and nitrogen managements. 【Method】 In this paper, soils were sampled from a 5-year-long field experiment to study the effects of different soil surface mulching on soil organic nitrogen accumulation and mineralization in dryland of northwestern China. 【Result】 The results showed that compared to conventional cultivation, long term surface mulching with straw increased soil nitrogen mineralization potential but decreased mineralization rate. Mulching with plastic-film decreased nitrogen mineralization potential, but increased mineralization rate. The nitrogen mineralization potential for the conventional cultivation, straw mulching and plastic film mulching was 25.0-29.7, 23.2-25.9 and 23.3-26.2 mg?kg-1, respectively. Without N application, straw and plastic-film mulching increased soil organic nitrogen and light fraction organic nitrogen contents. When N was fertilized, straw mulching could increase soil organic nitrogen and light fraction organic nitrogen contents, while plastic-film mulching decreased their contents. At N rate of 240 kg?hm-2, the organic nitrogen content was 1.03, 0.95 and 0.96 g?kg-1, and the light fraction organic nitrogen content was 51, 35 and 37 mg?kg-1 for the conventional cultivation, straw mulching and plastic film mulching, respectively. 【Conclusion】 It is concluded that surface mulching with straw causes soil to immobilize more mineral N into mineralisable organic N, while with plastic film is not beneficial to organic nitrogen accumulation. Therefore, in order to increase crop yield, more nitrogen fertilizer is needed or supplemental N fertilizer should be applied at the key nitrogen requirement stage under straw mulching cultivation as it could increase soil N supply capacity. For plastic film mulching, more attention should be paid to application of organic fertilizer.

    Nutrient Budget and Soil Nutrient Status in Greenhouse System
    YU Hai-ying,LI Ting-xuan,ZHANG Xi-zhou
    Scientia Agricultura Sinica. 2010, 43(3):  514-522 .  doi:10.3864/j.issn.0578-1752.2010.03.010
    Abstract ( 924 )   PDF (461KB) ( 1220 )   Save
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    【Objective】 This paper researched on nutrient budget and its effects on soil nutrient status in typical greenhouse system in China to provide a basis for raising the utilization rate of fertilizers and maintaining the sustainable development of agriculture in greenhouse. 【Method】 By investigating the management of 18 representative greenhouses in Shouguang, Shandong province and analyzing both the greenhouse and open field soil samples, the soil nutrient budget and the trend of nutrient accumulation and translocation in soils were studied. 【Result】 Under greenhouse cultivation,the average annual input amounts of N, P2O5 and K2O were, respectively, 4 088 kg?hm-2, 3 655 kg?hm-2, and 3 437 kg?hm-2 and 63%, 61%, and 66% of the total input amount of N, P2O5 and K2O were provided by chemical fertilizers which were the main source of soil nutrient. In greenhouse system, the utilization rate of N,P2O5, and K2O were only 24%, 8%, and 46% and the input ratio of N,P2O5, and K2O (1﹕0.9﹕0.8) was quite different from the uptake ratio (1﹕0.3﹕1.4) , thus it caused an overplus of N,P2O5, and K2O in soil and the theoretical surplus were 3 214 kg?hm-2, 3 401 kg?hm-2, and 2 322 kg?hm-2, respectively. In the greenhouse soil, the content of organic matter, total nitrogen, nitrate nitrogen, available phosphorus and available potassium were all increased and their maximum values were observed in the topsoil (0-20 cm) , with an average value being 1.4 times, 1.9 times, 21.2 times, 5.4 times, and 3.7 times than that of the open field soil, respectively. Greenhouse soils displayed apparent phenomena of nutrients leaching downwards to the bottom soil, especially NO3-, which would cause a potential risk to the quality of groundwater in the area. 【Conclusion】 Under greenhouse cultivation, it is necessary to apply more organic manure and provide nutrients according to the crop requirements and soil nutrient supplying ability as it could not only produce high crop yield,but also beneficial to balance the soil nutrient and improve the utilization rate of fertilizers, then there will be no significant surplus of nutrients which may emit or leach out of soil into environment.

    Method for Simplification of Characteristic Carbon Sources for Biolog Analysis of Soil Microbial Community and Its Application
    LI Sheng-hua,LIU Ke-xing,LIAO Zong-wen
    Scientia Agricultura Sinica. 2010, 43(3):  523-528 .  doi:10.3864/j.issn.0578-1752.2010.03.011
    Abstract ( 840 )   PDF (320KB) ( 681 )   Save
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    【Objective】 In order to simplify the Biolog analysis, 9 kinds of characteristic carbon sources selected from the carbon resources in Biolog ECO microplate containing 10 carbon resources and added in Biolog MT microplate to analyse soil microbial cummunity where bacterial wilt of tomato occurred. 【Method】 The optimal concentration of characteristic carbon resources added in Biolog MT microplates was studied with the soil of potted tomato and three tests of verifying the carbon resource concentrations were carried out by using the soils of potted pepper without soil-borne diseases occurrence treated with different fertilization rates and the soils of field tomato and potted pepper with varied incidences of bacterial wilt. 【Result】 The result of carbon resource concentration experiment showed that the OD values of different concentrations of characteristic carbon resources were obviously different. Compared with the OD in ECO microplate, the optimal concentrations in MT microplate were determined. The results of verifying tests indicated that the soil microbial community functional diversity (Shannon index) with different incidences of crop were differentiated by characteristic carbon resources. 【Conclusion】 Therefore, the characteristic carbon resources could be used to differentiate the health soil and disease soil, and the soils with varied incidences of soil-borne diseases. The Biolog analysis can be simplified by using 9 kinds of characteristic carbon resources.

    HORTICULTURE
    Effects of Light Quality on Leaf Senescence and Activities of Antioxidant Enzymes in Cucumber Plants
    WANG Hong,JIANG Yu-ping,SHI Kai,ZHOU Yan-hong,YU Jing-quan
    Scientia Agricultura Sinica. 2010, 43(3):  529-534 .  doi:10.3864/j.issn.0578-1752.2010.03.012
    Abstract ( 935 )   PDF (354KB) ( 804 )   Save
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    【Objective】 To investigate the mechanism involved in the different responses of leaf senescence to light qualities, cucumber (Cucumis sativus L.) seedlings were exposed to white (W), purple (P), blue (B), green (G), yellow (Y), and red (R) lights for 15 d. 【Method】 The contents of chlorophyll, soluble proteins and MDA together with the expression and activities of antioxidant enzymes were measured 【Result】 P and B increased the transcript levels and activities of antioxidant enzymes, the contents of chlorophyll, soluble proteins and MDA. In contrast, G, Y and R decreased the activities of antioxidant enzymes, the contents of chlorophyll, soluble proteins and MDA. 【Conclusion】 These results indicate that P and B prevented senescence by maintaining high levels of antioxidant enzymes.

    Effects of BTH, SA, and SiO2 Treatment on Disease Resistance and Leaf HRGP and Lignin Contents of Melon Seedlings
    CHEN Nian-lai,HU Min,QIAO Chang- ping,NAI Xiao-ying,WANG Rui
    Scientia Agricultura Sinica. 2010, 43(3):  535-541 .  doi:10.3864/j.issn.0578-1752.2010.03.013
    Abstract ( 976 )   PDF (252KB) ( 973 )   Save
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    【Objective】 The objective of the experiment was to investigate the inducing effects of benzothiadiazole (BTH), salicylic acid (SA) and nanometer silicon (SiO2) spraying on disease resistance of melon to powdery mildew and its relationship to hydroxyproline-rich glycoprotein(HRGP) and lignin contents in leaves. 【Method】 Two melon cultivars with different powder mildew susceptibilities, Yindi and Kalakesai, were employed as materials. At the fifth day after BTH, SA and SiO2 spraying, seedlings were inoculated with Sphaerotheca fuliginea, and disease indexes, leaf lignin and HRGP contents were determined for four times at alternate days. 【Result】 BTH and SA spraying significantly decreased disease indexes of powdery mildew in melon seedlings especially by BTH spraying and on the resistant cultivar, while SiO2 spraying showed a significant effect only in the initial days after treatment. Leaf lignin and HRGP content increased significantly and systemically in melon seedlings induced by Sphaerotheca fuliginea inoculation and BTH, SA spraying, while SiO2 had no such effect. The accumulation of lignin and HRGP in leaf cell wall was synchronous obviously, and was higher in the resistant cultivar than in the susceptible one. 【Conclusion】 The accumulation of HRGP and the deposition of lignin in cell wall of melon leaves are related to its resistant reactions to powdery mildew, and it is one of the important biochemical mechanisms in melon-Sphaerotheca fuliginea interactions.

    Genetic Relationship of Chaenomeles Cultivars Revealed by SRAP Analysis
    WANG Ming-ming,CHEN Hua-bang,WANG Jian-hua,SONG Zhen-qiao,LI Sheng-bo
    Scientia Agricultura Sinica. 2010, 43(3):  542-551 .  doi:10.3864/j.issn.0578-1752.2010.03.014
    Abstract ( 862 )   PDF (590KB) ( 1012 )   Save
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    【Objective】 The botanical source, genetic relationship, and genetic diversity of Chaenomeles cultivars were probed. The aim is to provide a scientific basis for Chaenomeles cultivars classification. 【Method】 The cluster analysis, principal coordinate analysis and genetic diversity of 27 cultivars of Chaenomeles and 5 wild species were analyzed using 22 SRAP primer combinations.【Result】 A total of 152 polymorphic loci were detected among these materials with average 6.91 polymorphic loci per SRAP primer combination, and the percentage of polymorphic loci was 73.08%. The result of cluster analysis revealed that 32 materials could be divided into 4 groups, namely C. cathayensis system, C. thibetica, C. speciosa system and C. japonica system. C. thibetica appeared to be rather closely related to C. cathayensis system. C. japonica system and C. speciosa system clustered into together. C. japonica system and C. cathayensis system were the most distantly related systems. C. speciosa system took an intermediate position between C. japonica system and C. cathayensis system. Genetic diversity analysis showed that C. japonica system and C. speciosa system were more diverse than C. cathayensis system. Differences in mating and breeding systems among the species can be suggested as a possible explanation of the results. At the genus level, the coefficient of genetic differentiation among species (GST) was 0.4969. 【Conclusion】 SRAP molecular markers was an effective research tool for genetic relationship of Chaenomeles cultivars. Integrated morphological characters and SRAP analysis, the state of basal style hairiness was one of the accurate indicators for identification of botanical source of Chaenomeles cultivars. C. × superba was closely related to C. speciosa, and could be used as a cultivar group in C. speciosa.

    Phylogeny and Genetic Diversity of Annulohypoxylon spp. Paired with Cultivated Tremella fuciformis Berk.
    WEN Wen-ting,JIA Ding-hong,GUO Yong,SUN Qun,YANG Zhi-rong,PENG Wei-hong
    Scientia Agricultura Sinica. 2010, 43(3):  552-558 .  doi:10.3864/j.issn.0578-1752.2010.03.015
    Abstract ( 971 )   PDF (368KB) ( 853 )   Save
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    【Objective】 The aim of this paper is to study the classification and genetic diversity of Xianghui and to provide biological information on utilization and breeding of Xianghui. 【Method】 The phylogeny of 16 strains of Xianghui, which were collected from different provinces and paired with cultivated Tremella, was determined by ITS sequences analysis. Furthermore, the genetic diversity of these strains was analyzed by inter-simple sequence repeat (ISSR). 【Result】 The ITS sequences of the 16 strains were analyzed and the phylogenic tree was constructed, and the results revealed that the homology between Xianghui and Annulohypoxylon stygium was 99%. Among the 70 ISSR primers, 11 with high polymorphism were selected and a total of 62 ISSR markers were obtained. At 0.7 similarity distance, cluster analysis revealed the existence of five distinct genetic groups. 【Conclusion】 The 16 strains of Annulohypoxylon spp. investigated showed low genetic similarity and genetic variations at DNA level significantly.

    STORAGE·FRESH-KEEPING·PROCESSING
    Factors Influencing Nitric Oxide Release from Spermine/Nitric Oxide Adduct and Absorption Characteristics of Nitric Oxide by Horticultural Products
    ZHANG Li-li,ZHAO Li-li,ZHOU Jie
    Scientia Agricultura Sinica. 2010, 43(3):  559-564 .  doi:10.3864/j.issn.0578-1752.2010.03.016
    Abstract ( 1148 )   PDF (237KB) ( 714 )   Save
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    【Objective】 The factors influencing nitric oxide release from spermine/nitric oxide adduct (SPER/NO) and the absorption characteristics of nitric oxide by horticultural products were investigated, which is useful for the application of SPER/NO to the storage of horticultural products. 【Method】 SPER/NO was synthesized, and the effects of medium, humidity and additives on the release rate of nitric oxide from SPER/NO, as well as the absorption characteristics of nitric oxide by horticultural products were investigated by an electrochemical sensor. 【Result】 SPER/NO was found to release nitric oxide rapidly in acidic media and high humidity, which was delayed by the starch added as a filler. Nitric oxide release from SPER/NO was absorbed rapidly by horticultural products, and the rate of absorption in nitrogen was higher than that in air. 【Conclusion】 In high humidity atmosphere, a mixture of SPER/NO and citric acid was found to generate nitric oxide which was absorbed rapidly by horticultural products.

    Identification of Hapten-Carrier Protein Conjugate by Three Electrophoresis Methods
    LEI Hong-tao,PANG Jie,HE Li-ping,YANG Jin-yi,SUN Yuan-ming,QIN Yi-lan,LIANG Ming-biao
    Scientia Agricultura Sinica. 2010, 43(3):  565-570 .  doi:10.3864/j.issn.0578-1752.2010.03.017
    Abstract ( 901 )   PDF (400KB) ( 817 )   Save
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    【Objective】To establish a simple electrophoresis characterization method for hapten-carrier protein bioconjugate. 【Method】 SDS-PAGE, nondenaturing agarose gel electrophoresis and high performance capillary electrophoresis were employed and compared with spectrum method. 【Result】 The result showed that SDS-PAGE was not a sensitive candidate method to differentiate carrier protein from bioconjugate when both proteins have less molecular weight difference, but nondenaturing gel electrophoresis was a better alternative, which could discriminate the free carrier protein and bioconjugate protein with different net charge numbers. High performance capillary zone electrophoresis was another powerful approach for hapten-carrier protein bioconjugate characterization. The information obtained from three electrophoresis was in accordance with the one from ultraviolet- visible spectrum scanning. 【Conclusion】 Three electrophoresis methods had different advantages and disadvantages, cost-saving and simple nondenaturing electrophoresis seemed to have more availability in most laboratories.

    Effects of High Energy Electron Beam on Physiological Quality of Kyoho Grape
    WANG Qiu-fang,QIAO Yong-jin,QIAO Xu-guang
    Scientia Agricultura Sinica. 2010, 43(3):  571-577 .  doi:10.3864/j.issn.0578-1752.2010.03.018
    Abstract ( 1130 )   PDF (328KB) ( 833 )   Save
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    【Objective】 The preservation effects of high energy electron beam on Kyoho grapes were studied for providing a theoretical and practical basis of application in the fruits and vegetables storage field. 【Method】 The Kyoho grapes were put in cold storage of -0.5—0.5℃, RH 85%—95% after irradiation of 400 Gy, 700 Gy, 1 000 Gy, 1 500 Gy, and 2 500 Gy and the physiological quality was studied during storage. 【Result】 The results showed that the dosages of 400 Gy, 700 Gy, and 1 000 Gy could control the decline of ascorbic acid and tannin content effectively, maintain higher activity of superoxide dismutase(SOD), peroxidase(POD), catalase(CAT) enzymes, restrain the degree of membrane lipid peroxidation with low temperature. The treatment of 700 Gy could maintain lower rotting rate and shattering rate. The VC content was 3.79 mg/100 g and the activity of SOD and POD were 14.89 U?g-1 and 40.93 U?g-1?min-1, which were higher than the other groups observably (P<0.05). However, the treatments of 1 500 Gy and 2 500 Gy had undesirable effects on product quality. The content of VC and tannin decreased rapidly, and the phenomenon of shattering was more serious than other groups. 【Conclusion】 The Kyoho grape could keep its physiological quality and fresh-keeping effect at the best state under 700 Gy irradiation. The storage quality was improved obviously compared with the control group preserved for 98 days at -0.5—0.5℃.

    ANIMAL SCIENCE·RESOURCE INSECT
    Effects of Transportation Stress on Expressions of IL-2, IL-6 and IL-10 mRNAs in Spleen and Regulation of Cytokines in Pigs
    Lü Qiong-xia,ZHANG Shu-xia,ZHAO Ru-qian
    Scientia Agricultura Sinica. 2010, 43(3):  578-584 .  doi:10.3864/j.issn.0578-1752.2010.03.019
    Abstract ( 918 )   PDF (405KB) ( 681 )   Save
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    【Objective】 The objective of this study was to explore expressions of IL-2, IL-6, IL-10 mRNAs, IL-2RmRNA and IL-6RmRNA in spleen and regulation of cytokines by P38MAPK-NF-?B in pigs during truck transportation. 【Method】 Nineteen pigs were randomly divided into 4 groups for 0 h, 1 h, 2 h and 4 h transportaion when mean live weight was approximately (50±2) kg (mean ± standard deviation; SD). At the end of the transportation trial, the spleen was removed and frozen in liquid nitrogen. SYBR Green I-based one-step real-time quantitative RT-PCR amplification was performed in quantitation of the three cytokines, IL-2RmRNA and IL-6RmRNA. P38MAPK in the homogenization sample of spleen was measured with homologous specific enzyme-linked immunosorbent assay (ELISA). The distribution of NF-?B in the spleen was examined using confocal laser scanning microscope (CLSM). 【Result】 After 1 h, 2 h and 4 h of transportation, the expression level of IL-6, IL-10 and IL-6RmRNAs in spleen were significantly changed compared with the control group during transportation stress. NF-?B in the spleen has a sharp rise at 1 h, followed by a slight decline, rise again at 4 h and higher than the other three groups. The rates of NF-?B entry nuclear showed a similar trend, but there was no significant change with respect to P38MAPK. 【Conclusion】 The IL-6mRNA, IL-10mRNA and the expression of IL-6RmRNA could be significantly changed by transportation stress and were also related to the time of post-stress in pigs. Correlation analysis showed that the spleen is an important regulator of IL-6 levels among the detected three kinds of cytokines during transportation stress. Combination of expression and regulation of cytokine signaling mechanism shows that the activation of NF-?B in the spleen may be play an important role in the peripheral immune-related cytokines change in transport-stressed pigs.

    Advances in Research on Classification and Major Species of Bee Mites
    LUO Qi-hua,ZHOU Ting,WANG Qiang,DAI Ping-li,WU Yan-yan
    Scientia Agricultura Sinica. 2010, 43(3):  585-593 .  doi:10.3864/j.issn.0578-1752.2010.03.020
    Abstract ( 1231 )   PDF (361KB) ( 1807 )   Save
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    The mites that parasitize honey bees have become important pests in the world. With the increasing damage to the beekeeping and fluvalinate-resistant mites have appeared, the mites have attracted more and more attention. This paper described the progress in study of the mites. ① The species of bee mites is reviewed; ②The distribution,biology, damage of Acarapis woodi, Varroa destructor and Tropilaelaps spp. are summarized, along with control methods; ③Mite-associated bee pheromone; ④Mite-associated bee diseases have also been reviewed. Finally, general direction of research on bee mites are analyzed and discussed.

    VETERINARY SCIENCE
    Study on the Expression and Bioactivity of Chicken Interferon α /Chicken Interleukin-2 Fusion Protein in vivo and in vitro
    YAN Ruo-qian,XIE Cai-hua,WU Zhi-ming,ZHANG Zhi-ling,LIU Guang-hui,LIU Mei-fen
    Scientia Agricultura Sinica. 2010, 43(3):  594-604 .  doi:10.3864/j.issn.0578-1752.2010.03.021
    Abstract ( 945 )   PDF (535KB) ( 716 )   Save
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    【Objective】 The aim of this study was to explore a high efficient chicken gene engineering antiviral agent to prevent and control the chicken viral disease. 【Method】 The recombinant chimeric gene of ChIFN-α-linker-ChIL-2 constructed by chicken interferon α ( ChIFN-α) gene linked chicken interleukin-2 (ChIL-2) gene via a glycine-rich linker by SOE-PCR (splicing by overlap extension-PCR) method was cloned into pGEM-T Easy vector and subsequently sub-cloned into prokaryotic expression vector pQE30. The recombinant ChIFN-α-linker-ChIL-2 (rChIFN-α-linker-ChIL-2) protein was expressed in E.coli JM109 and purified with the innovated recombinant fusion protein purification method and denatured by 8 M urea, refolded by a self-innovative renaturation buffer and dialyzed by PBS buffer, etc. The antiviral bioactivities of rChIFN-α-linker-ChIL-2 protein were tested by inhibiting the 50 percent appearance of cytopathic effect (CPE) of vesicular stomatitis virus (VSV) and infectious bursal disease virus (IBDV) on chicken embryo fibroblast (CEF) cell lines. The ChIL-2 bioactivities of rChIFN-α-linker-ChIL-2 protein were estimated by the method of chicken IL-2 ELISA assay for detection of the specific immunoactivity of ChIL-2 protein. The antivirus bioactivity of rChIFN-α-linker-ChIL-2 protein was evaluated by inhibiting the reproduction of NDV and AIV (H9N2) in chicken embryos, respectively. The bioactivity of rChIFN-α-linker-ChIL-2 protein in chicken was estimated by detecting the NDV hemagglutination inhibition (HI) antibody titer induced by NDV oil-adjuvant inactivated vaccine and attenuated live vaccine, respectively. 【Result】 The chimeric gene of ChIFN-α-linker-ChIL-2 was successfully constructed and cloned into pGEM-T Easy vector, respectively. The rChIFN-α-linker-ChIL-2 protein was abundantly fusion expressed in E.coli and successfully purified with a molecular mass of about 35.9 kD and purity more than 96% on SDS-PAGE, which indicated that the correct rChIFN-α-linker-ChIL-2 fusion protein had been obtained. The antiviral activity units of rChIFN-α-linker-ChIL-2 protein inhibiting the reproduction of VSV and IBDV on CEF cell line were 1.14×108IU?mg-1 and 1.59×106 IU?mg-1 ,respectively, which were much higher than that of the recombinant ChIFN-α protein (rChIFN-α) of control (4.09×106 IU?mg-1, 2.78×105 IU?mg-1). The rChIFN-α-linker-ChIL-2 protein showed the ability of the specific immune response to monoclonal antibody (MAb) of ChIL-2 by ELISA assay, which was similar to the bioactivity of recombinant ChIL-2 protein (rChIL-2) of control. The rChIFN-α-linker-ChIL-2 protein with 200 international unit of IFN-α could obviously decrease the death rate and haemorrhage degree as well as prolong the living time of chicken embryos inoculated by NDV and AIV H9N2, whereas the excessive dose of rChIFN-α-linker-ChIL-2 protein displayed the lower ability of decreasing the death rate and haemorrhage degree of chicken embryos. The rChIFN-α-linker-ChIL-2 protein with appropriate dose exhibited the prominent abilities of antivirus and immunopotentiation in chicken.【Conclusion】 The results of study indicated that the rChIFN-α-linker-ChIL-2 protein had the duplex bioactivity of ChIFN-α protein and ChIL-2 protein on cells, in chicken embryos and chicken, thus laid a foundation for further widely use of rChIFN-α-linker-ChIL-2 protein acting as a main ingredient of chicken gene engineering antiviral agent to prevent and control the chicken viral diseases.
    Relative Quantification of mRNA Transcription of αv, β3, and β6 Subunits Relevant to FMDV Receptors in Different Tissues of Hostein Heifer by Real-time Quantitative RT-PCR
    DU Jun-zheng,CHANG Hui-yun,XUE Shuang,GAO Shan-dian,CONG Guo-zheng,SHAO Jun-jun,LIN Tong,BAO Hui-fang,CAI Xue-peng
    Scientia Agricultura Sinica. 2010, 43(3):  605-611 .  doi:10.3864/j.issn.0578-1752.2010.03.022
    Abstract ( 1195 )   PDF (568KB) ( 702 )   Save
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    【Objective】 To establish a method for the detection of mRNA transcription of αv, β3, β6 subunits by real-time quantitative RT-PCR and analyze the transcription profiles of αvβ3 and αvβ6 as FMDV receptors in different tissues of Hostein heifer.【Method】 According to the sequences of αv, β3, and β6 cDNAs , the real-time PCR primers were designed and synthesized. The △△Ct relative quantification method was used to detect the transcription levels of αv, β3, and β6 mRNAs in 24 different tissues of Hostein heifer.【Result】Results of the study showed that the αv and β3 mRNA were transcripted at high or low level in the 24 different tissues. In constrast, the β6 mRNA is only restricted in the partial tissues, and at high level in the thyroid, hard palate, nasal epithelium, laryngeal, lung, esophageal, kidney and at moderate level in the lip skin, soft palate, tracheal, heart , rumen, rectum, coronary band of front leg, and not detected in the salivary gland, neck lymph node, liver, speen and musle. The distribution pattern is remarkably similar to the tissue tropism of FMDV. The results suggested that the αvβ6 may serve as the major receptor that determines the tissue tropism of FMDV. The tissue distribution pattern of αvβ3 seems to be unrelated to the tissue tropism of FMDV, but the role for αvβ3 as a receptor during FMDV infection can not be ruled out completely. 【Conclusion】 The △△Ct relative quantitative RT-PCR method was established successfully for the detection of mRNA transcription of αv, β3 and β6 subunits.

    RESEARCH NOTES
    Isolation and Characterization of a Blue Copper Protein Gene (GhBCP1) Associated with Cotton Fiber Development
    TIAN Qin,LI Yan-jun,GUO Fang,ZHANG Xin-yu,WANG Hai-yun,SUN Jie
    Scientia Agricultura Sinica. 2010, 43(3):  612-617 .  doi:10.3864/j.issn.0578-1752.2010.03.023
    Abstract ( 932 )   PDF (575KB) ( 728 )   Save
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    【Objective】 The aim of this study was to isolate the cDNA sequence of the gene GhBCP1, and to probe the functions of the gene by characterizing it’s expression patterns in different tissues and developing fibers. 【Method】 The full-length cDNA sequence of GhBCP1 was cloned by Fluorescence differential display (FDD) technique and rapid amplification of the cDNA ends (RACE). Bioinformatics methods were used to analyze the cDNA and the encoded amino acid sequence, and fluorescence real time quantitative PCR method was used to study the expression pattern of the gene in different tissues. 【Result】 A full-length cDNA that is specifically expressed in cotton fiber cells was cloned. The gene was designated GhBCP1 (GeneBank accession: EF222282). The cloned cDNA was 721bp in length which encoded a peptide of 176 amino acids. Blast analysis indicated that this gene encoded a blue copper protein. Southern blot analysis showed that the upland cotton genome contains two copies of the gene. Real-time PCR analysis indicated that GhBCP1 was specifically expressed in fiber cells and its transcripts were more abundant at the transition between the elongation and secondary cell wall synthesis stages. 【Conclusion】 The results showed that GhBCP1 may play a role at the onset of the secondary cell wall synthesis in developing cotton fiber cells.

    Molecular Cloning and Sequence Analysis of Two cDNA Genes of Two New Lepidoptera OR83b Orthologue Chemoreceptors
    XIU Wei-ming,ZHANG Yi-fan,YANG Dian-lin,DONG Shuang-lin,LIU Yu-sheng
    Scientia Agricultura Sinica. 2010, 43(3):  618-625 .  doi:10.3864/j.issn.0578-1752.2010.03.024
    Abstract ( 877 )   PDF (1016KB) ( 725 )   Save
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    【Objective】 The aim of this study was to isolate the cDNA full sequence of an unusually highly conserved olfactory receptor (orthologue to the Drosophila melanogaster DOR83b) gene from the antennae of insects. 【Method】 The olfactory receptor genes were cloned by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. Bioinformatics methods were used to further analyze cDNA sequence obtained and putative amino acid sequence. 【Result】 Two cDNA full sequences of olfactory receptor (OR) gene were amplified from the antennae of male Spodoptera exigua and S. litura, and they were named SexiOR2 and SlitOR2. SexiOR2 and SlitOR2 contained 1 906 bp and 2 483 bp, and with deduced amino acid sequences of 473 residues. The sequence analysis indicated that the deduced amino acid sequences of the cDNA sequences shared high identity with OR83b orthologue chemoreceptor sequences from other previously reported moths. 【Conclusion】 It is inferred that the sequences obtained are cDNA sequences of OR83b orthologue chemoreceptor gene.

    Risk Analysis of the Alien Invasive Gray Pineapple Mealybug (Dysmicoccus neobrevipes Beardsley) in China
    QIN Zhen-qiang,WU Jian-hui,REN Shun-xiang,WAN Fang-hao
    Scientia Agricultura Sinica. 2010, 43(3):  626-633 .  doi:10.3864/j.issn.0578-1752.2010.03.025
    Abstract ( 1098 )   PDF (219KB) ( 850 )   Save
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    【Objective】 The risk of alien invasive gray pineapple mealybug (Dysmicoccus neobrevipes) Beardsley, in China was analyzed. 【Method】 Based on the rule of pest risk analysis (PRA) of international standards for pest measurements (ISPM), quantitative and qualitative analysis was conduced on the risk level of D. neobrevipes in five aspects: distribution, potential damage level, economic value of damaged host plants, spreading probability and risk-management difficulty in China. 【Result】 The synthetic index of the pest risk in China is estimated to be 2.12. 【Conclusion】 The result indicats that D. neobrevipes is a dangerous alien species with high risk in China.

    Design of Weather-Based Indemnity Indices for Paddy Rice Heavy Rain Damage Insurance
    LOU Wei-ping,WU Li-hong,YAO Yi-ping
    Scientia Agricultura Sinica. 2010, 43(3):  632-639 .  doi:10.3864/j.issn.0578-1752.2010.03.026
    Abstract ( 1148 )   PDF (286KB) ( 1029 )   Save
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    【Objective】 The objective of this research was to design weather-based indemnity indices for paddy rice heavy rain damage insurance and to provide technology for policy-guided agricultural insurance. 【Method】 Based on the relationship between yield loss rate of paddy rice caused by heavy rain and meteorology factors and land surface factors, a model of yield loss rate of paddy rice caused by heavy rain was established. With the GIS technology, yield loss rate in a variety of complex terrain was determined. Weather-based indemnity indices for paddy rice heavy rain damage insurance was designed based on the advantage of area yield crop insurance and weather index insurance which refined to a village and a group of villagers level. 【Result】Weather-based indemnity indices for paddy rice heavy rain damage insurance was defined for a village and a group of villagers. With the data, including processes of different typical rainfall and Krosa typhoon (No.0716), the model of single-cropping rice yield loss rate caused by heavy rain was estibalished. The results showed that this model was valid to simulate the distribution of yield loss rate of single-cropping rice sufferring the heavy rian disaster in complicated terrain and could supply a highly believable standard evaluation about the heavy rain disaster loss of single cropping rice. When the yield loss rate of a single-cropping rice with the model was not bigger than 30%, both the index of indemnity and the indemnity ratio were zero. However, as the yield loss rate was bigger than 30%, both the index of indemnity and the indemnity ratio were equal to the yield loss rate. 【Conclusion】 Based on the precipitation and wind speed, the model of single-cropping rice yield loss rate caused by heavy rain could calculated the yield loss rate of single-cropping rice sufferring the heavy rian disaster in the villages of Yuhang. The weather-based indemnity indices was designed in combination with the advantages of area yield crop insurance and weather index insurance. To some extent, it was helpful to eliminate the malady of traditional agricultural insurance at present in China.

    Cloning and Activity Determination of Fatty Acid Synthase (FAS) Gene Promoter of Xinong Saanen Dairy Goat
    ZHANG Xiao,LUO Jun ,LI Jian-hua,ZHAO Wang-sheng,WANG Wei
    Scientia Agricultura Sinica. 2010, 43(3):  640-647 .  doi:10.3864/j.issn.0578-1752.2010.03.027
    Abstract ( 893 )   PDF (465KB) ( 663 )   Save
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    【Objective】 The fatty acid synthase (FAS) gene promoter of Xinong Saanen dairy goat was cloned and sequenced to analyze the active region, thus providing an evidence for function determination and expression regulation of FAS gene. 【Method】 According to the homologous sequences of bovine and homo, and to 5‘UTR of Xinong Saanen dairy goat FAS gene, upstream and downstream primers were designed, respectively. Using genome DNA as the template, the FAS gene promoter was cloned. Primers were redesigned according to the results of bioinformatics analysis, the FAS gene promoter was cloned by segmenting and linking up with the PGL-3 expression vector, co-transfecting 293 and MCF-7 cells with the β-galactosidase control vector, and luciferase and β-galactosidase expression were detected. 【Result】 There are 2 640 bp in FAS gene promoter totally, the homology with bovine and homo is over 90%. The promoter contains several potential transcription factor bind sites such as SP1, Ets, LSF bind sites, several CCAAT boxes and a GC box. The core promoter sequences potentially locate in -1 040—-340 bp, and reduced the range to -721—-540 bp by promoter deletion experiment. 【Conclusion】 The full length of Xinong Saanen Dairy Goat FAS gene promoter was cloned, the potential negative control element was analyzed, and the core promoter sequences were found.

    Expression, Tissue Distribution and Subcellular Localization of Bm595 from Silkworm (Bombyx mori)
    WANG Shuai-yu,SHENG qing,Lü Zheng-bing,CHEN Jian,NIE Zuo-ming,WANG Dan,LIU Li-li,SHEN Hong-dan,SHU Jian-hong,CHEN Jian-qing,WU Xiang-fu,ZHANG Yao-zhou
    Scientia Agricultura Sinica. 2010, 43(3):  648-654 .  doi:10.3864/j.issn.0578-1752.2010.03.028
    Abstract ( 950 )   PDF (524KB) ( 702 )   Save
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    【Objective】 In screening the pupa cDNA library, a novel gene Bm595 was identified, the tissue distribution and localization of Bm595 were analyzed in silkworm. 【Method】 The recombinant plasmid was transformed into E.coli BL 21 which were induced with IPTG. The purified fusion protein was used to immune a rabbit and the polyclonal antibody was prepared. The tissue distribution of Bm595 was analyzed by real-time PCR and Western blot. 【Result】 The quantitative real-time PCR results indicated that the transcription level of Bm595 was the highest in the fifth instar larva and the lowest in egg. The quantity of Bm595 transcription from high to low was testis, silk gland, gut, head, ovary, fat body, epidermis, and air tube, respectively. Western blot results showed that Bm595 was highly expressed in testis, moderately expressed in silk gland and head. Bm595 also existed in fat body. Bm595 was mainly localized in nucleus by immunocytochemistry. 【Conclusion】 A preliminary analysis on Bm595 was made at the mRNA, protein expression and cell level which laid a good foundation for the further studies on Bm595 function.
    Analysis of Differential Proteins of Cucumber Cotyledonary Nodes Induced by PP333
    LI Feng-yu,PAN De-zhuo,LIANG Hai-man,CHEN Wei
    Scientia Agricultura Sinica. 2010, 43(3):  655-660 .  doi:10.3864/j.issn.0578-1752.2010.03.029
    Abstract ( 883 )   PDF (381KB) ( 747 )   Save
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    【Objective】 The aim of the study was to understand and find differential proteins induced by PP333, which could provide valuable information for further study on its effective law and mechanism. 【Method】 Two-dimensional electrophoresis (2-DE) was used to study the variation expression of proteins of cucumber cotyledonary nodes induced with PP333. The characteristics and functions of differentially expressed proteins were analyzed with MALDI-TOF-TOF/MS and Protein Data Bank. 【Result】 Nearly 1 000 proteins were reproducibly detected in 2-DE gel. Five differential proteins were identified, including 14-3-3 protein (R2), aminopeptidase (R7), methionine aminopeptidase (R11), 60s acidic ribosomal protein (R20) and PEPC (R21). The proteins were closely related to metabolism, translation and signal transduction, which may play an important role in flower bud differentiation of cucumber. 【Conclusion】 PP333 treatment induced the variation of many proteins which regulated plant growth and development by their mutual coordination.